We narrowed to 6,546 results for: proc

1. pcDNA3.tgoGAP1

   Plasmid

   #78737

   Purpose

   Expression of Ca2+ sensor GAP1 in the trans Golgi apparatus

   Depositor

   Teresa Alonso

   Article

   Rodriguez-Garcia et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

   Insert

   tgoGAP1
   

   Use

   Tags

   N terminal 81 amino acids of human galactosyltran…

   Expression

   Mammalian

   Mutation

   D117A/D119A/D163A in aequorin moiety

   Promoter

   CMV

   Available since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA4-ATR-N

   Plasmid

   #53767

   Purpose

   mammalian expression of flag-tagged ATR N-terminal fragment

   Depositor

   Aziz Sancar

   Article

   Unsal-Kacmaz et al Proc Natl Acad Sci U S A. 2002 May 14;99

   Insert

   ATR (ATR Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   N-terminal domain, aa 1-1409

   Promoter

   CMV

   Available since

   June 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Arl6ip1-mEmerald

   Plasmid

   #186965

   Purpose

   Express Arl6ip1 with C-terminus mEmerald

   Depositor

   Ke Xu

   Article

   Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

   Insert

   ARL6IP1 (ARL6IP1 Human)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CMV Promoter

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFastBac-hDicer

   Plasmid

   #89144

   Purpose

   Expression of hDicer in Sf9 Cells

   Depositor

   Jennifer Doudna

   Article

   MacRae et al Proc Natl Acad Sci U S A. 2008 Jan 15;10

   Insert

   hDicer (DICER1 Human)
   

   Use

   Tags

   His6-tev

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGEX4T-1_WT_OCT4

   Plasmid

   #40633

   Depositor

   James Thomson

   Article

   Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

   Insert

   POU5F1 (POU5F1 Human)
   

   Use

   Tags

   GST, TEV cleavage, and Thrombin cleavage

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   July 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA4-ATR-M

   Plasmid

   #53768

   Purpose

   mammalian expression of flag-tagged ATR middle region

   Depositor

   Aziz Sancar

   Article

   Unsal-Kacmaz et al Proc Natl Acad Sci U S A. 2002 May 14;99

   Insert

   ATR (ATR Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   middle domain, aa 593-1700*

   Promoter

   CMV

   Available since

   July 8, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pBIG2ab

   Plasmid

   #80616

   Purpose

   pBIG2ab - Step2 vector ab (biGBac system for expression of protein complexes in insect cells)

   Depositor

   Jan-Michael Peters

   Article

   Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AWP-031

   Plasmid

   #213967

   Purpose

   Expresses dPb2Cas12a under an IPTG-inducible promoter and contains a cassette for constitutive CRISPR array expression.

   Depositor

   Alexander Westermann

   Article

   Prezza et al Proc Natl Acad Sci U S A. 2024 Feb 6;121

   Insert

   Cas12a (cas12a Synthetic, Segatella bryantii)
   

   Use

   Tags

   3XFLAG

   Expression

   Bacterial

   Mutation

   D875A to inactivate target nuclease activity

   Promoter

   PcfxA, IPTG inducible

   Available since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA4-Beclin1-HA

   Plasmid

   #24399

   Depositor

   Qing Zhong

   Article

   Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

   Insert

   Beclin1 (BECN1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pWPT-/GCCACC-mEGFP-IRES-mCherry

    Plasmid

    #49235

    Purpose

    Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

    Depositor

    Clifford Wang

    Article

    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

    Inserts

    mEGFP
    mCherry
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    SfuI site to create C-terminal fusions

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-short

    Available since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHeACT-mEGFP-NLS

    Plasmid

    #205003

    Purpose

    The vector contains 1kbp of the upstream and downstream regions of the actin gene from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.

    Depositor

    Kazuharu Arakawa

    Article

    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

    Insert

    mEGFP-NLS flanked by 1kbp upstream and downstream of the actin gene from Hypsibius exemplaris
    

    Use

    Tardigrade expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-AU1-mTOR-E2419K

    Plasmid

    #19994

    Depositor

    Fuyuhiko Tamanoi

    Article

    Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

    Insert

    mammalian target of rapamycin-E2419K (Mtor Rat)
    

    Use

    Tags

    AU1

    Expression

    Mammalian

    Mutation

    Changed Glutamine 2419 to Lysine

    Promoter

    Available since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pPRIME-TET-GFP-FF3

    Plasmid

    #11662

    Purpose

    3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a tetracycline-responsive promoter (TET) controlling expression of GFP and miR30-based shRNA targeting firefly luciferase

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

    Insert

    FF3
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GFP-Rabenosyn-5

    Plasmid

    #37538

    Depositor

    Silvia Corvera

    Article

    Navaroli et al Proc Natl Acad Sci U S A. 2012 Feb 21;10

    Insert

    Rabenosyn5 (RBSN Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA5-H2B-AID-EYFP

    Plasmid

    #47329

    Purpose

    for plasmid integration using the Flp-In system. AID at C-terminal

    Depositor

    Don Cleveland

    Article

    Holland et al Proc Natl Acad Sci U S A. 2012 Dec 4;109

    Insert

    histone H2B (H2BC21 Human)
    

    Use

    Tags

    AID and EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHAGE-UBC-TagRFP-T-ps

    Plasmid

    #178656

    Purpose

    Expression of palmitoylated TagRFP-T for live imaging of plasma membrane (protrusions, tunneing nanotubes), in mammalian cells

    Depositor

    Jeffrey Gerst

    Article

    Haimovich et al Proc Natl Acad Sci U S A. 2017 Nov 14;11

    Insert

    TagRFP-T with palmitoylation signal at N-terminus
    

    Use

    Lentiviral

    Tags

    Palmitoylation signal

    Expression

    Mutation

    Promoter

    Ubc

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FLAG-ELL pBacPAK8

    Plasmid

    #15322

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

    Insert

    eleven nineteen lysine rich in leukemia (ELL Human)
    

    Use

    Baculovirus construction

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pDONR TDP43 WT YFP

    Plasmid

    #27470

    Depositor

    Aaron Gitler

    Article

    Johnson et al Proc Natl Acad Sci U S A. 2008 Apr 29. 1

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET21 MBPx-SpyCatcher

    Plasmid

    #72327

    Purpose

    Bacterial expression of tandem high affinity maltose binding protein domains linked to SpyCatcher, for solid-phase polyprotein synthesis.

    Depositor

    Mark Howarth

    Article

    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

    Insert

    pET21 MBPx-SpyCatcher
    

    Use

    Tags

    N-terminal His6 tag

    Expression

    Bacterial

    Mutation

    MBP contains A312V, I317V mutations and 172, 173,…

    Promoter

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MELOPS

    Plasmid

    #80151

    Purpose

    Genetically encoded melanosome-localized pH sensor.

    Depositor

    Santiago Di Pietro

    Article

    Ambrosio et al Proc Natl Acad Sci U S A. 2016 May 17;11

    Insert

    OCA2 (OCA2 Human)
    

    Use

    Tags

    mNectarine in first luminal loop

    Expression

    Mammalian

    Mutation

    nonpathologic polymorphism D257A, pore mutation V…

    Promoter

    CMV

    Available since

    Sept. 20, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits