We narrowed to 7,680 results for: CCH

Showing: 5351 - 5400 of 7680 results

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pSP571

Plasmid

#139498

Purpose

CRISPR-Cas9 plasmid to generate double strand break in STL1 locus in S. cerevisiae. Expresses both Cas9 and STL1 sgRNA

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pGPD Cas9 / sgRNA (STL162)

Expression

Yeast

Mutation

WT

Promoter

pGPD

Available Since

June 12, 2020

Availability

Academic Institutions and Nonprofits only

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S3_004_026_MV1

Plasmid

#134416

Purpose

pYTKMV1-pTDH3_FdeR-N_2NLS_tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Article

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGMP1 and pTDH3

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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A102-GAP-BoCCS-GRM

Plasmid

#123286

Purpose

Expression of Glo3 ΔAmpH (1-459) in E.Coli

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

ADP-ribosylation factor GTPase-activating protein (GLO3 Budding Yeast)

Tags

His and MBP

Expression

Bacterial

Promoter

T5

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCFD3-MS2tail-Scaffold

Plasmid

#78906

Purpose

Vector for expressing a single sgRNA with Scaffold MS2 tail, under U6:3 promoter

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Type

Empty backbone

Use

CRISPR

Expression

Insect

Available Since

Sept. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pPROEX Hsp104 (773-908)

Plasmid

#1316

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 (773-908) (HSP104 Budding Yeast)

Tags

6X His + TEV cleavage

Expression

Bacterial

Mutation

deletion of aa1-773

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pPROEX Hsp104 (361-548)

Plasmid

#1317

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 (361-548) (HSP104 Budding Yeast)

Tags

6X His + TEV cleavage

Expression

Bacterial

Mutation

aa361-548 of Hsp104

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pES1002

Plasmid

#21789

Depositor

Richard Yu

Article

Yu et al Nature. 2008 Dec 11. 456(7223):755-61.

Insert

Fus3-as2 (FUS3 Budding Yeast)

Expression

Yeast

Mutation

contains 5' 1kb upstream UTR/promoter Q93…

Available Since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only

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pBabe-N-ZAP-Zeo

Plasmid

#17382

Depositor

Stephen Goff

Article

Gao et al Science. 2002 Sep 6. 297(5587):1703-6.

Insert

NZAP (Zc3hav1 Rat)

Use

Retroviral

Expression

Mammalian

Mutation

N-terminal region of ZAP (amino acids 1-254)

Available Since

March 1, 2010

Availability

Academic Institutions and Nonprofits only

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pFUPI AoT5Cyp

Plasmid

#80280

Purpose

Expresses Aotus TRIM5 CypA fusion

Depositor

Jeremy Luban

Article

Neagu et al J Clin Invest. 2009 Oct;119(10):3035-47.

Insert

TRIM5 CypA fusion

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pTH651-RLuc/minFLuc

Plasmid

#29697

Depositor

Tobias von der Haar

Article

Chu et al Nucleic Acids Res. 2011 Aug;39(15):6705-

Insert

Firefly Luciferase

Expression

Yeast

Mutation

All codons of the original FLuc gene were exchang…

Available Since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pGADT7 Hsh155 Y292C

Plasmid

#87291

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 Y292C

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Tags

GAL4 AD and HA

Expression

Bacterial and Yeast

Mutation

Y292C

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pJLI-SUP35 (delta)2-5

Plasmid

#1138

Depositor

Susan Lindquist

Article

Liu et al Nature 1999 Aug 5;400(6744):573-6.

Insert

sup35 delta 2-5 mutant, 5' and 3' flanking regions (SUP35 Budding Yeast)

Expression

Yeast

Mutation

last four repeats of sup35 N domain deleted, but …

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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JBEI-6822

Plasmid

#84985

Purpose

For expression of pBbA5c-MevTaco-MKco-PMKco(aka 2A) under lacUV5 promoter for isopentenol production

Depositor

Taek Soon Lee

Article

George et al Sci Rep. 2015 Jun 8;5:11128. doi: 10.103

Insert

MevTaco-MKco-PMKco

Expression

Bacterial

Promoter

LacUV5

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCfB4613

Plasmid

#126752

Purpose

Integrate pTRX2_5xUAS-CDC14-tADH1 into yeast genome EasyClone site X-4 with SpHIS5 marker

Depositor

Michael Jensen

Article

Zhang et al ACS Synth Biol. 2016 Dec 16;5(12):1546-1

Insert

CDC14 (CDC14 Budding Yeast)

Use

Integrative plasmid

Expression

Yeast

Promoter

pTRX2_5xUAS

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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p416TEF-NQYFP

Plasmid

#99552

Purpose

Expresses Swi1 NQ-YFP to monitor Swi1 state or induce [SWI+].

Depositor

Liming Li

Article

Du et al Mol Cell Biol. 2010 Oct;30(19):4644-55.

Insert

Swi1 NQ-rich region (AA1-536) (SWI1 Budding Yeast)

Tags

YFP

Expression

Yeast

Promoter

TEF1

Available Since

Aug. 30, 2017

Availability

Academic Institutions and Nonprofits only

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HCKan-O-ycrtYB

Plasmid

#65346

Purpose

construction of ycrtYB gene in HCKan-O receiving vector

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

ycrtYB

Use

Synthetic Biology

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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HCKan-O-ycrtE

Plasmid

#65344

Purpose

construction of ycrtE gene in HCKan-O receiving vector

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

ycrtE

Use

Synthetic Biology

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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HCKan-O-ycrtI

Plasmid

#65345

Purpose

construction of ycrtI gene in HCKan-O receiving vector

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

ycrtI

Use

Synthetic Biology

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pSL14 - Prp8 2142–2398

Plasmid

#90264

Purpose

protein expression

Depositor

Christine Guthrie

Article

Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

Insert

PRP8 (PRP8 Budding Yeast)

Tags

N-terminal 6xHis tag

Expression

Bacterial

Mutation

only Jab1 domain (aa 2143-2398)

Promoter

T7

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pRPB1-CTD8

Plasmid

#112820

Purpose

pSMF2 with 8 CTD repeats

Depositor

Stephen Fuchs

Article

Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

Insert

RPB1 (RPO21 Budding Yeast)

Expression

Yeast

Mutation

CTD repeats have identical sequence, deleted repe…

Promoter

Native promoter

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pRPB1-CTD10

Plasmid

#112821

Purpose

pSMF2 with 10 CTD repeats

Depositor

Stephen Fuchs

Article

Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

Insert

RPB1 (RPO21 Budding Yeast)

Expression

Yeast

Mutation

CTD repeats have identical sequence, deleted repe…

Promoter

Native promoter

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P3nmt1-mEGFP

Plasmid

#105137

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Laporte et al Mol Biol Cell. 2012 Aug;23(16):3094-110.

Insert

kanMX6-P3nmt1-mEGFP

Tags

3nmt1-mEGFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

3nmt1

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pETM6-Pc4CL-PhCHS-CmCHI

Plasmid

#73397

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

Pc4CL
PhCHS
CmCHI

Expression

Bacterial

Promoter

T7

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pFP140

Plasmid

#21672

Depositor

James Haber

Article

Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

Insert

Lacz (lacZ E. coli)

Use

Centromeric plasmid

Mutation

The second lacZ sequence has been modified, the E…

Available Since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
pFP120

Plasmid

#21673

Depositor

James Haber

Article

Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

Insert

LacZ (lacZ E. coli)

Use

Centromeric plasmid

Mutation

The second lacZ sequence has been modified, a 918…

Available Since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
pKF251

Plasmid

#52532

Purpose

Double-GFP reporter for DNA cleavage

Depositor

Klaus Foerstemann

Article

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Insert

Double-GFP

Expression

Insect

Promoter

ubiquitin

Available Since

April 25, 2014

Availability

Academic Institutions and Nonprofits only

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pJT3

Plasmid

#98304

Purpose

Overexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of CUP1 promoter

Depositor

Claudia Vickers

Article

Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

Insert

TRPL3AcNES1>TRPL41B

Expression

Yeast

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pNB775

Plasmid

#60115

Purpose

Yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA)

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

YeLuc

Use

Unspecified

Tags

ClpXP degron and Nuclear localization signal (NLS…

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pNB776

Plasmid

#60117

Purpose

Red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA)

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

RdLuc

Use

Unspecified

Tags

ClpXP degron and Nuclear localization signal (NLS…

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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Prp2-pRSETA

Plasmid

#61042

Purpose

Encodes the yeast Prp2 protein for overexpression and purification using a C-terminal hexahistidine tag.

Depositor

Nils Walter

Article

Krishnan et al Nat Struct Mol Biol. 2013 Dec;20(12):145

Insert

Prp2

Tags

Histidine Tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
Human NCKX2-AS-short-FlagTag (pcDNA3.1+)

Plasmid

#75202

Purpose

Mammalian expression of SLC24A2

Depositor

Jonathan Lytton

Article

Visser et al J Biol Chem. 2007 Feb 16;282(7):4453-62.

