We narrowed to 15,037 results for: pET

1. pET-pro-siRNA-V2-TP53

   Plasmid

   #111089

   Purpose

   pET-pro-siRNA-V2 based plasmid for production of recombinant siRNAs (pro-siRNAs) against human TP53 gene.

   Depositor

   Linfeng HUANG

   Article

   Kaur et al Biotechnol Bioeng. 2018 Jun 5. doi: 10.1

   Insert

   TP53 (TP53 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-deSpCas9-VP64-6xHis

   Plasmid

   #92117

   Purpose

   Expression of dead/inactive increased fidelity eSpCas9 (1.1)-VP64-6xHis in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive eSpCas9-NLS-3xFLAG-VP64
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, NLS, and VP64

   Expression

   Bacterial

   Mutation

   D10A, H840A, K848A, K1003A, R1060A

   Promoter

   T7

   Available Since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-AMPKsub(TA)-YPet-NES

   Plasmid

   #84636

   Purpose

   Encodes negative-control C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   AMPKsub(TA)-YPet-NES
   

   Use

   Tags

   6xHis, Nuclear export signal (NES), T7 tag (gene …

   Expression

   Mammalian

   Mutation

   Target Thr residue in substrate domain mutated to…

   Promoter

   CMV

   Available Since

   April 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. HOXA4 (murine) HA-tag pET

   Plasmid

   #8489

   Depositor

   Corey Largman

   Article

   Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

   Insert

   HOXA4 (Hoxa4 Mouse)
   

   Use

   Tags

   HA and T7

   Expression

   Bacterial

   Mutation

   Thr-2 changed to Ala-2 to form better ribosomal b…

   Promoter

   Available Since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pET24a-VHH-tev-std

   Plasmid

   #109423

   Purpose

   Bacterial expression of a functionalized anti-GFP (VHH) nanobody with a TEV site. VHH-tev-std is flanked by an N-terminal T7 epitope, and a C-terminal cassette composed of HA, BAP and a His6 epitope

   Depositor

   Martin Spiess

   Article

   Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

   Insert

   anti-GFP nanobody fused to a T7, TEV cleavage site, HA, BAP and His6 epitope
   

   Use

   Tags

   BAP, HA, His6, and T7

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28-sfGFP-DogTag Loop B

   Plasmid

   #171780

   Purpose

   Expresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Asp102 and Asp103

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   sfGFP-DogTag Loop B
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   DogTag and linkers inserted between residues Asp1…

   Promoter

   T7

   Available Since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET29b-dspB(E184Q)-6xHis

   Plasmid

   #175802

   Purpose

   Plasmid for overexpression of recombinant His-tagged protein DspB(E184Q), used as a probe for binding to biofilm polysaccharide PNAG.

   Depositor

   Mark Nitz

   Article

   Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

   Insert

   Dispersin B
   

   Use

   Tags

   Hexahistidine tag

   Expression

   Bacterial

   Mutation

   E184Q

   Promoter

   T7

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-51b(+) ChemoG 3.2

   Plasmid

   #193803

   Purpose

   Protein production of ChemoG3.2 in bacteria

   Depositor

   Julien Hiblot

   Article

   Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

   Insert

   ChemoG 3.2
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   EGFP:A206K, T225R; HT7: L271E

   Promoter

   T7

   Available Since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET21a(+)-Histag-Citrine (525)

   Plasmid

   #70718

   Purpose

   Expression vector for Citrine purification

   Depositor

   Richard Murray

   Article

   Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

   Insert

   Citrine
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a SV40 ST full length

    Plasmid

    #132636

    Purpose

    expresses SV40 ST full length in E. coli

    Depositor

    Wenqing Xu

    Article

    Cho et al PLoS Biol. 2007 Aug;5(8):e202. doi: 10.1

    Insert

    SV40 small t antigen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-PKCsub-YPet-NES

    Plasmid

    #138207

    Purpose

    Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES

    Depositor

    Jin Zhang

    Article

    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

    Insert

    PKCsub-YPet-NES
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-17b_Unc104-KIF5B-HALO

    Plasmid

    #169996

    Purpose

    Ultra processive kinesin KIF1A fused with KIF5B stalk for dimerization and HALO-tag for labeling

    Depositor

    Ron Vale

    Article

    Niekamp et al Nat Methods. 2020 Apr;17(4):437-441. doi

    Insert

    Kinesin-like protein Unc-104 fused with KIF5B
    

    Use

    Tags

    HALO-tag, 6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET23-CBD-(-PP)-EGFP-H246,249D

    Plasmid

    #12598

    Depositor

    Klaus Hahn

    Article

    Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

    Type

    Empty backbone

    Use

    Tags

    6xHis and EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pET-SUMO mCER-Prx2

    Plasmid

    #158226

    Purpose

    Genetically encoded HomoFRET-based probe to monitor peroxiredoxin2 (Prx2) oligomerization (Recombinant bacterial expression)

    Depositor

    Tobias Dick

    Article

    Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

    Insert

    mCER-Prx2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET21a(+)_C.jejuni m23 peptidase

    Plasmid

    #83297

    Purpose

    Expresses m23 peptidase from Campylobacter jejuni

    Depositor

    SeWon Suh

    Insert

    ERS445056_01343
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    deleted amino acid 1-20

