We narrowed to 24,628 results for: Nes

1. pTHsp18.2 (GB0035)

   Plasmid

   #68186

   Purpose

   Provides the Arabidopsis thaliana heat shock protein 18.2 terminator as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   AtHsp18.2 terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available Since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBBK12 pCAS-Tyr-[gRNA: 2=FF21] (SplitKanR, AmpR)

   Plasmid

   #178996

   Purpose

   Sp.Cas9 and gRNA yeast expression vector with FF21 gRNA pre-cloned. Selection =SplitKanamycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Nano-lantern-H2B/pcDNA3

   Plasmid

   #51971

   Purpose

   mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, fused to Histone H2B

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern
   

   Use

   Tags

   human H2B

   Expression

   Mammalian

   Mutation

   S257G in Rluc8

   Promoter

   Available Since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pBBK11 pCAS-Tyr-[gRNA: 1=FF18] (SplitKanR, AmpR)

   Plasmid

   #178995

   Purpose

   Sp.Cas9 and gRNA yeast expression vector with FF18 gRNA pre-cloned. Selection =SplitKanamycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pOpLacI:mini35ss (GB0534)

   Plasmid

   #68179

   Purpose

   Provides the 6xLacI Operon fused to CaMV 35S minimal promoter as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   6xOpLacI:mini35S
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available Since

   Oct. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. A337C

   Plasmid

   #24432

   Depositor

   Ron Vale

   Article

   Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

   Insert

   Kinesin (KIF5B Human)
   

   Use

   Tags

   6XHis and GFP

   Expression

   Bacterial

   Mutation

   C7S/C65A/C168A/C174S/C294A/C330S/C421A, A337C

   Promoter

   Available Since

   June 14, 2010

   Availability

   Academic Institutions and Nonprofits only

7. Venus-Cdc20 R132A (1768)

   Plasmid

   #26059

   Depositor

   Jonathon Pines

   Article

   Nilsson et al Nat Cell Biol. 2008 Dec . 10(12):1411-20

   Insert

   Cdc20 (CDC20 Human)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Mutation in the Mad2 binding site, R132A

   Promoter

   Available Since

   Aug. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

8. p6xAP-1_RE

   Plasmid

   #118054

   Purpose

   GBPart - Operator/DNA Response element - 6 copies of the AP-1 binding motif

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Insert

   6 copies of the AP-1 binding motif
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. YFP(1-158) in pcDNAI/Amp

   Plasmid

   #60888

   Purpose

   This vector attaches YFP(1-158) to the N-terminus of a protein.

   Depositor

   Catherine Berlot

   Article

   Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8

   Insert

   YFP(1-158)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Met was substituted for Gln-69.

   Promoter

   CMV

   Available Since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUPD_OsU3 (GB1184)

    Plasmid

    #68209

    Purpose

    Provides the O. sativa U3 RNA polIII promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    OsU3 promoter
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLKO.1 shPIK3CD.v1 puro

    Plasmid

    #58706

    Purpose

    Lentiviral shRNA vector for knockdown of human PIK3CD

    Depositor

    Kevin Janes

    Article

    Bajikar et al Proc Natl Acad Sci U S A. 2014 Feb 4;111

    Insert

    shPIK3CD (PIK3CD Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

12. TB:2xP53_RE:MiniP

    Plasmid

    #133881

    Purpose

    2 copies of the p53 DNA binding motif

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Insert

    2 copies of the p53 DNA binding motif
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPAtUbq10 (GB0223)

    Plasmid

    #68174

    Purpose

    Provides the Arabidopsis thaliana polyubiquitin 10 promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    AtUbq10 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. SEPLuc-IRES-Antares

    Plasmid

    #141085

    Purpose

    precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis

    Depositor

    Jeak Ling Ding

    Article

    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

    Insert

    SEPLuc-EMCV IRES-Antares
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pG418R-GMRWhite

    Plasmid

    #165883

    Purpose

    G418 resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Transgenesis Ready Selection Vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPAct2noUTR (GB0192)

    Plasmid

    #68177

    Purpose

    Provides the Arabidopsis thaliana actin2 gene promoter (without 5' UTR) as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    AtAct2 promoter (no UTR)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. YFP(159-238) in pcDNAI/Amp

    Plasmid

    #60889

    Purpose

    This vector attaches YFP(159-238) to the N-terminus of a protein.

    Depositor

    Catherine Berlot

    Article

    Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8

    Insert

    YFP(159-238)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSTAP-seq_fly-zfh1

    Plasmid

    #86381

    Purpose

    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.

    Depositor

    Alexander Stark

    Article

    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

    Insert

    D. melanogaster zfh1 enhancer
    

    Use

    Stap-seq screening vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHcPro (GB0067)

    Plasmid

    #68196

    Purpose

    Provides the CDS of the Tobacco echt virus HcPro protein as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    TEV HcPro coding region
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    Oct. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. K347CLM G234A/43/347

    Plasmid

    #24454

    Depositor

    Ron Vale

    Article

    Tomishige et al Nat Struct Mol Biol. 2006 Oct . 13(10):8

    Insert

    Kinesin (KIF5B Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    C7S/C65A/C168A/C174S/C294A/C330S, G234A/S43C/E3…

    Promoter

    Available Since

    July 15, 2010

    Availability

    Academic Institutions and Nonprofits only

21. Antares-SEPLuc

    Plasmid

    #141086

    Purpose

    precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis

    Depositor

    Jeak Ling Ding

    Article

    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

    Insert

    Antares-T2A-puromycin-EMCV IRES-SEPLuc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 66 to 91 on the Luciferas…

    Promoter

    CMV

    Available Since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pVenus-N1 cyclin B1 L45A (1324)

    Plasmid

    #61851

    Purpose

    mammalian expression of cyclin B1 L45 A mutant fused to Venus

    Depositor

    Jonathon Pines

    Article

    Matsusaka et al Biol Open. 2014 Sep 12;3(10):904-12. doi

    Insert

    cyclin B1 (CCNB1 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    L45A

    Promoter

    CMV

    Available Since

    Feb. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

23. pDGE76

    Plasmid

    #80578

    Purpose

    recipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, nptII

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Inserts

    pnos:nptII-tmos
    p2x35S:Cas9 D10A-tnos
    BsaI-ccdB_CmR-BsaI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A

    Promoter

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. mCerulean3-H2B-C-10

    Plasmid

    #55422

    Purpose

    Localization: Nucleus/Histones, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    H2B (H2BC11 Human)
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available Since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

25. pUC 3GLA UAS HAi

    Plasmid

    #71763

    Purpose

    gap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, Amp resistant, with 5xUAS and HA tag

    Depositor

    Matthias Soller

    Article

    Haussmann et al Nucleic Acids Res. 2018 Dec 12. pii: 523

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pOT2 3GLA

    Plasmid

    #71764

    Purpose

    gap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, chloramphenicol resistant

    Depositor

    Matthias Soller

    Article

    Haussmann et al Nucleic Acids Res. 2018 Dec 12. pii: 523

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTAtUbq3 (GB0219)

    Plasmid

    #68192

    Purpose

    Provides the Arabidopsis thaliana polyubiquitin 3 terminator as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    AtUbq3 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLKO.1 shPIK3CD.v2 puro

    Plasmid

    #58707

    Purpose

    Lentiviral shRNA vector for knockdown of human PIK3CD

    Depositor

    Kevin Janes

    Article

    Bajikar et al Proc Natl Acad Sci U S A. 2014 Feb 4;111

    Insert

    shPIK3CD (PIK3CD Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

29. pSTAP-seq_fly-tj

    Plasmid

    #86386

    Purpose

    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.

    Depositor

    Alexander Stark

    Article

    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

    Insert

    D. melanogaster tj enhancer
    

    Use

    Stap-seq screening vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PGL3-Ogt (-178 to +233 bp)

    Plasmid

    #79266

    Purpose

    To identify the Cis and Trans acting elements that control Ogt gene transcription.

    Depositor

    Steven Jones

    Article

    Muthusamy et al J Biol Chem. 2015 Dec 25;290(52):31013-2

    Insert

    Ogt promoter element (Ogt Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

31. K349CLM-6His 215/347

    Plasmid

    #24451

    Depositor

    Ron Vale

    Article

    Tomishige et al Nat Struct Mol Biol. 2006 Oct . 13(10):8

    Insert

    Kinesin (KIF5B Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    C7S/C65A/C168A/C174S/C294A/C330S, E215C, E347C

    Promoter

    Available Since

    July 15, 2010

    Availability

    Academic Institutions and Nonprofits only

32. A337C/Y344C

    Plasmid

    #24434

    Depositor

    Ron Vale

    Article

    Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

    Insert

    Kinesin (KIF5B Human)
    

    Use

    Tags

    6XHis and GFP

    Expression

    Bacterial

    Mutation

    C7S/C65A/C168A/C174S/C294A/C330S/C421A, A337C/Y…

    Promoter

    Available Since

    June 14, 2010

    Availability

    Academic Institutions and Nonprofits only

33. pDGE77

    Plasmid

    #80579

    Purpose

    recipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, BASTA

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Inserts

    pnos:PAT-tnos
    p2x35S:Cas9 D10A-tnos
    BsaI-ccdB_CmR-BsaI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A

    Promoter

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pDGE78

    Plasmid

    #80580

    Purpose

    recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, nptII

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Inserts

    pnos:nptII-tmos
    pPcUbi:Cas9 D10A-tnos
    BsaI-ccdB_CmR-BsaI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A

    Promoter

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pDGE79

    Plasmid

    #80581

    Purpose

    recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, BASTA

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Inserts

    pnos:PAT-tnos
    pPcUbi:Cas9 D10A-tnos
    BsaI-ccdB_CmR-BsaI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A

    Promoter

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. K222C/E334C

    Plasmid

    #24435

    Depositor

    Ron Vale

    Article

    Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

    Insert

    Kinesin (KIF5B Human)
    

    Use

    Tags

    6XHis and GFP

    Expression

    Bacterial

    Mutation

    C7S/C65A/C168A/C174S/C294A/C330S/C421A, K222C/E…

    Promoter

    Available Since

    July 13, 2010

    Availability

    Academic Institutions and Nonprofits only

37. K420CLM RP

    Plasmid

    #24443

    Depositor

    Ron Vale

    Article

    Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

    Insert

    Kinesin (KIF5B Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C7S/C65A/C168A/C174S/C294A/C330S; aa 1-420 only

    Promoter

    Available Since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

38. pCINTBeta1

    Plasmid

    #14067

    Depositor

    Richard Hynes

    Article

    Tamkun et al Cell. 1986 Jul 18. 46(2):271-82.

    Insert

    Chicken Beta 1 Integrin (ITGB1 Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    PstI-BglI fragment was blunt end cloned and EcoRI…

    Promoter

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

39. CLM RP KGF Mut 1

    Plasmid

    #24166

    Depositor

    Ron Vale

    Article

    Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

    Insert

    Kinesin (KIF5B Human)
    

    Use

    Tags

    6XHis and GFP

    Expression

    Bacterial

    Mutation

    C7S/C65A/C168A/C174S/C294A/C330S/C421A

    Promoter

    Available Since

    May 5, 2010

    Availability

    Academic Institutions and Nonprofits only

40. nlsSalicS1

    Plasmid

    #232190

    Purpose

    Novel FRET-based biosensor for salicylic acid

    Depositor

    Alexander Jones

    Article

    Tang et al Science. 2025 Oct 9;390(6769):188-194. d

    Insert

    nlsSalicS1
    

    Use

    Tags

    His-tag

    Expression

    Plant

    Mutation

    Promoter

    p16

    Available Since

    Oct. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge