We narrowed to 12,214 results for: LEA
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Plasmid#134665PurposeExpresses EGFP-tagged peroxisome markerDepositorInserttripeptide SKL (Peroxisome targeting signal 1)
UseLentiviralTagsEGFPExpressionMammalianPromoterEF1aAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
spCas9-NLS-MAV
Plasmid#124212PurposeSpCas9-NLS 17 amino acid linker to monoavidin (SpCas9-NLS-MAV)DepositorInsertcas9
UseCRISPRTagsHis6, MBP, TEV Cleavage site, and two copies of S…ExpressionBacterialPromoterT7Available sinceJune 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
NES-EGFP-P4Mx1
Plasmid#108121PurposePI4P biosensorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertX.leavis map2k1.L(32-44):EGFP:L. penomophila SidM(546-647)
ExpressionMammalianPromoterCMVAvailable sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBOB-synP-HT
Plasmid#30456DepositorInserthistone GFP
UseLentiviralTagsGFPExpressionMammalianAvailable sinceApril 9, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ACE Reporter (pLenti-CMV-mCherry (+43) -T2A-eGFP)
Plasmid#109428PurposeA lentiviral backbone expressing mCherry with a 43 basepair insert and eGFP off of a CMV promoter. A reporter for both Cas9 nuclease and APOBEC-mediated base-editing activity.DepositorInsertmCherry T2A GFP
UseLentiviralMutationInserted 43 base pairs into mCherry to create rep…PromoterCMVAvailable sinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDS24
Plasmid#58952PurposeEncodes synthetic mitochondrial gene ARG8m in place of COX3 coding sequence. Flanked by mtDNA sequences allowing integration into rho+ mtDNA after transformation.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMitochondrially coded Arg8 protein
MutationCoding sequence synthetic in S. cerevisiae mitoch…Promotern/aAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
YTIP-GFP
Plasmid#42238DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertYTIP-GFP
TagsGFPExpressionMammalianPromoterCMVAvailable sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-HA GCNF
Plasmid#16336DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGCNF (Nr6a1 Mouse)
TagsHAExpressionMammalianAvailable sinceDec. 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046852
Plasmid#145584PurposeBacterial Expression plasmid for SARS-CoV-2 nsp8DepositorInsertSARS-CoV-2 nsp8 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
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BAT-gal
Plasmid#20889DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert7XTCF binding sites, Siamois minimal promoter, NLS-lacz
UseMouse TargetingExpressionMammalianMutation7 multimerized TCF-binding elements cloned upstre…Available sinceMay 7, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p(bhsp68-Cas9)
Plasmid#65959PurposeTribolium basal heat shock promoter driving Cas9DepositorInsertCas9
UseCRISPRTagsnuclear localization sequenceExpressionInsectPromoterbhspAvailable sinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA5-FRT/TO-3*Flag-APEX2 C-term 3NLS
Plasmid#187588PurposeExpress FLAG epitope and APEX2-tagged 3xNLS fusion protein in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNLS
TagsAPEX2-FLAGExpressionMammalianPromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-KSENB-Nup133
Plasmid#87340PurposeTo express human Nup133 in mammalian cellsDepositorInsertNUP133 (NUP133 Human)
TagsEGFPExpressionMammalianMutationdeletion of Nup133 AA1-7PromoterCMVAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBAD24-sfGFPx2
Plasmid#51559PurposeSuperfolder GFP ORF cloned into pBAD24 for expression in E. coli. It contains additional aminoacids in the N and C terminus (polilinker sequences for cloning proteins in frame with GFP)DepositorInsertsuperfolder GFP
TagsGLESTCRHASLAVLADERRFSA and MARARAExpressionBacterialMutationContains additonal aa in the N and C terminus (in…Available sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEJS1096 Dual-sgRNA.Design 1
Plasmid#159538PurposeDelivery of dual sgRNA cassettes and Nme2Cas9 in a single AAV vectorDepositorInsertNme2Cas9 nuclease with two guide RNA cassettes with promoters
UseAAV, CRISPR, and Mouse TargetingTagsNLSExpressionMammalianPromoterU1aAvailable sinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSYN-bArrestin2-TevC-P2A-TdTomato-WPRE-bGHpA
Plasmid#89873Purposeexpresses iTango bArrestin2-TevC component and TdTomato bicistronically for AAV productionDepositorInsertbArrestin2-TevC-P2A-TdTomato
UseAAVTagsTdTomatoExpressionMammalianMutationR173H in TdTomato (Please see depositor comments …PromoterHuman synapsinAvailable sinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMM178
Plasmid#61270PurposeAlso referred to as pRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains a pBBR1 origin and chloramphenicol resistance.DepositorInsertCas9, tracrRNA, crRNA
UseCRISPRTags6xHisExpressionBacterialPromoterpLtetO, native, native (respectively)Available sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
MSCV-HA-Nr4a1
Plasmid#160941PurposeGeneration of retrovirus for the overexpression of Nr4a1DepositorAvailable sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
R26-H2B-EGFP HR donor vector
Plasmid#137925PurposeR0SA26 knock-in vector with homologous recombination-based methodDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSA-H2B-EGFP-bpA with homology arms
UseMouse TargetingAvailable sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by