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We narrowed to 9,280 results for: Pol

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Showing: 1 - 50 of 9280 results

  3. 1073A(HomeR1)=pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]

    Plasmid
    #159676
    Purpose
    Plasmid provides the HomeR#1 gene drive element harboring a rescue, gRNA#1, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #1 via HDR.
    Depositor
    Omar Akbari
    Article
    Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1
    Insert
    pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
    Expression
    Insect
    Available Since
    March 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    159676-plasmid-map-sequence-id-314890

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  4. 1073B(HomeR2) =pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#2(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]

    Plasmid
    #159677
    Purpose
    Plasmid provides the HomeR#2 gene-drive element harboring a rescue, gRNA#2, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #2 via HDR.
    Depositor
    Omar Akbari
    Article
    Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1
    Insert
    [Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#2(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
    Expression
    Insect
    Available Since
    March 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    159677-plasmid-map-sequence-id-314891

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  11. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-T4DNApol (JO638)

    Plasmid
    #208956
    Purpose
    A variant CE1 construct with T4 DNA polymerase (-exo, activity enhanced), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-T4pol-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A), T4Pol(-exo;D189A/E191A/L412M)
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208956-plasmid-map-sequence-id-415745

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  12. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-PolBeta (JO574)

    Plasmid
    #208953
    Purpose
    A variant CE1 construct with human DNA polymerase beta, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-PolBeta-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A)
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208953-plasmid-map-sequence-id-415742

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  25. PSL1180polyUBdsRED

    Plasmid
    #49327
    Purpose
    Donor plasmid for homologous recombination that expresses dsRED constitutively using the Aedes aegypti polyubiquitin (PUb) promoter.
    Depositor
    Leslie Vosshall
    Insert
    dsRED
    Expression
    Insect
    Promoter
    Aedes polyubiquitin
    Available Since
    Nov. 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    49327-plasmid-map-sequence-id-73022

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  36. pOLENTE

    Plasmid
    #80683
    Purpose
    DEST vector for cell-free in vitro expression
    Depositor
    Nico Dissmeyer
    Type
    Empty backbone
    Use
    Synthetic Biology; In vitro transcription and tra…
    Expression
    Mammalian
    Promoter
    T7 and SP6
    Available Since
    Aug. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    80683-plasmid-map-sequence-id-152045

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  37. pT7CFE1_polio_3ABC

    Plasmid
    #212986
    Purpose
    Encodes Poliovirus type I Mahoney 3ABC optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Poliovirus Type I Mahoney 3ABC
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Promoter
    T7
    Available Since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212986-plasmid-map-sequence-id-420355

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  38. POLQA

    Plasmid
    #74645
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    POLQA (POLQ Human)
    Use
    Baculovirus
    Tags
    His-6-TEV
    Promoter
    PH
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    74645-plasmid-map-sequence-id-422680

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  44. pPolI-PA-66

    Plasmid
    #240810
    Purpose
    Expresses influenza A virus mvRNA PA-66
    Depositor
    AJ te Velthuis
    Insert
    mvRNA PA-66
    Expression
    Mammalian
    Available Since
    Aug. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    240810-plasmid-map-sequence-id-484959

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  45. pPolI-PA-60

    Plasmid
    #240809
    Purpose
    Expresses influenza A virus mvRNA PA-60
    Depositor
    AJ te Velthuis
    Insert
    mvRNA PA-60
    Expression
    Mammalian
    Promoter
    Pol I
    Available Since
    Aug. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    240809-plasmid-map-sequence-id-484880

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  49. polySIM

    Plasmid
    #221068
    Purpose
    Expression of polySIM protein under T7 promoter
    Depositor
    Michael Rosen
    Article
    Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33
    Insert
    10 repeats of SUMO interacting motif (SIM) from PIASx each separated by (GGS)4
    Tags
    His and MBP
    Expression
    Bacterial
    Promoter
    unknown
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    221068-plasmid-map-sequence-id-459203

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Showing: 1 - 50 of 9280 results