We narrowed to 15,037 results for: pET

1. pET28-His10Smt3-gamma-toxin

   Plasmid

   #197000

   Purpose

   Bacterial expression plasmid for gamma toxin tRNA endonuclease

   Depositor

   Dragony Fu

   Article

   Lentini et al RNA. 2018 May;24(5):749-758. doi: 10.126

   Insert

   Gamma toxin
   

   Use

   Tags

   His10-Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-28a (+)-Gal4-vp16

   Plasmid

   #171075

   Purpose

   Expresses Gal4-vp16 fusion protein in bacteria

   Depositor

   Joan Conaway

   Article

   He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

   Insert

   Gal4 DBD (NEWENTRY Budding Yeast)
   

   Use

   Tags

   His and VP16 AD (413-490)

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-6xHis-(pos14)GFP

   Plasmid

   #89252

   Purpose

   Expresses GFP with +14 theoretical charge in E.Coli

   Depositor

   David Liu

   Article

   Thompson et al Chem Biol. 2012 Jul 27;19(7):831-43. doi

   Insert

   His6-(pos16)-GFP
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-Sac-Abeta(MC1-42)

   Plasmid

   #127151

   Purpose

   Expresses N-terminal cysteine Abeta(1-42)

   Depositor

   James Nowick

   Article

   Zhang et al Biochemistry. 2021 Apr 20;60(15):1191-12

   Insert

   Abeta(MC1-42) (APP Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET22b-T5-sfGFP*-XcP4H-MH4R

   Plasmid

   #178043

   Purpose

   Express green fluorescent protein with TAG at 151 position and enzymes to biosynthesize 5HTP

   Depositor

   Han Xiao

   Article

   Chen et al Chem. 2020 Oct 8;6(10):2717-2727. doi: 1

   Inserts

   superfolder green fluorescent protein with TAG at 151 position
   XcP4H with W179F mutation
   dihydropterine reductase from human
   pterin-4a-carbinolamine dehydratase from human
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET20b-NLuc-MBP-6xHis

   Plasmid

   #135931

   Purpose

   Expresses NanoLuciferase in bacterial cells.

   Depositor

   Antonio Amelio

   Article

   Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

   Insert

   NanoLuciferase
   

   Use

   Tags

   MBP-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-mOct4 Full length(FL)

   Plasmid

   #206394

   Purpose

   Expresses Oct4 with a 6xHis tag for protein purification

   Depositor

   Ralf Jauch

   Insert

   Oct4 (Pou5f1 Mouse)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PET-B-evoSpCas9-NLS-6xHis

   Plasmid

   #207390

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-evoSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   M495V, Y515N, K526E, R661Q, amino acids 1005-1013…

   Promoter

   T7

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET15b/Shigella flexneri IpaA

   Plasmid

   #46178

   Depositor

   Susan Craig

   Article

   Chen et al J Cell Biol. 2005 May 9;169(3):459-70.

   Insert

   IpaA
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET51b(+)_ALFA-tag-SNAP

    Plasmid

    #136628

    Purpose

    Plasmid encoding the bacterial expression of ALFA-tag asa SNAP-tag fusion

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    EGFP-NbALFA
    

    Use

    Tags

    Hisx10 and Strep

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pETM6-At4CL-CmCHS-MsCHI

    Plasmid

    #73394

    Purpose

    Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

    Depositor

    Mattheos Koffas

    Article

    Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

    Inserts

    At4CL
    CmCHS
    MsCHI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28-6His-TEV-Nsp14

    Plasmid

    #154954

    Purpose

    Expresses Nsp14 from SARS-CoV-2 with a N-terminal TEV-protease cleavable 6His tag in E. coli

    Depositor

    Justin Kollman

    Insert

    6His-TEV-Nsp14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pET-6xHis-(pos15)GFP

    Plasmid

    #89248

    Purpose

    [Edit] Expresses GFP with +15 theoretical charge in E.Coli

    Depositor

    David Liu

    Article

    Thompson et al Chem Biol. 2012 Jul 27;19(7):831-43. doi

    Insert

    His6-(pos15)-GFP
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-28a RUVBL2 Delete

    Plasmid

    #159143

    Purpose

    Expression and protien purification of a mutated RUVBL2

    Depositor

    Eric Erquan Zhang

    Article

    Ju et al Sci Transl Med. 2020 May 6;12(542). pii:

    Insert

    RUVBL2 (RUVBL2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    amino acid 2-456, Thr127-Glu233 mutated to GPPG

    Promoter

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-His6-ODC-Ctag

    Plasmid

    #163614

    Purpose

    Expresses truncated human Ornithine Decarboxylase in bacterial cells for binding to Ornithine Decarboxylase Antizyme.

