We narrowed to 165,567 results for: addgene

1. DNA-PKcs N-terminal sgRNA

   Plasmid

   #207093

   Purpose

   pX330 based plasmid for expression of Cas9 and the AGCGGGACTCGGCGGCATGG sgRNA to target the DNA-PKcs locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   AGCGGGACTCGGCGGCATGG
   

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. 7TG

   Plasmid

   #24314

   Depositor

   Roel Nusse

   Article

   Brugmann et al Development. 2007 Sep . 134(18):3283-95.

   Insert

   7xTcf-eGFP
   

   Use

   Lentiviral

   Mutation

   EGFP is GFP with S65T and F64L mutations

   Available Since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLentiCRISPRv2-Stuffer-HepmCherry

   Plasmid

   #192825

   Purpose

   Contains stuffer sequence into which sgRNAs can be cloned and expresses mCherry from hepatocyte-specific promoter

   Depositor

   Kristin Knouse

   Article

   Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

   Insert

   mCherry
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   Hepatocyte-specific promoter (HS-CRM8-TTRmin modu…

   Available Since

   Nov. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti UBC hNLRP3-mNG

   Plasmid

   #218642

   Purpose

   Expresses NLRP3 tagged with mNeonGreen in mammalian cells (lentiviral); driven by the weak UBC promoter with puro selection

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   NLRP3 (NLRP3 Human)
   

   Use

   Lentiviral

   Tags

   mNeonGreen

   Expression

   Mammalian

   Promoter

   UBC

   Available Since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. ULK1 sgRNA

   Plasmid

   #207559

   Purpose

   pX330 expressing Cas9 and a sgRNA targeting the ULK1 locus

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   CCAGCCAGGCCAGAAAGGTC
   

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVPT-rtTR-KRAB

   Plasmid

   #11777

   Purpose

   Tet-regulated (Tet-off) lentiviral vector for transgene (mPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

   Depositor

   Patrick Aebischer, Didier Trono

   Article

   Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

   Insert

   mPGK, GFP, rtTR-KRAB, Tet-off
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSNAPf-Nup43

   Plasmid

   #98276

   Purpose

   mammalian expression of SNAPf-Nup43

   Depositor

   Mike Heilemann

   Article

   Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

   Insert

   Nup43 (NUP43 Human)
   

   Tags

   SNAPf-tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. 6BHD (SETDB1)

   Plasmid

   #119955

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   SETDB1 (SETDB1 Human)
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   contains amino acids 190-410

   Promoter

   T7

   Available Since

   Feb. 1, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. ATG9A sgRNA

   Plasmid

   #207557

   Purpose

   pX330 expressing Cas9 and a sgRNA targeting the ATG9A locus

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   CACTGAATACCAGCGCCTAG
   

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. MXS_CMV::PuroR-bGHpA

    Plasmid

    #62439

    Purpose

    MXS_chaining vector with CMV::PuroR-bGHpA

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    resistance cassette against Puromycin with CMV Promoter
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. WIPI2 sgRNA

    Plasmid

    #207554

    Purpose

    pX330 expressing Cas9 and a sgRNA targeting the WIPI2 locus

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    CGCGCGCCCAGCCATGAACC
    

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNR64

    Plasmid

    #79540

    Purpose

    Input plasmid: expresses two recombinases downstream of two inducible promoters. Same as Dual-Recombinase-Controller (plasmid #44456) from Endy Lab except with KanR instead of CamR on the backbone.

    Depositor

    Timothy Lu

    Article

    Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

    Inserts

    TP901
    BxbI
    

    Tags

    His tag and LAA degradation tag

    Expression

    Bacterial

    Available Since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pL1P5 ZmUbiP:GRF-GIF:NosT

    Plasmid

    #198046

    Purpose

    MoClo Golden Gate Level 1 Position 5. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.

    Depositor

    John Innes Centre - Wheat Transformation and Genome Editing, Cristobal Uauy

    Insert

    ZmUbiP::TaGRF4-GIF1::NosT
    

    Use

    Synthetic Biology

    Promoter

    Zea mays (maize) ubiquitin promoter

    Available Since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIgnaviCas9

    Plasmid

    #127595

    Purpose

    Expresses IgnaviCas9 in E. coli

    Depositor

    Stephen Quake

    Article

    Schmidt et al Proc Natl Acad Sci U S A. 2019 Oct 28. p

    Insert

    IgnaviCas9
    

    Tags

    6xHis Tag and Maltose binding protein

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHPyV7-713a

    Plasmid

    #24728

    Depositor

    Christopher Buck

    Article

    Schowalter et al Cell Host Microbe. 2010 Jun 25;7(6):509-

    Insert

    Full genome of Human polyomavirus 7
    

    Use

    Viral clone

    Available Since

    June 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. ICP8-P2A/Crimson (pSLIK4)

    Plasmid

    #113859

    Purpose

    For Doxycycline-inducible expression of ICP8 synaptase gene, with ICP8-P2A-red fluorescent protein gene E2-Crimson

    Depositor

    Richard S Myers

    Article

    Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

    Insert

    ICP8
    

    Use

    Lentiviral

    Tags

    P2A/Crimson

    Available Since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. p8389 LentiCRISPRv2 Neo sgNT-1

    Plasmid

    #221649

    Purpose

    Expression of non-targeting control sgRNA

    Depositor

    Elizabeth White

    Article

    Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

    Insert

    non-targeting sgNT-1
    

    Use

    CRISPR and Lentiviral

    Available Since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBEVY-T

    Plasmid

    #51232

    Purpose

    bi-directional expression vector for yeast, constitutively active, TRP1 selection

    Depositor

    Charles Miller

    Article

    Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

    Type

    Empty backbone

    Expression

    Yeast

    Promoter

    GPD/ADH1

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHPyV6-607a

    Plasmid

    #24727

    Depositor

    Christopher Buck

    Article

    Schowalter et al Cell Host Microbe. 2010 Jun 25;7(6):509-

    Insert

    Full genome of Human polyomavirus 6
    

    Use

    Viral clone

    Available Since

    June 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDB4280

    Plasmid

    #98699

    Purpose

    A Cas9-encoding plasmid containing the 5' portion of the ura4 marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-ura4 system

    Depositor

    Li-Lin Du

    Article

    Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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