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  2. SnapTag-Binder

    Plasmid
    #179142
    Purpose
    Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9). Binder binds to the 7 a.a. peptide SsrA (AANDENY).
    Depositor
    Klaus Hahn
    Article
    Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.
    Insert
    SspB
    Use
    Tags
    SNAP-tag
    Expression
    Mammalian
    Mutation
    Y11K/A15E, residue numbering from pdb: 1ou9
    Promoter
    Available since
    Jan. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    179142-plasmid-map-sequence-id-353405

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  8. pDEST-Spe4-Kozak-Snaptag-RfA

    Plasmid
    #186385
    Purpose
    Destination vector for the expression of a fluorescent fusion of N-ter Snap tag and a protein of interest into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    186385-plasmid-map-sequence-id-373828

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  9. pDEST-Spe4-RfA-Snaptag-stop

    Plasmid
    #186395
    Purpose
    Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter Snap tag into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    186395-plasmid-map-sequence-id-373843

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  12. StarPort01

    Plasmid
    #164503
    Purpose
    To generate a stable acceptor cell line with a BxbI attP site at the AASV1 locus, between an exogenous human pRPL30 promoter and a tripartite V5/SNAPtag/Blast reporter.
    Depositor
    Bo Huang
    Insert
    V5-SNAPtag-PDGFR_TM-T2A-Blast
    Use
    To generate attp acceptor cell lines for bxbi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human RPL30
    Available since
    Availability
    Academic Institutions and Nonprofits only
    164503-plasmid-map-sequence-id-324720

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  13. StarPort02

    Plasmid
    #164504
    Purpose
    To generate a stable acceptor cell line with an improved BxbI attP-GA site at the AASV1 locus, between an exogenous human pRPL30 promoter and a tripartite V5/SNAPtag/Blast reporter.
    Depositor
    Bo Huang
    Insert
    V5-SNAPtag-PDGFR_TM-T2A-Blast
    Use
    To generate attp-ga acceptor cell lines for bxbi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human RPL30
    Available since
    Availability
    Academic Institutions and Nonprofits only
    164504-plasmid-map-sequence-id-324721

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  15. SNAP-LC3 HRD

    Plasmid
    #207545
    Purpose
    Homologous recombination donor to integrate a SNAP-Tag at the endogenous LC3 locus in human cells
    Depositor
    Jens Schmidt
    Article
    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007
    Insert
    SNAPtag with internal PuroR cassette flanked by human LC3B locus sequences
    Use
    Tags
    SNAPtag
    Expression
    Mammalian
    Mutation
    Promoter
    Endogenous
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    207545-plasmid-map-sequence-id-430987

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Showing: 1 - 37 of 37 results