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Plasmid#52014PurposeUsed for in vitro expression of the human MAVS CDS containing a point mutation G67MDepositorInsertMAVS
UseTagsExpressionMammalianMutationChanged Gly 67 to MetPromoterAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-MAVS-L62M-invitro(KaganD98)
Plasmid#52013PurposeUsed for in vitro expression of the human MAVS CDS containing a point mutation L62MDepositorInsertMAVS
UseTagsExpressionMammalianMutationChanged Leu 62 to MetPromoterAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-MAVS-E80M-invitro(KaganD99)
Plasmid#52015PurposeUsed for in vitro expression of the human MAVS CDS containing a point mutation E80MDepositorInsertMAVS
UseTagsExpressionMammalianMutationChanged Glu 80 to MetPromoterAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-MAVS-M142A-invitro(E13)
Plasmid#52017PurposeUsed for in vitro expression of the human MAVS CDS containing a point mutation M142ADepositorInsertMAVS-M142A
UseTagsExpressionMammalianMutationchanged Met 142 to AlaPromoterAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJUMP18-B0032-MV_RN
Plasmid#127015PurposeBasic Part RN- RBS+N-terminus; B0033m Ribosome Binding Site + Met-Val N-terminus start.DepositorInsertPart B0032m-MV_RN
UseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT IAA17
Plasmid#193319PurposeC-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin-Inducible Degron). ARF16(PB1)-P2A-osTIR1 has SIR4 homology arms for genome integration.DepositorInsertIAA17
UseTagsIAA17ExpressionYeastMutationPromoterAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT Compatible pAIDA2(6FLAG)
Plasmid#193321PurposeC-SWAT compatible vector for C-terminal tagging with TAP-AID*-6FLAG. osTIR1 is carried downstream of the C-terminal tag.DepositorInsertTAP-AID*-6FLAG
UseTagsTAP-AID*-6FLAGExpressionYeastMutationPromoterAvailable sinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT AID*-6MYC osTIR1
Plasmid#193320PurposeC-SWAT compatible vector for C-terminal tagging with AID*-6MYC for auxin induced gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration into the genome.DepositorInsertAID*
UseTags6MYCExpressionYeastMutationPromoterAvailable sinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT AID*-6FLAG osTIR1
Plasmid#182515PurposeC-SWAT compatible vector for C-terminal tagging with AID*-6FLAG for auxin induced gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration into the genome.DepositorInsertAID*
UseTags6FLAGExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT IAA17-6FLAG
Plasmid#182516PurposeC-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin-Inducible Degron) and 6FLAG. ARF16(PB1)-P2A-osTIR1 has SIR4 homology arms for genome integration.DepositorInsertIAA17
UseTags6FLAGExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT IAA17-3HA
Plasmid#182517PurposeC-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin inducible degron) and 3HA. ARF16(PB1)-P2A-osTIR1 with SIR4 homology arms for genome integration.DepositorInsertIAA17
UseTags3HAExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT IAA17-3MYC
Plasmid#182518PurposeC-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin inducible degron) and 3MYC. ARF16(PB1)-P2A-osTIR1 with SIR4 homology arms for genome integration.DepositorInsertIAA17
UseTags3MYCExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT IAA17-6FLAG
Plasmid#182522PurposeC-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin Inducible Degron) and 6FLAG. ARF16(PB1)-P2A-osTIR1 with CAN1 homology arms for genome integration.DepositorInsertIAA17
UseTags6FLAGExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJUMP18-B0033-MV_RN
Plasmid#127016PurposeBasic Part RN- RBS+N-terminus; B0034m Ribosome Binding Site + Met-Val N-terminus start.DepositorInsertPart B0033m-MV_RN
UseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-MAVS-M1A(KaganE18)
Plasmid#52009PurposeExpresses the human MAVS CDS containing the point mutation M1ADepositorInsertMAVS-M1A
UseRetroviralTagsExpressionMammalianMutationchanged Met 1 to AlaPromoterAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-MAVS-M142A(KaganE19)
Plasmid#52010PurposeExpresses the human MAVS CDS containing the point mutation M142ADepositorInsertMAVS-M142A
UseRetroviralTagsExpressionMammalianMutationchanged Met 142 to AlaPromoterAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-MAVS-M142A(KaganF59)
Plasmid#52062PurposeExpresses the human MAVS CDS containing the point mutation M142A "weak translational context"DepositorInsertMAVS-M142A
UseTagsExpressionMammalianMutationchanged Met 142 to AlaPromoterAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a hMDH2
Plasmid#204230PurposeBacterial expression of mitochondrial human malate dehydrogenase 2 (hMDH2) transcript variant 1; Use in CURES (integration of research into undergraduate teaching labs).InserthMDH2 (MDH2 Human)
UseTags6X HisExpressionBacterialMutationDoes not include transit peptide and includes a M…PromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pYES2.1-pomSir1
Plasmid#107447PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertSir1 (sir1 Fission Yeast)
UseTagsV5-HisExpressionYeastMutationH523R, A750TPromoterGAL1Available sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
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C-SWAT IAA17-6FLAG (no NLS)
Plasmid#193322PurposeC-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin-Inducible Degron) and 6FLAG. ARF16(PB1)-P2A-osTIR1 has SIR4 homology arms for genome integration and does not have NLS tags..DepositorInsertIAA17
UseTags6FLAGExpressionYeastMutationPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT GFP CL1 DAmP
Plasmid#182513PurposeC-SWAT compatible vector for C-terminal tagging with GFP CL1 followed by the DAmP cassette. The stop codon after the CL1 degron is immediately followed by the Hygromycin B (Hph) resistance cassette.DepositorInsertCL1 and DAmP
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJUMP18-RBS-pET-MV_RN
Plasmid#127014PurposeBasic Part RN- RBS+N-terminus; B0032m Ribosome Binding Site + Met-Val N-terminus start.DepositorInsertPart RBS-pET-MV_RN
UseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-MAVS-M1A500(KaganF60)
Plasmid#52003PurposeExpresses the human MAVS CDS containing a point mutation M1A and a C-terminal deletion removing the transmembrane domainDepositorInsertMAVS-M1A-500
UseTagsExpressionMammalianMutationchanged Met 1 to Ala and deleted the C-terminal t…PromoterAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVP91A-pcaHG
Plasmid#113766PurposeExpresses the alpha (pcaG) and the beta (pcaH) subunits of Protocatechuate 3,4-Dioxygenase from Pseudomonas putida.DepositorInsertsβ subunit of Protocatechuate 3,4- Dioxygenase
α subunit of Protocatechuate 3,4- Dioxygenase
UseTagsMet-6xHisExpressionBacterialMutationPromoterT5 promoterAvailable sinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
TrpLE-M-nMSP1E3D1
Plasmid#179949PurposeTagless membrane scaffold protein (MSP) for formation of nanodiscs.DepositorInsertTrpLE-M-nMSP1E3D1
UseTagsTrpLEExpressionBacterialMutationNative Trp residues have been mutated to Phe resi…PromoterT7Available sinceFeb. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET11a-4SS-HEWL
Plasmid#39233DepositorInsertHen egg white lysozyme (LYZ Chicken)
UseTagsExpressionBacterialMutationN-terminal MetPromoterT7Available sinceSept. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
SG3ΔENV CA M66I
Plasmid#149687PurposeNon-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and M66I mutation in capsid (CA M66I).DepositorInsertCapsid
UseLentiviral and RetroviralTagsExpressionMammalianMutationChanged Capsid residue Met 66 to Iso (CA M66I)PromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET23-PBD(65-150)-N-Cys
Plasmid#13722DepositorInsertPBD(65-150)-N-Cys (PAK1 Human)
UseTags6xHisExpressionBacterialMutationMet-Cys-Gly-PBD(65-150)-PromoterAvailable sinceMarch 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGCNK58
Plasmid#202461PurposePlasmid for the expression of the basic region and leucine zipper domain of the Saccharomyces cerevisiae transcription factor GCN4 in bacterial cellsDepositorInsertResidues 226-281 of Sacchromyces cerevisiae GCN4 plus an N-terminal Met-Lys extension (GCN4 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pTriEx4-H6-FGAm (FlincG3)
Plasmid#49202PurposeFluorescent reporter for cGMPDepositorInsertFlincG3
UseTagsHis tag + other tagsExpressionBacterial, Insect, and Mamm…MutationMet to Lys in cpEGFP region similar to CGaMP3PromoterCMVAvailable sinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_LAMA-F98 (for GFP)
Plasmid#130714PurposePlasmid encoding the bacterial expression of LAMA-F98 for GFPDepositorInsertLAMA-F98
UseTagsHisx10ExpressionBacterialMutationDeleted first Met, Ala and Last Ser from the Enha…PromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV/N3IC-HA
Plasmid#47618Purposein vitro transcription and translation of HA-tagged Notch3 Intracellular domainDepositorInsertNotch3 Intracellular domain (Notch3 Mouse)
UseIn vitro transcription and translationTagsHAExpressionMammalianMutationcodons 1664 (Met) to 2318 (Ala)PromoterCMVAvailable sinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTrc99a-NoSS-TolC
Plasmid#155179PurposeExpresses signal sequence-less TolC as inclusion bodiesDepositorInsertTolC (tolC )
UseTagsExpressionBacterialMutationdeleted residues 1-20, residue 21 mutated to Met …PromotertrcAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_SNAP-p24
Plasmid#130718PurposePlasmid encoding the bacterial expression of a SNAP-fusion of p24 HIV Capsid proteinDepositorInsertSNAP-Tag-p24
UseTagsHisx10 and StrepTagExpressionBacterialMutationDeleted first Met, Ala and Last Ser from the Enha…PromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_LAMA-G97 (for GFP)
Plasmid#130715PurposePlasmid encoding the bacterial expression of LAMA-G97 for GFPDepositorInsertLAMA-G97
UseTagsHisx10ExpressionBacterialMutationDeleted first Met, Ala and Last Ser from the Enha…PromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_LAMA-N95 (for GFP)
Plasmid#130716PurposePlasmid encoding the bacterial expression of LAMA-N95 for GFPDepositorInsertLAMA-N95
UseTagsHisx10ExpressionBacterialMutationDeleted first Met, Ala and Last Ser from the Enha…PromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
ETV3_pet28a
Plasmid#131646PurposeBacterial expression of ETS gene ETV3DepositorInsertETV3 (ETV3 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
peT3a-AqAdk_MVGDH
Plasmid#18092DepositorInsertadenylate kinase (kad A. aeolicus)
UseTags6xHisExpressionBacterialMutationTyr 52 changed to Cys, Val 145 changed to Cys, …PromoterAvailable sinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-null (BPK1077)
Plasmid#171128PurposePlasmid encoding a pCMV-T7 with a 2 amino acid peptide prior to a stop codon.DepositorInsertpCMV-T7 null expression plasmid that encodes a two amino acid peptide (Met-Asp)
UseCRISPRTagsExpressionBacterial and MammalianMutationPromoterpCMV and T7Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
3XFlagNICD2
Plasmid#20184DepositorInsertNotch 2 intracellular domain (Notch2 Mouse)
UseTags3X FLAGExpressionMammalianMutationContains murine Notch 2 Intracellular Domain (Met…PromoterCMVAvailable sinceJan. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
3XFlagNICD3
Plasmid#20185DepositorInsertNotch 3 Intracellular Domain (Notch3 Mouse)
UseTags3X FlagExpressionMammalianMutationContains murine Notch 3 Intracellular Domain (Met…PromoterCMVAvailable sinceDec. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
AAV-RO1.7-RPE65
Plasmid#206346PurposeAAV plasmid expressing RPE65 in photoreceptorsDepositorInsertRpe65 (Rpe65 Mouse)
UseAAVTagsExpressionMutation450-Met (C57BL6 natural variation)Promoter1.7kb human red opsinAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
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YFP(1-158) in pcDNAI/Amp
Plasmid#60888PurposeThis vector attaches YFP(1-158) to the N-terminus of a protein.DepositorInsertYFP(1-158)
UseTagsExpressionMammalianMutationMet was substituted for Gln-69.PromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -