We narrowed to 6,502 results for: poly
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pHT-497
Plasmid#87756PurposeBacterial expression vector for producing 6x histidine tagged rat DYRK1A kinase domain (residues 1-497)DepositorInsertGene coding for rat DYRK1A kinase domain (residues 1-497)
UseTags6X histidine tagExpressionBacterialMutationPromoterT7 RNA polymerase promoterAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
FLAGHis10pFastBacHTA/Serca1
Plasmid#74401Purposeexpression of SERCA in insect cellsDepositorInsertSERCA1 (ATP2A1 rabbit)
UseTags10His and FLAGExpressionInsectMutationNcoI site destroyed (silent) T898PromoterpolyhedrinAvailable sinceApril 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
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PBRM1
Plasmid#39030PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertpolybromo 1 [isoform a] (PBRM1 Human)
UseTagsHis TevExpressionBacterialMutationcodon-optimizedPromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
4MYJ
Plasmid#60104PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertPF14_0346:MAC04G-A11:C228843
UseBaculovirus expressionTags6xHis-TEV and MHHHHHHSSGRENLYFQGExpressionMutationSee commentsPromoterPolyhedrinAvailable sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
p404GALL
Plasmid#17418DepositorInsertGALL promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationGALL promoter is between SacI and XbaI restrictio…PromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
PLXNB1
Plasmid#25352PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertPLXNB1:Y1511-L2135 (PLXNB1 Human)
UseBaculovirus expressionTagsHisExpressionMutationPromoterpolyhedrinAvailable sinceAug. 19, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
NSP12:4393-5324_Nbio_Chis_BV
Plasmid#234377PurposeBaculovirus expression for structure determination. May not contain entire coding region of gene.DepositorInsertNsp12_SARS2
UseBaculovirus expresssionTagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionMutationPromoterPolyhedrinAvailable sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
NSP13:5325-5925_BVnAvi
Plasmid#234375PurposeBaculovirus expression for structure determination. May not contain entire coding region of gene.DepositorInsertNsp13_SARS2
UseBaculovirus expresssionTagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionMutationPromoterPolyhedrinAvailable sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
SARS1_NSP13_BV_NBIO-TEV_CTHROM-his
Plasmid#234382PurposeBaculovirus expression for structure determination. May not contain entire coding region of gene.DepositorInsertNsp13_SARS
UseBaculovirus expresssionTagsLVPRGSGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionMutationPromoterPolyhedrinAvailable sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pD(P+T)_CMV
Plasmid#223860Purpose4G-cloning donor for mammalian promoter and terminator, no tag addedDepositorInsertCMV promoter and bGH polyA
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB His-TEV-BN-rNLRP1-FLAG
Plasmid#217323PurposePurification of Brown Norway rat NLRP1 fusion with C-terminal FLAG tag from insect cellsDepositorInsertNLRP1
UseTagsFLAG and His-TEVExpressionInsectMutationPromoterpolyhedrinAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB His-TEV-LEW-rNLRP1-FLAG
Plasmid#217320PurposePurification of Lewis rat NLRP1 fusion with C-terminal FLAG tag from insect cellsDepositorInsertNLRP1
UseTagsFLAG and His-TEVExpressionInsectMutationPromoterpolyhedrinAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-GFP_mmu-miR-16-5p_sponge
Plasmid#132624PurposeExpresses a TurboGFP transcript with microRNA sponge for mmu-miR-16-5p as 3'UTRDepositorInsertstop codon, mmu-miR-16-5p sponge sequence and polyA signal
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
UTP15:PBC028-C01:C245785
Plasmid#183973PurposeBaculovirus expression for structure determination. May not contain entire coding region of gene.DepositorInsertUTP15 (UTP15 Human)
UseBaculovirus expresssionTags6xHis-TEVExpressionMutationPromoterPolyhedrinAvailable sinceMay 10, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
LIPS:SDC101a-A08:C34411
Plasmid#183978PurposeBaculovirus expression for structure determination. May not contain entire coding region of gene.DepositorInsertLIPS
UseBaculovirus expresssionTagsC-terminal 8xHis tag:EFVEHHHHHHHH and N-termina…ExpressionMutationPromoterPolyhedrinAvailable sinceMay 10, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-ECOligA
Plasmid#165564PurposeConnects preferentially cohesive double-stranded DNA ends, active on blunt end DNA in the presence of Ficoll or polyethylene glycol. Requires Mg2+ and NAD+. Ligation when blunt end or RNA/ DNA ligation needs to be avoided.DepositorInsertE. coli DNA Ligase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 17, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTU1-D-spsCD
Plasmid#112788PurposeDownstream homology arm, SpsC is involved in spore coat polysaccharide synthesisDepositorArticleInsertspsCD
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A-spsCU
Plasmid#112787PurposeUpstream homology arm, SpsC is involved in spore coat polysaccharide synthesisDepositorArticleInsertspsCU
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOpen-BovDNTT
Plasmid#165580PurposeAdds dT at the end of PCR blunted DNA fragments/vectors that can then be used in TOPO cloning; Addition of homopolymer tails to the 3' ends of DNA; Labeling the 3' ends of DNA with modified nucleotides (e.g., ddNTP, DIG-dUTP); TUNEL assay (in situ localization of apoptosis); TdT dependent PCRDepositorInsertBovine DNTT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 28, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
47 pLIB_GST
Plasmid#160272PurposeN-term 3C cleavable GST tag for insect cell expressionDepositorTypeEmpty backboneUseTagsGSTExpressionInsectMutationPromoterpolyhydrinAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPGKPhiC31obpA
Plasmid#13795PurposeExpression of mouse codon-optimized PhiC31 recombinase with bovine growth hormone polyadenylation (bpA) sequence.DepositorInsertPhiC31o
UseTagsExpressionMammalianMutationPromoterPGKAvailable sinceJan. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
53 pLIB_MBP
Plasmid#160301PurposeN-term 3C cleavable MBP tag for insect cell expressionDepositorTypeEmpty backboneUseTags6xHis and MBPExpressionInsectMutationPromoterpolyhydrinAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1_FLAG-BPTF
Plasmid#102242PurposeExpression of human Flag-BPTF using baculovirus from pFastBac1DepositorInsertFlag-BPTF (BPTF Human)
UseTagsFlagExpressionInsectMutationPromoterPolyhedronAvailable sinceDec. 11, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
50 pLIB_STREP
Plasmid#160275PurposeN-term 3C cleavable 2xStrepII tag for insect cell expressionDepositorTypeEmpty backboneUseTags2xStrepIIExpressionInsectMutationPromoterpolyhydrinAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
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Stagia3
Plasmid#28177DepositorInsertStop TATA
UseChicken expressionTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
LEGO-GM55
Plasmid#217413PurposeLuciferase expression vector with the minimal GM-CSF promoterDepositorInsertLuciferase, GFP
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterHuman -55 to +28 minimal CSF2 promoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pRAV23
Plasmid#67715PurposeParental plasmid for transcription of RNAs with a GlmS/MS2 3'-tag for native purificationDepositorInsertTranscription cassette for 3'-tagged RNAs
UseTagsExpressionBacterialMutationPromoterT7 RNA polymeraseAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
56 pLIB_CBP
Plasmid#160305PurposeN-term 3C cleavable CBP tag for insect cell expressionDepositorTypeEmpty backboneUseTagsCBPExpressionInsectMutationPromoterpolyhydrinAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
52 pLIB_3C_C_STREP
Plasmid#160300PurposeC-term 3C cleavable 2xStrepII tag for insect cell expressionDepositorTypeEmpty backboneUseTags2xStrepIIExpressionInsectMutationPromoterpolyhydrinAvailable sinceNov. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pFHMSP-LIC-C
Plasmid#26100PurposeDonor vector to generate recombinant baculovirus by site-specific transposition into a baculovirus shuttle vector For use with Bac-to-Bac Expression System insect cells for secreted protein expressionDepositorTypeEmpty backboneUseBaculovirus expressionTags8x His tag and MKFLVNVALVFMVVYISYIYAAA (Honeybee …ExpressionInsectMutationPromoterpolyhedrinAvailable sinceAug. 20, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
251 pTol2Dest
Plasmid#73484Purposepromoterless Tol2 vector with attR1/attR2 cassette and late SV40 polyadenylation signalDepositorInsertattR1/attR2 cassette
UseTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
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57 pLIB_C_CBP
Plasmid#160306PurposeC-term uncleavable CBP tag for insect cell expressionDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterpolyhydrinAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
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p404GALS
Plasmid#17435DepositorInsertGALS promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationGALS promoter is between SacI and XbaI restrictio…PromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
ULK3 (ULK3A-c002)
Plasmid#110272PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertULK3 (ULK3 Human)
UseTagsHis6-ZBasic-TEVExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
44 pLIB_12HIS
Plasmid#160186PurposeN-term 3C cleavable 12xHis tag for insect cell expressionDepositorTypeEmpty backboneUseTags12xHisExpressionInsectMutationPromoterpolyhydrinAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
59 pLIB_HIS_HA
Plasmid#160308PurposeN-term 3C cleavable His-HA tag for insect cell expressionDepositorTypeEmpty backboneUseTags12xHis and 3xHAExpressionInsectMutationPromoterpolyhydrinAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
NUDT17 (NUDT17A-c014)
Plasmid#98224PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertNUDT17A (NUDT17 Human)
UseBaculovirusTagsHis6-ZBasic-TEVExpressionMutationpfam00293:NUDIX 93-235Promoterpolyhedrin promoterAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
45 pLIB_C_12HIS
Plasmid#160271PurposeC-term uncleavable 12xHis tag for insect cell expressionDepositorTypeEmpty backboneUseTags12xHisExpressionInsectMutationPromoterpolyhydrinAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
46 pLIB_3C_C_HIS
Plasmid#160188PurposeC-term 3C cleavable His tag for insect cell expressionDepositorTypeEmpty backboneUseTags12xHisExpressionInsectMutationPromoterpolyhydrinAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHT-PRD
Plasmid#87755PurposeBacterial expression vector for producing 6x histidine tagged dynamin 1a proline rich domain (residues 746-864)DepositorInsertSemi-synthetic gene coding for dynamin 1a proline rich domain (residues 746-864)
UseTags6X histidine tagExpressionBacterialMutationPromoterT7 RNA polymerase promoterAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRAV24
Plasmid#67716PurposeParental plasmid for transcription of RNAs with a GlmS/M-domain 3'-tag for native purificationDepositorInsertTranscription cassette for 3'-tagged RNAs
UseTagsExpressionBacterialMutationPromoterT7 RNA polymeraseAvailable sinceSept. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
NSP13:5325-5925_BV
Plasmid#234374PurposeBaculovirus expression for structure determination. May not contain entire coding region of gene.DepositorInsertNsp13_SARS2
UseBaculovirus expresssionTagsMHHHHHHSSGRENLYFQGExpressionMutationPromoterPolyhedrinAvailable sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only
