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Showing: 2061 - 2080 of 26170 results

  3. pN1 Lck-GCaMP5G

    Plasmid
    #34924
    Purpose
    Expresses a fusion between the Lck domain and cytosolic GCaMP5G. This construct targets GCaMP5G to the plasma membrane
    Depositor
    Baljit Khakh
    Article
    Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.
    Insert
    Lck-GCaMP5G
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Plasmid contains N-terminal 26 amino acid membran…
    Promoter
    CMV
    Available since
    Feb. 21, 2012
    Availability
    Academic Institutions and Nonprofits only
    34924-plasmid-map-sequence-id-42429

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  5. pcDNA3.1-KozATG-dL5-2XG4S-actin

    Plasmid
    #73262
    Purpose
    Expresses dL5(E52D)-actin fusion within mammalian cells. (MBIC5, dL5**, FAP)
    Depositor
    Marcel Bruchez
    Article
    Yan et al Chemphyschem. 2014 Mar 17;15(4):687-95.
    Insert
    KozATG-dL5-2XG4S-actin (ACTB Human, Synthetic)
    Use
    Tags
    The FAP and actin are fused with 2 copies of a G4…
    Expression
    Mammalian
    Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L…
    Promoter
    CMV
    Available since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    73262-plasmid-map-sequence-id-137068

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  7. pML340_RAB11A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid
    #227907
    Purpose
    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
    Depositor
    Manuel Leonetti
    Article
    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.
    Insert
    RAB11A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    227907-plasmid-map-sequence-id-452503

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  9. elavl3:SomaGCaMP7f

    Plasmid
    #158760
    Purpose
    Cell body-targeted GCaMP7f, under HuC promoter: GCaMP7f followed by a linker, and the EE-RR coiled coil motif (GCaMP7f-27-EE-RR). As bright as conventional GCaMP7f.
    Depositor
    Edward Boyden
    Article
    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)
    Insert
    SomaGCaMP7
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    HuC promoter
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158760-plasmid-map-sequence-id-310667

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  10. pcDNA3.1(+)-NES-ZapCV2 (cpV143)

    Plasmid
    #112060
    Purpose
    Genetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the cytosol. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)
    Depositor
    Amy Palmer
    Article
    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi
    Insert
    NES-ZapCV2
    Use
    Tags
    Nuclear Export Signal (NES) derived from HIV-1
    Expression
    Mammalian
    Mutation
    ECFP gene is truncated 36 bp at 3' end. Venu…
    Promoter
    CMV
    Available since
    July 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    112060-plasmid-map-sequence-id-216934

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  18. pcDNA3.1-KozATG-dH6.2-2XG4S-actin

    Plasmid
    #73263
    Purpose
    Expresses dH6.2-actin fusion within mammalian cells. (dH6.2, FAP)
    Depositor
    Marcel Bruchez
    Article
    Yan et al Chemphyschem. 2014 Mar 17;15(4):687-95.
    Insert
    KozATG-dH6.2-2XG4S-actin (ACTB Human, Synthetic)
    Use
    Tags
    The FAP and actin are fused with 2 copies of a G4…
    Expression
    Mammalian
    Mutation
    The dH6.2 FAP has one cysteine in each domain cha…
    Promoter
    CMV
    Available since
    March 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    73263-plasmid-map-sequence-id-137070

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Showing: 2061 - 2080 of 26170 results