    Depositor

    Mark Howarth

    Article

    Scheu et al Nat Commun. 2021 Jan 29;12(1):717. doi:

    Insert

    ODC (ODC1 Human)
    

    Use

    Tags

    C-tag and His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28-sfGFP-DogTag Loop A

    Plasmid

    #171779

    Purpose

    Expresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Val22 and Asn23

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    sfGFP-DogTag Loop A
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    DogTag and linkers inserted between residues Val2…

    Promoter

    T7

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-6xHis-(pos9)GFP

    Plasmid

    #89247

    Purpose

    Expresses GFP with +9 theoretical charge in E.Coli

    Depositor

    David Liu

    Article

    Thompson et al Chem Biol. 2012 Jul 27;19(7):831-43. doi

    Insert

    His6-(pos9)-GFP
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET-51b(+) ChemoG 3.1

    Plasmid

    #193802

    Purpose

    Protein production of ChemoG3.1 in bacteria

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoG 3.1
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    EGFP: A206K, T225R

    Promoter

    T7

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET21b-MBPx-MBPx-SpyTag003

    Plasmid

    #216291

    Purpose

    Expresses tandem MBPx linked to-SpyTag003 in bacterial cells. MBPx is a variant of MBP (A312V I317V Δ172-173 Δ175-176) with enhanced affinity for maltose.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    MBPx-MBPx-SpyTag003
    

    Use

    Affinity Reagent/ Antibody

    Tags

    His6 and SpyTag003

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pETNKI-strepII-3C-LIC-Amp

    Plasmid

    #108707

    Purpose

    Expression of a StrepII-tagged target protein with 3C protease cleavage site.

    Depositor

    Titia Sixma

    Article

    Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

    Type

    Empty backbone

    Use

    Tags

    StrepII-3C protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pETDuet-1_VNp-mNeongreen_CydAB-mCherry

    Plasmid

    #182391

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-mNeongreen and cydAB-mCherry fusions

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Inserts

    VNp-mNeongreen-His
    cydAB-mCherry
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET15b-Casp8 delta DED

    Plasmid

    #11827

    Depositor

    Guy Salvesen

    Article

    Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

    Insert

    Caspase 8 minus Death Effecter Domain (CASP8 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Minus Death Defecter Domain (aa 1-216)

    Promoter

    Available Since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

23. PET-WT SpCas9-NLS-6xHis

    Plasmid

    #207373

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    WT SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET28a-UBC9-K14R-A129K

    Plasmid

    #228948

    Purpose

    express a fragment of the double mutant of the human UBC9 (generated from pET28-UBC9 through mutagenesis; point mutations: K14R and A129K)

    Depositor

    Marcin Suskiewicz

    Insert

    UBC9 (UBE2I Human)
    

    Use

    Tags

    His tag and thrombine cleavage site : MGHHHHHHSSG…

    Expression

    Bacterial

    Mutation

    changed Lysine 14 to Arginine, changed Alanine 12…

    Promoter

    T7-lac

    Available Since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28-T7-NlaCas3-NLS-6xHis

    Plasmid

    #180214

    Purpose

    Expressing Neisseria lactamica Type I-C Cas3 with NLS and His tag on the C terminus for protein purification in E.Coli

    Depositor

    Yan Zhang

    Article

    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

    Insert

    NlaCas3c
    

    Use

    Tags

    6xHis and NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET-45b-mCherry-Cbx5

    Plasmid

    #194547

    Purpose

    Bacterial Expression of 6xHis mCherry-HP1a Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    Cbx9 (HP1a)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET19b-EcMscL-TEV-mEGFP

    Plasmid

    #165097

    Purpose

    Expresses EcMscLGFP as a fusion protein in E. coli BL21or using in vitro expression.

    Depositor

    Neha Kamat

    Article

    Jacobs et al Proc Natl Acad Sci U S A. 2019 Feb 13. p

    Insert

    E. Coli Mechanosensitive Channel of Large Conductance (mscL E. coli)
    

    Use

    Synthetic Biology

    Tags

    monomeric Enhanced Green Fluorescent Protein

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET28-MHL: nsp16_SARS-CoV-2

    Plasmid

    #167850

    Purpose

    Bacterial expression of the SARS-CoV-2 protein nsp16

    Depositor

    Cheryl Arrowsmith

    Article

    Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

    Insert

    nsp16
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 16, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pET28-MHL: nsp10_SARS-CoV-2

    Plasmid

    #167851

    Purpose

    Bacterial expression of the SARS-CoV-2 protein nsp10

    Depositor

    Cheryl Arrowsmith

    Article

    Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

    Insert

    nsp10
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 16, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pET28a-SENP7 (catalytic domain)

    Plasmid

    #16360

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    human SENP7 catalytic domain (SENP7 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Amino Acid 640 to 984 catalytic domain of human S…

    Promoter

    Available Since

    Dec. 19, 2007

    Availability

    Academic Institutions and Nonprofits only