We narrowed to 24,628 results for: Nes

1. pJT11

   Plasmid

   #98308

   Purpose

   Overexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Citrus limon limonene synthase LIS1 under the control of GAL1/GAL2 promoter

   Depositor

   Claudia Vickers

   Article

   Peng et al Metab Eng. 2018 Feb 19. pii: S1096-7176(

   Insert

   TRPL3ClLIS1>TRPL41B
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Nano-lantern-plasma membrane/pcDNA3

   Plasmid

   #51975

   Purpose

   mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, targeted to the plasma membrane

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern
   

   Use

   Tags

   HIS and Lyn

   Expression

   Mammalian

   Mutation

   S257G in Rluc8

   Promoter

   Available Since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pP19 (GB0038)

   Plasmid

   #68195

   Purpose

   Provides the CDS of the Tomato bushy stunt virus (TBSV) silencing suppressor P19 as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   P19 coding region
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available Since

   Oct. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCB128

   Plasmid

   #205137

   Purpose

   Expression of mCherry-2xFYVE in Dictyostelium

   Depositor

   Jason King

   Article

   Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

   Insert

   2xFYVE (from human Hrs) (HGS Synthetic)
   

   Use

   Dictyostelium expression

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Orange Nano-lantern/pRSETB

   Plasmid

   #67659

   Purpose

   orange luminescent protein for high-speed single-cell and whole body imaging

   Depositor

   Takeharu Nagai

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   Orange Nano-lantern
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pCfB3034(X-3 MarkerFree)

   Plasmid

   #73272

   Purpose

   EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-3 (Chr X: 223616..224744)

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pENTR221-PGK.Pro

   Plasmid

   #79489

   Purpose

   MultiSite Gateway entry clones, first fragment, (attL1, attR5) Human PGK promoter

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   PGK Promoter (PGK1 Human, Synthetic)
   

   Use

   Multisite gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   PGK

   Available Since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. ptdEos-CLC

   Plasmid

   #38009

   Depositor

   Xiaowei Zhuang

   Article

   Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

   Inserts

   Clathrin, light polypeptide (LCa) (Clta Mouse)
   Eos FP, tandem dimer
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GOMO-GFP-LUC

   Plasmid

   #60976

   Purpose

   Rapid selection of recombinant marker-free bioluminescent rodent malaria parasites

   Depositor

   Olivier Silvie

   Article

   Manzoni et al Sci Rep. 2014 Apr 23;4:4760. doi: 10.103

   Inserts

   GFP-Luciferase fusion
   mCherry
   hDHFR-yFCU
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pU6-26 (GB1001)

    Plasmid

    #68208

    Purpose

    Provides the A. thaliana U6-26 RNA polIII promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    AtU6-26 promoter
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Cyan Nano-lantern/pRSETB

    Plasmid

    #67658

    Purpose

    cyan luminescent protein for high-speed single-cell and whole body imaging

    Depositor

    Takeharu Nagai

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Cyan Nano-lantern
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

12. Cyan Nano-lantern(Ca2+)/pcDNA3

    Plasmid

    #71344

    Purpose

    Express Cyan Nano-lantern-based luminescent Ca2+ indicator in mammalian cell

    Depositor

    Takeharu Nagai

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Cyan Nano-lantern(Ca2+)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pHsp18.2 (GB0153)

    Plasmid

    #68171

    Purpose

    Provides the Arabidopsis thaliana heat shock protein 18.2 promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    AtHsp18.2 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEGB Tnos:NptII:Pnos (GB0226)

    Plasmid

    #68212

    Purpose

    Transcriptional unit for kanamycin resistance gene plant expression

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    NptII
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos

    Available Since

    Oct. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHsp70B (GB0155)

    Plasmid

    #68172

    Purpose

    Provides the Arabidopsis thaliana heat shock protein 70B promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    AtHsp70B promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-myc-ΔD-box-CycA-GFP (525)

    Plasmid

    #39856

    Depositor

    Jonathon Pines

    Article

    den Elzen et al J Cell Biol. 2001 Apr 2;153(1):121-36.

    Insert

    Cyclin A2 (CCNA2 Human)
    

    Use

    Tags

    GFP and Myc

    Expression

    Mammalian

    Mutation

    L2V; Δ destruction box = deletion of aa47-55

    Promoter

    Available Since

    Jan. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCfB2899(X-2 MarkerFree)

    Plasmid

    #73271

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-2 (Chr X: 194944..195980)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDelila (GB0079)

    Plasmid

    #68200

    Purpose

    Provides the CDS of the Antirrhinum majus Delila transcription factor as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Delila coding region
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRosea (GB0026)

    Plasmid

    #68194

    Purpose

    Provides the CDS of the Antirrhinum majus Rosea transcription factor as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    A. majus Rosea coding region
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    Oct. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHygroR-GMRWhite

    Plasmid

    #165886

    Purpose

    Hygromycin resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Transgenesis Ready Selection Vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRenilla (GB0056)

    Plasmid

    #68198

    Purpose

    Provides the CDS of the Renilla protein as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Renilla coding region
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. EGFP-GEM

    Plasmid

    #111191

    Purpose

    fluorescent fusion protein

    Depositor

    Johannes A. Schmid

    Article

    Piddini et al EMBO J. 2001 Aug 1. 20(15):4076-87.

    Insert

    GEM (GEM Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    T2A

    Promoter

    Available Since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. CLM RP HTR

    Plasmid

    #24430

    Depositor

    Ron Vale

    Article

    Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

    Insert

    Kinesin (KIF5B Human)
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    C7S/C65A/C168A/C174S/C294A/C330S/C421A

    Promoter

    Available Since

    June 30, 2010

    Availability

    Academic Institutions and Nonprofits only

24. pEYFP-Pds1Δdestruction box(383)

    Plasmid

    #39848

    Depositor

    Jonathon Pines

    Article

    Hagting et al J Cell Biol. 2002 Jun 24;157(7):1125-37.

