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Plasmid#91368PurposeProtein expression and purification of human SH3 domain construct GRAP2-2/2DepositorInsertGRAP2-2/2 (GRAP2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GRAP2_SH3
Plasmid#110016PurposeProtein expression and purification of GRAP2_SH3DepositorInsertGRAP2_SH3 (GRAP2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GRAP-2/2
Plasmid#91529PurposeProtein expression and purification of human SH3 domain construct GRAP-2/2DepositorInsertGRAP-2/2 (GRAP Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GRAP2-1/2
Plasmid#91467PurposeProtein expression and purification of human SH3 domain construct GRAP2-1/2DepositorInsertGRAP2-1/2 (GRAP2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GRAP-1/2
Plasmid#91412PurposeProtein expression and purification of human SH3 domain construct GRAP-1/2DepositorInsertGRAP-1/2 (GRAP Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcFLAG-Grap2-WT
Plasmid#74511PurposeOverexpression of Grap2 in mammalian cellsDepositorInsertGrap2 (GRAP2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGBK CUP1 mGrape3
Plasmid#170265PurposeYeast two-hybrid vector using a copper inducible CUP1 promoter, expressing mGrape3DepositorArticleInsertmomeric Grape 3
UseTagsGAL4 DNA Binding Domain and MycExpressionBacterial and YeastMutationPromoterCUP1Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX Grap2-SH2
Plasmid#46435DepositorInsertGrap2 (GRAP2 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 52-159PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Grap-SH2
Plasmid#46434DepositorInsertGrap (GRAP Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 51-160PromoterTacAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcFLAG-Grap2-R83E
Plasmid#74512PurposeOverexpression of Grap2 R83E in mammalian cellsDepositorInsertGrap2 (GRAP2 Human)
UseTagsFLAGExpressionMammalianMutationR83EPromoterCMVAvailable sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGMV-U
Plasmid#112797PurposeUniversal BeYDV-derived vector for cloning of up to four gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGMV-gSWEET4
Plasmid#112796PurposeUniversal BeYDV-derived vector with gRNAs targeting the VviSWEET4 geneDepositorInsertgRNA pair targeting VviSWEET4
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
EF2451_pET21a(+)
Plasmid#13840DepositorInsertPPAT
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJune 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET21a(+)-PA PdxB
Plasmid#12668DepositorInsertErythronate-4-phosphate dehydrogenase
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
TA0474_pET21a(+)
Plasmid#13839DepositorInsertIS200 transposase (Ta0474 Thermoplasma acidophilum)
UseTagsExpressionBacterialMutationSee Depositor Comments BelowPromoterAvailable sinceJune 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
pNGFP-BC
Plasmid#161956PurposeUsed for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).DepositorTypeEmpty backboneUseTagsGFPuv His8ExpressionBacterialMutationPromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pCGFP-BC
Plasmid#161959PurposeUsed for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).DepositorTypeEmpty backboneUseTagsGFPuv His8ExpressionBacterialMutationPromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pNGFP-EU
Plasmid#161967PurposeUsed for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).DepositorTypeEmpty backboneUseTagsEGFP His8ExpressionMammalianMutationPromoterCMVAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pCGFP-EU
Plasmid#161968PurposeUsed for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).DepositorTypeEmpty backboneUseTagsEGFP His8ExpressionMammalianMutationPromoterCMVAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pCAGGS mutated Pdx1 (DM#233)
Plasmid#15072DepositorInsertPdx1
UseTagsExpressionMammalianMutationDNA binding mutantPromoterAvailable sinceOct. 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
His6-TEV-CRBN-midi
Plasmid#215330PurposeCRBN midi construct for use in crystallography and biophysical studies enabling ligand and degrader designDepositorInsertCRBN (CRBN Human)
UseTags6xHis and TEVExpressionBacterialMutationcontains amino acids 41-187 followed by a Gly-Ser…PromoterlacIAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GST-PS-Gads 1-155 GB1 Gads 261-330-Cys (XSB365)
Plasmid#78507PurposeConstruct for expressing Gads SH3, SH2, and SH3 domain in E. Coli, with a C-terminal Cys for labelingDepositorInsertGads SH3 SH2 SH3 domain (GRAP2 Human)
UseTagsGST followed by a prescission protease claevage s…ExpressionBacterialMutationPromoterAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETcon(I-LtrI)
Plasmid#41523DepositorInsertI-LtrI
UseTags3x(G4S) Linker, G3S Linker, HA Tag, and Myc TagExpressionYeastMutationPromoterGAL1Available sinceApril 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET21b-P35
Plasmid#11808DepositorInsertP35
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
PaBphP 1-497
Plasmid#36920DepositorInsertPaBphP-PCD
UseTagsHisExpressionBacterialMutationContains the photosensory core domain coding regi…PromoterT7Available sinceMay 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
