We narrowed to 4,234 results for: Gca

1. AAV-hSyn1-GCaMP6f-P2A-nls-dTomato

   Plasmid

   #51085

   Purpose

   Neuronal expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore

   Depositor

   Jonathan Ting

   Has Service

   AAV Retrograde and AAV1

   Insert

   GCaMP6f
   

   Use

   AAV

   Tags

   P2A-nls-dTomato

   Expression

   Mammalian

   Mutation

   Promoter

   human Synapsin1

   Available since

   Feb. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE

   Plasmid

   #162381

   Purpose

   AAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expression

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Has Service

   AAV Retrograde, AAV1, AAV5, and AAV9

   Insert

   jGCaMP8m
   

   Use

   AAV and Cre/Lox

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   A25G F286Y

   Promoter

   CAG

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. AAV-CAG-FLEX-NES-jGCaMP8s-WPRE

   Plasmid

   #186046

   Purpose

   AAV-mediated cytosolic expression of jGCaMP8s under the CAG promoter, Cre-dependent expression

   Depositor

   Loren Looger

   Insert

   NES-jGCaMP8s
   

   Use

   AAV

   Tags

   NES-6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. AAV-hSyn1-GCaMP6s-P2A-nls-dTomato

   Plasmid

   #51084

   Purpose

   Neuronal expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophore

   Depositor

   Jonathan Ting

   Has Service

   AAV Retrograde and AAV1

   Insert

   GCaMP6s
   

   Use

   AAV

   Tags

   P2A-nls-dTomato

   Expression

   Mammalian

   Mutation

   Promoter

   human Synapsin1

   Available since

   Feb. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGP-20XUAS-IVS-Syn21-jGCaMP8f-p10

   Plasmid

   #162388

   Purpose

   Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Insert

   jGCaMP8f
   

   Use

   Tags

   6xHis

   Expression

   Insect

   Mutation

   Q315L

   Promoter

   hsp70

   Available since

   May 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AAV-CaMKIIa-GCaMP6s-P2A-nls-dTomato

   Plasmid

   #51086

   Purpose

   Forebrain principle neuron specific expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophore

   Depositor

   Jonathan Ting

   Insert

   GCaMP6s
   

   Use

   AAV

   Tags

   P2A-nls-dTomato

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIa 1.3 kb

   Available since

   March 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AAV-CaMKIIa-GCaMP6f-P2A-nls-dTomato

   Plasmid

   #51087

   Purpose

   Forebrain principle neuron specific expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore

   Depositor

   Jonathan Ting

   Insert

   GCaMP6f
   

   Use

   AAV

   Tags

   P2A-nls-dTomato

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIa 1.3 kb

   Available since

   Feb. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AAV-CAG-FLEX-NES-jGCaMP8m-WPRE

   Plasmid

   #186045

   Purpose

   AAV-mediated cytosolic expression of jGCaMP8m under the CAG promoter, Cre-dependent expression

   Depositor

   Loren Looger

   Insert

   NES-jGCaMP8m
   

   Use

   AAV

   Tags

   NES-6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGP-AAV-syn-FLEX-jGCaMP7f-WPRE

   Plasmid

   #104492

   Purpose

   AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre mediated flip-excision switch

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV1, AAV8, and AAV9

   Insert

   jGCaMP7f
   

   Use

   AAV and Cre/Lox

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-hSynapsin1-axon-jGCaMP8s-P2A-mRuby3

    Plasmid

    #172921

    Purpose

    For enriched expression of jGCaMP8s in axons with cytosolic expression of mRuby3

    Depositor

    GENIE Project

    Has Service

    AAV1 and AAV9

    Insert

    axon-jGCaMP8s-P2A-mRuby3
    

    Use

    AAV

    Tags

    GAP43 palmitoylation domain and P2A-mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin1

    Available since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGP-20XUAS-IVS-Syn21-jGCaMP8m-p10

    Plasmid

    #162387

    Purpose

    Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8m
    

    Use

    Tags

    6xHis

    Expression

    Insect

    Mutation

    A25G F286Y

    Promoter

    hsp70

    Available since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE

    Plasmid

    #104496

    Purpose

    AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switch

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Has Service

    AAV PHP.eB, AAV Retrograde, and AAV1

    Insert

    jGCaMP7f
    

    Use

    AAV and Cre/Lox

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-hSynapsin1-axon-jGCaMP8f-P2A-mRuby3

