We narrowed to 24,628 results for: Nes

1. pGEX-4T-1 3xFlag-MAPKAPK2

   Plasmid

   #82720

   Purpose

   Expresses human MAPKAPK2

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   MAPKAPK2 (MAPKAPK2 Human)
   

   Use

   Tags

   3xFLAG and GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

2. pCF1148

   Plasmid

   #222330

   Purpose

   Fluorescent reporter plasmid for Veillonella

   Depositor

   J Christopher Fenno

   Article

   Goetting-Minesky et al Lett Appl Microbiol. 2024 Aug 5;77(8):ov

   Insert

   bs2 gene with Veillonella parvula SKV38 mdh promoter
   

   Use

   E. coli-veillonella shuttle plasmid

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Veillonella parvula SKV38 mdh promoter

   Available Since

   Oct. 31, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pEC3104 (pSpy1C-PgyrA(Sag)_ermBL-ermB'~ffluc)

   Plasmid

   #218513

   Purpose

   replicative E. coli-S. pyogenes shuttle plasmid for erythromycin-inducible expression of the Firefly luciferase

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Insert

   ffluc
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   deletion of the Plac_mrfp cassette

   Promoter

   PgyrA_ermBL-ermB'

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFUSE-rIgG-Fc2-MFSD6(L3)-Zeo

   Plasmid

   #239948

   Purpose

   Expression of MFSD6 loop 3 tagged with rabbit IgG Fc2

   Depositor

   Jan Carette

   Article

   Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

   Insert

   Fc2-MFSD6(L3)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available Since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. 2xP53_RE::NLuc

   Plasmid

   #124533

   Purpose

   p53 NLuc luciferase reporter

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

   Insert

   P53 NLuc reporter
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJSC111 - Bacterial expression plasmid for SpCas9-HF1 variant

   Plasmid

   #101209

   Purpose

   Bacterial expression plasmid for SpCas9-HF1 variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant N497A/R661A/Q695A/Q926A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   N497A, R661A, Q695A and Q926A

   Promoter

   T7

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBBK07 pCAS-Tyr-[gRNA: BsaI GFP dropout] (LEU2,AmpR)

   Plasmid

   #178991

   Purpose

   Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = LEU2

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLX302 OASL-V5 puro

   Plasmid

   #158643

   Purpose

   Lentiviral expression vector for constitutive expression of V5-tagged OASL.

   Depositor

   Kevin Janes

   Article

   Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

   Insert

   OASL (OASL Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-trGluc

   Plasmid

   #158959

   Purpose

   DNA donor plasmid containing repair template for Gaussia luciferase (Gluc) of bioluminescent repair reporter (BLRR).

   Depositor

   Charles P. Lai

   Article

   Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

   Insert

   trGluc
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX-4T-1 3xFlag-p38alpha

    Plasmid

    #47575

    Purpose

    Bacterial expression plasmid for GST-3xFlag-tagged p38alpha

    Depositor

    Kevin Janes

    Article

    Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

    Insert

    p38alpha (Mapk14 Mouse)
    

    Use

    Tags

    3xFlag and GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pSLIK 3xFLAG-CSE1L zeo

    Plasmid

    #192320

    Purpose

    Lentiviral expression vector for an inducible 3xFLAG-CSE1L

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    CSE1L (CSE1L Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX-4T-1 3xFlag-JNK1a1

    Plasmid

    #47574

    Purpose

    Bacterial expression plasmid for GST-3xFlag-tagged JNK1a1

    Depositor

    Kevin Janes

    Article

    Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

    Insert

    JNK1a1 (MAPK8 Human)
    

    Use

    Tags

    3xFlag and GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pLX302 OAS1-V5 puro

    Plasmid

    #158641

    Purpose

    Lentiviral expression vector for constitutive expression of V5-tagged OAS1.