EC.SurA.pTYB1.a21-428.wt
Plasmid#12606DepositorInsertEscherichia coli surA (surA E. coli)
UseTagsinteinExpressionBacterialMutationwild typePromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGEM-MNT-1
Plasmid#15462DepositorInsertneurotensin (Nts Mouse)
UseCloning and transcription vectorTagsExpressionMutationPromoterAvailable sinceAug. 24, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBS-NT-AscI
Plasmid#15461DepositorInsertneurotensin (Nts Mouse)
UseTagsExpressionMutationAdded AscI site 3 bp 3' of stop codonPromoterAvailable sinceAug. 24, 2007AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_GP64
Plasmid#207276PurposeMammalian expression of baculovirus GP64 proteinDepositorInsertGP64
UseTagsExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1 mouse PARG (439-959)
Plasmid#132615Purposeexpresses mouse PARG(439-959) in E. coli.DepositorInsertPARG (439-959)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-22b(+)-UTPase
Plasmid#12667DepositorInsertdUTP pyrophosphatase (DUT1 Budding Yeast)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET-22b(+)-RNase HIII
Plasmid#12683DepositorInsertRNase HIII
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pETNb-cALFA
Plasmid#159989PurposeFor the protein expression with ALFA tag at C-terminus in E. coliDepositorTypeEmpty backboneUseTagsHRV3C protease cleavage site-ALFA tag-8histidineExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET16B.Pfu
Plasmid#12509PurposeBacterial expression of Pfu DNA polymeraseDepositorInsertPfu DNA polymerase
UseTagsExpressionBacterialMutationY218H, Q462R, F533L, K559I and V683A (mutations w…PromoterAvailable sinceSept. 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGST2-TSG101 (2-145)
Plasmid#184810PurposeBacterial expression of TSG101 UEV domain (aa 2-145)DepositorInsertTSG101 (TSG101 Human)
UseTagsGSTExpressionBacterialMutationaa 2-145 onlyPromotertacAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETNb-nALFA
Plasmid#159988PurposeFor the protein expression with ALFA tag at N-terminus in E. coliDepositorTypeEmpty backboneUseTagsMVKYI-8histidine-ALFA tag-HRV3C protease cleavage…ExpressionBacterialMutationPromoterAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFBNb-cALFA
Plasmid#159990PurposeFor the protein expression with ALFA tag at C-terminus in insect cellsDepositorTypeEmpty backboneUseTagsHRV3C protease cleavage site-ALFA tag-8histidineExpressionInsectMutationPromoterAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pCAGGS-Chronos-mRuby2-ST
Plasmid#105446PurposeCation channelrhodopsin Chronos fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendritesDepositorInsertChronos-mRuby2-ST
UseTagsmRuby2 and soma targeting motifExpressionMutationPromoterpCAGGSAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
RCAS DNhRARalpha (CC#1865)
Plasmid#15153DepositorInsertRAR alpha (dom neg) (RARA Human)
UseRetroviral; Avian expressionTagsExpressionMutationDominant negative. Truncated at aa403, so lacks t…PromoterAvailable sinceNov. 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET-22b(+)-HpENR
Plasmid#12651DepositorInsertnoyl-acyl carrier protein reductase (fabI Helicobacter pylori)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET21a.MJ0285
Plasmid#11304DepositorInsertsmall heat shock protein (MJ_0285 M. jannaschii)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 10, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET28a-hdNadV
Plasmid#83362PurposeExpresses nadV from Haemophilus ducreyi in Escherichia coliDepositorInsertnadV
UseTagsnoneExpressionBacterialMutationPromoterT7Available sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
TrmD_HaeIn/pET28b(+) N,C-tag
Plasmid#12665DepositorInserttRNA (guanine-N(1)-)-methyltransferase (trmD Haemophilus influenzae)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
RCAS Cyp26 (CC#62)
Plasmid#15154DepositorInsertCyp26 (CYP26A1 Chicken)
UseRetroviral; Avian expressionTagsExpressionMutationPromoterAvailable sinceJune 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
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LDB221
Plasmid#185427PurposeBacterial expression of Nup1 C-terminal residues 777-1076 as GST fusionDepositorInsertNUP1
UseTagsGSTExpressionBacterialMutationNup1 C-terminal residues 777-1076PromoterAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
LDB203
Plasmid#185424PurposeBacterial expression of N-terminus of NUP1 nucleoporin as GST-Nup1-N fusionDepositorInsertNUP1
UseTagsGSTExpressionBacterialMutationNup1 N-terminal domain onlyPromoterAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
LDB205
Plasmid#185425PurposeBacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusionDepositorInsertNUP1
UseTagsGSTExpressionBacterialMutationNup1 C-terminal domain onlyPromoterAvailable sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB101
Plasmid#185064PurposeBacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion truncated after the last FXFG repeatDepositorInsertNUP1
UseTagsGSTExpressionBacterialMutationNup1 C-terminal region, truncatedPromoterAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only