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Plasmid#193644PurposeExpression of base editor dCas12a-ABE7 in mammalian cells for base editing, along with non-fused EGFP fluorescenceDepositorInsertdLbCas12a-TadA-TadA*7.10, EGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNHEJ-Cas12a-recX
Plasmid#158714PurposeCRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses FnCpf1, NHEJ machinery of M. marinum and recXDepositorInsertrecX
UseCRISPRTagsExpressionBacterialMutationPromoterPmycAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJCC_076 AsCas12a R1226A
Plasmid#171669PurposeFor bacterial expression of AsCas12a R1226A (RuvC-inhibiting mutation) with an N-terminal His-MBP tagDepositorInsertAsCas12a R1226A
UseTags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged arginine 1226 to alaninePromoterAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
AsCas12a-2C-NLS in pCSDest
Plasmid#126637PurposeExpresses AsCas12a-2C-NLS in mammalian cellsDepositorInsertAsCas12a-2C-NLS
UseCRISPRTags3xHuman influenza hemagglutinin (HA)-TagExpressionMammalianMutationPromoterCMV IE94 promoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
plenti-As-Cas12a-2xNLS
Plasmid#155047PurposeLentiviral vector expressing human codon-optimized _C-terminal Myc-tagged _Acidaminococcus sp. BV3L6 (As)-Cas12a nuclease with N- and C-terminal NLS and Neomycin/G418/Geneticin resistance from EF-1alpha promoterDepositorInsertAsCas12a
UseCRISPR and LentiviralTagsNucleoplasmin NLS, Myc-tag and SV40 NLSExpressionMammalianMutationPromoterEF-1aAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCJH009_proD_Cas12c-sgRNA-GFP_CAM
Plasmid#183070PurposePlasmid expressing Cas12c sgRNA targeting GFP (1) for in vivo interference assay in bacteria.DepositorInsertCas12c single guide RNA targeting GFP
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-AsCas12a-2AeGFP
Plasmid#123638PurposeMammalian expression, Genome editingDepositorInsertAsCas12a
UseTags3xHA tagExpressionMammalianMutationPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-AaCas12b-2AeGFP
Plasmid#121945PurposeMammalian expression, Genome editingDepositorInsertAaCas12b
UseTags3xHA tagExpressionMammalianMutationPromoterAvailable sinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMBP-LbCas12a D832A
Plasmid#113517PurposeExpresses His-MBP fused to LbCas12a with D832A mutation to inactivate RuvC nucleaseDepositorInsertLbCas12a
UseTagsMBPExpressionBacterialMutationD832APromoterT7Available sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZ143-pcDNA3-BvCas12b
Plasmid#122445PurposeExpresses BvCas12b in mammalian cellsDepositorInsertCMV-NLS-BvCas12b-NLS-3xHA
UseTags3XHAExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV-AsCas12f-v4.1
Plasmid#204636PurposeExpression of AsCas12f v4.1 in mammalian cellsDepositorInsertAsCas12f-v4.1
UseTagsNLSExpressionMammalianMutationD169K, N199K, N328G, D364RPromoterCMVAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-PxCas12a-2AeGFP
Plasmid#123637PurposeMammalian expression, Genome editingDepositorInsertPxCas12a
UseTags3xHA tagExpressionMammalianMutationPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUbi-RZ-Cas12j2
Plasmid#173926PurposeGateway entry clone (attL5 & attL2) for Cas12j2 crRNA expression under ZmUbi promoter with ribozyme processingDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKEW107-MbCas12a-33362
Plasmid#115654PurposeExpresses MbCas12a in bacteria from M. bovoculi strain 33362, under TetR controlDepositorInsertMbCas12a
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterPtetAvailable sinceOct. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKEW106-MbCas12a-58069
Plasmid#115653PurposeExpresses MbCas12a in bacteria from M. bovoculi strain 58069, under TetR controlDepositorInsertMbCas12a
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterPtetAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-AkCas12b-2AeGFP
Plasmid#121946PurposeMammalian expression, Genome editingDepositorInsertAkCas12b
UseTags3xHA tagExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJCC_075 AsCas12a D908A
Plasmid#171668PurposeFor bacterial expression of AsCas12a D908A (RuvC-inactivating mutation) with an N-terminal His-MBP tagDepositorInsertAsCas12a D908A
UseTags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged aspartate 908 to alaninePromoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas12a-A3B
Plasmid#193642PurposeExpression of base editor dCas12a-A3B in mammalian cells for base editing, along with non-fused EGFP fluorescenceDepositorInsertdLbCas12a-hA3B, EGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas12a-A3G
Plasmid#193643PurposeExpression of base editor dCas12a-A3G in mammalian cells for base editing, along with non-fused EGFP fluorescenceDepositorInsertdLbCas12a-hA3G, EGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
FnoCas12a-2C-NLS in pCSDest
Plasmid#126639PurposeExpresses FnoCas12a-2C-NLS in mammalian cellsDepositorInsertFnoCas12a-2C-NLS
UseCRISPRTags3xHuman influenza hemagglutinin (HA)-TagExpressionMammalianMutationPromoterCMV IE94 promoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only