Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 143 results for: PCR2

Showing: 1 - 50 of 143 results

  1. pCR2.1_HBB_N

    Plasmid
    #87847
    Purpose
    pCR2.1-based plasmid holding a 309-bp fragment containing the exon-1-exon-2 splice border of normal human β-globin mRNA
    Depositor
    Marina Kleanthous
    Article
    Patsali et al Int J Mol Sci. 2020 Sep 11;21(18). pii:
    Insert
    Homo sapiens hemoglobin subunit beta (HBB), Normal cDNA fragment (Exon1/2) (HBB Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    87847-plasmid-map-sequence-id-313714

    Enlarge

  4. pCR2.1_HBB_A

    Plasmid
    #87848
    Purpose
    pCR2.1-based plasmid holding a 328-bp fragment containing the exon-1-exon-2 splice border of aberrant HBB[IVSI-110(G>A)] human β-globin mRNA
    Depositor
    Marina Kleanthous
    Article
    Patsali et al Int J Mol Sci. 2020 Sep 11;21(18). pii:
    Insert
    Homo sapiens hemoglobin subunit beta (HBB), Aberrant cDNA fragment (Exon1/19nt mispliced Intron1 + Exon2) (HBB Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    HBB(IVSI-110) β-thalassaemia misplicing mutation …
    Promoter
    Available since
    Oct. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    87848-plasmid-map-sequence-id-313713

    Enlarge

  5. pCR2.1-InC

    Plasmid
    #83959
    Purpose
    Contains a synthetic insert to serve as internal control DNA template for a Salmonella Real-Time PCR assay.
    Depositor
    Kai-Shun Chen
    Insert
    Internal Control sequence
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    83959-plasmid-map-sequence-id-160468

    Enlarge

  6. pCR2.1-myco

    Plasmid
    #36870
    Depositor
    Mario Chin
    Insert
    myco
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 20, 2012
    Availability
    Academic Institutions and Nonprofits only
    36870-plasmid-map-sequence-id-46090

    Enlarge

  7. pCR2.1-hASIC1

    Plasmid
    #32790
    Depositor
    Cecelia Canessa
    Insert
    ASIC1 (ASIC1 Human)
    Use
    Cloning
    Tags
    Expression
    Mutation
    Promoter
    plac
    Available since
    Nov. 1, 2011
    Availability
    Academic Institutions and Nonprofits only

  10. pCR2.1-ggASIC1

    Plasmid
    #32791
    Depositor
    Cecelia Canessa
    Insert
    ASIC1 (ASIC1 Chicken)
    Use
    Cloning
    Tags
    Flag
    Expression
    Mutation
    Promoter
    plac
    Available since
    Nov. 1, 2011
    Availability
    Academic Institutions and Nonprofits only

  11. pCR2.1-PBGD

    Plasmid
    #36869
    Depositor
    Mario Chin
    Insert
    PBGD fragment (HMBS Human)
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 20, 2012
    Availability
    Academic Institutions and Nonprofits only
    36869-plasmid-map-sequence-id-46087

    Enlarge

  12. pCR2.1-p55alpha

    Plasmid
    #1409
    Depositor
    Lewis Cantley
    Insert
    PI3K regulatory subunit p55alpha (Pik3r1 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 16, 2005
    Availability
    Academic Institutions and Nonprofits only

  25. TC9pAB

    Plasmid
    #71310
    Purpose
    used as a template for in vitro transcription of Cas9 mRNA after linearizing with BbsI
    Depositor
    Karl Degenhardt
    Insert
    Cas9
    Use
    In vitro transcription
    Tags
    NLS
    Expression
    Mutation
    Promoter
    T7
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    71310-plasmid-map-sequence-id-129410

    Enlarge

  26. TC9nickpAB

    Plasmid
    #71311
    Purpose
    used as a template for in vitro transcription of Cas9 nickase mRNA after linearizing with BbsI
    Depositor
    Karl Degenhardt
    Insert
    Cas9 nickase
    Use
    In vitro transcription
    Tags
    NLS
    Expression
    Mutation
    D10A nickase
    Promoter
    T7
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    71311-plasmid-map-sequence-id-131863