    Depositor

    Kevin Janes

    Article

    Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

    Insert

    OAS1 (OAS1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMV-T7-hOsa2

    Plasmid

    #17987

    Depositor

    Naoko Tanese

    Article

    Inoue et al J Biol Chem. 2002 Nov 1. 277(44):41674-8

    Insert

    Osa2 (ARID1B Human)
    

    Use

    Tags

    T7 epitope

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC-GFP-HS

    Plasmid

    #133305

    Purpose

    "Burdensome" GFP expressing plasmid carrying hok/sok toxin-antitoxin for plasmid stabilisation

    Depositor

    Chris Barnes

    Article

    Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1

    Insert

    hok/sok toxin-antitoxin cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMax_mOrange_A215C

    Plasmid

    #177828

    Purpose

    Site-specific mutagenesis of mOrange for the purposes of creating a site-specific 8-oxo-G:C lesion for the purposes of measuring OGG1 activity via Host Cell Reactivation (HCR).

    Depositor

    Zachary Nagel, Leona Samson

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    mOrange_A215C
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A215C

    Promoter

    CMV

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. hCMV-IE1:NLuc

    Plasmid

    #118065

    Purpose

    Transcriptional unit for constitutive expression of NLuc in mammalian cells

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    CMV:NLuc:bGH
    

    Use

    Luciferase and Synthetic Biology; Assembly of gb2…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. p(A)n-PAUSE

    Plasmid

    #118068

    Purpose

    Transcription Blocker - Synthetic polyadenylation signal & RNA polymerase II transcriptional pause signal from the human α2 globin gene

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    Synthetic polyadenylation signal & RNA polymerase II transcriptional pause signal from the human α2 globin gene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEX-4T-1 3xHA-MEK1-DD

    Plasmid

    #47576

    Purpose

    Bacterial expression plasmid for GST-3xHA-tagged constitutively active MEK1

    Depositor

    Kevin Janes

    Article

    Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

    Insert

    MEK1-DD (Map2k1 Mouse)
    

    Use

    Tags

    3xHA and GST

    Expression

    Bacterial

    Mutation

    Ser218 to Asp, Ser221 to Asp

    Promoter

    Available Since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

20. hCMV-IE1:RedF

    Plasmid

    #118064

    Purpose

    Transcriptional unit for constitutive expression of RedF in mammalian cells

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    CMV:RedF:bGHF
    

    Use

    Luciferase and Synthetic Biology; Assembly of gb2…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEN_TT 3xFLAG-CSE1L

    Plasmid

    #192295

    Purpose

    Gateway entry vector for an inducible 3xFLAG-CSE1L

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    CSE1L (CSE1L Human)
    

    Use

    Gateway entry vector

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. AAEL005635-Cas9

    Plasmid

    #100608

    Purpose

    The promoter of AAEL005635 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

    Depositor

    Omar Akbari

    Article

    Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

    Inserts

    AAEL005635-hspcas9-GFP
    Opie2-dsRed
    

    Use

    CRISPR

    Tags

    GFP and dsRED

    Expression

    Insect

    Mutation

    Promoter

    AAEL005635 and Opie2

    Available Since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSLIK 3xFLAG-KPNA1 zeo

    Plasmid

    #192329

    Purpose

    Lentiviral expression vector for an inducible 3xFLAG-KPNA1

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    KPNA1 (KPNA1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHLuc (Antares-SEPLuc C1A4E+6)

    Plasmid

    #141089

    Purpose

    final optimized bioluminescent reporter for detecting extracellular pH changes in tumor acidosis

    Depositor

    Jeak Ling Ding

    Article

    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

    Insert

    Antares-T2A-puromycin-IRESv24-SEP-C1A4E+6
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMax_GFP_C289T

    Plasmid

    #177826

    Purpose

    Site-specific mutagenesis of GFP for the purposes of creating a site-specific hypoxanthine lesion for the purposes of measuring MPG activity via Host Cell Reactivation (HCR).

    Depositor

    Zachary Nagel, Leona Samson

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    GFP_C289T
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C289T

    Promoter

    CMV

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. hCMV-IE1:GrRenilla

    Plasmid

    #118067

    Purpose

    Transcriptional unit for constitutive expression of GrRenilla in mammalian cells

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    CMV:GreenRenilla:bGHF
    

    Use

    Luciferase and Synthetic Biology; Assembly of gb2…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Myc-Bnip3FL

    Plasmid

    #100796

    Purpose

    Mammalian expression of Bnip3 full length splice variant

    Depositor

    Joseph Gordon

    Article

    Diehl-Jones et al J Pediatr Gastroenterol Nutr. 2015 Nov;6

    Insert

    mus Bnip3 (Bnip3 Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. p5xNF-kb_RE

    Plasmid

    #118050

    Purpose

    GBPart - Operator/DNA Response element - 5 copies of the NF-kb DNA binding motif

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    5 copies of the NF-kb DNA binding motif
    

    Use

    Synthetic Biology; Domestication of dna parts for…

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGEX-4T-1 3xFlag-CREB

    Plasmid

    #82721

    Purpose

    Expresses human CREB

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    CREB (CREB1 Human)
    

    Use

    Tags

    3XFLAG and GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLX302-DUSP19-V5 puro

    Plasmid

    #87772

    Purpose

    Expresses V5 tagged human DUSP19

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    DUSP19 (DUSP19 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMax_BFP_A191G

    Plasmid

    #177823

    Purpose

    Site-specific mutagenesis of tagBFP for the purposes of creating a site-specific U:A mispair for the purposes of measuring UDG activity via Host Cell Reactivation (HCR).

    Depositor

    Zachary Nagel, Leona Samson

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    tagBFP_A191G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A191G

    Promoter

    CMV

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLX304-CAR-V5

    Plasmid

    #82723

    Purpose

    Expresses V5 tagged coxsackievirus and adenovirus receptor (CAR).

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    coxsackievirus and adenovirus receptor (CXADR Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

33. pcDNA3.2-DEST-MatryoshCaMP6s

    Plasmid

    #100025

    Purpose

    Mammalian expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    MatryoshCaMP6s
    

    Use

    Tags

    V5 Epitope Tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBBK94 pCAS-Tyr-[gRNA: 2xBsaI+NotI cut sites] (KanR)

    Plasmid

    #179078

    Purpose

    Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Kanamycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCDFDuet-MKK6-EE

    Plasmid

    #82718

    Purpose

    Expresses constitutively active human MKK6. Multiple cloning site 2 is empty.

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    MKK6 (MAP2K6 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Ser207 to Glu, Thr211 to Glu

    Promoter

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

36. tet_pLKO.1_puro_shLuc

    Plasmid

    #136587

    Purpose

    Expresses an inducible short hairpin targeting firefly luciferase sequence

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    shLuc
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. AAV-CREB

    Plasmid

    #68550

    Purpose

    AAV expression of CREB

    Depositor

    Eric Nestler

    Article

    Berger et al Biol Psychiatry. 2011 Feb 1;69(3):288-94

    Insert

    CREB
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCfB2904(XI-3 MarkerFree)

    Plasmid

    #73276

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-3 (Chr XI: 93378..94567)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. tet-pLKO shGATA6 puro

    Plasmid

    #72615

    Purpose

    Expresses an inducible short hairpin targeting human GATA6 sequence

    Depositor

    Kevin Janes

    Article

    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

    Insert

    short hairpin GATA binding protein 6
    

    Use

    Lentiviral

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    TRE Tight

    Available Since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. hCMV-IE1:ELuc

    Plasmid

    #118062

    Purpose

    Transcriptional unit for constitutive expression of ELuc in mammalian cells

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    CMV:ELuc:bGH
    

    Use

    Luciferase and Synthetic Biology; Assembly of gb2…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge