We narrowed to 12,998 results for: cdna

1. GLUT1-eGFP/pcDNA-DEST47

   Plasmid

   #18729

   Depositor

   Wolf Frommer

   Article

   Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

   Insert

   pcDNA-DEST47 /hGLUT1-eGFP
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   deleted TAA

   Promoter

   Available since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. α-actinin-4-M522-Venus in pcDNA6

   Plasmid

   #190802

   Purpose

   Imaging of α-actinin-4 in live cells

   Depositor

   Vivian Tang

   Article

   Morris et al J Cell Biol. 2022 May 2;221(5). pii: 213

   Insert

   alpha-actiinin-4-M522-venus (ACTN4 Human, Synthetic)
   

   Use

   Tags

   His, myc, and venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. 1432 pcDNA3 flag MAGI1c

   Plasmid

   #10714

   Depositor

   William Sellers

   Article

   Vazquez et al J Biol Chem. 2001 Dec 28. 276(52):48627-

   Insert

   MAGI-1 (Magi1 Mouse)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 28, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Arf6 T27N- Venus in pcDNA3.1

   Plasmid

   #90464

   Purpose

   RhoA mediated cell migration

   Depositor

   Narasimhan Gautam

   Article

   Castillo-Badillo et al ACS Synth Biol. 2020 Mar 16. doi: 10.102

   Insert

   Arf6 T27N (ARF6 )
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCDNA3-AUP1-FLAG-DATDCUE

   Plasmid

   #185340

   Purpose

   Assessing domain requirement sof AUP1

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   AUP1 (AUP1 Human)
   

   Use

   Tags

   FLAG, 6xHis

   Expression

   Mammalian

   Mutation

   deletion of aa 90-337 corresponding to the acyl t…

   Promoter

   Available since

   May 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA5/FRT/TO-GAlphaz-RLuc8

   Plasmid

   #140978

   Purpose

   Encodes a G alpha subunit (GNAZ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GAlphaz-Rluc8 (GNAZ Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3-sACE2-WT(732)-IgG1

   Plasmid

   #154104

   Purpose

   Mammalian expression plasmid for dimeric soluble ACE2 (IgG1-Fc fusion)

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Use

   Tags

   Fc region of human IgG1

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease and neck domains …

   Promoter

   CMV

   Available since

   June 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-FLAG-mTET2 CD mutant

   Plasmid

   #89896

   Purpose

   Expresses mouse TET2 CD mutant in mammalian cells

   Depositor

   Yue Xiong

   Article

   Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

   Insert

   TET2 (Tet2 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   CD (cysteine-rich, dioxygenase domain) truncation…

   Promoter

   Available since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-mCherry-Rheb(C181S)

   Plasmid

   #192437

   Purpose

   Encodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant.

   Depositor

   Jin Zhang

   Article

   Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

   Insert

   mCherry-Rheb(C181S) (RHEB Human)
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Rheb Cys 181 mutated to Ser; abolishes C-terminal…

   Promoter

   CMV

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1/NDUFS2/myc-His

    Plasmid

    #127486

    Purpose

    Expresses myc-tagged NDUFS2 in mammalian cells

    Depositor

    Shashi Jain, Dieter Wolf

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFS2 (NDUFS2 Human)
    

    Use

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(+)/GUG-DAP5-3XFLAG

    Plasmid

    #127330

    Purpose

    Express 3xFLAG tagged DAP5 protein from GUG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    DAP5 (EIF4G2 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1-N-Myc_1-205 USP7

    Plasmid

    #131245

    Purpose

    Mammalian expression of the USP7 TRAF-like domain with an N-terminal Myc tag.

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Expresses a mutant form of our pcDNA3.1-N-Myc_1-5…

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-Venus-StagRFP-AktAR

    Plasmid

    #173020

    Purpose

    Yellow-red FRET-based Akt/PKB activity reporter for multiplexed imaging applications.

