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Plasmid#188520PurposeBackbone for CRISPR interference (CRISPRi) isogenic PAM testingDepositorTypeEmpty backboneUseLentiviralTagseGFPExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only
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pEGFP-hsRPA4 (MSW#517)
Plasmid#208070PurposeExpression of GFP-tagged hsRPA4 in human cellsDepositorInsertRPA4 (RPA4 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-EGFP-ChIEF
Plasmid#172371PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInsertEGFP + ChIEF
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV2_hSyn_NES-Caprola_04-mEGFP_WRPE-SV40
Plasmid#194688PurposehSyn1 driven expression of the calcium recorder Caprola_04 fused to mEGFP for neuronal expression through AAV transductionDepositorInsertCaprola_04-mEGFP
UseAAVTagsmEGFPExpressionMutationPromoterhSyn1Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-nl.EGFP-FlpO
Plasmid#172379PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInsertnuclear-localized EGFP + FlpO
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-SYN-eGFP-RAB11
Plasmid#203731PurposeAAV vector plasmid expressing human RAB11 fused to eGFP under the human synapsin (SYN) promoterDepositorInsertRAB11 (RAB11A Human)
UseAAVTagseGFPExpressionMammalianMutationPromoterHuman synapsin promoterAvailable sinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF6V5:eGFP-CAAX-2A-mCherry
Plasmid#26901DepositorInserteGFP-CAAX-PTVI-2A-mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HtrA2-EGFP S306A
Plasmid#14122DepositorInsertHtrA2 (HTRA2 Human)
UseTagsEGFPExpressionMammalianMutationS306APromoterAvailable sinceMarch 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-RIαΔ(D/D+linker)-EGFP
Plasmid#181843PurposeGFP-tagged PKA RIα subunit lacking the docking/dimerization (D/D) and linker regions; for mammalian expression.DepositorInsertRIα-(D/D+linker)EGFP (PRKAR1A Human)
UseTagsEGFPExpressionMammalianMutationDeletion of residues 12-112 in PKA RIα.PromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-uTEV1-MAVS-mTA4
Plasmid#171002PurposeHiLITR protease with MAVS [Y536delinsFIVLI] C-terminal tmd targeting information (Mitochondria and ER insertion)DepositorInsertEGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI]
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationMAVS(tmd)[Y536delinsFIVLI]PromoterpTRE-tight (TetOn)Available sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;dll4CE1-P1Egfp
Plasmid#90146Purposecontains dll4 enhancer 1 sequence, and dll4 specific gene promoter driving expression of eGFPDepositorInsertdll4E1P1Egfp (dll4 Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
Hy_pUbi-p63e eGFP SV40
Plasmid#132650PurposeExpresses eGFP mRNA from the Drosophila Ubi-p63e promoterDepositorInserteGFP
UseTagsExpressionInsectMutationPromoterUbi-p63eAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHDR-eGFP (pXK-1647)
Plasmid#208824PurposeThe HDR-based surrogate reporter for validating HDR-editing activity of different gene editing tools as well as for helping to screen HDR-editing positive cells. Key Construct: CAG-PuroR-CMV-eGFP*(HDRDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTox-EGFPsite1_NGG-PAM (BPK740)
Plasmid#181746Purposep11-lacY-wtx1 derivative toxic plasmid encoding an SpCas9 target site for EGFP site 1 with an NGG PAMDepositorInsertccdB toxin gene and SpCas9 target site (EGFP site 1 NGG PAM)
UseSelection plasmidTagsExpressionMutationPromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-EGFP-2A-HA-Nrxn3-alpha- 2(-)/3(+)/4(+)/6(+)
Plasmid#184334PurposeExpression of mouse Nrx3-alpha lacking insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.DepositorInsertNrxn3-alpha (Nrxn3 Mouse)
UseTagsHA-tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
[UBC][NRE Pum][eGFP]
Plasmid#97192PurposeThe wild-type Pum, fused to eGFP, and behind the ubiquitin promoter.DepositorInsertwild type Pumilio homology domain
UseSynthetic BiologyTagseGFPExpressionMammalianMutationPromoterUBC (ubiquitin C)Available sinceNov. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
Actin-53b-ires-EGFP
Plasmid#25459DepositorInsertBAF53b (Actl6b Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET23-CBD-(-PP)-EGFP-H246,249D
Plasmid#12598DepositorTypeEmpty backboneUseTags6xHis and EGFPExpressionBacterialMutationPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-FOXF1-MUT
Plasmid#194569PurposeMammalian Expression of mEGFP-FOXF1 Mutant (A231Rfs*61) Fusion ProteinDepositorInsertFOXF1 (FOXF1 Human)
UseTagsmEGFPExpressionMammalianMutationA231Rfs*61PromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;tmem88aCE1-P1Egfp
Plasmid#90148Purposecontains tmem88a enhancer sequence, and tmem88a specific gene promoter driving expression of eGFPDepositorInserttmem88aE1P1Egfp (tmem88a Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
CRY2clust-eGFP-2xC2-iLID
Plasmid#114420Purposeanchor iLID domain to plasma membrane with slower diffusionDepositorInsertiLID domain
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;lmo2CE1-P1Egfp
Plasmid#90153Purposecontains lmo2 enhancer sequence, and lmo2 specific gene promoter driving expression of eGFPDepositorInsertlmo2E1P1Egfp (lmo2 Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCK072 p5E 147bp exorh:EGFP
Plasmid#195950PurposeGateway p5E vector with reporterDepositorInsertexorh minimal regulatory elements and EGFP
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP Rab11a WT pcDNA3.1 Zeo (+)
Plasmid#206079PurposeRab11a belongs to the Rab family of small GTPases, Rab11 proteins are particularly involved in trafficking through recycling endosomesDepositorInsertRAB11A (RAB11A Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDest-1.7kbfabp6-eGFP-pA-CrymCherry
Plasmid#159088PurposeLR construct using backbone with Cry:mCherry insert to identify fish with transgene, and with p5E-1.7kbfabp6, pME-eGFP, and p3E-pA insertsDepositorInsertsUseFluorescent reporterTagsExpressionMutationPromoter1.7kb fabp6Available sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
p152 pWZL-EGFP RanBP10
Plasmid#11098DepositorInsertRanBP10 (Ranbp10 Mouse)
UseRetroviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2005AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso1_1093-1317_W
Plasmid#147892PurposeMammalian Expression of HsNot1iso1_1093-1317 *Please note that this plasmid encodes amino acids 1093-1322 (not 1093-1317 as the name suggests).DepositorInsertHsNot1iso1_1093-1317 (CNOT1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso1_1318-1605_W
Plasmid#147893PurposeMammalian Expression of HsNot1iso1_1318-1605DepositorInsertHsNot1iso1_1318-1605 (CNOT1 Human)
UseTagsExpressionMammalianMutation1 silent mutation compared to the sequence of iso…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIHEU_miniSOG2 T2A H3.3A-EGFP
Plasmid#87411PurposeUAS vector to express Histone 3.3A GFP fusion and miniSOG2 in DrosophilaDepositorInsertmini singlet oxygen generator 2
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-RNF8-N-terminus(1-111)
Plasmid#64671PurposeExpresses only N terminus of RNF8, amino acids 1 to 111DepositorInsertring finger protein 8 (RNF8 Human)
UseTagsGFPExpressionMammalianMutationcontaining only N-terminus amino acids 1 to 111PromoterCMVAvailable sinceMay 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQL47 EGFP-VAMP-GST
Plasmid#122971PurposeReporter of botulinum toxin B cleavageDepositorInsertEGFP-VAMP3-GST
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Fuse-gcry-eGFP
Plasmid#117785PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertmRFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-puro-eGFP-HEC1-9A
Plasmid#114023PurposeInducible expression of eGFP-tagged HEC1-9A in FLPin Trex cell linesDepositorInserteGFP-HEC1 - 9A (NDC80) (NDC80 Human)
UseTagsExpressionMammalianMutationHec1 - 9APromoterAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
Pet22b-mEGFP-YAP-N-IDR
Plasmid#166440PurposeBacterial expression of 6x His tagged mEGFP-YAP1-N-IDR fusion proteinDepositorInsertmEGFP, YAP-N-IDR(1-164) (YAP1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_shRNA resistant PKA-Cα-mEGFP
Plasmid#114143PurposeMammalian expression of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.DepositorInsertmouse PKA-Cα-mEGFP with silent mutations resisting shRNA knockdown
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-HSD17B11-V16D-FLAGC
Plasmid#184500PurposeExpresses the V16D mutant of human HSD17B11 with a C-terminal FLAG-GFP tag. Confers G418 resistance.DepositorInsert17-beta-hydroxysteroid dehydrogenase 11 (HSD17B11 Human)
UseTagsFLAGC-GFPExpressionMammalianMutationV16DPromoterCMVAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-HSD17B11-L14P-FLAGC
Plasmid#184499PurposeExpresses the L14P mutant of human HSD17B11 with a C-terminal FLAG-GFP tag. Confers G418 resistance.DepositorInsert17-beta-hydroxysteroid dehydrogenase 11 (HSD17B11 Human)
UseTagsFLAGC-GFPExpressionMammalianMutationL14PPromoterCMVAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-HSD17B11-S172L-FLAGC
Plasmid#161904PurposeExpresses the catalytically inactive S172L mutant of human HSD17B11 with a C-terminal FLAG-GFP tag. Confers G418 resistance.DepositorInserthydroxysteroid 17-beta dehydrogenase 11 (HSD17B11 Human)
UseTagsFLAG-GFPExpressionMammalianMutationS172L; catalytically dead mutantPromoterCMVAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-KSENB-Nup133-mut
Plasmid#87339PurposeTo express human Nup133 in mammalian cells. It contains synonymous mutations that are not targeted by shRNADepositorInsertNUP133 (NUP133 Human)
UseTagsEGFPExpressionMammalianMutationshRNA resistant changes; deletion of Nup133 AA1-7PromoterCMVAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-TagRFP-cmlc2-eGFP
Plasmid#117770PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertTagRFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only