We narrowed to 9,280 results for: Pol
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Plasmid#212716PurposeBacterial expression of His6-Mms2, His6-tag cleavable with TEV-proteaseDepositorInsertMms2
Tags6xHis-TEVExpressionBacterialAvailable SinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAL-TEV2-Ubc7-His6
Plasmid#212713PurposeBacterial expression of MBP-Ubc7-His6, MBP-tag cleavable with TEV-proteaseDepositorInsertUbc7
Tags6xHis and MBP-TEV2ExpressionBacterialAvailable SinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pA ins:TDimer mVenus
Plasmid#210520PurposeExpresses a tandem dimer of mVenus in zebrafish pancreatic beta cells using a zebrafish insulin promoterDepositorInsertTandem dimer mVenus
UseZebrafish expression, tol2 kit gateway-compatiblePromoterZebrafish insulin promoterAvailable SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
WNV NS2B-NS3 protease (based on PDB 2GGC, H106A - catalytically inactive); aliases: West Nile Virus NS2B-NS3 fusion protein
Plasmid#204797PurposeBacterial expression of a fusion of West Nile NS2B-NS3 proteinsDepositorInsertWest Nile NS2B-NS3 fusion
ExpressionBacterialAvailable SinceDec. 11, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMx/ATP11C-c-HA
Plasmid#209239PurposeMammalian expression of ATP11C isoform cDepositorAvailable SinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMx/ATP11C-b-HA
Plasmid#209238PurposeMammalian expression of ATP11C isoform bDepositorAvailable SinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
p35S::SP-mCherry-GFP-HDEL
Plasmid#159097PurposePositive control for FRET in plant ER / nuclear envelope.DepositorInsertSP-mCherry-GFP-HDEL
TagsmCherry, GFPExpressionPlantPromoter35SAvailable SinceOct. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-IEo
Plasmid#201992PurposeAAV vector mediating inducible expression of codon optimized immediate early gene IE180 of pseudorabies virusDepositorInsertInducible expression of codon optimized immediate early gene IE180 of pseudorabies virus
UseAAVPromoterTight TREAvailable SinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
TBPL1 KO 1
Plasmid#200210PurposegRNA for TBPL1 KODepositorAvailable SinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
TBPL1 KO 2
Plasmid#200211PurposegRNA for TBPL1 KODepositorAvailable SinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-IC2N-dCer3
Plasmid#191325PurposeChronically inhibit dynein by expressing the dominant negative acting N-terminal domain of the dynein intermediate chain (IC2N)DepositorAvailable SinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOP004
Plasmid#185985PurposeEncodes a 0.8 kb bacterial transcription unit with Broccoli-tRNA fluorogenic aptamer shortly upstream of the intrinsic terminatorDepositorInsertBacterial transcription unit encoding Broccoli-tRNA fluorogenic aptamer
ExpressionBacterialAvailable SinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOP005
Plasmid#186030PurposeEncodes a 0.8 kb bacterial transcription unit with Broccoli-tRNA fluorogenic aptamer shortly downstream of the promoterDepositorInsertBacterial transcription unit encoding Broccoli-tRNA fluorogenic aptamer
ExpressionBacterialAvailable SinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB221
Plasmid#185071PurposeSST::ACT2 "SA236"DepositorInsertACT2
ExpressionYeastMutationACT2-SA236Available SinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB215
Plasmid#185068PurposeSST::WAK2 EcoRI/ScaI fragment (secretion confirmed)DepositorInsertWAK2
ExpressionYeastAvailable SinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
NCH2exp
Plasmid#131019PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertNCH2
TagseGFP-FLAG-HAExpressionPlantPromoterP35SAvailable SinceMay 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
MNEAP1ec
Plasmid#131020PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertMNEAP1
UseGateway entry vectorTagseGFP-FLAG-HAAvailable SinceMay 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
MNEAP1exp
Plasmid#131021PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertMNEAP1
TagseGFP-FLAG-HAExpressionPlantPromoterP35SAvailable SinceMay 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
MKAKU41ec
Plasmid#131014PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertMKAKU41
UseGateway entry vectorTagsmCherry-FLAG-HAAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
MKAKU41exp
Plasmid#131015PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertMKAKU41
TagsmCherry-FLAG-HAExpressionPlantPromoterP35SAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
NCH1ec
Plasmid#131016PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertNCH1
UseGateway entry vectorTagseGFP-FLAG-HAAvailable SinceMay 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
NCH1exp
Plasmid#131017PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertNCH1
TagseGFP-FLAG-HAExpressionPlantPromoterP35SAvailable SinceMay 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
NCH2ec
Plasmid#131018PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertNCH2
UseGateway entry vectorTagseGFP-FLAG-HAAvailable SinceMay 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
gMUM2-Citrine
Plasmid#181965PurposeMUM2 protein fused to Citrine, under control of native promoterDepositorAvailable SinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
MUM2SP-Citrine
Plasmid#181963PurposeMUM2 signal peptide fused to Citrine, under control of MUM4_1.5kb promoterDepositorInsertMUM2 signal peptide, fused to the Citrine YFP in pAD
TagsCitrine YFPExpressionPlantPromoterMUM4pro 1.5kb fragmentAvailable SinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
CDS_Cas9-NLS
Plasmid#136121PurposePuchta Arabidopsis codon-optimised Cas9 for CRISPRDepositorInsertCDS_AtcoCas9-NLS
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
CTAG_eGFP-GHtag-HDEL
Plasmid#136096PurposeFP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptide. Contains Gly-His tagDepositorInsertCTAG_eGFP(noATG,noStop)-3xGly5xHys-HDEL
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
tcTRP24sub8
Plasmid#175796Purposede novo thick circular tandem repeat protein trimer formed from 8-repeat monomersDepositorInserttcTRP24sub8
Tags6xHisExpressionBacterialAvailable SinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
tcTRP24sub8_SS
Plasmid#175798Purposede novo thick circular tandem repeat protein trimer formed from 8-repeat monomers containing disulfide 'staples'DepositorInserttcTRP24sub8_SS
Tags6xHisExpressionBacterialAvailable SinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
tcTRP24sub8_SH2sub6
Plasmid#175799Purposede novo thick circular tandem repeat protein trimer formed from 8-repeat monomers containing 2 copies of human Nck2 SH2DepositorInserttcTRP24sub8_SH2sub6
Tags6xHisExpressionBacterialAvailable SinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
tcTRP9sub3
Plasmid#175794Purposede novo thick circular tandem repeat protein trimer formed from 3-repeat monomersDepositorInserttcTRP9sub3
Tags6xHisExpressionBacterialAvailable SinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
tcTRP24sub6
Plasmid#175795Purposede novo thick circular tandem repeat protein tetramer formed from 6-repeat monomersDepositorInserttcTRP24sub6
Tags6xHisExpressionBacterialAvailable SinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Bs-4
Plasmid#163142PurposepLyGo cloning vector for a sequence of interest (LPMO) in B. subtilis. Vector encoding the BatLPMO10 signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterPamyL-PamyQ-PcryIIIA-cryIIIAstab49Available SinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-4
Plasmid#163130PurposepLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PhoA signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterT7Available SinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Bs-3
Plasmid#163141PurposepLyGo cloning vector for a sequence of interest (LPMO) in B. subtilis. Vector encoding the AprE signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterPamyL-PamyQ-PcryIIIA-cryIIIAstab49Available SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Bs-2
Plasmid#163140PurposepLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyQ signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterPamyL-PamyQ-PcryIIIA-cryIIIAstab49Available SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-3
Plasmid#163129PurposepLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding OmpA signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterT7Available SinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-5
Plasmid#163131PurposepLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding DsBA signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterT7Available SinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-optimizedNedd4.2F823D
Plasmid#165105Purposebacterial expression of a GST fusion of Human Nedd4.2d F823DDepositorInsertNedd4.2d F823D (NEDD4L Human)
TagsGST from plasmid.ExpressionBacterialMutationcodon optimized for E. coli expression; F823D; …Available SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA-DAB1
Plasmid#162691PurposeExpresses DabAB1 inorganic carbon transport complexDepositorInsertDabAB1 inorganic carbon transport complex
ExpressionBacterialPromoterPLtet0-1 promoterAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-Myosin7B-sgRNA
Plasmid#154861PurposeLentiviral expression of Cas9 and sgRNA targeting Myosin7BDepositorAvailable SinceJuly 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
sg2
Plasmid#113967PurposeSingle short guide RNA targeting TACCACATTTGTAGAGGTTDepositorInsertsg2
ExpressionMammalianPromoterU6Available SinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
sg3
Plasmid#113968PurposeSingle short guide RNA targeting CAATGTATCTTATCATGTCDepositorInsertsg3
ExpressionMammalianPromoterU6Available SinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
sg2+sg3
Plasmid#113970PurposeDouble short guide RNA targeting TACCACATTTGTAGAGGTT & CAATGTATCTTATCATGTCDepositorInsertsg2+sg3
ExpressionMammalianPromoterU6Available SinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046774
Plasmid#145574PurposeBacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymeraseDepositorInsertSARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;FLAG_2ExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046846
Plasmid#145575PurposeBacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymeraseDepositorInsertSARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;HAExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBMN-AS-FOXA1
Plasmid#139304PurposeKnocking down of human FOXA1 through shRNA and amiRNA targeting FOXA1DepositorInsertshRNA and amiRNA against FOXA1
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pY71-tet.I.-1
Plasmid#133539PurposesfGFP driven by a T7 promoter with the tetO sequence 36 bp upstream from the promoter start siteDepositorInsertsfGFP
TagsStrep tag and TEV siteExpressionBacterialMutationWTPromoterT7Available SinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pY71-tet.I.25
Plasmid#133538PurposesfGFP driven by a T7 promoter with the tetO sequence 28 bp downstream from the promoter start siteDepositorInsertsfGFP
TagsStrep tag and TEV siteExpressionBacterialMutationWTPromoterT7Available SinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pY71-tet.I.20
Plasmid#133537PurposesfGFP driven by a T7 promoter with the tetO sequence 23 bp downstream from the promoter start siteDepositorInsertsfGFP
TagsStrep tag and TEV siteExpressionBacterialMutationWTPromoterT7Available SinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only