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

    Insert

    Venus-StagRFP-AktAR
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3 MDM2 S166D S186D

    Plasmid

    #16236

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

    Insert

    MDM2 (MDM2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Ser166 and Ser186 replaced by Asp

    Promoter

    Available since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.1/Myc-His(-)-MSulf-2

    Plasmid

    #13008

    Depositor

    Steven Rosen

    Article

    Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

    Insert

    mouse sulfatase 2 (Sulf2 Mouse)
    

    Use

    Tags

    6xHis and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA5/FRT miniAID-EGFP

    Plasmid

    #101713

    Purpose

    For amplification of a miniAID-EGFP for C-terminal tagging

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    miniAID-EGFP
    

    Use

    Tags

    miniAID-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1 Flag Runx1 FL (P#1790)

    Plasmid

    #14585

    Depositor

    Matija Peterlin

    Article

    Jiang et al Mol Cell Biol. 2005 Dec . 25(24):10675-8

    Insert

    Runx1 (Runx1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1-mCherry-CyrCR1.mutant

    Plasmid

    #128746

    Purpose

    Expresses mCherry in mammalian cells. ~200 nt of Cyrano with 5 point mutations in miR-7 site inserted in mCherry 3'UTR.

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    Cyrano conserved region (CR1) with mutant miR-7 site (OIP5-AS1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    5 point mutations disrupting the miR-7 site

    Promoter

    CMV

    Available since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1/hChR2(H134R)-EYFP

    Plasmid

    #20940

    Purpose

    Mammalian expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H134R mutation in ChR2

    Promoter

    CMV

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA4TO-mito-mCherry-24xGCN4_v1

    Plasmid

    #60913

    Purpose

    A fusion protein of the SunTag 24xGCN4 v1 peptide array to a mitochondrial targeting domain and mCherry

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    mito-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3.1-hLbCpf1(TYCV) (pY230)

    Plasmid

    #89355

    Purpose

    Expresses humanized LbCpf1 variant that recognizes TYCV PAMs

    Depositor

    Feng Zhang

    Article

    Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

    Insert

    Cpf1, RR variant
    

    Use

    CRISPR

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    G532R/K595R

    Promoter

    CMV

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3-N-Flag-NLRP3 91-710

    Plasmid

    #75140

    Purpose

    Expresses N-terminal Flag-tagged amino acids 91-710 of NLRP3 (the nucleotide-binding domain) in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    fragment encoding amino acids 91-710

    Promoter

    CMV

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA5/FRT/TO HIS DNAJA1

    Plasmid

    #19545

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    DnaJ (Hsp40) homolog, subfamily A, member 1 (DNAJA1 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1his p66shc S36A (corrected)

    Plasmid

    #32575

    Depositor

    Toren Finkel

    Article

    Nemoto et al Science. 2002 Mar 29. 295(5564):2450-2.

    Insert

    p66shc (SHC1 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    S36A

    Promoter

    CMV

    Available since

    Oct. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1-MS2CP-VPg(FCV)

    Plasmid

    #167314

    Purpose

    Template pDNA to prepare CaVT mRNA

    Depositor

    Hirohide Saito

    Article

    Nakanishi et al Cell Chem Biol. 2021 Jan 20. pii: S2451-

    Insert

    CaVT
    

    Use

    Synthetic Biology

    Tags

    DYK-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3-FLAG-Synaptojanin 1-170

    Plasmid

    #22292

    Depositor

    Pietro De Camilli

    Article

    Haffner et al FEBS Lett. 1997 Dec 15. 419(2-3):175-80.

    Insert

    Synaptojanin 1_170 (SYNJ1 Human)
    

    Use

    Tags

    Flag

    Expression

    Bacterial and Mammalian

    Mutation

    K334R compared to Genbank ID NM_003895

    Promoter

    Available since

    Oct. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.1(-)-EGFP-FP4-mito

    Plasmid

    #229692

    Purpose

    Transient expression of EGFP FP4-mito in mammalian cells

    Depositor

    Alpha Yap

    Article

    Scott et al Mol Biol Cell. 2006 Mar;17(3):1085-95. d

    Insert

    EGFP; Mitochondira FP4 motifs from Listeria ActA
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1(-)-EGFP-AP4-mito

    Plasmid

    #229693

    Purpose

    Transient expression of EGFP AP4-mito in mammalian cells

    Depositor

    Alpha Yap

    Article

    Scott et al Mol Biol Cell. 2006 Mar;17(3):1085-95. d

    Insert

    EGFP; Control construct with tandem AP4 motifs (mitochondria)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCDNA3-AUP1-FLAG DTM

    Plasmid

    #185337

    Purpose

    Assessing domain requirements of AUP1

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    AUP1 (AUP1 Human)
    

    Use

    Tags

    FLAG, 6xHis

    Expression

    Mammalian

    Mutation

    deletion of aa 24-45, corresponding to the hairpi…

    Promoter

    Available since

    Feb. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCDNA3-AUP1-FLAG-DCUE

    Plasmid

    #185339

    Purpose

    Assessing domain requirements of AUP1

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    AUP1 (AUP1 Human)
    

    Use

    Tags

    FLAG, 6xHis

    Expression

    Mammalian

    Mutation

    deletion of aa 293-337 corresponding to the CUE d…

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3-myc-FLNa S2152A

    Plasmid

    #8983

    Depositor

    John Blenis

    Article

    Woo et al Mol Cell Biol. 2004 Apr . 24(7):3025-35.

    Insert

    Filamin A S2152A (FLNA Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Ser2152Ala

    Promoter

    Available since

    Oct. 11, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3.1+CMV-Ins-GLuc

    Plasmid

    #89929

    Purpose

    Expresses Insulin-GLuc from the CMV promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.

    Depositor

    Melanie Cobb

    Article

    Kalwat et al ACS Sens. 2016 Oct 28;1(10):1208-1212. E

    Insert

    human insulin-Gaussia-Luciferase (INS Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    DNA sequence for Gaussia luciferase was humanized…

    Promoter

    CMV

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCDNA4-myc-His-mPGC-1a

    Plasmid

    #24925

    Depositor

    Toren Finkel

    Insert

    PGC-1a (Ppargc1a Mouse)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2010

    Availability

    Academic Institutions and Nonprofits only

34. pcDNA3-FLAG-MTOR-I2500F

    Plasmid

    #69014

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-I2500F (MTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Isoleucine 2500 to Phenylalanine

    Promoter

    Available since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3.1-Cerulean-Epac2-Venus

    Plasmid

    #138196

    Purpose

    FRET-based biosensor for monitoring Epac2 activation.

    Depositor

    Jin Zhang

    Article

    Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

    Insert

    Cerulean-Epac2-Venus (Rapgef4 Mouse, Synthetic)
    

    Use

    Tags

    Cerulean and Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3.1-N-Myc_1-560 USP7

    Plasmid

    #131244

    Purpose

    Mammalian expression of N-terminally Myc-tagged USP7, minus the UBL domains

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Expresses USP7 amino acids 1 - 560. This truncati…

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCDNA3.0_nucLAMA-G97 IRES EGFP

    Plasmid

    #136620

    Purpose

    Plasmid encoding nuclear localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    SNAP-Tag-LAMA-G97-NLS (IRES EGFP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA5-FRT/TO-eGFP-SHLD3

    Plasmid

    #114125

    Purpose

    Expresses N-terminally tagged eGFP-SHLD3 in mammalian cells

    Depositor

    Daniel Durocher

    Article

    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

    Insert

    SHLD3 (SHLD3 Human)
    

    Use

    Genomic integration, tetracycline inducible

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TetO2

    Available since

    Aug. 16, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. pcDNA5-FRT-TO-N-Flag-8His

    Plasmid

    #157677

    Purpose

    N-terminal tagging vector

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Flag-8His
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. pcDNA5/FRT/TO GFP HSPA1L

    Plasmid

    #19484

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    HSPA1L (HSPA1L Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge