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We narrowed to 70,504 results for: LAS

Showing: 3001 - 3030 of 70504 results

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pAAV-CMVd1-NLS-GAL4-iLight2-VP16

Plasmid

#219550

Purpose

NLS-Gal4(1-65)-iLight2-VP16 construc expression in mammalian cells. pAAV vector.

Depositor

Vladislav Verkhusha

Article

Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

Insert

NLS-Gal4(1-65)-iLight2-VP16

Use

AAV

Tags

Expression

Mammalian

Mutation

The (SAGG)x4 linker between GAL4(1-65) and the PC…

Promoter

CMVd1

Available since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pACUH-GFP11x7-mCherry-α-tubulin

Plasmid

#70218

Purpose

Expresses GFP11x7-mCherry-α-tubulin in insect cells. 5 a. a. linker lengths.

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

GFP11x7-mCherry-α-tubulin

Use

Tags

GFP11x7 and mCherry

Expression

Insect

Mutation

Promoter

Available since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a-mTurquoise2-CNA35

Plasmid

#61602

Purpose

bacterial expression of N-terminal fusion protein of CNA35 with m-Turquoise-2

Depositor

Maarten Merkx

Article

Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

Insert

mTurqouise and CNA35

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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TR-Csy4-R115A/R119A

Plasmid

#80696

Purpose

Expresses Csy4 with R115A and R119A mutations from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

Depositor

Aravind Asokan

Article

Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

Insert

Csy4-R115A/R119A

Use

AAV

Tags

Expression

Mammalian

Mutation

Arg115 and Arg119 both mutated to Ala

Promoter

CBA

Available since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pFast-Flag-hRad9

Plasmid

#222468

Purpose

Expresses hRad9 in insect cells

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al Proc Natl Acad Sci U S A. 2001 Sep 25;98

Insert

hRad9 (RAD9A Human)

Use

Tags

Flag epitope

Expression

Insect

Mutation

Promoter

Available since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-TRE3G-PYL1-sfGFP-PML

Plasmid

#167011

Purpose

expression of PYL1-SfGFP-PML, lentivirus vector

Depositor

Stanley Qi

Article

Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

Insert

PML (PML Human)

Use

Lentiviral

Tags

GFP-PML

Expression

Mammalian

Mutation

Promoter

Available since

June 13, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-mAmetrine-CNA35

Plasmid

#61604

Purpose

bacterial expression of N-terminal fusion protein of CNA35 with mAmetrine

Depositor

Maarten Merkx

Article

Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

Insert

mAmetrine and CNA35

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a-LSSmOrange-CNA35

Plasmid

#61605

Purpose

bacterial expression of N-terminal fusion protein of CNA35 with LSSmOrange

Depositor

Maarten Merkx

Article

Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

Insert

LSSmOrange and CNA35

Use

Tags

His tag

Expression

Mutation

Promoter

T7

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pFast-His6-hRad17

Plasmid

#222465

Purpose

Expresses hRad17 in insect cells

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al Proc Natl Acad Sci U S A. 2001 Sep 25;98

Insert

hRad17 (RAD17 Human)

Use

Tags

His

Expression

Insect

Mutation

Promoter

Available since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pRS156

Plasmid

#182707

Purpose

CRISPRa plasmid for Candida albicans (dCas9-VPR and sgRNA cloning site)

Depositor

Rebecca Shapiro

Article

Gervais et al G3 (Bethesda). 2022 Dec 1:jkac301. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pX330-EN479

Plasmid

#86234

Purpose

For transient expression of spCas9-nuclease and a sgRNA targeting the mouse Rosa26 locus

Depositor

Benoit Bruneau

Article

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

spCas9-nickase and sgRNA against mouse rosa26 locus

Use

Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-VPS15

Plasmid

#99326

Purpose

Component for mammalian expression of recombinant PI3KC3-C1 complex

Depositor

James Hurley

Article

Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

Insert

VPS15 (PIK3R4 Human)

Use

Tags

Strep-Strep-Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only
pCAG-OSF-VPS34

Plasmid

#99327

Purpose

Component for mammalian expression of recombinant PI3KC3-C1 complex

Depositor

James Hurley

Article

Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

Insert

VPS34 (PIK3C3 Human)

Use

Tags

Strep-Strep-Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only
pET-16b-10His-TEV-MCES_VACCA_D1_D12

Plasmid

#203671

Purpose

The D1 and D12 subunits of the vaccinia capping enzyme were cloned in tandem into the vector pET-16b (Novagen). An N-terminal His10 tag was added to the D1 subunit and the original factor Xa cleavage

Depositor

Stephen Cusack

Article

Kyrieleis et al Structure. 2014 Mar 4;22(3):452-65. doi:

Insert

MCES_VACCA_D1_D12

Use

Tags

10xHis

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pEN716 - pCAGGS-eGFP-3Xnls-bpA

Plasmid

#156435

Purpose

For transient expression of nuclear eGFP in mammalian cells

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

eGFP

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-G12-mFGF2

Plasmid

#219552

Purpose

mFGF2 expression in mammlian cells. pAAV vector. Reporter plasmid.

