We narrowed to 7,580 results for: Ski;

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W118-1_hTGM4-Flag

Plasmid

#180409

Purpose

lentiviral transduction of human TGM4 (with a C-terminal Flag tag) gene

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

TGM4 (TGM4 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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W118-1_hTGM5-V5

Plasmid

#180414

Purpose

lentiviral transduction of human TGM5 (with a C-terminal V5 tag) gene

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

TGM5 (TGM5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pWZLblasti-HA-ERK5

Plasmid

#53175

Purpose

Expresses HA tagged human ERK5 from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK5 (MAPK7 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only
pDF0250 DisCas7-11 full locus with Golden gate acceptor site for spacer

Plasmid

#172501

Purpose

Encodes the DisCas7-11 full locus along with a DR and golden gate site for cloning spacers

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

DisCas7-11 full locus with Golden gate acceptor site for spacer

Expression

Bacterial

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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BPTF PHD and Bromodomain

Plasmid

#92101

Purpose

Expresses GST fusion PHD and Bromodomain from BPTF protein

Depositor

Albert Jeltsch

Article

Mauser et al Biochimie. 2018 Mar;146:14-19. doi: 10.1

Insert

PHD and Bromodomain (BPTF Human)

Tags

GST Tag

Expression

Bacterial

Promoter

tac promoter

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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FSW-HRP-V5-NLGN2

Plasmid

#82545

Purpose

Overexpression vector for V5-NLGN2 in neurons

Depositor

Alice Ting

Article

Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

Insert

NLGN2 (Nlgn2 Rat)

Use

Lentiviral

Tags

HRP and V5

Expression

Mammalian

Promoter

Human Synapsin

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pRSET-pr-mEos3.2 (mEosFP3.2-A69T)

Plasmid

#99224

Purpose

Bacterial expression of the primed conversion capable protein pr-mEos3.2 (mEos3.2-A69T).

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mEos3.2

Expression

Bacterial

Mutation

A69T for primed conversion applications

Promoter

T7

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.dTomato.let-7c

Plasmid

#169301

Purpose

Constitutive overexpression of let-7c with dTomato as a reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

let-7c (MIRLET7C Human)

Use

Lentiviral

Tags

dTomato

Promoter

SFFV

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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GA-MFF

Plasmid

#141160

Purpose

Dimerization-dependent green fluorescent protein targeted to mitochondrial outer membrane

Depositor

Gia Voeltz

Article

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

mitochondrial fission factor (MFF Human)

Tags

GA

Expression

Mammalian

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD-eLACCO2.1

Plasmid

#208017

Purpose

Biosensor for extracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

eLACCO2.1

Expression

Bacterial

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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CathepsinG-CD4d3+4-bio

Plasmid

#73125

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding CathepsinG with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

Cathepsin G (CTSG Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA SF-HERC2 F2

Plasmid

#55785

Purpose

Expresses N-terminal 3xFLAG/STREP tagged HERC2 (950-1750aa)

Depositor

David Chan

Article

Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

Insert

3xFLAG tagged HERC2 (950aa-1750aa) (HERC2 Human)

Tags

3xFLAG and STREP

Expression

Mammalian

Promoter

CMV

Available Since

July 23, 2014

Availability

Academic Institutions and Nonprofits only
pEND-400_Cacnes_CAMP2

Plasmid

#225606

Purpose

Suicide C. acnes vector derivative of pEND-350 optimized for recombination into the CAMP2 locus, with BxbI recombination sites flanking EryR resistance cassette

Depositor

Marc Güell

Article

Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX Flag RagC S75N

Plasmid

#112757

Purpose

express Flag-RagC (S75N mutant)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-RagC (S75N) (RRAGC Human)

Use

Lentiviral

Tags

Flag

Mutation

S75N

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pSLQ10713

Plasmid

#183958

Purpose

U6-crSox2-CAG-hyperdCas12a-miniVPR

Depositor

Stanley Qi

Article

Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

Inserts

HyperdCas12a
crRNA targeting Sox2

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

D832A, D156R, E292R, D235R, D350R

Promoter

CAG and U6

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast coSHMT2

Plasmid

#106299

Purpose

Expresses SHMT2

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

serine hydroxymethyltransferase 2 (SHMT2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

Codon optimized

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-Caprola_05

Plasmid

#194666

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_05 in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_05

Tags

His-tag and Strep-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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Rescue Septin 11 mRNA

Plasmid

#122022

Purpose

Rescue mRNA for Septin 11 Knock Down with sh1 shRNA. Splice variant II (EU711415) with 5 silent mutations at the SEPT11 sh1 shRNA target site.

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Expression

Mammalian

Mutation

Silent mutations are in 5 consecutive codons (AUU…

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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U6-CjCas9-MS2gRNA-Backbone

Plasmid

#210711

Purpose

This plasmid express MS2 loop containing Cj Cas9 gRNA

Depositor

Isaac Hilton

Article

Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

Insert

MS2 loop containing Cj Cas9 gRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-Caprola_01

Plasmid

#194662

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_01 in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_01

Tags

His-tag and Strep-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pTol2_elavl3(HuC)_NES-Caprola_01-mEGFP

Plasmid

#194696

Purpose

HuC driven expression of the calcium recorder Caprola_01 fused to mEGFP for neuronal expression in zebrafish

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_01-mEGFP

Use

Zebrafish expression

Tags

mEGFP

Promoter

elavl3(HuC)

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-Caprola_13

Plasmid

#194674

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_13 in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_13

Tags

His-tag and Strep-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-cpHalo∆

Plasmid

#194659

Purpose

Protein production of the split HaloTag part cpHalo∆ (cpHaloTag135/156) in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

cpHalo∆

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-iGECI

Plasmid

#160423

Purpose

AAV plasmid for CaMKII-driven expression of a genetically-encoded calcium indicator iGECI

Depositor

Vladislav Verkhusha

Article

Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

Insert

iGECI

Use

AAV

Expression

Mammalian

Promoter

CaMKII

Available Since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-cpHaloTag141/145

Plasmid

#194660

Purpose

Protein production of circular permuted HaloTag (cpHaloTag141/145) in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

cpHaloTag141/145

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-51b(+)-cpHaloTag154/156

Plasmid

#194661

Purpose

Protein production of circular permuted HaloTag (cpHaloTag154/156) in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

cpHaloTag154/156

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS NL4-3 envFS eGFP 5'CMV Δtat ΔTARx2_d2TetOp

Plasmid

#101349

Purpose

2xTet Operator inserted between NFkB and Sp1 sites in U3 of HIV-1 delta tat with 5' CMV-R-U5. 5' and 3' TAR elements were mutated to: 5'-GGTCTCTCTGGTTAGACCAGAAAGGAGCATTGGAGCTCTCTGGCTAACTAGGGAACCC-3

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Mutation

delta tat delta TAR delta env GFP in Nef Tet ope…

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX Flag RagC W115R

Plasmid

#112759

Purpose

express Flag-RagC (W115R mutant)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-RagC (W115R) (RRAGC Human)

Use

Lentiviral

Tags

Flag

Mutation

W115R

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX Flag RagC T90N

Plasmid

#112758

Purpose

express Flag-RagC (T90N mutant)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-RagC (T90N) (RRAGC Human)

Use

Lentiviral

Tags

Flag

Mutation

T90N

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX Stat5A-SH2

Plasmid

#46515

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Stat5A (STAT5A Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 582-693

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.0_mitoLAMA-F98 IRES ShadowG-mScarlet

Plasmid

#130708

Purpose

Plasmid encoding outer mitochondrial localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and ShadowG-mScarlet

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

NTOM20-SNAP-Tag-LAMA-F98 IRES ShadowG-mScarlet

Tags

NTOM20 and SNAP-Tag

Expression

Mammalian

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pEND-193_Cacnes_HS

Plasmid

#225622

Purpose

C. acnes with heat shock inducible expression of pFAST. pBRESP36A with P(PPA_RS10245)+RBS_1+pFAST // P(PPA_RS06745)+RBS_1+HspR(PPA_RS10230)

Depositor

Marc Güell

Article

Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

Insert

HspR

Use

Synthetic Biology

Tags

pFAST-6XHis

Expression

Bacterial

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
miniABEMax V82G wt

Plasmid

#135362

Purpose

Expression of nSpyCas9 with N-terminal miniABEMax (V82G)

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

miniABEMax nSpyCas9

Use

CRISPR

Expression

Mammalian

Mutation

(V82G)

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEND-303_Cacnes_tetR

Plasmid

#225618

Purpose

C. acnes with tetR-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+TetR // P(BBa_23119-TetO2)+RBS_1+mCherry

Depositor

Marc Güell

Article

Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

Insert

TetR

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Codon optimized for Cutibacterium acnes

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEND-192_Cacnes_HS

Plasmid

#225621

Purpose

C. acnes with heat shock inducible expression of pFAST. pBRESP36A with P(PPA_RS10245)+RBS_1+pFAST // P(PPA_RS09160)+RBS_1+HspR(PPA_RS10230).

Depositor

Marc Güell

Article

Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

Insert

HspR

Use

Synthetic Biology

Tags

pFAST-6XHis

Expression

Bacterial

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHS1861

Plasmid

#205974

Purpose

FLAG-SV40 NLS-Cas8-HNH from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas8-HNH

Tags

FLAG-SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-MBP-TEVpCS-ADGRG6 CTF-FLAG

Plasmid

#217704

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGR6 (ADGRG6 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-840

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-5X1 Flag hDPY30

Plasmid

#15556

Depositor

Kai Ge

Article

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

DPY30 (DPY30 Human)

Tags

Flag and GST

Expression

Bacterial

Available Since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only
pDule2-IBBN (G2)

Plasmid

#85501

Purpose

Plasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.

Depositor

Ryan Mehl

Article

Peeler et al J Am Chem Soc. 2010 Oct 6;132(39):13575-

Insert

IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system

Expression

Bacterial

Mutation

Y32G L65E F108W Q109M D158S R162K

Promoter

lpp (constitutive)

Available Since

March 2, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX Stat5B-SH2

Plasmid

#46516

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Stat5B (STAT5B Human)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 593-692

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 7,580 results for: Ski;