-
Plasmid#63900PurposeBackbone of pComb3H containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control.DepositorInsertTT Fab
UsePhage displayTagsgeneIIIExpressionBacterialMutationPromoterlacZAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHAhTTP
Plasmid#48738PurposeExpresses hTTPDepositorInsertTTP (ZFP36 Human)
UseTagsFLAG, HA, and V5ExpressionMammalianMutationPromoterCMV, 2x TetAvailable sinceJune 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
61427-sgHtt_10
Plasmid#157861PurposeExpresses a sgRNA targeting mouse Htt promoter for gene activation using the SAM systemDepositorInsertHtt (Htt Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
61427-sgHtt_6
Plasmid#157860PurposeExpresses a sgRNA targeting mouse Htt promoter for gene activation using the SAM systemDepositorInsertHtt (Htt Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-hTtAgo
Plasmid#104383Purposehuman codon optimized TtAgo; pCDNA3 vector backbone, mammalian expressionDepositorInserthTtAgo
UseTags3XFlag, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
MR015-PHOX2BRightTALEN_NN
Plasmid#49449PurposeRight TALEN for PHOX2B with NNDepositorInsertTALEN
UseTALENTagsExpressionMammalianMutationPromoterAvailable sinceDec. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
MR015-PHOX2BRightTALEN_NK
Plasmid#49478PurposeRight TALEN for PHOX2B with NKDepositorInsertTALEN
UseTALENTagsExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTreTight-Htt94Q-CFP
Plasmid#23966DepositorInsertN-terminal fragment Huntingtin Q94 (HTT Human)
UseTagsCFPExpressionMammalianMutation94 polyQ repeatPromoterAvailable sinceApril 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV10-hTti1
Plasmid#30213DepositorInsertTel2 interacting protein 1 (TTI1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJuly 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJOP-HTT-HR65Q
Plasmid#92249PurposeHTT donor DNA with 65Q for homologous recombination-mediated gene modificationDepositorInsertHTT donor DNA (HTT Human)
UsePrecisionx hr targeting vectorTagsExpressionMutationBarcode of wobble bases to prevent TALEN cleaving…PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJOP-HTT-HR45Q
Plasmid#92248PurposeHTT donor DNA with 45Q for homologous recombination-mediated gene modificationDepositorInsertHTT donor DNA (HTT Human)
UsePrecisionx hr targeting vectorTagsExpressionMutationBarcode of wobble bases to prevent TALEN cleaving…PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJOP-HTT-HR18Q
Plasmid#87228PurposeHTT HR arms with 18 CAG repeats and piggyBac selection cassetteDepositorInsert1.7kb HTT 5' homology arm, 2.5kb HTT 3' homology arm
UseTagsExpressionMammalianMutationPromoterEF1a driving selection cassetteAvailable sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJOP-HTT-HR81Q
Plasmid#92250PurposeHTT donor DNA with 81Q for homologous recombination-mediated gene modificationDepositorInsertHTT donor DNA (HTT Human)
UsePrecisionx hr targeting vectorTagsExpressionMutationBarcode of wobble bases to prevent TALEN cleaving…PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJOP-HTT-HR30Q
Plasmid#92247PurposeHTT donor DNA with 30Q for homologous recombination-mediated gene modificationDepositorInsertHTT donor DNA (HTT Human)
UsePrecisionx hr targeting vectorTagsExpressionMutationBarcode of wobble bases to prevent TALEN cleaving…PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX335 HTT sgRNA-a
Plasmid#87201PurposepX335 vector encoding SpCas9n and a chimeric guide RNA targeting exon 1 of HTTDepositorInsertHTT (HTT Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
HTT delta-exon1
Plasmid#162274PurposeBaculovirus and mammalian expressionDepositorInsertHTT (HTT Human)
UseBaculovirus expresssionTags10xHis-FlagExpressionMammalianMutationPromoterCMV and P10Available sinceDec. 17, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pX335 HTT sgRNA-b
Plasmid#87200PurposepX335 vector encoding SpCas9n and a chimeric guide RNA targeting 5' UTR of HTTDepositorInsertHTT (HTT Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
USPQ-HTT-TALEN1
Plasmid#92243PurposeTALEN targeting upstream of HTT gene (KKR), forms obligate heterodimer with USPQ-HTT-TALEN2DepositorInsertTALEN
UseTALENTagsExpressionMutationPromoterCMVAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
USPQ-HTT-TALEN2
Plasmid#92244PurposeTALEN targeting upstream of HTT gene (ELD), forms obligate heterodimer with USPQ-HTT-TALEN1DepositorInsertTALEN
UseTALENTagsExpressionMutationPromoterCMVAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWalium Htt19Q-mCherry
Plasmid#201248Purposeallows the expression of the insert in Drosophila melanogasterDepositorInsertHuntingtin (HTT Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cox1-FusXTBE-RightTALE
Plasmid#196503PurposeExpresses right-side TALE–DddAtox C term half cox1-FusXTBE in zebrafishDepositorInsertIDH2 MTS-cox1 right TALE-G1397 DddA-C-UGI-SV40 polyA
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cox3-FusXTBE-RightTALE
Plasmid#196505PurposeExpresses right-side TALE–DddAtox C term half cox3-FusXTBE in zebrafishDepositorInsertIDH2 MTS-cox3 right TALE-G1397 DddA-C-UGI-SV40 polyA
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
tl1-FusXTBE-RightTALE
Plasmid#196507PurposeExpresses left-side TALE–DddAtox N term half tl1-FusXTBE in zebrafishDepositorInsertIDH2 MTS-tl1 right TALE-G1397 DddA-C-UGI-SV40 polyA
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTWIN1-His6-Ssp-Httex1-7Q
Plasmid#84347PurposeExpression of the human Huntingtin Exon1 protein containing 7Q in E.coliDepositorInsertHuntingtin Exon 1 (HTT Human)
UseTagsN-terminal His6-tagged intein SspExpressionBacterialMutationPromoterT7Available sinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
DSPQ-HTT-TALEN2 BSD
Plasmid#92246PurposeTALEN targeting downstream of HTT gene (ELD) with Blasticidin selection, forms obligate heterodimer with DSPQ-HTT-TALEN1 ZEO. TALEN: NN, HD, NN, NN, HD, NG, NN, NI, NN, NN, HD, NI, NN, HD, NI, NNDepositorInsertTALEN
UseTALENTagsExpressionMutationPromoterCAGAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
DSPQ-HTT-TALEN1 ZEO
Plasmid#92245PurposeTALEN targeting downstream of HTT gene (KKR) with Blasticidin selection, forms obligate heterodimer with DSPQ-HTT-TALEN2 BSD. TALEN: HD, NI, NN, HD, NG, NG, HD, HD, NG, HD, NI, NN, HD, HD, NN, HDDepositorInsertTALEN
UseTALENTagsExpressionMutationPromoterCAGAvailable sinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV2ss-U6-sgHTT1-7sk-sgCas9
Plasmid#190900PurposeAAV-KamiCas9 vector expressing sgGFP and human HTT sgRNAsDepositorInsertsgRNA (HTT Human, spCas9)
UseAAV and CRISPRTagsExpressionMutationPromoterU6 and 7skAvailable sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAVss-U6-sgHTT51-7sk-Cas9
Plasmid#190901PurposeAAV-KamiCas9 vector expressing sgGFP and mouse HTT sgRNADepositorInsertsgRNA (Htt Mouse)
UseAAV and CRISPRTagsExpressionMutationPromoterU6 and 7skAvailable sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTWIN1-His6-Ssp-Httex1-43Q
Plasmid#84352PurposeExpression of the human Huntingtin Exon1 protein containing 43Q in E.coliDepositorInsertHuntingtin Exon 1 (HTT Human)
