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We narrowed to 18,057 results for: puro

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Showing: 3001 - 3030 of 18057 results

  4. mCherry-ActA-IRES-puromycin-pLVx-EF1a

    Plasmid
    #177433
    Purpose
    To express mCherry fused to ActA mitochondria targeting sequence. Lentiviral vector used to make cell lines expressing this mitochondria landmark.
    Depositor
    David Andrews
    Insert
    ActA tail anchor (actA Listeria monocytogenes)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177433-plasmid-map-sequence-id-348464

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  8. pLKO.1-puro U6 sgRNA BfuAI large stuffer

    Plasmid
    #52628
    Purpose
    lentiviral U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites, improved cassette cloning efficiency
    Depositor
    Scot Wolfe
    Insert
    Kanamycin cassette
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    April 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    52628-plasmid-map-sequence-id-217983

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  9. lenti sgRNA(MS2)_puro optimized backbone

    Plasmid
    #73797
    Purpose
    optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available Since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73797-plasmid-map-sequence-id-196924

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  15. lenti-EF1a-dCas9-KRAB-MeCP2-Puro

    Plasmid
    #242163
    Purpose
    Lentiviral expression of dCas9-KRAB-MeCP2 with puromycin reporter
    Depositor
    Amy Brock
    Insert
    dCas9-KRAB-MeCP2; T2A-puro
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    242163-plasmid-map-sequence-id-496076

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  17. pCEP-Puro-TetOn-L1RP-ORF1_R261A_Halo-GFPai

    Plasmid
    #202569
    Purpose
    Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the R261A mutation and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR
    Depositor
    Liam Holt
    Article
    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75
    Insert
    LINE-1
    Tags
    GFP-AI retrotransposition reporter and HaloTag7 o…
    Expression
    Mammalian
    Mutation
    Changed ORF1p Arginine 261 to Alanine
    Available Since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    202569-plasmid-map-sequence-id-429223

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  27. pCDH-EF1-E-'No'Mi-Red-T2A-Puro

    Plasmid
    #246642
    Purpose
    EF-1alpha driven EV reporter E-'No'Mi-R, a re-engineered tetraspanin scaffold tagged with bioluminescent and fluorescent reporter proteins. Nanoluc and Flag tag are removable with TEVp.
    Depositor
    Koen Breyne
    Article
    Obuchi et al J Extracell Vesicles. 2025 Jun;14(6):e70
    Insert
    cleavable E-NoMi-Red
    Use
    Lentiviral
    Tags
    FLAG-Nanoluciferase and mCherry
    Expression
    Mammalian
    Promoter
    EF-1alpha
    Available Since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    246642-plasmid-map-sequence-id-489517

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Showing: 3001 - 3030 of 18057 results