Depositor

Vladislav Verkhusha

Article

Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

Insert

mFGF2 (Fgf2 Mouse)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

12xUAS with TATA-box minimal promoter

Available since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-U6-sgTel-CMV-puro-P2A-mCherry-TRF1

Plasmid

#169449

Purpose

expression of TRF1-mcherrry and sgRNA targeting telomeres

Depositor

Stanley Qi

Article

Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

Insert

mCherry-TRF1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti_EF1a_EV

Plasmid

#183116

Purpose

Empty vector control for pLenti_EF1a constructs

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

Small multiple cloning site

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pGal4AD-CIB1

Plasmid

#28245

Purpose

Expresses Gal4 Activation Domain fused to CIB1 for blue-light-induced transcription activation

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

Gal4AD (CIB1, GAL4 Budding Yeast)

Use

Tags

CIB1

Expression

Yeast

Mutation

Promoter

ADH1

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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CAG-FLEx(FRT)-TC

Plasmid

#67827

Purpose

Flp-dependent expression of TVA-mCherry fusion to mediate modifed rabies virus expression in cells

Depositor

Liqun Luo

Article

Schwarz et al Nature. 2015 Aug 6;524(7563):88-92. doi:

Insert

TVA-mCherry fusion

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pCRY2FL(deltaNLS)-mCherryN1

Plasmid

#26871

Purpose

Mammalian expression of cytoplasmic CRY2-mCherry

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

CRY2 (CRY2 Mustard Weed)

Use

Tags

mCherry

Expression

Mammalian

Mutation

NLS sequences deleted

Promoter

CMV

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pGal4BD-CRY2PHR

Plasmid

#28244

Purpose

Expresses Gal4 DNA Binding Domain fused to CRY2PHR (aa1-498) for blue-light-induced transcription activation

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

cry2 (CRY2 Mustard Weed)

Use

Tags

Gal4BD

Expression

Yeast

Mutation

Promoter

Available since

March 23, 2011

Availability

Academic Institutions and Nonprofits only

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pGal4BD-CRY2

Plasmid

#28243

Purpose

Expresses Gal4 DNA Binding Domain fused to CRY2 for blue-light-induced transcription activation

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

CRY2 (CRY2 Mustard Weed)

Use

Tags

Gal4BD

Expression

Yeast

Mutation

Promoter

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pCIB1(deltaNLS)-pmGFP

Plasmid

#28240

Purpose

Expresses CIB1 fused to plasma membrane-localized EGFP

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

CIB1(deltaNLS)-pmGFP (CIB1 Mustard Weed)

Use

Tags

EGFP

Expression

Mammalian

Mutation

NLS sequences mutated

Promoter

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR_zeo_sgNT135

Plasmid

#183181

Purpose

pLentiCRISPR that is selectable with zeocin with a non-targeting sgRNA

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

NT135

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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CAG-FLEx(FRT)-G

Plasmid

#67828

Purpose

Flp-dependent expression of rabies glycoprotein to mediate trans-synaptic spread of modifed rabies virus in cells

Depositor

Liqun Luo

Article

Schwarz et al Nature. 2015 Aug 6;524(7563):88-92. doi:

Insert

rabies glycoprotein

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pDM002-sgRNA-lenti-ZeoR

Plasmid

#200060

Purpose

Lentiviral vector for expression of sgRNA with mCherry, zeocin resistance, BlpI + BstXI cloning sites

Depositor

Britt Glaunsinger

Article

McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

Insert

Bleomycin resistance, mCherry

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-PDZD8HA_sh_resistant

Plasmid

#105006

Purpose

Mammalian Expression of PDZD8, sh resistant

Depositor

Franck Polleux

Article

Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

Insert

PDZD8 (Pdzd8 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

The sequence recognized by shPDZD8 (TRCN000043105…

Promoter

CAG

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pFastBac1 PIK3R1

Plasmid

#17743

Depositor

Bert Vogelstein

Article

Samuels et al Cancer Cell. 2005 Jun . 7(6):561-73.

Insert

PIK3R1 (PIK3R1 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only
CMV-PKCb1-mEGFP-N1

Plasmid

#112265

Purpose

ITRACKbeta/IDOCKSbeta Donor construct

Depositor

Ryohei Yasuda

Article

Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

Insert

protein kinase c beta (Prkcb Mouse)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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