UseTagsN-terminal Ssp intein (His-tagged)ExpressionBacterialMutationPromoterT7Available sinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTWIN1-His6-Ssp-Httex1-23Q
Plasmid#84349PurposeExpression of the human Huntingtin Exon1 protein containing 23Q in E.coliDepositorInsertHuntingtin Exon 1 (HTT Human)
UseTagsN-terminal Ssp intein (His-tagged)ExpressionBacterialMutationPromoterT7Available sinceJan. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-H1-sgHTT1-U6-sgGFP2-7SK-sgCas9
Plasmid#87917PurposeGateway cloningDepositorInsertsgHTT1, sgGFP2, shCas9
UseTagsExpressionMammalianMutationPromoterH1, U6, 7SKAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTWIN1-His6-Ssp-Httex1-29Q
Plasmid#84350PurposeExpression of the human Huntingtin Exon1 protein containing 29Q in E.coliDepositorInsertHuntingtin Exon 1 (HTT Human)
UseTagsN-terminal Ssp intein (His-tagged)ExpressionBacterialMutationPromoterT7Available sinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTWIN1-His6-Ssp-Httex1-37Q
Plasmid#84351PurposeExpression of the human Huntingtin Exon1 protein containing 37Q in E.coliDepositorInsertHuntingtin Exon 1 (HTT Human)
UseTagsN-terminal Ssp intein (His-tagged)ExpressionBacterialMutationPromoterT7Available sinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTWIN1-His6-Ssp-Httex1-15Q
Plasmid#84348PurposeExpression of the human Huntingtin Exon1 protein containing 15Q in E.coliDepositorInsertHuntingtin Exon1 (HTT Human)
UseTagsN-terminal Ssp intein (His-tagged)ExpressionBacterialMutationPromoterT7Available sinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS416 CUP1pro-Htt103QdeltaP125-134-YFP
Plasmid#224882PurposeLow copy plasmid for inducible yeast expression of the Htt exon 1 construct without the first 10 amino acids of the proline-rich region (125-134) tagged with YFP using copper.DepositorInsertHtt103QdeltaP125-134 (HTT Human)
UseTagsYFPExpressionYeastMutationdeltaP125-134PromoterCUP1Available sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416 CUP1pro-Htt103QdeltaP135-170-YFP
Plasmid#224878PurposeLow copy plasmid for inducible yeast expression of the Htt exon 1 construct without the last 36 amino acids of the proline-rich region (135-170) tagged with YFP using copper.DepositorInsertHtt103QdeltaP135-170 (HTT Human)
UseTagsYFPExpressionYeastMutationdeltaP135-170PromoterCUP1Available sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416 CUP1pro-Htt103QdeltaP141-160-YFP
Plasmid#224886PurposeLow copy plasmid for inducible yeast expression of the Htt exon 1 construct without the middle 20 amino acids of the proline-rich region (141-160) tagged with YFP using copper.DepositorInsertHtt103QdeltaP141-160 (HTT Human)
UseTagsYFPExpressionYeastMutationdeltaP141-160PromoterCUP1Available sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416 CUP1pro-Htt103QdeltaP125-160-YFP
Plasmid#224883PurposeLow copy plasmid for inducible yeast expression of the Htt exon 1 construct without the first 36 amino acids of the proline-rich region (125-160) tagged with YFP using copper.DepositorInsertHtt103QdeltaP125-160 (HTT Human)
UseTagsYFPExpressionYeastMutationdeltaP125-160PromoterCUP1Available sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416 CUP1pro-M-PRR-Htt97QP4-YFP
Plasmid#224885PurposeLow copy plasmid for inducible yeast expression of the Htt exon 1 construct with the last 46 amino acids of the proline-rich region at the N-terminus, 4 prolines left at the C-terminus using copper.DepositorInsertM-PRR-Htt97QP4 (HTT Human)
UseTagsYFPExpressionYeastMutationN-PRRPromoterCUP1Available sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416 CUP1pro-Htt103QdeltaP141-170-YFP
Plasmid#224880PurposeLow copy plasmid for inducible yeast expression of the Htt exon 1 construct without the last 30 amino acids of the proline-rich region (141-170) tagged with YFP using copper.DepositorInsertHtt103QdeltaP141-170 (HTT Human)
UseTagsYFPExpressionYeastMutationdeltaP141-170PromoterCUP1Available sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416 CUP1pro-Htt103QdeltaP161-170-YFP
Plasmid#224876PurposeLow copy plasmid for inducible yeast expression of the Htt exon 1 construct without the last 10 amino acids of the proline-rich region (161-170) tagged with YFP using copper.DepositorInsertHTT103QdeltaP161-170 (HTT Human)
UseTagsYFPExpressionYeastMutationdelta161-170PromoterCUP1Available sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3-luciferase-reporter-HTT-SVA
Plasmid#202833PurposepGL3 luciferase reporter with HTT SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expressionDepositorInsertHTT intronic SVA
UseLuciferaseTagsExpressionMammalianMutationPromoterSV40Available sinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV2ss-EFS-GFP-synPolyA-U6-sgHTT1
Plasmid#190902PurposeAAV vector expressing thew GFP reporter gene and human sgHTTDepositorInsertGFP and sgHTT (HTT Human)
UseAAV and CRISPRTagsExpressionMutationPromoterEFS and U6Available sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Htt171-66Q-myc-WPRE
Plasmid#107934PurposeAdeno-Associated viral (AAV) vector plasmid expressing exon1 of mutant HTT (mHTT, 66 polyQ repeats) in neuronsDepositorInsertExon 1 of mutant HTT, 66 polyQ repeats (HTT Human)
UseAAVTagsExpressionMutationPromoterhSynapsinAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Htt171-18Q-myc-WPRE
Plasmid#107936PurposeAdeno-Associated viral (AAV) vector plasmid expressing exon1 of wild type HTT (wtHTT, 18 polyQ repeats) in neuronsDepositorInsertExon 1 of wild type HTT (HTT Human)
UseAAVTagsExpressionMutationPromoterhSynapsinAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTWIN1-His6-Ssp-Htt2-90-43Q-P90C
Plasmid#114626PurposeBacterial expression of Htt fragment containing amino acids 2-90, with P90C cysteine mutation and a 43 polyQ repeatDepositorInsertHuntingtin Exon1 fragment (HTT Human)
UseTagsHis6-Ssp DnaB inteinExpressionBacterialMutationP90C; 43 polyQ repeatPromoterAvailable sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTWIN1-His6-Ssp-Htt2-90-15Q-P90C
Plasmid#114611PurposeBacterial expression of Htt fragment containing amino acids 2-90, with P90C cysteine mutation and a 15 polyQ repeatDepositorInsertHuntingtin Exon1 fragment (HTT Human)
UseTagsHis6-Ssp DnaB inteinExpressionBacterialMutationP90C; 23 polyQ repeatPromoterAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
SIN-cPPT-H1-sgHTT1-PGK-mCherry-WPRE
Plasmid#87885PurposeTo produce lentiviral vector for HTT editingDepositorInsertshHTT1, cherry
UseLentiviralTagsExpressionMutationPromoterH1, PGKAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q
Plasmid#114617PurposeBacterial expression of Htt fragment containing amino acids 2-90, with A2C cysteine mutation and a 37 polyQ repeatDepositorInsertHuntingtin Exon1 fragment (HTT Human)
UseTagsHis6-Ssp DnaB inteinExpressionBacterialMutationA2C; 23 polyQ repeatPromoterAvailable sinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTWIN1-His6-Ssp-Httex1-43Q-A2C
Plasmid#114622PurposeBacterial expression of Htt fragment containing exon 1, with A2C cysteine mutation and a 43 polyQ repeatDepositorInsertHuntingtin Exon1 (HTT Human)
UseTagsHis6-Ssp DnaB inteinExpressionBacterialMutationA2C; 43 polyQ repeatPromoterAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only