159,238 results

1. pCMV-PfV-Sapphire-IRES-DSRed

   Plasmid

   #116934

   Purpose

   Mammalian lenti vector for expression of the 40nm GEM and DSRed

   Depositor

   Liam Holt

   Article

   Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

   Insert

   Pfv
   

   Use

   Lentiviral

   Tags

   Sapphire

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDESTsplice

   Plasmid

   #32484

   Depositor

   Stefan Stamm

   Article

   Kishore et al Gene. 2008 Dec 31;427(1-2):104-10. Epub

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQFmcs-2H12.D11

   Plasmid

   #221183

   Purpose

   cdGreen2 expressed from a cumate-inducible promoter (Internal Lab ID: UJ11216)

   Depositor

   Urs Jenal

   Article

   Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

   Insert

   cdGreen2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PQ5 (cumate-inducible)

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. hAAVS1 TALEN Right

   Plasmid

   #52342

   Purpose

   TALEN vector for genomic targeting

   Depositor

   Su-Chun Zhang

   Article

   Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

   Insert

   AAVS1 TALEN Right
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-N2cG

   Plasmid

   #100811

   Purpose

   express rabies virus CVS N2c strain glycoprotein gene (N2cG)

   Depositor

   Ian Wickersham

   Insert

   rabies virus CVS N2c strain glycoprotein gene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-neo-CD2-FAK

   Plasmid

   #37013

   Depositor

   Bob Weinberg

   Article

   Shibue et al Proc Natl Acad Sci U S A. 2009 Jun 23;10

   Insert

   CD2-FAK (CD2, PTK2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEMS-II-PEST

   Plasmid

   #204766

   Purpose

   GEMS 2.0 sensor system with PEST destabilization domain (pCMV-EGFP-PEST-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577+PEST)

   Depositor

   Kate Meyer

   Article

   Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   EGFP-PEST-m6Alinker-DHFR
   

   Use

   Tags

   Expression

   Mutation

   mutated all RACs in EGFP to RGC/RCC

   Promoter

   CMV

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZac2.1-GfaABC1D-mCherry-hPMCA2w/b

   Plasmid

   #111568

   Purpose

   Expresses a plasma membrane calcium pump hPMCA2w/b

   Depositor

   Baljit Khakh

   Article

   Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

   Has Service

   AAV5

   Insert

   Plasma membrane calcium pump 2, variant w/b (ATP2B2 Human)
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPV-Dual_promoter-EF1α-2xNLS-Cascade+Cas3-P (RD)

   Plasmid

   #204619

   Purpose

   Expression vector of Cascade and Cas3. Puromycin selectable.

   Depositor

   Akitsu Hotta

   Article

   Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

   Insert

   Cas7, Cas5, Cas8, Cas11, Cas6, and Cas3
   

   Use

   CRISPR

   Tags

   Each protein has C- and N-terminal SV40 NLS

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCX-SpiCee

    Plasmid

    #140836

    Purpose

    mammalian expression of SpiCee, a buffer of calcium that prevents downstream signaling

    Depositor

    Xavier Nicol

    Article

    Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

    Insert

    SpiCee
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. tau/pET29b

    Plasmid

    #16316

    Depositor

    Peter Klein

    Article

    Hedgepeth et al Dev Biol. 1997 May 1. 185(1):82-91.

    Insert

    tau (MAPT Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3 APEX2-NES

    Plasmid

    #49386

    Purpose

    Expresses APEX2 in the cytosol of mammalian cells

    Depositor

    Alice Ting

    Article

    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

    Insert

    Flag - APEX2 - NES
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K14D, W41F, E112K, A134P on soybean APX; improved…

    Promoter

    CMV

    Available since

    Nov. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pICH47742::p35S-PE2max-NC::tEURb7

    Plasmid

    #227693

    Purpose

    Golden Gate level 1, position 2, PE2max-NC expression cassette.

    Depositor

    Jae-Yean Kim

    Article

    Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

    Insert

    PE2max-NC being driven by long CaMV 35S (p35S) and terminated by a dual terminator (EURb7).
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    Nov. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTipi2.1_Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit HC

    Plasmid

    #221359

    Purpose

    Mammalian expression of Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit HC; to be used with Anti-V5 [IPI-SV5-Pk1] light chain (Plasmid 221360) to make the antibody.

    Depositor

    Institute for Protein Innovation (IPI)

    Insert

    Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit HC
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pCDH-3xFLAG-TERT

    Plasmid

    #51631

    Purpose

    Mammalian expression of 3xFLAG tagged TERT, N-terminal.

    Depositor

    Steven Artandi

    Article

    Zhong et al Cell. 2012 Aug 3;150(3):481-94. doi: 10.

    Insert

    TERT (TERT Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. mito-V5-APEX2

    Plasmid

    #72480

    Purpose

    Expresses APEX2 in mitochondria

    Depositor

    Alice Ting

    Article

    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

    Insert

    APEX2
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    April 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. AICSDP-8:TOMM20-mEGFP

    Plasmid

    #87423

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human TOMM20

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

    Insert

    TOMM20 Homology Arms with linker-mEGFP (TOMM20 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    March 15, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. NES-EGFP-PH-ARNO2G-I303Ex2

    Plasmid

    #116868

    Purpose

    PIP3 biosensor

    Depositor

    Gerry Hammond

    Article

    Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

    Insert

    CYTH2 (CYTH2 Human)
    

    Use

    Tags

    EGFP and X.leavis map2k1.L(32-44)

    Expression

    Mammalian

    Mutation

    amind acids 252-399, isoleucine 303 changed to gl…

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. TurboID-CRaf

    Plasmid

    #226168

    Purpose

    Lentiviral plasmid used to express a TurboID fusion of the protein kinase CRaf.

    Depositor

    Dustin Maly

    Article

    Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

    Insert

    Myc-TurboID-CRaf (RAF1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP-IRES-BlastR and Myc-TurboID

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. MAVS-WT

    Plasmid

    #52135

    Purpose

    Retroviral vector for expression of human MAVS in mammalian cells

    Depositor

    Jonathan Kagan

    Article

    Dixit et al Cell. 2010 May 14;141(4):668-81. Epub 20

    Insert

    MAVS (MAVS Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge