We narrowed to 9,280 results for: Pol;
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Plasmid#59781PurposeExpress H3.3 chaperone HIRA in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.DepositorInsertHIRA
ExpressionMammalianPromoterCMV and T7Available SinceSept. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
POLR2A-N CRISPR pX330
Plasmid#124495PurposeA CRISPR plasmid for targeting the N-terminus coding region of human POLR2ADepositorInsertPOLR2A targeting CRISPR
UseCRISPRPromoterU6 promoterAvailable SinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_WT POLI
Plasmid#131228PurposeExpression of WT DNA polymerase iota with an N-terminal FLAG tag in mammalian cellsDepositorInsertDNA polymerase iota (POLI Human)
TagsFLAGExpressionMammalianMutationCoding sequence has been codon optimised for expr…PromoterCMV enhancer + CMV promoterAvailable SinceDec. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
mDlx-BiPOLES-mCerulean
Plasmid#154946PurposeOptogenetic tool for blue-light inhibition and red-light excitation of neuronsDepositorInsertBiPOLES
UseAAVExpressionMammalianPromoterminimal DlxAvailable SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOT2-poly-cis
Plasmid#141177PurposeTo clone any gene or STTM sequence or artificial miRNA under CaMV 35S promoterDepositorTypeEmpty backboneUseRNAi and Synthetic BiologyExpressionPlantPromoterCaMV 35S promoterAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVENUS-N1-POLR2G-GFP
Plasmid#86872PurposeTo express POLR2G (RPB7) tagged with VENUS at C terminus.DepositorAvailable SinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
HO-pMET-poly-KanMX4-HO
Plasmid#51665Purposeyeast plasmid for integration of target sequence at HO locus, Kan selection, pMET inducible promoterDepositorTypeEmpty backboneUseYeast integrationExpressionYeastAvailable SinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFIV-H1-Puro-hPolß
Plasmid#18663DepositorInsertshRNA specific to human DNA polymerase beta (POLB Human)
UseLentiviral and RNAiExpressionMammalianMutationnoneAvailable SinceOct. 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
attB-mCh-Poly(A)
Plasmid#65525PurposePlasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.DepositorInsertattB-mCherry
UseMouse Targeting; Bxb1ExpressionMammalianAvailable SinceAug. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_UBM1*2* POLI
Plasmid#131232PurposeMammalian expression of an N-terminally FLAG-tagged DNA polymerase iota mutant, containing point mutations in both UBM domainsDepositorInsertDNA polymerase iota (POLI Human)
TagsFLAGExpressionMammalianMutationContains inactivating mutations in both ubiquitin…PromoterCMV enhancer + CMV promoterAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
attB-GFP-Poly(A)
Plasmid#65524PurposePlasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.DepositorInsertattB-GFP
UseMouse Targeting; Bxb1ExpressionMammalianAvailable SinceAug. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyA
Plasmid#97308PurposeHMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.DepositorInsertActb HMEJ donor
UseAAV and Mouse TargetingExpressionMammalianPromoterU6, EF1aAvailable SinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-GST-PolB(WT)
Plasmid#177131PurposeBacterial vector for expression of an N-terminal GST fusion of PolB(WT) with a TEV protease site located between the GST tag and PolB(WT)DepositorAvailable SinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
gRNA-POLQ-Halo
Plasmid#211636PurposesgRNA against POLQDepositorInsertsgRNA POLQ
UseCRISPRExpressionMammalianPromoterU6Available SinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV_Actb MMEJ donor_U6_sgRNA_EF1a_GFP_polyA
Plasmid#97311PurposeMMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.DepositorInsertActb MMEJ donor
UseAAV and Mouse TargetingExpressionMammalianPromoterU6, EF1aAvailable SinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pESC-URA-hPOLISc
Plasmid#60819Purposeto express human Pol iota recoded to yeast codons in yeastDepositorInsertPOLI (POLI Human)
Tagsc-mycExpressionYeastMutationoptimized to yeast codon usagePromoterGAL1Available SinceMarch 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 Cas9-POLD3
Plasmid#183197PurposeEntry cloneDepositorInsertCas9-POLD3
UseCRISPR; Gateway entry cloneMutationn/aAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyA
Plasmid#97312PurposeHMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. Lenti backbone.DepositorInsertActb HMEJ donor
UseLentiviral and Mouse TargetingExpressionMammalianPromoterU6, EF1aAvailable SinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
FRB-polySH33-EGFP
Plasmid#175792PurposeBacterial expression and purification, fluorescently tagged scaffold for droplet visualization and volume quantificationDepositorAvailable SinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBSc polyA + Shal1
Plasmid#16196DepositorInsertDrosophila melanogaster Shaker cognate l (Shal Fly)
UseXenopus oocyte expressionMutationN/AAvailable SinceJan. 10, 2008AvailabilityAcademic Institutions and Nonprofits only