We narrowed to 5,067 results for: codon optimized

1. pDONR221_SLC22A5

   Plasmid

   #132048

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC22A5 (SLC22A5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 4, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pDONR221_SLC7A8

   Plasmid

   #132233

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC7A8 (SLC7A8 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 8, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pET28b-DiCas7-11-His

   Plasmid

   #234482

   Purpose

   Plasmid for bacterial expression and purification of DiCas7-11 protein (human codon-optimized)

   Depositor

   Miguel Angel Moreno-Mateos

   Article

   Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

   Insert

   DiCas7-11
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDONR221_SLC22A15

   Plasmid

   #132011

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC22A15 (SLC22A15 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 25, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pDONR221_SLC1A2

   Plasmid

   #131901

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC1A2 (SLC1A2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pDONR221_SLC39A1

   Plasmid

   #132058

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC39A1 (SLC39A1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 8, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pET-32a(+)-TGFB3

   Plasmid

   #120288

   Purpose

   Produce human recombinant TGFB3 in DE3 cells

   Depositor

   Paul Burridge

   Article

   Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

   Insert

   Transforming Growth Factor-β3 (TGFB3 Human, Synthetic)
   

   Use

   Tags

   6x His and S-Tag

   Expression

   Bacterial

   Mutation

   Codon optimized for E. Coli production

   Promoter

   T7 promoter

   Available since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDONR221_SLC18A2

   Plasmid

   #131997

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC18A2 (SLC18A2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 22, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. ABE8e-Cas9NG

   Plasmid

   #203328

   Purpose

   Plasmid for bacterial purification of codon optimized ABE8e-Cas9NG

   Depositor

   Vijay Sankaran

   Article

   Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

   Insert

   ABE8e-Cas9NG
   

   Use

   CRISPR

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. U6-BbsI-DR_CMV-minidCas13X.1-REPAIRv2-BGHpA_CMV-EGFP-BGHpA

    Plasmid

    #171383

    Purpose

    Expression vector for encoding a human codon-optimized minidCas13X.1-REPAIRv2 driven by CMV promoter,EGFP driven by CMV promoter and U6-driven crRNAs cloning site.

    Depositor

    Hui Yang

    Article

    Xu et al Nat Methods. 2021 May;18(5):499-506. doi

    Insert

    humanized minidCas13X.1-REPAIRv2
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, U6

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CMV-dCas13X.1-REPAIRv2-SV40pA_CMV-mCherry-BGHpA_U6-BbsI-DR

    Plasmid

    #171382

    Purpose

    Expression vector for encoding a human codon-optimized dCas13X.1-REPAIRv2 driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

    Depositor

    Hui Yang

    Article

    Xu et al Nat Methods. 2021 May;18(5):499-506. doi

    Insert

    humanized dCas13X.1-REPAIRv2
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, U6

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CMV-Cas13X.1-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA

    Plasmid

    #171379

    Purpose

    Expression vector for encoding a human codon-optimized Cas13X.1 driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

    Depositor

    Hui Yang

    Article

    Xu et al Nat Methods. 2021 May;18(5):499-506. doi

    Insert

    humanized Cas13X.1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, U6

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pdCas9-DNMT3A-PuroR

    Plasmid

    #71667

    Purpose

    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A S. pyogenes, Human, Synthetic)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-PuroR

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9

    Promoter

    CBh

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDONR221_SLC25A3

    Plasmid

    #132040

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC25A3 (SLC25A3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pET28b-SpRY-His

    Plasmid

    #179320

    Purpose

    Plasmid for bacterial expression and purification of SpRY-Cas9

    Depositor

    Miguel Angel Moreno-Mateos, Julián Cerón

    Article

    Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

    Insert

    Human codon-optimized Cas9 variant SpRY
    

    Use

    CRISPR

    Tags

    6xHis and SV40-NLS

    Expression

    Bacterial

    Mutation

    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/ N13…

    Promoter

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pdCas9-DNMT3A-EGFP (ANV)

    Plasmid

    #71685

    Purpose

    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-EGFP; cloning backbone for sgRNA

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A S. pyogenes, Human, Synthetic)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; E756A inactiva…

    Promoter

    CBh

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDONR221_SLC16A1

    Plasmid

    #131934

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC16A1 (SLC16A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pDONR221_SLC1A4

    Plasmid

    #131962

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC1A4 (SLC1A4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pDONR221-NPC1

    Plasmid

    #132295

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    NPC1 (NPC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 14, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. FLAG-FBXL17

    Plasmid

    #236188

    Purpose

    Expression of N-terminal tagged FLAG-FBXL17 (H. sapiens, codon-optimized) in human cell lines.