    Plasmid

    #172923

    Purpose

    For enriched expression of jGCaMP8f in axons with cytosolic expression of mRuby3

    Depositor

    GENIE Project

    Has Service

    AAV1 and AAV9

    Insert

    axon-jGCaMP8f-P2A-mRuby3
    

    Use

    AAV

    Tags

    GAP43 palmitoylation domain (N terminal on insert…

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin1

    Available since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-Ef1a-Con/Foff 2.0-GCaMP6M

    Plasmid

    #137120

    Purpose

    Intersectional viral expression of GCaMP6M in cells expressing Cre AND NOT Flp

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Foff 2.0-GCaMP6M
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    Removed RSET tag

    Promoter

    EF1a

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGP-AAV-syn-FLEX-jGCaMP7b-WPRE

    Plasmid

    #104493

    Purpose

    AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre mediated flip-excision switch

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Has Service

    AAV Retrograde and AAV1

    Insert

    jGCaMP7b
    

    Use

    AAV and Cre/Lox

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGP-AAV-CAG-FLEX-jGCaMP7b-WPRE

    Plasmid

    #104497

    Purpose

    AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switch

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Insert

    jGCaMP7b
    

    Use

    AAV and Cre/Lox

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-EF1a-FLEX(frt)-GCaMP6f-WPRE

    Plasmid

    #118273

    Purpose

    pAAV vector for flippase dependent GCaMP6f expression under the control of EF1a promoter

    Depositor

    Naoshige Uchida

    Article

    Menegas et al Nat Neurosci. 2018 Sep 3. pii: 10.1038/s

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    6xHis (N terminal on insert), T7 epitope, and Xpr…

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mCherry-NES-SspB-C3Gcat-P2A-iLIDcaax

    Plasmid

    #173874

    Purpose

    Mammalian expression of mVenus-NES-SspB-C3Gcat-P2A-iLIDcaax (opto-Rap)

    Depositor

    Takao Nakata

    Article

    Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

    Insert

    mCherry-NES-SspB-C3Gcat-P2A-iLIDcaax
    

    Use

    Tags

    C-terminal CAAX-box and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-CAG-DIO-CFL-SN-P2A-GCaMP6f

    Plasmid

    #181742

    Purpose

    AAV vector for expressing CFL-SN and GCaMP6f

    Depositor

    Yasunori Hayashi

    Article

    Goto et al Science. 2021 Nov 12;374(6569):857-863.

    Insert

    CFL-SN-P2A-GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAV-CAG-FLEX-NES-jGCaMP8f-WPRE

    Plasmid

    #186044

    Purpose

    AAV-mediated cytosolic expression of jGCaMP8f under the CAG promoter, Cre-dependent expression

    Depositor

    Loren Looger

    Insert

    NES-jGCaMP8f
    

    Use

    AAV

    Tags

    NES-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. mVenus-NES-SspB-C3Gcat-P2A-iLIDcaax

    Plasmid

    #173868

    Purpose

    Mammalian expression of mVenus-NES-SspB-C3Gcat-P2A-iLIDcaax (opto-Rap)

    Depositor

    Takao Nakata

    Article

    Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

    Insert

    mVenus-NES-SspB-C3Gcat-P2A-iLIDcaax
    

    Use

    Tags

    C-terminal CAAX-box and mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. VV209: sdChR(C138S E154A)-TS-GCaMP6f-ER in fck

    Plasmid

    #58524

    Purpose

    Expresses sdChR(C138S E154A) fused to GCaMP6f in mammalian cells

    Depositor

    Adam Cohen

    Article

    Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

    Insert

    sdChR(C138S, E154A)-TS-GCaMP6f-ER
    

    Use

    Lentiviral

    Tags

    GCaMP6f

    Expression

    Mammalian

    Mutation

    changed Cysteine 138 to Serine; changed Glutamate…

    Promoter

    a-CamKII

    Available since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-Ef1a-Con/Fon/Von GCaMP 6m

    Plasmid

    #137165

    Purpose

    Intersectional viral expression of GCaMP6M in cells expressing Cre AND Flp AND Vcre

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Insert

    3x-GCaMP6M
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    Removed RSET tag

    Promoter

    Ef1a

    Available since

    Nov. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. AAV Cag Flex H2B Gcamp6s reverse

    Plasmid

    #74157

    Purpose

    Calcium Sensor

    Depositor

    Loren Looger

    Insert

    Gcamp6s
    

    Use

    AAV

    Tags

    H2B

    Expression

    Mutation

    Promoter

    SpeI

    Available since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. AAV-CAG-FLEX-LifeAct-jGCaMP8f-WPRE

