We narrowed to 13,743 results for: TIM

1. eMagAF-EGFP-Mito

   Plasmid

   #162243

   Purpose

   Blue-light dependent heterodimerization system

   Depositor

   Pietro De Camilli

   Article

   Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

   Insert

   eMagAF-EGFP-OMP25
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-Ec.coaD (pESC106)

   Plasmid

   #50388

   Purpose

   Heterologous expression of E. coli CoA biosynthetic enzyme, PPAT (phosphopantetheine adenylyltransferase). Contain 6xHis-tag for nickel affinity purification.

   Depositor

   Tadhg Begley, Erick Strauss

   Article

   Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

   Insert

   PPAT
   

   Use

   Tags

   6xHis-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFA6a-link-yoEGFP-CaURA3

   Plasmid

   #44872

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized EGFP with CaUra3 selection

   Depositor

   Kurt Thorn, Wendell Lim

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoEGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCIS.MTRIA-ATP

   Plasmid

   #184599

   Purpose

   For expression of a fluorescent sensor for ATP in mammalian cells

   Depositor

   Daisuke Ino, Hiroshi Hibino

   Article

   Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

   Insert

   MTRIA-ATP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5/FRT/TO_IgKchL_HA_HaloTag7_myc_PDGFRtmb

   Plasmid

   #175534

   Purpose

   Expression of HaloTag7 at the outer leaflet of the plasma membrane of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   IgKchL_HA-HaloTag7-myc_PDGFRtmb
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. OeNL/pcDNA3

   Plasmid

   #85202

   Purpose

   Orange color variant of bright luminescent protein

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   Orange enhanced Nano-lantern
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pAAV.Syn.MTRIA-OT

   Plasmid

   #184594

   Purpose

   For production of AAV for a fluorescent sensor for oxytocin

   Depositor

   Daisuke Ino, Hiroshi Hibino

   Article

   Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

   Insert

   MTRIA-OT
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Human synapsin promoter

   Available since

   June 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. ERoxBFP

   Plasmid

   #68126

   Purpose

   mammalian expression of ER localized oxBFP

   Depositor

   Erik Snapp

   Article

   Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

   Insert

   oxBFP
   

   Use

   Tags

   KDEL ER retention sequence and bovine prolactin s…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pME-Cas9

   Plasmid

   #64237

   Purpose

   expresses codon optimized Cas9 in zebrafish

   Depositor

   Wenbiao Chen

   Article

   Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

   Insert

   codon optimized Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-Ec.coaE (pESC124)

    Plasmid

    #50390

    Purpose

    Heterologous expression of E. coli CoA biosynthetic enzyme, DPCK (dephospho-coenzyme A kinase). Contain 6xHis-tag for nickel affinity purification.

    Depositor

    Tadhg Begley, Erick Strauss

    Article

    Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

    Insert

    DPCK
    

    Use

    Tags

    6XHIS tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pG-HIV-2LTR

    Plasmid

    #104590

    Purpose

    standard for qPCR

    Depositor

    Kristine Yoder

    Article

    Yoder et al J Virol Methods. 2008 Nov;153(2):253-6.

    Insert

    2LTR circle junction of HIV-1
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. Gq sensor

    Plasmid

    #137810

    Purpose

    A FRET sensor for Gq activation, encoded on a single plasmid

    Depositor

    Dorus Gadella

    Article

    Goedhart et al PLoS One. 2011;6(11):e27321. doi: 10.137

    Insert

    Gq sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFA6a-link-yomCherry-CaURA3

    Plasmid

    #44876

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mCherry with CaUra3 selection

    Depositor

    Kurt Thorn, Wendell Lim

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET21b Apaf1 1-591

    Plasmid

    #26642

    Depositor

    Guy Salvesen

    Article

    Pop et al Mol Cell. 2006 Apr 21. 22(2):269-75.

    Insert

    Apoptotic protease activating factor 1 (APAF1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Amino Acids 1-591

    Promoter

    Available since

    Nov. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

15. TroponinT-GCaMP5-Zeo

    Plasmid

    #46027

    Depositor

    John Gearhart

    Article

    Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

    Insert

    GCaMP5-2A-Zeo
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TNNT2

    Available since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJKR-H-cdaR

    Plasmid

    #62557

    Purpose

    Glucarate biosensor. High copy plasmid that expresses GFP proportional to glucarate concentration.

    Depositor

    George Church

    Article

    Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

    Inserts

    beta lactamase
    sfGFP
    CdaR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFA6a-link-yoPA-mCherry-Kan

    Plasmid

    #44950

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized photoactivatable mCherry with Kan selection

    Depositor

    Kurt Thorn, Wendell Lim

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoPA-mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-CyRFP1

    Plasmid

    #84356

    Purpose

    Expresses CyRFP1 fluorescent protein in an empty backbone, under CAG promoter

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    CyRFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFA6a-link-yoEGFP-SpHis5

    Plasmid

    #44836

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized EGFP with SpHis5 selection

    Depositor

    Kurt Thorn, Wendell Lim

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p177bp-LacOx162-Hyg

    Plasmid

    #210215

    Purpose

    Integrates 162xLacO repeats with a hygromycin drug selection marker into 177bp repeat region.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Insert

    162xLacO repeats derived from: PMID: 12864855.
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge