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pCFJ2059 - [1-2] ENTR - Tag - Halo (intron(1), syntrons(3), no_atg, no_stop)

Plasmid

#159872

Purpose

Three-fragment Gateway compatible protein tag for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Tag - Halo (intron(1), syntrons(3), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pSEM246 - MCS mosTI unc-119 target

Plasmid

#159821

Purpose

Single copy or array insertion by MosTI using split unc-119 selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

MCS mosTI unc-119 target

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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mTFP1-LaminB1-7

Plasmid

#55492

Purpose

Localization: Nuclear Envelope, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

LaminB1 (LMNB1 Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
mTFP1-EB3

Plasmid

#55477

Purpose

Localization: MT End Binding Protein, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

EB3 (MAPRE3 Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
pmiRFP-H2B-C1

Plasmid

#108411

Purpose

Histone 2B fused to monomeric near-infrared fluorescent protein miRFP

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

miRFP-H2B (H2BC21 Human)

Tags

miRFP

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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Str-KDEL-SBP-EGFP-LAMP1

Plasmid

#222317

Purpose

Synchronize the trafficking of LAMP1 from the ER.

Depositor

Franck Perez

Article

Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

Insert

Streptavidin-KDEL and LAMP1 fused to SBP-EGFP (LAMP1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pKb-TnpB1

Plasmid

#215333

Purpose

ISDra2 expression driven by PolII promoter ; 20 bp guide RNA can be cloned in BsaI site

Depositor

Kutubuddin Molla

Article

Karmakar et al Plant Biotechnol J. 2024 Jun 28. doi: 10

Insert

Rice codon optimized ISDra2 TnpB

Tags

Not applicable

Expression

Plant

Promoter

Rice ubiquitin promoter for TnpB and OsU3 promote…

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pMXs-hSOX2-17

Plasmid

#193350

Purpose

Retroviral vector expressing human SOX2-17 chimeric transcription factor (super-SOX)

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

SOX2-17 (SOX2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

SOX2-17 is engineered highly cooperative chimeric…

Promoter

5'LTR

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 N-term COILIN

Plasmid

#187581

Purpose

Express FLAG epitope and APEX2-tagged COILIN fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

COILIN (COIL Human)

Tags

APEX2-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pRSET-pr-mKikGR1 (mKikGR1-V69T)

Plasmid

#99227

Purpose

Bacterial expression of the primed conversion capable protein pr-mKikGR1 (mKikGR1-V69T).

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mKikGR1

Expression

Bacterial

Mutation

monomeric variant with mutation V69T (Eos notatio…

Promoter

T7

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pBABE Zeo IkB SR

Plasmid

#83023

Purpose

Retroviral expression of dominant negative IkB alpha

Depositor

Yi Zhang

Article

Kim et al Cell Rep. 2016 Sep 27;17(1):275-288. doi

Insert

NFKB Inhibitor Alpha (NFKBIA Human)

Use

Retroviral

Mutation

Serine 32 to Alanine, Serine 36 to Alanine

Available Since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α1.1-Archon1-KGC-GFP-ER2

Plasmid

#115890

Purpose

AAV-mediated expression of Archon1-KGC-GFP-ER2 under the EF1α promoter (1.1kb short version). Using SV40 signal.

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

Archon1-KGC-GFP-ER2

Use

AAV

Tags

ER2, GFP, and KGC

Expression

Mammalian

Promoter

EF1a（1.1kb short version)

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc NES-mCE NLS

Plasmid

#82466

Purpose

Expresses cytoplasmic restricted form of myc tagged mRNA capping enzyme

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

NES mRNA capping enzyme (Rngtt HIV, Mouse)

Tags

Myc

Expression

Mammalian

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV3TAG8li-hSTT3A-3FLAG

Plasmid

#159140

Purpose

Hu STT3A -flag mammalian expression vector.

Depositor

Martin Dorf

Article

Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

Insert

STT3 oligosaccharyltransferase complex catalytic subunit A (STT3A Human)

Tags

3x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 5, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-Myo 1d

Plasmid

#134906

Purpose

Expression of rat Myo 1d (Myr 4) in mammalian cells.

Depositor

Martin Bähler

Article

Bahler et al J Cell Biol. 1994 Jul;126(2):375-89. doi

Insert

Myo 1d (rat) with 3' UTR (Myo1d Rat)

Tags

EGFP

Expression

Mammalian

Promoter

CMV IE

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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anti-Desmin_D7-pBIOCAM5

Plasmid

#39346

Depositor

John McCafferty

Article

Schofield et al Genome Biol. 2007;8(11):R254.

Insert

scFv-Fc-fusion specific for Desmin (clone D7) (DES Human)

Tags

3xFLAG, 6xHis, and human Fc

Expression

Mammalian

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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CIB-VP16 (B1014)

Plasmid

#92036

Purpose

Expresses CIB1 (full length, with endogenous NLS) fused to VP16 activation domain.

Depositor

Chandra Tucker

Article

Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

Insert

CIB1 (CIB1 Mustard Weed)

Tags

Fusion of plant CIB1 (intact NLS) with VP16 activ…

Expression

Mammalian

Promoter

T7

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1X-Nup93-mut

Plasmid

#87335

Purpose

To express human Nup93 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP93 (NUP93 Human)

Tags

EGFP

Expression

Mammalian

Mutation

shRNA resistant changes

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pMDJ23 - pEXP[Psmu-2 | eFlp recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

Plasmid

#159815

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Psmu-2 | eFlp recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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GST PTBP1 isoform 4

Plasmid

#108594

Purpose

Recombinant GST tagged protein production

Depositor

Chris Smith

Article

Coelho et al EMBO J. 2015 Mar 4;34(5):653-68. doi: 10

Insert

PTBP1 isoform 4 (PTBP1 Human)

Tags

GST

Expression

Bacterial

Mutation

isoform 4 (isoform a)

Promoter

tac

Available Since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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