    Plasmid

    #186047

    Purpose

    AAV-mediated expression of LifeAct-jGCaMP8f under the CAG promoter, Cre-dependent expression

    Depositor

    Loren Looger

    Insert

    LifeAct-jGCaMP8f
    

    Use

    AAV

    Tags

    LifeAct-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. AAV-CAG-FLEX-LifeAct-jGCaMP8m-WPRE

    Plasmid

    #186048

    Purpose

    AAV-mediated expression of LifeAct-jGCaMP8m under the CAG promoter, Cre-dependent expression

    Depositor

    Loren Looger

    Insert

    LifeAct-jGCaMP8m
    

    Use

    AAV

    Tags

    LifeAct-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pOTTC813 - pAAV-SYN1-CRTsigpep-GCaMP3-KDEL

    Plasmid

    #63886

    Purpose

    An AAV packaging vector that expresses ER-retained GCaMP3 under control of the SYN1 promoter.

    Depositor

    Brandon Harvey

    Article

    Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

    Insert

    ER-localized GCaMP3(wt)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    human SYN1

    Available since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pOTTC809 - pAAV-EF1a-CRTsigpep-GCaMP3-KDEL

    Plasmid

    #63884

    Purpose

    An AAV packaging vector that expresses ER-retained GCaMP3 under control of the EF1a promoter.

    Depositor

    Brandon Harvey

    Article

    Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

    Insert

    ER-localized GCaMP3(wt)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. AAV-EF1α-loxP-GCaMP6f-loxP-WPRE

    Plasmid

    #233288

    Purpose

    AAV vector with EF1α promoter and Cre-Out GCaMP6f, enabling to express GCaMP6f in Cre-negative cells.

    Depositor

    Dayu Lin

    Article

    Fang et al Neuron. 2018 Apr 4;98(1):192-207.e10. do

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1α

    Available since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pX552-hsyn DIO GCaMP8f(with gRNA scaffold)

    Plasmid

    #199580

    Purpose

    Expresses GCaMP8f in a Cre-dependent manner

    Depositor

    Bryan Copits

    Article

    Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

    Insert

    His-DIO GCaMP8f
    

    Use

    AAV, CRISPR, and Cre/Lox

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    hsyn

    Available since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAAV2ss-EFS-GFP-synPolyA-U6-sgHTT1-U6-sgCas9

    Plasmid

    #190903

    Purpose

    AAV-KamiCas9 vector expressing expressing thew GFP reporter gene and sgHTT and sgCas9

    Depositor

    Nicole Déglon

    Article

    Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

    Insert

    GFP, sgHTT and sgCas9 (HTT Human)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    EFS and U6

    Available since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. AAV-CAG-FLEX-LifeAct-jGCaMP8s-WPRE

    Plasmid

    #186049

    Purpose

    AAV-mediated expression of LifeAct-jGCaMP8s under the CAG promoter, Cre-dependent expression

    Depositor

    Loren Looger

    Insert

    LifeAct-jGCaMP8s
    

    Use

    AAV

    Tags

    LifeAct-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE

    Plasmid

    #131004

    Purpose

    bicistronic AAV vector to express GCaMP6m and soma-targeted ChRmine under the control of human Synapsin promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Has Service

    AAV8

    Insert

    ChRmine
    

    Use

    AAV

    Tags

    Kv2.1-HA

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato

    Plasmid

    #51083

    Purpose

    Cre dependent expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore

    Depositor

    Jonathan Ting

    Has Service

    AAV Retrograde

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    P2A-nls-dTomato

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1a

    Available since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAV-hSynapsin1-FLEx-axon-jGCaMP8m-WPRE-SV40

    Plasmid

    #216533

    Purpose

    An axon-targeted jGCaMP8m controlled by hSynapsin1 promoter and flex switch

    Depositor

    Yuki Kambe

    Article

    Abe et al bioRxiv. 2023 Aug 24:2023.08.23.554475.

    Insert

    axon-jGCaMP8m
    

    Use

    AAV and Cre/Lox

    Tags

    GAP43

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin1

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAAV-CaMKIIa-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE

    Plasmid

    #131003

    Purpose

    bicistronic AAV vector to express GCaMP6m and soma-targeted ChRmine under the control of CamKIIa promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Insert

    ChRmine
    

    Use

    AAV

    Tags

    Kv2.1-HA

    Expression

    Mutation

    Promoter

    CaMKIIa

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBAC-ie1-DsRed-ObirOrco-QF2-15xQUAS-GCaMP6s

    Plasmid

    #200400

    Purpose

    Expresses DsRed broadly, expresses QF2 and GCaMP6s in OSNs of the clonal raider ant

    Depositor

    Daniel Kronauer

    Article

    Hart et al Cell. 2023 Jul 6;186(14):3079-3094.e17.

