165,567 results

1. pCS2-nCas9n

   Plasmid

   #47929

   Purpose

   expression of an optimized Cas9 for genome-editing in zebrafish

   Depositor

   Wenbiao Chen

   Article

   Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

   Insert

   nls-zcas9-nls
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-Lyso-TORCAR

   Plasmid

   #64929

   Purpose

   Lysosome-targeted FRET biosensor for monitoring mTORC1 kinase activity; targeted to cytosolic face of lysosomal membrane.

   Depositor

   Jin Zhang

   Article

   Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

   Insert

   LAMP1-TORCAR (LAMP1 Human, Synthetic)
   

   Tags

   Cerulean, Full-length LAMP1, and YPet

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. p11d-tRPA(123)

   Plasmid

   #102613

   Purpose

   Expression of human RPA in E. coli

   Depositor

   Marc Wold

   Article

   Henricksen et al J Biol Chem. 1994 Apr 15;269(15):11121-3

   Inserts

   RPA1 (RPA1 Human)
   RPA2 (RPA2 Human)
   RPA3 (RPA3 Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFUGW-Pol2-ffLuc2-eGFP

   Plasmid

   #71394

   Purpose

   Lentiviral vector of luciferase-eGFP fusion gene driven by Pol2 promoter

   Depositor

   Glenn Merlino

   Article

   Day et al Pigment Cell Melanoma Res. 2009 Jun;22(3

   Insert

   Pol2-ffLuc2-eGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   Pol2 (mouse RNA polymerase II gene promtoer)

   Available Since

   Dec. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. lentiCRISPR v2-dCas9

   Plasmid

   #112233

   Purpose

   Lentiviral plasmid for expression of gRNA and dCas9 in mammalian cells; derivative of lentiCRISPR v2

   Depositor

   Thomas Gilmore

   Article

   Babaei et al PLoS One. 2019 Sep 25;14(9):e0222588. do

   Inserts

   dCas9
   Porumycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Changed: Aspartic acid 10 to Glycine, Histidine 8…

   Promoter

   EFS-NS

   Available Since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRDA_174

   Plasmid

   #136476

   Purpose

   Lentiviral expression of EnAsCas12a

   Depositor

   John Doench

   Article

   DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

   Insert

   EnAsCas12a
   

   Use

   CRISPR and Lentiviral

   Promoter

   EF1a

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV mGAD65(delE1) GFP WPRE SV40pA

   Plasmid

   #177316

   Purpose

   To produce the AAV vector that expresses GFP under the control of the mGAD65 promoter. The mGAD65 promoter has highly specificity to GABAergic interneurons.

   Depositor

   Hirokazu Hirai

   Article

   Hoshino et al Mol Brain. 2021 Feb 15;14(1):33. doi: 10

   Insert

   mGAD65(delE1) promoter, GABAergic interneurons specific promoter
   

   Use

   AAV

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBI-CMV1-mir451-CMV2-mir1

   Plasmid

   #164635

   Purpose

   Express miR-451 and miR-1-1 in mammalian cells

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Inserts

   miR451a (MIR451A Human)
   miR-1-1 (MIR1-1 )
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRDA_052

   Plasmid

   #136474

   Purpose

   Lentiviral expression of AsCas12a gRNA

   Depositor

   John Doench

   Article

   DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

   Insert

   Puromycin resistance
   

   Use

   Lentiviral and Synthetic Biology

   Promoter

   EF1a

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCX-TRI-2A

    Plasmid

    #74674

    Purpose

    mammalian expression of TRI-2A (hHO1/F2A/hCD73/F2A/hCD39)

    Depositor

    Roberto Giovannoni

    Article

    Cinti et al PLoS One. 2015 Oct 29;10(10):e0141933. d

    Inserts

    HO1 (HMOX1 Human)
    CD73 (NT5E Human)
    CD39 (ENTPD1 Human)
    

    Tags

    F2A

    Expression

    Mammalian

    Promoter

    pCAGGS

    Available Since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDx_mScarlet-I3

    Plasmid

    #189757

    Purpose

    Dual expression vector for bacteria and mammalian cells producing mScarlet-I3 red fluorescent protein

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet-I3
    

    Tags

    6xHis

    Expression

    Bacterial and Mammalian

    Promoter

    CMV & Rha

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CMV-FLEX-SaCas9-U6-sgSlc32a1

    Plasmid

    #159905

    Purpose

    Mutagenesis of Slc32a1

    Depositor

    Larry Zweifel

    Insert

    Slc32a1 (Slc32a1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA C12 ecDHFR DD YFP HA

    Plasmid

    #192815

    Purpose

    Expresses an N-terminal enhanced destabilizing domain (DD) version of E. coli DHFR with higher basal turnover in mammalian systems. Contains missense mutations W74R/T113S/E120D/Q146L.

    Depositor

    John Hulleman

    Article

    Nakahara et al ACS Chem Biol. 2022 Sep 19. doi: 10.1021

    Insert

    E. coli dihydrofolate reductase
    

    Tags

    enhanced yellow fluorescent protein and hemagglut…

    Expression

    Mammalian

    Mutation

    W74R/T113S/E120D/Q146L

    Promoter

    CMV

    Available Since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSNAP-CaaX Control Plasmid

    Plasmid

    #101133

    Purpose

    Expression of SNAP-Tag and CaaX box (Farnesylation Signal) in Mammalian cells

    Depositor

    New England Biolabs, Ana Egana

    Insert

    CaaX Box
    

    Tags

    SNAP-tag (SNAP26m)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pICH47742:: pRPS5a-PE2max-NC::tHSP

    Plasmid

    #227694

    Purpose

    Golden Gate level 1, position 2, PE2max-NC expression cassette for Arabidopsis.

    Depositor

    Jae-Yean Kim

    Article

    Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

    Insert

    PE2max-NC being driven by pRPS5a and terminated by tHSP
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    pRPS5a

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. Anti-VGAT [L118/80R]

    Plasmid

    #177463

    Purpose

    Mammalian Expression Plasmid of anti-VGAT (Mouse). Derived from hybridoma L118/80.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-VGAT (Mus musculus) recombinant mouse monoclonal antibody (Slc32a1 Mouse)
    

    Expression

    Mammalian

    Promoter

    Dual CMV

    Available Since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. OMMGFP 1-10

    Plasmid

    #164119

    Purpose

    Expresses the non-fluorescent GFP1-10 moiety on the cytosolic face of the OMM

    Depositor

    Tito Calì, Marisa Brini

    Article

    Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

    Insert

    OMMGFP 1-10
    

    Expression

    Mammalian

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. VAMP4-SEP

    Plasmid

    #174406

    Purpose

    Reporter for VAMP4 exocytosis in mammalian cells

    Depositor

    David Perrais

    Article

    Bakr et al Cell Rep. 2021 Sep 7;36(10):109678. doi:

    Insert

    vesicle associated membrane protein 4 (VAMP4 Human)
    

    Tags

    superecliptic phulorin (mutated GFP)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCWX-UBI-hTert-PGK-BSD

    Plasmid

    #114316

    Purpose

    Constitutive expression of the hTert protein

    Depositor

    Patrick Salmon

    Article

    Salmon et al Methods Mol Biol. 2013;945:417-48. doi:

    Insert

    hTERT (TERT Human)
    

    Use

    Lentiviral

    Available Since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. mCherry-CD9-10

    Plasmid

    #55013

    Purpose

    Localization: Tetraspanin/Membrane, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    CD9 (CD9 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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