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We narrowed to 2,246 results for: Xpa

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Showing: 2141 - 2160 of 2246 results

  1. pDule2-pCNF

    Plasmid
    #85495
    Purpose
    Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.
    Depositor
    Ryan Mehl
    Article
    Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62
    Insert
    para-cyanophenylalanine Mj synthetase
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32L L65V F108W Q109M D158G I159P
    Promoter
    lpp (constitutive)
    Available Since
    Feb. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    85495-plasmid-map-sequence-id-172459

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  2. pEVOL-NBK-1

    Plasmid
    #207639
    Purpose
    A plasmid for the expression of M. mazei pyrrolysine aminoacyl-tRNA synthetase/tRNA pair (PylRS/tRNAPyl) in E. Coli.
    Depositor
    Cole DeForest
    Article
    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1
    Insert
    Pyl-tRNACUA and 2x pyrrolysyl-tRNA synthetase (MmPylRS)
    Expression
    Bacterial
    Mutation
    The evolved synthetase NBK-1 contains mutations Y…
    Promoter
    araBAD
    Available Since
    Oct. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    207639-plasmid-map-sequence-id-409890

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  3. pUltra-sY

    Plasmid
    #82417
    Purpose
    Expresses Mj aaRS for sulfotyrosine and Mj tRNA for decoding UAG codons
    Depositor
    Chang Liu
    Article
    Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542
    Insert
    Methanococcus jannaschii aaRS for sulfotyrosine
    Expression
    Bacterial
    Mutation
    Tyr32Leu, Leu65Pro, Asp158Gly, Ile159Cys, Leu162L…
    Promoter
    tacI
    Available Since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    82417-plasmid-map-sequence-id-156040

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  4. pRSF-G1TFAKRS

    Plasmid
    #177311
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of N6-(trifluoroacetyl)-L-lysine (TFA-Lys) into proteins in E. coli in response to the amber (TAG) codon
    Depositor
    Thomas Huber
    Article
    Orton et al J Am Chem Soc. 2021 Nov 15. doi: 10.1021
    Insert
    tRNA synthetase
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    177311-plasmid-map-sequence-id-348119

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  5. pDule-pCNF

    Plasmid
    #85494
    Purpose
    Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.
    Depositor
    Ryan Mehl
    Article
    Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62
    Insert
    para-cyanophenylalanine Mj synthetase
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32L L65V F108W Q109M D158G I159P
    Promoter
    lpp (constitutive)
    Available Since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85494-plasmid-map-sequence-id-169861

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  7. pDule2-Acd82

    Plasmid
    #174100
    Purpose
    Acridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.
    Depositor
    Ryan Mehl
    Article
    Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964.
    Insert
    Acridone 82 tRNA synthetase and tRNA
    Expression
    Bacterial
    Mutation
    N311S, C313G, V366A, W382T
    Available Since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    174100-plasmid-map-sequence-id-345717

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  10. xCas9

    Plasmid
    #138565
    Purpose
    Expresses human codon-optimized expanded PAM SpCas9 variant and blasticidin resistance: EFS promoter-xCas9-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    xCas9
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    A262T, R324L, S409I, E480K, E543D, M694I, E1219V
    Promoter
    EFS
    Available Since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138565-plasmid-map-sequence-id-274797

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  11. pDule2-para-aminoPhe

    Plasmid
    #85503
    Purpose
    Plasmid for incorporating the non-canonical amino acid para-aminoPhe with the Mj pAF synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Mehl et al J Am Chem Soc. 2003 Jan 29;125(4):935-9.
    Insert
    p-aminoPhe tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32T, E107T, D158P, I159L, L162A
    Promoter
    lpp (constitutive)
    Available Since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85503-plasmid-map-sequence-id-169859

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  15. pDule-Acd82

    Plasmid
    #174099
    Purpose
    Acridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.
    Depositor
    Ryan Mehl
    Article
    Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964.
    Insert
    Acridone 82 tRNA synthetase and tRNA
    Expression
    Bacterial
    Mutation
    N311S, C313G, V366A, W382T
    Available Since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    174099-plasmid-map-sequence-id-345716

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  17. pEVOL-AcKRS-CloDF

    Plasmid
    #127415
    Purpose
    Mutant of mmPylRS designed for incorporation of non-canonical amino acids (acyl-lysine derivatives) in to M13 bacteriophage
    Depositor
    Wenshe Liu
    Article
    Wang et al Angew Chem Int Ed Engl. 2019 Oct 28;58(4
    Insert
    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    Expression
    Bacterial
    Promoter
    araBAD
    Available Since
    April 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    127415-plasmid-map-sequence-id-250212

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  18. pDule2-IBBN (G2)

    Plasmid
    #85501
    Purpose
    Plasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Peeler et al J Am Chem Soc. 2010 Oct 6;132(39):13575-
    Insert
    IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Expression
    Bacterial
    Mutation
    Y32G L65E F108W Q109M D158S R162K
    Promoter
    lpp (constitutive)
    Available Since
    March 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    85501-plasmid-map-sequence-id-169858

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  19. pXPR-SF61

    Plasmid
    #163916
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of para-Pentafluorosulfanyl-Phenylalanine, into proteins in E. coli in response to the amber (TAG) codon
    Depositor
    Thomas Huber
    Article
    Qianzhu et al J Am Chem Soc. 2020 Oct 14;142(41):17277
    Insert
    SF5Phe tRNA synthetase
    Expression
    Bacterial
    Promoter
    araBAD
    Available Since
    March 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    163916-plasmid-map-sequence-id-327914

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Showing: 2141 - 2160 of 2246 results