    Insert

    Pds1 (PTTG1 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Δdestruction box = ΔR61-N68

    Promoter

    Available Since

    Oct. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBI101.1/ProGOXL1:GUS

    Plasmid

    #175557

    Purpose

    Binary vector for Agrobacterium-mediated plant transformation - Promoter:GUS reporter for expression analysis of GOXL genes

    Depositor

    George Haughn

    Article

    Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

    Insert

    ProGOXL1
    

    Use

    Tags

    GUS

    Expression

    Plant

    Mutation

    Promoter

    GOXL1

    Available Since

    Jan. 13, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. KAP3-CT

    Plasmid

    #51396

    Purpose

    expression of EGFP-tagged COOH-terminal AA 661–793 KAP3B in mammalian cells

    Depositor

    Trina Schroer

    Article

    Brown et al Traffic. 2005 Dec;6(12):1114-24.

    Insert

    Kinesin-associated protein 3 (KIFAP3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

27. pSLIK_Zeo_3xflag_Luciferase

    Plasmid

    #136533

    Purpose

    Lentiviral expression vector for an inducible 3xflag-tagged Luciferase

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    Luciferase
    

    Use

    Lentiviral; Destinatioin vector for gateway cloni…

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEGB Pnos:Luciferase:Tnos (GB0112)

    Plasmid

    #68217

    Purpose

    Transcriptional unit for Firefly Luciferase Protein plant expression driven by the Nos promoter

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Luc
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos

    Available Since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA-3xflagGREB1b

    Plasmid

    #112222

    Purpose

    mammalian expression of flag tagged GREB1b

    Depositor

    Craig Burd

    Article

    Haines et al Endocr Relat Cancer. 2018 Jul;25(7):735-

    Insert

    GREB1b (GREB1 Human)
    

    Use

    Tags

    3x flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEN_TT Tgfbr3-HA

    Plasmid

    #58497

    Purpose

    Gateway entry vector for an inducible HA-tagged mouse Tgfbr3

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    Tgfbr3_HA (Tgfbr3 Mouse)
    

    Use

    Entry vector

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

31. hCMV-IE1:Lucia:bgH

    Plasmid

    #135293

    Purpose

    Transcriptional unit for constitutive expression of Lucia luciferase in mammalian cells

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    CMV:Lucia:bGH
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pSU314

    Plasmid

    #169696

    Purpose

    Template plasmid for generating C-terminal hemagglutinin (1xHA) epitope tag fusions to bacterial genes by "Lambda red" recombineering.

    Depositor

    Nara Figueroa

    Article

    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98

    Insert

    1xHA epitope tag adjacent to FRT-flanked cat (CamR) gene
    

    Use

    Tags

    1xHA tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pOpLexA::mini35s (GB0733)

    Plasmid

    #68180

    Purpose

    Provides the 4xLexA Operator fused to CaMV 35s minimal promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    4xOpLexA:mini35S
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pXylA-RgTALsyn

    Plasmid

    #100948

    Purpose

    Expression of Tyrosine Ammonia Lyase from constitutive XylA promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

    Insert

    RgTALsyn
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Silent mutation to remove internal NdeI site on T…

    Promoter

    XylA (Constitutive)

    Available Since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pSU312

    Plasmid

    #169694

    Purpose

    Template plasmid for generating C-terminal 1xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.

    Depositor

    Nara Figueroa

    Article

    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98

    Insert

    1xFLAG epitope tag adjacent to FRT-flanked aph (KanR) gene
    

    Use

    Tags

    1xFLAG tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSU313

    Plasmid

    #169695

    Purpose

    Template plasmid for generating C-terminal 1xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.

    Depositor

    Nara Figueroa

    Article

    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98

    Insert

    1xFLAG epitope tag adjacent to FRT-flanked cat (CamR) gene
    

    Use

    Tags

    1xFLAG tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pDGB1omega2_SF (GB0466)

    Plasmid

    #68243

    Purpose

    Twister plasmid to swap inserts from an omega1 or omega1R vector to any alpha level vector

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Solanum lycopersicum intergenic region
    

    Use

    Synthetic Biology; Unspecified

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    Oct. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pDGB1alpha2_SF (GB0107)

    Plasmid

    #68233

    Purpose

    Twister plasmid to swap inserts from an alpha1 or alpha1R vector to any omega level vector

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Solanum lycopersicum intergenic region
    

    Use

    Synthetic Biology; Unspecified

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    Oct. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. AAV-CAG-DIO-mAPX

    Plasmid

    #79908

    Purpose

    Adeno-associated virus plasmid, Cre-dependent FLEX switch, mAPX

    Depositor

    Joshua Sanes

    Article

    Joesch et al Elife. 2016 Jul 7;5. pii: e15015. doi: 1

    Insert

    mAPX
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCMV-HA-mIFITM1-C49,50,83,103A

    Plasmid

    #58421

    Purpose

    Expresses murine IFITM1-C49,50,83,103A with an N-terminal HA tag in mammalian cells (cysteine-less mutant that is not palmitoylated).

    Depositor

    Jacob Yount

    Article

    Hach et al J Virol. 2013 Sep;87(17):9923-7. doi: 10

    Insert

    IFITM1 (Ifitm1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Cysteines 49, 50, 83, and 103 to Alanine

    Promoter

    CMV IE

    Available Since

    Sept. 9, 2014

    Availability

    Academic Institutions and Nonprofits only