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Plasmid#78920PurposeMammalian expression of RNF138 with an EGFP fusionDepositorInsertRNF138 (RNF138 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR-U6gRNA-PGKpuro2APDGFB
Plasmid#136409PurposeDonor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses PDGFB.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationBbsI restriction site in the PDGFB sequence (Glu1…PromoterU6, PDKAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJC5-4-insulator-BREreporter-insulator
Plasmid#171499Purposeinjections to make Bmp reporter mouse transgenic lineDepositorInsertInsulator BRE-MLP:H2B-Turqoise
UseTagsExpressionMammalianMutationPromoterBRE-MLPAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
VanGlow-RR-DSCP
Plasmid#83339Purposefor Gateway cloning of enhancer elements upstream of the DSCP and mCherry::Renilla(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.DepositorTypeEmpty backboneUseLuciferaseTagsmCherry::Renilla(nls)ExpressionInsectMutationPromoterDrosophila Synthetic Core PromoterAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
GW1-mito-roGFP1-mApple
Plasmid#107218PurposeMammalian expression plasmid to target the roGFP1-mApple sensor to the mitochondrial matrix.DepositorInsertroGFP1-mApple
UseTags4xCox8ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2-FLAG-hEcto/Tif1g-CAmut
Plasmid#20903DepositorInsertEctodermin (TRIM33 Human)
UseTagsFLAGExpressionMammalianMutationPoint mutagenesis of C125 and C128 to Alanine (RI…PromoterAvailable sinceMay 15, 2009AvailabilityAcademic Institutions and Nonprofits only -
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GW1-mito-mCherry-roGFP2
Plasmid#107220PurposeMammalian expression plasmid to target the mCherry-roGFP2 sensor to the mitochondrial matrix.DepositorInsertmCherry-roGFP2
UseTags4xCox8ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
GW1-mito-mRuby2-roGFP1
Plasmid#107219PurposeMammalian expression plasmid to target the rmRuby1-roGFP1 sensor to the mitochondrial matrix.DepositorInsertmRuby2-roGFP1
UseTags4xCox8ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
FUW-TetO-Tfap2c
Plasmid#128826Purposedox-dependent expression of mouse Tfap2cDepositorInsertTfap2c (Tfap2c Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
VanGlow-GL-DSCP
Plasmid#83338Purposefor Gateway cloning of enhancer elements upstream of the DSCP and GFP::Luciferase(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.DepositorTypeEmpty backboneUseLuciferaseTagsGFP::Luciferase(nls)ExpressionInsectMutationPromoterDrosophila Synthetic Core PromoterAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-dLight1.2
Plasmid#111069PurposeTo generate Adeno-Associated viruses for expression of dLight1.1 under CAG promoterDepositorHas ServiceAAV1InsertdLight1.2
UseAAVTagsFlag tagExpressionMutationPromoterAvailable sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pONSY-CoNMM:mCherry
Plasmid#111878PurposeThis plasmid expresses mCherry fluorescent protein fused to an N-Myristoylation motif (NMM) from the endogenous Src2 gene (CAOG_06360). It can be used to visualize the membrane and filopodia.DepositorInsertCapsaspora N-Myristoylation motif (CoNMM) from the Src2 gene fused to mCherry
UseCapsaspora owczarzakiTagsmCherryExpressionMutationPromoterElongation Factor 1 alpha (EF1a) from CapsasporaAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR-U6gRNA-PGKpuro2ABFP
Plasmid#136405PurposeDonor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses Puromycin linked to TagBFP.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationBbsI restriction site at position 437 was removed…PromoterU6, PDKAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PM-InPAkt
Plasmid#181915PurposeIndicator of Phosphoinositides using Akt; targeted to the plasma membrane.DepositorInsertpm-InPAkt
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationContains the following mutations with respect to …PromoterCMVAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC7A3
Plasmid#132301PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC7A3 (SLC7A3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEGFP-RNF138-delta 18-58
Plasmid#78921PurposeMammalian expression of RNF138 with delta 18-58 and an EGFP fusionDepositorInsertRNF138 (RNF138 Human)
UseTagsEGFPExpressionMammalianMutationdelta 18-58PromoterCMVAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pONSY-CoH2B:Venus
Plasmid#111877PurposeThis plasmid expresses Venus fluorescent protein fused to endogenous Histone H2B (H2B) gene of Capsaspora (CAOG_01818). It can be used to transfect Capsaspora cells and visualize nucleus in vivo.DepositorInsertCapsaspora Histone H2B (CoH2B) fused to Venus
UseCapsaspora owczarzakiTagsVenusExpressionMutationPromoterElongation Factor 1 alpha (EF1a) from CapsasporaAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNTKV- Utf1-KI-3'UTR-2A-tdTomato-RBGpA
Plasmid#128834PurposeKnock-in of tdTomato into mouse Utf1 locusDepositorInserttdTomato into Utf1 locus (Utf1 Mouse)
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV-REX-GECO0.9
Plasmid#61247PurposeExpresses REX-GECO0.9 in mammalian cellsDepositorInsertREX-GECO0.9
UseTagsExpressionMammalianMutationSubstitutions relative to R-GECO1: P60R, V61W, R6…PromoterCMVAvailable sinceMarch 27, 2015AvailabilityAcademic Institutions and Nonprofits only