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pBabe 3XFLAG-S/T3A-GATA6-3XAU1 puro

Plasmid

#72611

Purpose

For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with triple alanine mutations on aminoacids#33,#34 and #37

Depositor

Kevin Janes

Article

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

GATA binding protein 6 (GATA6 Human)

Use

Retroviral

Tags

3XAU1 and 3XFLAG

Expression

Mammalian

Mutation

changed serine33, threonine34 and serine37 to ala…

Promoter

Available since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only
p53 (dominant negative R175H mutant)-pcw107-V5

Plasmid

#64638

Purpose

3rd generation lentiviral transfer plasmid. When used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

TP53 (transcript variant 3) (TP53 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

R175H

Promoter

PGK

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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myr-FLAG-AKT1-pcw107

Plasmid

#64606

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

AKT1 (AKT1 Human)

Use

Lentiviral

Tags

FLAG and myristoylated

Expression

Mutation

Promoter

PGK

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
beta-catenin (S33A, S37A, T41A, S45A)-pcw107-V5

Plasmid

#64613

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

Ctnnb1 (Ctnnb1 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mutation

S33A, S37A, T41A, S45A

Promoter

PGK

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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Kras (G12V)-pcw107

Plasmid

#64602

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

KRAS (KRAS Human)

Use

Lentiviral

Tags

Expression

Mutation

G12V

Promoter

PGK

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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beta-catenin (S33Y)-pcw107

Plasmid

#64615

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

CTNNB1 (CTNNB1 Human)

Use

Lentiviral

Tags

Expression

Mutation

S33Y

Promoter

PGK

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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beta-catenin (S33A, S37A, T41A, S45A)-pcw107

Plasmid

#64612

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

Ctnnb1 (Ctnnb1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

S33A, S37A, T41A, S45A

Promoter

PGK

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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beta-catenin (S33Y)-pcw107-V5

Plasmid

#64616

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

CTNNB1 (CTNNB1 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

S33Y

Promoter

PGK

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
SmoM2 (W535L)-pcw107-V5

Plasmid

#64628

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

SMO (SMO Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

W535L

Promoter

PGK

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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Notch1 intracellular domain-pcw107-V5

Plasmid

#64622

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

NOTCH1 (NOTCH1 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

intracellular domain *

Promoter

PGK

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only
AR-V7-pcw107

Plasmid

#64635

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

AR (transcript variant 1, splice isoform) (AR Human)

Use

Lentiviral

Tags

Expression

Mutation

splice isoform*

Promoter

PGK

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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MEK1 (S218D,S222D)-pcw107

Plasmid

#64604

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

MAP2K1 (MAP2K1 Human)

Use

Lentiviral

Tags

Expression

Mutation

S218D, S222D

Promoter

PGK

Available since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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BCL-XL-pcw107-V5

Plasmid

#64631

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

BCL2L1 (transcript variant 1) (BCL2L1 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

PGK

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
IKKalpha (S176E,S180E)-pcw107-V5

Plasmid

#64608

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

CHUK (CHUK Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

S176E, S180E

Promoter

PGK

Available since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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AR-V7-pcw107-V5

Plasmid

#64636

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

AR (transcript variant 1, splice isoform) (AR Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

splice isoform *

Promoter

PGK

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
Hras (G12V)-pcw107

Plasmid

#64603

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

HRAS (HRAS Human)

Use

Lentiviral

Tags

Expression

Mutation

G12V

Promoter

PGK

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
MEK5 DD(S311D,T315D)-pcw107-V5

Plasmid

#64619

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

MAP2K5 (MAP2K5 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

S311D, T315D*

Promoter

PGK

Available since

June 1, 2015

Availability

Academic Institutions and Nonprofits only
Notch1 intracellular domain-pcw107

Plasmid

#64621

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

NOTCH1 (NOTCH1 Human)

Use

Lentiviral

Tags

Expression

Mutation

intracellular domain *

Promoter

PGK

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
p38 WT O/E (MAPK14)-pcw107-V5

Plasmid

#64624

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

MAPK14 (transcript variant 2) (MAPK14 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

PGK

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
pBABE_hygro_mRFP1_NRF2(C506S, L4A)

Plasmid

#136580

Purpose

mammalian expression of mRFP1-tagged human NRF2(C506S, Leuzip mut)

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

NRF2 (C506S, Leuzip mut) (NFE2L2 Human)

Use

Retroviral

Tags

N-mRFP1-FLAG

Expression

Mammalian

Mutation

The C506S mutation (C513S in the actual sequence …

Promoter

Available since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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