Insert

SLC24A2 (SLC24A2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

alternatively spliced exon absent

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3_3_TOPO_TA-ms2-VP64

Plasmid

#79371

Purpose

Recruits VP64 to a compatible gRNA for transcriptional activation

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

MS2-VP64

Use

CRISPR

Expression

Mammalian

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pAct:MCP-VP64

Plasmid

#78904

Purpose

Expresses MCP:VP64 under actin promoter for "Scaffold" CRISPRa in Drosophila cells

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

MS2:VP64

Use

CRISPR

Expression

Insect

Promoter

pActin (Drosophila)

Available Since

March 25, 2017

Availability

Academic Institutions and Nonprofits only

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pGS1044

Plasmid

#63797

Purpose

Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal 3xFLAG tag and a LEU2 selection marker

Depositor

Monika Golas, Bjoern Sander

Article

Lin et al Biochim Biophys Acta. 2015 Mar;1854(3):1

Insert

3xFLAG followed by KlLEU2

Use

E. coli cloning vector

Tags

3xFLAG

Promoter

none

Available Since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
YCplac111-KOG1 (pAN58)

Plasmid

#39209

Depositor

Michael Hall

Article

Loewith et al Mol Cell. 2002 Sep;10(3):457-68.

Insert

KOG1 (KOG1 Budding Yeast)

Tags

myc

Expression

Yeast

Mutation

L1512P

Promoter

KOG1

Available Since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only

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p416GAL1-Ssk1t-L-IFP-F[1] (Yeast)

Plasmid

#52897

Purpose

Ssk1t replaces the zipper and is fused to the IFP-F[1] through the linker (GGGGS) X 2. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

Ssk1t-L-IFP-F[1]

Tags

L-IFP-F[1]

Expression

Yeast

Promoter

GAL1

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only
pFP118

Plasmid

#21671

Depositor

James Haber

Article

Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

Insert

Lacz (lacZ E. coli)

Use

Centromeric plasmid

Mutation

The second lacZ sequence has been modified, the E…

Available Since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
pRY1001

Plasmid

#21787

Depositor

Richard Yu

Article

Yu et al Nature. 2008 Dec 11. 456(7223):755-61.

Insert

CFP-Ste12 (STE12 Budding Yeast, A. victoria)

Tags

CFP

Expression

Yeast

Mutation

contains 1000 bp UTR (Ste12 promoter) upstream of…

Available Since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only

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pR-Avr

Plasmid

#22650

Depositor

Lynn Hudson

Article

Kim et al J Neurosci Res. 1997 Oct 15. 50(2):272-9

Insert

Myelin Transcription Factor 1 (Myt1 Rat)

Use

Cloning and rna synthesis

Available Since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only
pmMyt1

Plasmid

#22713

Depositor

Lynn Hudson

Article

Kim et al J Neurosci Res. 1997 Oct 15. 50(2):272-9

Insert

Myelin Transcription Factor 1 (Myt1 Mouse)

Use

Cloning vector tt/aa

Available Since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pYO286

Plasmid

#235730

Purpose

Protein expression of yeast VPS15 (1-981)-ZZ in yeast

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

VPS15

Tags

3xTEV-ZZ

Expression

Yeast

Mutation

aa 1-981

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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SP_H3

Plasmid

#228189

Purpose

Plasmid contains the secretion signal peptide sequence of the yeast CIS3 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

sp_P47001_CIS3_YEAST:0-21

Use

Synthetic Biology

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
SP_F2

Plasmid

#228176

Purpose

Plasmid contains the secretion signal peptide sequence of the yeast EXG1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

sp_P23776_EXG1_YEAST:0-19

Use

Synthetic Biology

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
SP_F3

Plasmid

#228177

Purpose

Plasmid contains the secretion signal peptide sequence of the yeast ECM33 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

sp_B5VE42_ECM33_YEAS6:0-19

Use

Synthetic Biology

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
SP_G1

Plasmid

#228181

Purpose

Plasmid contains the secretion signal peptide sequence of the yeast DSE2 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

sp_P38844_DSE2_YEAST:0-21

Use

Synthetic Biology

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
SP_G2

Plasmid

#228182

Purpose

Plasmid contains the secretion signal peptide sequence of the yeast PRY1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

sp_P47032_PRY1_YEAST:0-19

Use

Synthetic Biology

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
SP_G3

Plasmid

#228183

Purpose

Plasmid contains the secretion signal peptide sequence of the yeast AWA1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

sp_Q8TGE1_AWA1_YEASK:0-23

Use

Synthetic Biology

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
SP_H1

Plasmid

#228187

Purpose

Plasmid contains the secretion signal peptide sequence of the yeast SPR1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

sp_P32603_SPR1_YEAST:0-20

Use

Synthetic Biology

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
SP_H2

Plasmid

#228188

Purpose

Plasmid contains the secretion signal peptide sequence of the yeast HPF1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

sp_Q05164_HPF1_YEAST:0-23

Use

Synthetic Biology

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
SP_D1

Plasmid

#228163

Purpose

Plasmid contains the secretion signal peptide sequence of the yeast MEL2 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

sp_P41945_MEL2_YEASX:0-18

Use

Synthetic Biology

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 7,680 results for: CCH