    Inserts

    Discosoma red fluorescent protein
    Q factor 2
    GCaMP6s
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    15x QUAS, ObirOrco (Oocearaea biroi Orco promoter…

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAAV-hSyn-DIO {hCAR}off-{GCaMP6f}on-W3SL

    Plasmid

    #111394

    Purpose

    AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON GCaMP6f (ultrasensitive calcium sensor), and W3SL regulatory cassette (for maximize cloning capacity)

    Depositor

    Adam Kepecs

    Article

    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

    Inserts

    hCAR (CXADR Human)
    GCaMP6f
    

    Use

    AAV

    Tags

    6xHis, Myc, T7, and Xpress

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV-hSyn-DIO {mCAR}off-{GCaMP6f}on-WPRE

    Plasmid

    #111393

    Purpose

    AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection) and Cre-ON GCaMP6f (ultrasensitive calcium sensor)

    Depositor

    Adam Kepecs

    Article

    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

    Inserts

    mCAR (Cxadr Mouse)
    GCaMP6f
    

    Use

    AAV

    Tags

    6xHis, Myc, T7, and Xpress

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAAV-hSynapsin1-FLEx-axon-GCaMP6s-P2A-mRuby3

    Plasmid

    #112008

    Purpose

    For cre-dependent enriched expression of GCaMP6s in axons with cytosolic expression of mRuby3

    Depositor

    Lin Tian

    Article

    Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

    Insert

    axon-GCaMP6s-P2A-mRuby3
    

    Use

    AAV and Cre/Lox

    Tags

    GAP43 palmitoylation domain

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin1

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pGP-AAV-syn-jGCaMP7c variant 1513-WPRE

    Plasmid

    #105321

    Purpose

    AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Has Service

    AAV1

    Insert

    jGCaMP7c variant 1513
    

    Use

    AAV

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    Jan. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato

    Plasmid

    #51082

    Purpose

    Cre dependent expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophore

    Depositor

    Jonathan Ting

    Has Service

    AAV1

    Insert

    GCaMP6s
    

    Use

    AAV

    Tags

    P2A-nls-dTomato

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1a

    Available since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8s-p10

    Plasmid

    #162383

    Purpose

    LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8s
    

    Use

    Tags

    6xHis

    Expression

    Insect

    Mutation

    S26M F286Y Q315H

    Promoter

    hsp70

    Available since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8m-p10

    Plasmid

    #218862

    Purpose

    Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

    Insert

    RSET-jGCaMP8m
    

    Use

    Tags

    6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

    Expression

    Insect

    Mutation

    A25G F286Y

    Promoter

    hsp70

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA

    Plasmid

    #50942

    Purpose

    Bicistronic vector expressing mRuby2 and GCaMP6s from a single open reading frame.

    Depositor

    Mark Huebener, Tobias Rose, Tobias Bonhoeffer

    Article

    Rose et al Science. 2016 Jun 10;352(6291):1319-22.

    Has Service

    AAV1

    Insert

    mRuby2-P2A-GCaMP6s
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn1

    Available since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA

    Plasmid

    #50943

    Purpose

    Bicistronic vector expressing mRuby2 and GCaMP6f from a single open reading frame.

    Depositor

    Mark Huebener, Tobias Rose, Tobias Bonhoeffer

    Article

    Rose et al Science. 2016 Jun 10;352(6291):1319-22.

    Insert

    mRuby2-P2A-GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn1

    Available since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8f-p10

    Plasmid

    #218866

    Purpose

    Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

    Insert

    RSET-jGCaMP8f
    

    Use

    Tags

    6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

    Expression

    Insect

    Mutation

    Q315L

    Promoter

    hsp70

    Available since

    May 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8f-p10

    Plasmid

    #162385

    Purpose

    LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8f
    

    Use

    Tags

    6xHis

    Expression

    Insect

    Mutation

    Q315L

    Promoter

    hsp70

    Available since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8m-p10

    Plasmid

    #162384

    Purpose

    LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8m
    

    Use

    Tags

    6xHis

    Expression

    Insect

    Mutation

    A25G F286Y

    Promoter

    hsp70

    Available since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pMOS008E: Entry vector for: GCaMP6F calcium sensor (cytosolic)

    Plasmid

    #163064

    Purpose

    Entry vector for: GCaMP6F calcium sensor (cytosolic)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    GCaMP6F
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge