Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 8,244 results for: 11

Clear keywords
Showing: 181 - 200 of 8244 results

  3. pcDNA3-10His-mStayGold (E138D)

    Plasmid
    #211363
    Purpose
    Mammalian expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Contains a N-terminal 10xHis tag.
    Depositor
    Mohan Balasubramanian
    Publication
    Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mStayGold
    Tags
    10xHis-tag
    Expression
    Mammalian
    Mutation
    E138D
    Promoter
    CMV
    Available since
    Dec. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    211363-plasmid-map-sequence-id-417674

    Enlarge

  5. Puro-Cas9 donor

    Plasmid
    #58409
    Purpose
    Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus
    Depositor
    Danwei Huangfu
    Publication
    Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hSpCas9
    Use
    CRISPR
    Tags
    3xFlag, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    18 Articles
    58409-plasmid-map-sequence-id-392399

    Enlarge

  9. pcDNA3-Lyso-TORCAR

    Plasmid
    #64929
    Purpose
    Lysosome-targeted FRET biosensor for monitoring mTORC1 kinase activity; targeted to cytosolic face of lysosomal membrane.
    Depositor
    Jin Zhang
    Publication
    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LAMP1-TORCAR (LAMP1 Human, Synthetic)
    Tags
    Cerulean, Full-length LAMP1, and YPet
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    64929-plasmid-map-sequence-id-490376

    Enlarge

  16. pdCas9-DNMT3A-PuroR

    Plasmid
    #71667
    Purpose
    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Publication
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A S. pyogenes, Human, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    CBh
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    8 Articles
    71667-plasmid-map-sequence-id-243369

    Enlarge

  17. pAAV/D374Y-hPCSK9

    Plasmid
    #58379
    Purpose
    Expresses GoF mutant human PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosis
    Depositor
    Jacob Bentzon
    Publication
    Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. do
    Has service
    AAV8
    Insert
    proprotein convertase subtilisin/kexin type 9 (PCSK9 Human)
    Use
    AAV
    Expression
    Mammalian
    Mutation
    D374Y
    Promoter
    HCRApoE/hAAT
    Available since
    Aug. 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    58379-plasmid-map-sequence-id-333468

    Enlarge

Showing: 181 - 200 of 8244 results