    Promoter

    Available Since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-ClfB_N2N3-HIS-ybbr

    Plasmid

    #101717

    Purpose

    ClfB N2 and N3 domains with C-terminal ybbr tag for covalent immobilization

    Depositor

    Hermann Gaub

    Article

    Milles et al Science. 2018 Mar 30;359(6383):1527-1533

    Insert

    ClfB_N2N3
    

    Use

    Tags

    H3V 3C, HIS, and ybbr

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28-H12TEV-hEIF5B-1~1220(FL)

    Plasmid

    #208636

    Purpose

    for E. coli expression of human eIF5B (ENST00000289371)

    Depositor

    Quanfu Ma

    Insert

    human eIF5B (ENST00000289371) (EIF5B Human)
    

    Use

    Tags

    6xhis

    Expression

    Bacterial

    Mutation

    Contain SNP rs1376443, protein sequence not chang…

    Promoter

    T7

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21b GFP-Rab10 Q68LHis

    Plasmid

    #186015

    Purpose

    GFP-Rab10 Q68LHis

    Depositor

    Suzanne Pfeffer

    Article

    Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

    Insert

    GFP-Rab10 Q68LHis (RAB10 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. PET-B-HypaSpCas9-NLS-6xHis

    Plasmid

    #207386

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HypaSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N692A, M694A, Q695A, H698A, amino acids 1005-1013…

    Promoter

    T7

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-6xHis-GFP(+36)-DL4

    Plasmid

    #199168

    Purpose

    used to express a supercharged GFP variant that forms inclusion bodies in E. coli

    Depositor

    Allie Obermeyer

    Article

    Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

    Insert

    GFP(+36)-DL4
    

    Use

    Tags

    MGHHHHHHGGA

    Expression

    Mutation

    E16R, T19R, V21K, D29K, E42K, E44K, N49E, D86K, Q…

    Promoter

    T7

    Available Since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. pET23b-Casp9 C287A-His

    Plasmid

    #11830

    Depositor

    Guy Salvesen

    Article

    Stennicke et al J Biol Chem. 1999 Mar 26. 274(13):8359-6

    Insert

    Caspase 9 catalytic mutant (CASP9 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    C287A

    Promoter

    Available Since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

22. pETNKI-his-3C-LIC-kan

    Plasmid

    #108703

    Purpose

    Expression of an His-tagged target protein with 3C protease cleavage site.

    Depositor

    Titia Sixma

    Article

    Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

    Type

    Empty backbone

    Use

    Tags

    His-3C protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. pETDuet1-WT-LanCL2-C-Ter-His

    Plasmid

    #154186

    Purpose

    To express LanCL2 in bacterial cells

    Depositor

    Wilfred van der Donk

    Article

    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

    Insert

    LanCL2
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. 3019_pETcon-SARS-CoV-2-RBD_E484K

    Plasmid

    #184404

    Purpose

    yeast surface display of the SARS-CoV-2 Eta variant RBD

    Depositor

    Jesse Bloom

    Article

    Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

    Insert

    SARS-CoV-2 Eta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
    

    Use

    Tags

    HA and c-Myc

    Expression

    Yeast

    Mutation

    E484K

    Promoter

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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25. pET28a-HsSENP3(307-574)

    Plasmid

    #71481

    Purpose

    Encodes the catalytic domain of human SENP3. Suitable for in vitro transcription/translation (T7).

    Depositor

    Jorge Eduardo Azevedo

    Article

    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

    Insert

    SUMO1/sentrin specific peptidase 3 (SENP3 Human)
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Deleted amino acids 1-306 (catalytic domain only)

    Promoter

    T7

    Available Since

    Dec. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

26. pET-DEST42-ARL8B-V5-His6

    Plasmid

    #67425

    Purpose

    Expression of hArl8b with C-term V5 and His6 tag in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL8B (ARL8B Human)
    

    Use

    Tags

    His and V5

    Expression

    Bacterial

    Mutation

    Promoter

    T7/lacO

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

27. pET-DEST42-ARF1-V5-His6

    Plasmid

    #67411

    Purpose

    Expression of hArf1 with C-term V5 and His6 tag in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARF1 (ARF1 Human)
    

    Use

    Tags

    His and V5

    Expression

    Bacterial

    Mutation

    Promoter

    T7/lacO

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

28. pET22-6His-SUMO-Nsp10

    Plasmid

    #154960

    Purpose

    Expresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli

    Depositor

    Justin Kollman

    Insert

    6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    6His-Thrombin-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available Since

    July 21, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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29. pET28a-hDcp2 E147Q, E148Q

    Plasmid

    #72215

    Purpose

    bacterial expression of His-tagged human Dcp2 Q147/8 mutant (disrupts the decapping function of Dcp2)

    Depositor

    Megerditch Kiledjian

    Article

    Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

    Insert

    Dcp2 (DCP2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    E147Q, E148Q

    Promoter

    T7

    Available Since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

30. pET21-GFP-AURKA-mCherry

    Plasmid

    #99876

    Purpose

    Expression of GFP-AURKA-mCherry for protein purification in bacteria

    Depositor

    Marc Tramier

    Article

    Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Use

    Tags

    GFP and mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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