We narrowed to 13,583 results for: TIM

1. pSW002-Pc-mRuby2

   Plasmid

   #111250

   Purpose

   Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with mRuby2 (codon optimized for P. fluorescens)

   Depositor

   Rosemarie Wilton

   Article

   Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

   Insert

   mRuby2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   mRuby2 is codon optimized for expression in P. fl…

   Promoter

   Pc

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Intron-Tagging-EGFP-Donor

   Plasmid

   #159740

   Purpose

   Contains EGFP flanked by a splice acceptor and a splice donor. Together with other intron tagging plasmids, it can be used to place the EGFP tag as a synthetic exon into introns of target genes.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

   Insert

   EGFP
   

   Use

   Intron tagging donor

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19-Hph

   Plasmid

   #188741

   Purpose

   pUC19 plasmid (MCS intact) with Hygromycin selection marker

   Depositor

   Michael Bromley

   Article

   Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

   Insert

   Hygromycin
   

   Use

   Filamentous fungal expression

   Tags

   Expression

   Mutation

   Promoter

   gpdA

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5/FRT/TO_LAMP1-HaloTag11-T2A-Tomn20-HaloTag9

   Plasmid

   #175523

   Purpose

   Co-expression of HaloTag11 (lyzosome) and 9 (mitochondrial surface) in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   LAMP1-HaloTag11 and Tomm20-HaloTag9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HaloTag11 = HaloTag7-M175W and HaloTag9 = HaloTag…

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-T7-ABE8e-nSpCas9-NG-P2A-EGFP (KAC999)

   Plasmid

   #185914

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized ABE8e-nSpCas9-NG-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…

   Promoter

   CMV and T7

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETM6-vioABECD

   Plasmid

   #66535

   Purpose

   pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with consensus promoter

   Depositor

   Mattheos Koffas

   Article

   Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

   Inserts

   VioA
   VioB
   VioE
   VioC
   VioD
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Consensus T7

   Available since

   July 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. mEGFP-HRas

   Plasmid

   #18662

   Depositor

   Karel Svoboda

   Article

   Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91.

   Insert

   H-Ras (HRAS Human)
   

   Use

   Tags

   monomeric EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 2, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHL-avitag3

   Plasmid

   #99847

   Purpose

   mammalian expression with secretive signal and avi tag for biotinylation

   Depositor

   Edith Yvonne Jones

   Article

   Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

   Insert

   ephrin b2 (EPHB2 Human)
   

   Use

   Tags

   Avi

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCh-Rab7A

   Plasmid

   #61804

   Purpose

   Human Rab7A fluorescently tagged with mCh on the N-terminus for mammalian expression

   Depositor

   Gia Voeltz

   Article

   Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1

   Insert

   Rab7A (RAB7A Human)
   

   Use

   Tags

   mCh

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available since

   Feb. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pVDL9.3

    Plasmid

    #168299

    Purpose

    Secretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyA

    Depositor

    Víctor de Lorenzo

    Article

    Tzschaschel et al Nat Biotechnol. 1996 Jun;14(6):765-9. do

    Insert

    Segment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    LacI-Plac

    Available since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSUP-(2xCouRS-tRNAopt)

    Plasmid

    #197930

    Purpose

    Machinery plasmid expressing constitutive and inducible 7HCou synthetase and cognate amber suppressing tRNA. Incorporates (7-hydroxy-4-coumarin-yl) ethylglycine

    Depositor

    Kenneth Johnson

    Article

    Dangerfield et al J Biol Chem. 2020 Dec 11;295(50):17265-1

    Inserts

    CouRS
    tRNA opt
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    glnS' and lambda(ts) and proK

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCas9D10A_GFP

    Plasmid

    #44720

    Purpose

    Co-expression of human codon-optimized Cas9 (D10A) mutant nickase and GFP, plasmid optimized for expression in human pluripotent stem cells

    Depositor

    Kiran Musunuru

    Article

    Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

    Insert

    Cas9D10A-2A-GFP
    

    Use

    CRISPR

    Tags

    2A-GFP

    Expression

    Mammalian

    Mutation

    D10A mutation- nickase mutant

    Promoter

    CAG

    Available since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. CMV500 A-FOS

    Plasmid

    #33353

    Depositor

    Charles Vinson

    Article

    Ahn et al Mol Cell Biol. 1998 Feb . 18(2):967-77.

    Insert

    FOS (Fos Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Dominant Negative of c-Jun; heterodimerizes and i…

    Promoter

    CMV

    Available since

    Dec. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-C1-Nup35-mut

    Plasmid

    #87341

    Purpose

    To express human Nup35 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    NUP35 (NUP35 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    shRNA resistant changes

    Promoter

    CMV

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBITE-EGFRvIII-F265-OKT3

    Plasmid

    #190679

    Purpose

    Encodes an EGFRvIII-CD3 BITE protein (F265 clone)

    Depositor

    Scott McComb

    Article

    Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

    Insert

    Anti-EGFRvIII-scFv-F265-modularized-OKT3-HisTag
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. CIB81-pmGFP

    Plasmid

    #75366

    Purpose

    Expresses aa1-81 of Arabidopsis CIB1 fused to EGFP with CaaX membrane tag on end

    Depositor

    Chandra Tucker

    Article

    Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

    Insert

    CIB1 (CIB1 Mustard Weed)
    

    Use

    Tags

    prenylated EGFP

    Expression

    Mammalian

    Mutation

    residues 1-81 of CIB1

    Promoter

    CMV

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. FLAG-TRAF6-wt

    Plasmid

    #21624

    Depositor

    John Kyriakis

    Article

    Zhong et al Mol Cell Biol. 2004 Oct . 24(20):9165-75

    Insert

    TNF receptor-associated factor 6 (Traf6 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. OmpA-mNG-sfTq2ox

    Plasmid

    #124220

    Purpose

    Periplasmic OM bound mNeongreen superfolder mTurquoise2 ox tandem, ~40 % energy transfer. pNM092 - pTHV037-Ompa-SA1-177-(SA-1)-LEDPPAEL-mNG-sfTq2ox,

    Depositor

    Tanneke den Blaauwen

    Article

    Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

    Insert

    OmpA-mNG-sfTq2ox
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-T7-AcrIIA4-NLS(SV40) (KAC200)

    Plasmid

    #133801

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA4 with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized AcrIIA4
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CMV and T7

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-T7-AcrIIA5-NLS(SV40) (KAC203)

    Plasmid

    #133802

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA5 with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized AcrIIA5
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CMV and T7

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. pFR-mScarletI-antiFRET-P2A-G-Ca-FLITS

    Plasmid

    #191457

    Purpose

    Compare the intensity of the genetically encoded calcium sensor (GECI) G-Ca-FLITS to mScarlet-I fluorescent protein

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    G-Ca-FLITS
    

    Use

    Tags

    His-mScarletI-antiFRETlinker-P2A

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. pHR-CLIP-PD-L2

    Plasmid

    #223587

    Purpose

    Lentiviral expression of human PD-L2 with CLIP tag in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

    Insert

    PD-L2 (PDCD1LG2 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    CLIP

    Expression

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. pETM6-At4CL-PhCHS-CmCHI (C5 mutant, pFlavo-opt)

    Plasmid

    #73404

    Purpose

    Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

    Depositor

    Mattheos Koffas

    Article

    Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

    Inserts

    At4CL
    PhCHS
    CmCHI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLenti_CMV:Flag-GpNLuc

    Plasmid

    #208840

    Purpose

    Expresses FLAG epitope-tagged GpNLuc "Green" BRET reporter in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

    Insert

    GpNLuc LumiFluor
    

    Use

    Lentiviral

    Tags

    1xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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25. pcDNAintron-XKR8-n3xFLAG

    Plasmid

    #215693

    Purpose

    Produces the XKR8 lipid scramblase with an n-terminal 3xFLAG tag

    Depositor

    Melinda Brindley

    Article

    Acciani et al J Virol. 2021 Sep 27;95(20):e0116521. do

    Insert

    XKR8-n3xFLAG (XKR8 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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26. pRosetta_v2

    Plasmid

    #136477

    Purpose

    Lentiviral GFP Reporter assay for determining appropriate dose of puromycin, blasticidin, and hygromycin

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

    Insert

    HygroR-T2A-BlastR-P2A-PuroR-F2A-EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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27. pLentiRhoA2G

    Plasmid

    #40179

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    RhoA Biosensor
    

    Use

    Lentiviral

    Tags

    His-Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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28. pLenti-Synapsin-hChR2(H134R)-EYFP-WPRE

    Plasmid

    #20945

    Purpose

    3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by human Synapsin I promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    H134R mutation in ChR2

    Promoter

    hSyn

    Available since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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29. pet28a-His6-Keap1

    Plasmid

    #62454

    Purpose

    His6 tagged Keap1 for E.coli expression

    Depositor

    Yimon Aye

    Article

    Parvez et al J Am Chem Soc. 2014 Dec 31.

    Insert

    Keap1 (KEAP1 Human)
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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30. pME-MLS-KillerRed

    Plasmid

    #115517

    Purpose

    Middle entry plasmid containing membrane bound KillerRed fluorophore

    Depositor

    Marco Morsch

    Article

    Formella et al Redox Biol. 2018 Aug 23;19:226-234. doi:

    Insert

    MLS-KillerRed
    

    Use

    Gateway cloning

    Tags

    MLS

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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