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pMM131

Plasmid

#127214

Purpose

BeYDV replicon with constitutive Luc expression, AtSTM, sgRNA targeting PDS

Depositor

Daniel Voytas

Publication

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc, AtSTM, sgRNA

Expression

Plant

Mutation

codon optimized CDSs

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pPac Sp1

Plasmid

#12095

Depositor

Robert Tjian

Publication

Courey et al Cell. 1988 Dec 2. 55(5):887-98.

Insert

Sp1 (SP1 Human)

Expression

Insect

Available since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only
pET32bplus_trx_dAChE4

Plasmid

#83917

Purpose

Encodes an E. coli codon optimized version of dAChE4 - a designed variant of human acetylcholinesterase bearing 51 mutations with ~1800 fold higher functional expression levels in E. coli

Depositor

Sarel Fleishman

Publication

Goldenzweig et al Mol Cell. 2016 Jul 21;63(2):337-46. doi:

Insert

Designed hAChE, codon optimised for E.coli expression (ACHE Human, Synthetic)

Tags

His, S-tag, and TRX

Expression

Bacterial

Mutation

V13T,K24T,M43V,L49R,V61W,S68N,E92N,T110K,S111N,T1…

Promoter

T7 promoter

Available since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST26-C-FLAG

Plasmid

#79275

Purpose

Destination vector containing a C-terminal FLAG tag

Depositor

Rita Shiang

Publication

Amr et al Am J Hum Genet. 2007 Oct;81(4):673-83. E

Insert

FLAG

Expression

Mammalian

Promoter

CMV

Available since

March 13, 2017

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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mouse Oct4-GFP GOF18 transgenic reporter

Plasmid

#60527

Purpose

BAC recombineering generated construct, used as a reporter for Oct4 expression (containes both DE and PE elements)

Depositor

Jacob Hanna

Publication

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Insert

GFPpA

Expression

Mammalian

Promoter

Oct4

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pMKV059

Plasmid

#133314

Purpose

BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+ and IPT

Depositor

Daniel Voytas

Publication

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc+, IPT

Expression

Plant

Promoter

AtUbi10, 35S

Available since

Jan. 29, 2020

Availability

Academic Institutions and Nonprofits only

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LeGO-1xT/BSD

Plasmid

#27353

Depositor

Boris Fehse

Publication

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

U6 promoter, SFFV promoter, dTomato/BSD-Fusion

Use

Lentiviral

Expression

Mammalian

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Dlx2

Plasmid

#15537

Depositor

John Rubenstein

Publication

Porteus et al Neuron. 1991 Aug . 7(2):221-9.

Insert

Dlx2 (Dlx2 Mouse)

Expression

Bacterial

Mutation

Partial cDNA

Available since

Aug. 20, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pTriEx-mVenus-PA-Rac1-T17N

Plasmid

#22017

Purpose

Expression of photoactivatable Rac1 (dominant-negative mutant)

Depositor

Klaus Hahn

Publication

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

PA-Rac1-T17N (RAC1 Human, Avena sativa (oat))

Tags

6X His and mVenus

Expression

Bacterial, Insect, and Mamm…

Mutation

Rac1 starts at I4 and contains mutation T17N.

Promoter

CMV, p10

Available since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-Ef1a-FAS-EGFP-WPRE-pA

Plasmid

#37091

Purpose

Expresses EGFP in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites)

Depositor

Bernardo Sabatini

Publication

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

d1eGFP

Use

AAV and Cre/Lox; Cre-off

Promoter

EF1a

Available since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Ad-Flag-XBP1s

Plasmid

#63678

Purpose

Adenoviral vector for XBP1s expression

Depositor

Qingbo Xu, Lingfang Zeng

Publication

Zeng et al Proc Natl Acad Sci U S A. 2009 May 19;10

Insert

XBP1 (XBP1 Human)

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Mutation

spliced

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Degron-KI donor backbone

Plasmid

#65483

Purpose

Donor backbone vector to clone left and right HR arms in for Degron-KI application

Depositor

Frank Stegmeier

Publication

Zhou et al Cancer Res. 2015 Mar 18. pii: canres.293

Type

Empty backbone

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pKS1

Plasmid

#84079

Purpose

Expresses the non-toxic fragment C of tetanus toxin

Depositor

Neil Fairweather

Publication

Sinha et al Mol Microbiol. 2000 Sep;37(5):1041-51.

Insert

Tetanus neurotoxin fragment C

Tags

His tag

Expression

Bacterial

Promoter

T7

Available since

April 21, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET28a MISTIC

Plasmid

#85999

Purpose

bacterial expression of transmembrane proteins using N-terminal MISTIC and C-terminal HIS6 tag

Depositor

Wolfram Antonin

Publication

Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

Insert

N/A

Tags

HIS6 and MISTIC

Expression

Bacterial

Mutation

codon optimzed for bacterial expression

Promoter

T7

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTriEx-mCerulean-PA-Rac1

Plasmid

#22030

Purpose

Expression of photoactivatable Rac1

Depositor

Klaus Hahn

Publication

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

PA-Rac1 (RAC1 Human, Avena sativa (oat))

Tags

6X His and mCerulean

Expression

Bacterial, Insect, and Mamm…

Mutation

Rac1 starts at I4 and contains mutations Q61L, E9…

Promoter

CMV, p10

Available since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pF2-DTA

Plasmid

#42521

Depositor

Terry Magnuson

Publication

Jang et al PLoS One. 2013;8(2):e57075. doi: 10.1371

Insert

EF1a-DTA

Use

Mouse Targeting

Available since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA Set9 mutant

Plasmid

#24085

Depositor

Danny Reinberg

Publication

Nishioka et al Genes Dev. 2002 Feb 15. 16(4):479-89.

Insert

Set9 (SETD7 Human)

Tags

FLAG

Expression

Mammalian

Mutation

H297A

Available since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only
PKT2/CLP-AKT

Plasmid

#20281

Depositor

John Ohlfest

Publication

Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

Insert

AKT (AKT1 Human)

Use

Transposon

Available since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pMM135

Plasmid

#127216

Purpose

BeYDV replicon with constitutive Luc expression, WUS2, sgRNA targeting PDS

Depositor

Daniel Voytas

Publication

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc, WUS2, sgRNA

Expression

Plant

Mutation

codon optimized CDSs

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCas1+2

Plasmid

#72676

Purpose

Express Cas1 and Cas2 for the CRISPR adaptation process in Escherichia coli. Has streptomycin resistance.

Depositor

Udi Qimron

Publication

Yosef et al Nucleic Acids Res. 2012 Jul;40(12):5569-

Insert

Cas1, Cas2

Expression

Bacterial

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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