    Depositor

    Michele Pagano

    Article

    Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

    Insert

    FLAG-FBXL17 (FBXL17 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDONR221_SLC38A3

    Plasmid

    #132142

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC38A3 (SLC38A3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 25, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. pDONR221_SLC7A10

    Plasmid

    #132232

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC7A10 (SLC7A10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pDONR221_SLC25A12

    Plasmid

    #131938

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC25A12 (SLC25A12 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 17, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pDONR221_SLC1A5

    Plasmid

    #131974

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC1A5 (SLC1A5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 18, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pdCas9-DNMT3A-PuroR (ANV)

    Plasmid

    #71684

    Purpose

    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR; cloning backbone for sgRNA

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A S. pyogenes, Human, Synthetic)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-PuroR

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; E756A inactiva…

    Promoter

    CBh

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pX330-U6-Chimeric_BB-CBh-hSpCas9-hGem(1/110)

    Plasmid

    #71707

    Purpose

    A human codon-optimized SpCas9 fused to first 110 amino acids of human GEMININ and chimeric guide RNA expression plasmid

    Depositor

    Giulio Draetta

    Article

    Gutschner et al Cell Rep. 2016 Feb 3. pii: S2211-1247(16

    Insert

    humanized S. pyogenes Cas9 fused to human Geminin (GMNN Human)
    

    Use

    CRISPR

    Tags

    3xFLAG and hGEM(1/110)

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDONR221_SLC25A5

    Plasmid

    #132007

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC25A5 (SLC25A5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 24, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pDONR221_SLCO2A1

    Plasmid

    #131949

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLCO2A1 (SLCO2A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 18, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pDONR221_SLC2A1

    Plasmid

    #132103

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC2A1 (SLC2A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 3, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pPB-cT3G-cERP2-GATA4

    Plasmid

    #192896

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of GATA4

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    GATA4 (GATA4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Codon-optimized

    Promoter

    TET3G-miniCMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pHLS-EF1a-FRB-SpCas9-A

    Plasmid

    #138477

    Purpose

    Expresses FRB fused SpCas9 for NanoMEDIC packaging.

    Depositor

    Akitsu Hotta

    Article

    Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

    Insert

    SpCas9, human codon optimized
    

    Use

    CRISPR

    Tags

    3x HA tag and FRB

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLenti-opCas12a-6xNLS

    Plasmid

    #209022

    Purpose

    Lentiviral vector expressing human codon-optimized OpCas12a with 6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    opCas12a-6xNLS-Flag-Myc-NeoR
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG and MYC

    Expression

    Mammalian

    Mutation

    E174R/S542R

    Promoter

    EF-1a

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. CMV-Cas13Y.1-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA

    Plasmid

    #171381

    Purpose

    Expression vector for encoding a human codon-optimized Cas13Y.1 driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

    Depositor

    Hui Yang

    Article

    Xu et al Nat Methods. 2021 May;18(5):499-506. doi

    Insert

    humanized Cas13Y.1
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, U6

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCBh_3x Flag-NLS-enOsCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid

    #197026

    Purpose

    Vector encoding a human codon-optimized enOsCas12f1 driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Xiangfeng Kong

    Article

    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

    Insert

    humanized enOsCas12f1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D52R / T132R

    Promoter

    CBh, CMV, hU6

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pRM053 His-TEV-Cas9 triNLS 0C

    Plasmid

    #196244

    Purpose

    Expression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)

    Depositor

    Ross Wilson

    Article

    Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    HA, His6 (N terminal on backbone), NLS, Nucleopla…

    Expression

    Bacterial

    Mutation

    Cys at positions 80 and 574 have been mutated to …

    Promoter

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pDONR221_SLC35F1

    Plasmid

    #132133

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC35F1 (SLC35F1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. pDONR221_SLC25A24

    Plasmid

    #131980

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC25A24 (SLC25A24 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. pET28b-SpG-His

    Plasmid

    #179318

    Purpose

    Plasmid for bacterial expression and purification of SpG-Cas9

    Depositor

    Miguel Angel Moreno-Mateos, Julián Cerón

    Article

    Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

    Insert

    Human codon-optimized Cas9 variant SpG
    

    Use

    CRISPR

    Tags

    6xHis and SV40-NLS

    Expression

    Bacterial

    Mutation

    SpG=D1135L/S1136W/G1218K/E1219Q/ R1335Q/T1337R

    Promoter

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDONR221_SLC25A4

    Plasmid

    #131982

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC25A4 (SLC25A4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 22, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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40. pRM049 His-TEV-Cas9 triNLS 2C

    Plasmid

    #196245

    Purpose

    Expression of NLS-rich Cas9 (2 Cys residues, mammalian codon optimized)

    Depositor

    Ross Wilson

    Article

    Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    HA, His6 (N terminal on backbone), NLS, Nucleopla…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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