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We narrowed to 13,195 results for: 109

Showing: 5351 - 5400 of 13195 results

  7. pD649-HAsp-IGDCC4-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #157005
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    IGDCC4 (IGDCC4 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    157005-plasmid-map-sequence-id-401035

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  14. pDONR221-SLC27A5_STOP

    Plasmid
    #161270
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    Giulio Superti-Furga, RESOLUTE Consortium
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SLC27A5 (SLC27A5 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 15, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161270-plasmid-map-sequence-id-388958

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  18. pSc1-puro

    Plasmid
    #80438
    Purpose
    Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell, and also confers resistance to puromycin
    Depositor
    Ervin Welker
    Article
    Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare
    Inserts
    sp Cas9 gRNA
    eGFP
    Puromycin resistance
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, U6, and hPGK
    Available since
    Sept. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    80438-plasmid-map-sequence-id-151453

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  26. Desmoplakin II donor only control (F40-based no force control)

    Plasmid
    #118718
    Purpose
    The donor (YPet(short)) only control for the no force control of the F40-based human desmoplakin II tension sensor provides the donor only lifetime used in lifetime measurements to determine FRET.
    Depositor
    Carsten Grashoff
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin II (1-1353)-[YPet(short)-F40-mCherry(Y72L)] (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-F40-mCherry(Y72L)
    Expression
    Mammalian
    Mutation
    truncation after aa1353; Y72L mutation in mCherry…
    Promoter
    CMV
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    118718-plasmid-map-sequence-id-230460

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  28. Desmoplakin II donor only control (FL-based tension sensor)

    Plasmid
    #118723
    Purpose
    The donor only (YPet(short)) control for the FL-based human desmoplakin II tension sensor provides the donor only lifetime used in fluorescence lifetime measurements to determine FRET.
    Depositor
    Carsten Grashoff
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin II-[YPet(short)-FL-mCherry(Y72L)] (internal-1353) (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-FL-mCherry(Y72L)
    Expression
    Mammalian
    Mutation
    inserted FL-based tension sensor module after aa1…
    Promoter
    CMV
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    118723-plasmid-map-sequence-id-230442

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  35. pGEX-5X-1-GST-Armadillo-delta4-6

    Plasmid
    #242226
    Purpose
    Expression of GST-tagged human β-catenin Armadillo repeats ∆4-6 without IDRs (deletion of a.a. 2-133, 268-394, and 672-781) in E. coli
    Depositor
    Courtney Hodges
    Article
    Chan et al Mol Cell. 2025 Jul 15:S1097-2765(25)0057
    Insert
    CTNNB1 (CTNNB1 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Deleted N terminal IDR, arm 4-6, and C terminal I…
    Promoter
    tac
    Available since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    242226-plasmid-map-sequence-id-481884

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  38. pMRX-IP-EGFP-PA-BAX(ER, G108V)

    Plasmid
    #233344
    Purpose
    Stable expression of EGFP-LOV2(N538E)-BAX(G108V)-Cyb5a in mammalian cells. The G108V mutation suppresses pore-forming activity of BAX.
    Depositor
    Noboru Mizushima
    Article
    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.
    Inserts
    LOV2 domain of phototropin1
    BAX (BAX Human)
    Cyb5a (Cyb5a Rat)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    BAX(3-171), N-terminus and C-terminus are deleted…
    Promoter
    Available since
    June 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    233344-plasmid-map-sequence-id-474567

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  41. MKC89_CCR5(UbC-tEPOR-2A-YFP)

    Plasmid
    #232404
    Purpose
    AAV production plasmid for UbC-tEPOR-2A-YFP vector from Fig. 2 that mediates HDR at CCR5 locus using CCR5-sg3 gRNA. YFP is followed by a BGH polyA. AAV genome is flanked by AAV2 ITRs.
    Depositor
    Matthew Porteus, Kyle Cromer
    Article
    Luna et al Nat Biomed Eng. 2024 Dec;8(12):1540-1552
    Insert
    Truncated Erythropoietin Receptor (EPOR Human)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    232404-plasmid-map-sequence-id-462769

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  42. MKC91_tEPOR(UbC-GFP-BGH)

    Plasmid
    #232406
    Purpose
    AAV production plasmid for tEPOR-UbC-GFP-BGH vector from Fig. 1 that mediates HDR at EPOR locus using EPOR-sg1 gRNA. Repair vector introduces premature stop codon into EPOR locus.
    Depositor
    Matthew Porteus, Kyle Cromer
    Article
    Luna et al Nat Biomed Eng. 2024 Dec;8(12):1540-1552
    Insert
    Truncated Erythropoietin Receptor (EPOR Human)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    232406-plasmid-map-sequence-id-462749

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  45. pcs2-MTSmut-cox8A(XXXX)-GFP-IRES-mCherry

    Plasmid
    #226104
    Purpose
    Stability reporter construct of the COX8A mitochondrial targeting sequence (MTS) with mutations that impair recognition by SIFI for transient expression in mammalian cells
    Depositor
    Michael Rape
    Article
    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:
    Insert
    COX8A (COX8A Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    encodes only for mitochondrial targeting sequence…
    Promoter
    Available since
    Oct. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    226104-plasmid-map-sequence-id-448409

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  46. Anti-Shank2 [N23B/6R-2b]

    Plasmid
    #219475
    Purpose
    Mammalian Expression Plasmid of anti-Shank2 scaffold protein (Rat) subclass-switched IgG2b R-mAb. Derived from hybridoma N23B/6.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    Anti-Shank2 scaffold protein (Rattus norvegicus) recombinant mouse monoclonal antibody. (Shank2 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    219475-plasmid-map-sequence-id-430672

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Showing: 5351 - 5400 of 13195 results