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pXPR_501

Plasmid

#96922

Purpose

for CRISPRa, lentiviral expression of gRNA scaffold using activator VP64

Depositor

John Doench, David Root

Article

Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pMIG-FLAG-MLL-AF9

Plasmid

#71443

Purpose

Retroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-GFP

Depositor

Daisuke Nakada

Article

Saito et al Cell Stem Cell. 2015 Sep 29. pii: S1934-

Insert

MLL-Af9 (KMT2A Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

MSCV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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Lenti-EF1alpha-VP64-dCas9-VP64_Blast

Plasmid

#192650

Purpose

3rd generation lenti vector encoding VP64-dCas9-VP64 with 2A Blast resistance marker

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

VP64-dCas9-VP64

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A and N863A in Cas9

Promoter

EF1a

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K48

Plasmid

#17605

Purpose

Mammalian expression of HA tagged ubiquitin with only K48, other lysines mutated to arginines

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K48 only, other lysines mutated to arginines. Enh…

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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pAF0692 Ef1a-Dn29_attD-Puro-p2a-mCherry

Plasmid

#193465

Purpose

Dn29 Donor with promoter for mcherry and puro expression

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Dn29_attP

Expression

Mammalian

Promoter

Ef1a

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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STAT3-GFP

Plasmid

#110495

Purpose

reporter of STAT3 signaling activity

Depositor

Michael Lewis

Article

Wei et al Stem Cells. 2014 Oct;32(10):2571-82. doi

Insert

4 repeats of STAT3 binding site M67 at GFP promoter region

Use

Lentiviral

Expression

Mammalian

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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PB-CRISPR

Plasmid

#160047

Purpose

For cloning sgRNA to knockout a specific gene in piggybac plasmid

Depositor

Xiaojun Lian

Article

Jiang et al Bioact Mater. 2022 Feb 7;14:313-320. doi

Type

Empty backbone

Use

CRISPR; Piggybac

Expression

Mammalian

Promoter

EFS

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pQUAST-attB

Plasmid

#104880

Purpose

For insertion of 5xQUAS-geneX transgenes using PhiC31 integrase system

Depositor

Christopher Potter

Type

Empty backbone

Expression

Insect

Available Since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

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NOVA1 eGFP

Plasmid

#61275

Purpose

GFP-tagged ORF clone of Homo sapiens neuro-oncological ventral antigen 1 (NOVA1), transcript variant 1

Depositor

Nicolas Charlet-Berguerand

Insert

NOVA1 (NOVA1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pCXLE-mCherry

Plasmid

#193293

Purpose

episomal vector expressing mCherry fluorescent protein

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

mCherry

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3-Flag MKK6(glu)

Plasmid

#13518

Depositor

Roger Davis

Article

Raingeaud et al Mol Cell Biol. 1996 Mar . 16(3):1247-55.

Insert

MKK6(glu) (MAP2K6 Human)

Tags

Flag

Expression

Mammalian

Mutation

Ser-207Glu Thr-211Glu

Promoter

CMV

Available Since

July 25, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA-DDX11-Flag-WT

Plasmid

#120727

Purpose

Expression of a Flag-tagged version of DDX11 WT in mammalian cells

Depositor

Francesca Pisani

Article

Cortone et al PLoS Genet. 2018 Oct 10;14(10):e1007622.

Insert

DDX11 (DDX11 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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GST-PS-Grb2 (XSB363)

Plasmid

#78505

Purpose

Construct for expressing Grb2 in E. Coli

Depositor

Ron Vale

Article

Su et al Science. 2016 Apr 29;352(6285):595-9. do

Insert

Grb2 (GRB2 Human)

Tags

GST followed by a prescission protease claevage s…

Expression

Bacterial

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCK017 pDEST exorh:EGFP

Plasmid

#195983

Purpose

Transgenesis backbone

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Other

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Puro-dTAG

Plasmid

#207694

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-dTAG cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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p5E-2.3k-fabp2

Plasmid

#84927

Purpose

–2.3kb promoter of zebrafish fabp2/ifabp gene in Gateway 5’ Entry vector

Depositor

John Rawls

Insert

–2.3kb promoter of zebrafish fabp2/ifabp gene (fabp2 Zebrafish)

Tags

NO

Expression

Bacterial

Available Since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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p7056 pHAGE-P-CMVt-N-HA-GAW-CPT1A

Plasmid

#100146

Purpose

Expresses CPT1A

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

CPT1A (CPT1A Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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PRPF4

Plasmid

#155850

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

PRPF4 (PRPF4 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCherry.90 alpha

Plasmid

#108222

Purpose

Mammalian expression vector for mCherry fusion to human Hsp90 alpha

Depositor

Didier Picard

Article

Lev et al Immunity. 2008 Jun;28(6):787-98. doi: 10

Insert

mCherry-Hsp90a (HSP90AA1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pBS130

Plasmid

#26290

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

phiC31 integrase (int Streptomyces phage phiC31)

Use

Drosophila transgenesis

Expression

Bacterial and Insect

Available Since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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