    Enlarge

  27. GPR12-DuET

    Plasmid
    #213260
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GPR12 (GPR12 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213260-plasmid-map-sequence-id-430810

    Enlarge

  28. CYSLTR2-Tango

    Plasmid
    #66267
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    CYSLTR2 (CYSLTR2 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  29. CYSLTR2-DuET

    Plasmid
    #213226
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CYSLTR2 (CYSLTR2 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213226-plasmid-map-sequence-id-430788

    Enlarge

  30. LPAR1-DuET

    Plasmid
    #213329
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    LPAR1 (LPAR1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213329-plasmid-map-sequence-id-430863

    Enlarge

  31. LHP1034 - ECFP-Nterm

    Plasmid
    #32436
    Depositor
    Linda Hicke
    Insert
    ECFP
    Use
    Cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 21, 2011
    Availability
    Academic Institutions and Nonprofits only

  32. LHP1032 - EYFP-Nterm

    Plasmid
    #32438
    Depositor
    Linda Hicke
    Insert
    EYFP
    Use
    Cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  33. LHP1033 - EYFP-Cterm

    Plasmid
    #32437
    Depositor
    Linda Hicke
    Insert
    EYFP
    Use
    Cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  34. LHP1035 - ECFP-Cterm

    Plasmid
    #32435
    Depositor
    Linda Hicke
    Insert
    ECFP
    Use
    Cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  35. pHMR1B

    Plasmid
    #102958
    Purpose
    gene cloning of MR1B
    Depositor
    Lucia Mori, Gennaro De Libero
    Insert
    MR1B cDNA (MR1P1 Human)
    Use
    Cloning
    Tags
    Expression
    Mutation
    Stop codon has been removed by HindIII site
    Promoter
    Available since
    Nov. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    102958-plasmid-map-sequence-id-198778

    Enlarge

  36. pSaGuide

    Plasmid
    #64710
    Purpose
    Cloning and expression of Staphylococcus aureus guide RNA
    Depositor
    Kiran Musunuru
    Insert
    Staphylococcus aureus guide RNA
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    64710-plasmid-map-sequence-id-108923

    Enlarge

  37. CAMP no 3'UTR

    Plasmid
    #129561
    Purpose
    cytomplasmic marker without terminator (less integration)
    Depositor
    Inaki Ruiz-Trillo
    Insert
    mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    129561-plasmid-map-sequence-id-258978

    Enlarge

  38. Clim-DC1-mCherry-H2B-Venus

    Plasmid
    #129559
    Purpose
    nuclear marker
    Depositor
    Inaki Ruiz-Trillo
    Insert
    mcherry and Histone 2B fused to venus
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    129559-plasmid-map-sequence-id-258976

    Enlarge

  42. Clim Act MCH (CAM)

    Plasmid
    #104443
    Purpose
    Expresses mCherry under endogenous actin promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope.
    Depositor
    Inaki Ruiz-Trillo, Elena Casacuberta
    Insert
    mCherry
    Use
    Expression in corallochytrium (single-celled euka…
    Tags
    Expression
    Mutation
    Promoter
    actin
    Available since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    104443-plasmid-map-sequence-id-200869

    Enlarge

  43. Clim EF1 MCH (CEM)

    Plasmid
    #104444
    Purpose
    Expresses mCherry under elongation factor 1 promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope.
    Depositor
    Inaki Ruiz-Trillo, Elena Casacuberta
    Insert
    mCherry
    Use
    Expression in corallochytrium (single-celled euka…
    Tags
    Expression
    Mutation
    Promoter
    EF1
    Available since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    104444-plasmid-map-sequence-id-200864

    Enlarge

  44. Clim Tub MCH (CTM)

    Plasmid
    #104445
    Purpose
    Expresses mCherry under tubulin promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope.
    Depositor
    Inaki Ruiz-Trillo, Elena Casacuberta
    Insert
    mCherry
    Use
    Expression in corallochytrium (single-celled euka…
    Tags
    Expression
    Mutation
    Promoter
    tubulin promoter
    Available since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    104445-plasmid-map-sequence-id-200865

    Enlarge

Showing: 1 - 50 of 143 results