We narrowed to 29,109 results for: PLE;

1. pLenti-U6-sgCTG-CAG-Cas9D10A nickase-Blast

   Plasmid

   #216733

   Purpose

   Expresses SpCas9-D10A nickase under a CAG promoter together with a blasticidin resistance gene, along with a U6 promoter driving the sgCTG (target sequence: (CUG)6). Suitable for lentivirus packaging.

   Depositor

   Vincent Dion

   Article

   Alvaro et al bioRxiv

   Insert

   Cas9-D10A nickase and sgCTG
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   U6 and CAG

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. plenti-UbcP-IKZF3-3xHA-pGK-Pur

   Plasmid

   #69053

   Purpose

   lentiviral expression of IKZF3 with HA tag

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   IKZF3 (IKZF3 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-CAG-NOTCH2NLB (N2NL-FL)-ires-EGFP

   Plasmid

   #122954

   Purpose

   Lentiviral expression of NOTCH2NLB full length under CAG promoter with EGFP expression

   Depositor

   Pierre Vanderhaeghen

   Article

   Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

   Insert

   NOTCH2NLB (NOTCH2NLB Human)
   

   Use

   Lentiviral

   Tags

   6xhis tag

   Expression

   Mutation

   Promoter

   CAG promoter

   Available since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-CD74-ROS1 S1986F/G2032R/L2086F

   Plasmid

   #183822

   Purpose

   Express CD74-ROS1 fusion (C6;R34) harboring ROS1 S1986F/G2032R/L2086F mutation in mammalian cells

   Depositor

   Aaron Hata

   Article

   Lin et al Clin Cancer Res. 2021 May 15;27(10):2899

   Inserts

   ROS Proto-Oncogene 1, Receptor Tyrosine Kinase (ROS1 Human)
   CD74 molecule (CD74 Human)
   

   Use

   Lentiviral

   Tags

   The fusion protein of CD74 exon 1-6 and ROS1 exon…

   Expression

   Mammalian

   Mutation

   S1986F/G2032R/L2086F

   Promoter

   CMV

   Available since

   April 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti NLuc398-hLGALS8 IRES hCALCOCO2-CLuc394

   Plasmid

   #128387

   Purpose

   Expresses a split firefly luciferase reporter based on the full length, human Gal8 and CALCOCO2, which interact following endosomal disruption

   Depositor

   Craig Duvall

   Article

   Kilchrist et al ACS Sens. 2020 Jun 23. doi: 10.1021/acss

   Inserts

   NLuc398-3xGGGGS-LGALS8 (LGALS8 Synthetic, Human)
   CALCOCO2-3xGGGGS-CLuc394 (CALCOCO2 Human)
   

   Use

   Lentiviral, Luciferase, and Synthetic Biology

   Tags

   CLuc394 and NLuc398

   Expression

   Mammalian

   Mutation

   Promoter

   EFS and IRES

   Available since

   Jan. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. hu-TLR4 299 SNP flag pLenti 4/to/v5 pDEST

   Plasmid

   #42650

   Purpose

   destination vector for lentivirus-mediated expression of human TLR4 299 SNP in mammalian cells

   Depositor

   Scott Friedman

   Article

   Guo et al Hepatology. 2009 Mar;49(3):960-8. doi: 1

   Insert

   TLR4 299 SNP (TLR4 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   D299G

   Promoter

   CMV/TO

   Available since

   March 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-EF1alpha-IRES-EGFP-Flag-hRPGRIP1L

   Plasmid

   #122991

   Purpose

   Expresses Flag-tagged hRPGRIP1L in mammalian cells

   Depositor

   Ken-Ichi Takemaru, FENG-QIAN Li

   Insert

   RPGRIP1L (RPGRIP1L Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mutation

   Promoter

   EF1 alpha

   Available since

   March 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-pGK-nAChRb2(E61C)-IRES-GFP

   Plasmid

   #164777

   Purpose

   cysteine mutated nAChR beta2 subunit (E61C) for the attachment of a photoswitchable tethered ligand

   Depositor

   Alexandre Mourot

   Article

   Durand-de Cuttoli et al Elife. 2018 Sep 4;7. pii: 37487. doi: 10

   Insert

   nAChR b2 E61C - IRES- GFP (Chrnb2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   E61C

   Promoter

   pGK

   Available since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti CMV-MRLC1-mRuby2-IRES-PuroR

   Plasmid

   #103031

   Purpose

   Lentiviral expression of mRuby2-tagged MRLC with puromycin selection

   Depositor

   Ghassan Mouneimne

   Article

   Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

   Insert

   MRLC1 (MYL9 Human)
   

   Use

   Lentiviral

   Tags

   mRuby2

   Expression

   Mutation

   Promoter

   CMV immediate early

   Available since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti PGK mKate2-OGT 5N5A (Puro)

    Plasmid

    #154293

    Purpose

    Lentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, 5N5A variant, no HCF-1 cleavage)

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Lentiviral

    Tags

    2x FLAG and mKate2

    Expression

    Mammalian

    Mutation

    N322A, N356A, N390A, N424A, N458A mutations; HCF-…

    Promoter

    PGK

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLEX-uORF-HA-birA*-STOP-IRES-Puro

    Plasmid

    #120558

    Purpose

    Expresses control HA-birA* in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    birA
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    R118G

    Promoter

    CMV

    Available since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. plenti-CAG-IKZF2-V2-FLAG-IRES-GFP

    Plasmid

    #69049

    Purpose

    lentiviral expression of IKZF2 variant 2 with FLAG tag

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    IKZF2 (IKZF2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and IRES-GFP

    Expression

    Mammalian

    Mutation

    splice variant 2

    Promoter

    CAG

    Available since

    Oct. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-EF1a-SPdCas9-DNMT3A(E752A)-2A-Blast

    Plasmid

    #71218

    Purpose

    dCas9 fused to the human DNMT3A catalytically inactive domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-DNMT3A(E752A)
    

    Use

    Lentiviral

    Tags

    SPdCas9-DNMT3A(E752A)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-EF1a-CDK6-mutPAM-PGK-puro

    Plasmid

    #187638

    Purpose

    sg resistant CDK6 ORF

    Depositor

    William Kaelin

    Article

    Nicholson et al Sci Signal. 2019 Oct 1;12(601). pii: 12/

    Insert

    CDK6 (CDK6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    PAM site mutation

    Promoter

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. plenti-UbcP-Myc-CRBN-pGK-HYG

    Plasmid

    #107376

    Purpose

    Lentiviral expression of myc-CRBN

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    cereblon (CRBN Human)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    UbcP

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-TRS-Leader-SARS-CoV-2-EGFP

    Plasmid

    #171585

    Purpose

    Expression of 75 nt of the SARS-CoV-2 Leader sequence at 5' of destabilized eGFP reporter

    Depositor

    Neville Sanjana

    Article

    Mendez-Mancilla et al Cell Chem Biol. 2022 Feb 17;29(2):321-32

    Insert

    TRS-Leader-SARS-CoV-2-d2eGFP
    

    Use

    Lentiviral

    Tags

    2PEST AND NLS

    Expression

    Mutation

    Promoter

    Available since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. Triple ORF LoxP ifgMosaic Donor (LH208)

    Plasmid

    #99625

    Purpose

    To clone the 3 ORFs for Cre dependent generation of ifgMosaics

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    N-PhiM
    

    Use

    Cre/Lox, Mouse Targeting, and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. plenti-UbcP-IKZF1-V2-3xHA-pGK-Pur

    Plasmid

    #69051

    Purpose

    lentiviral expression of IKZF1 with HA tag

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    IKZF1 (IKZF1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    splice variant 2

    Promoter

    UBC

    Available since

    Nov. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti CMV Puro DEST (w118-1) MXRA7

    Plasmid

    #107500

    Purpose

    Expresses MXRA7, puromycin resistant

    Depositor

    Alejandro Gutierrez

    Article

    Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

    Insert

    MXRA7 (codon optimized) (MXRA7 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Codon optimized sequence

    Promoter

    CMV

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-PGK-flag-RAP1B-V12-puro

    Plasmid

    #156168

    Purpose

    Lentiviral vector for expression of flag-RAP1B-V12, with puromycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP1B (RAP1B Bovine)
    

    Use

    Lentiviral

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    changed Glycine 12 to Valine

    Promoter

    human PGK

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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