We narrowed to 2,368 results for: pcas9

1. pJT173_GalL_A3A-R128A-BE3

   Plasmid

   #145122

   Purpose

   Expressing base editor A3A-R128A-BE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   A3A(R128A)-BE3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   A3A(R128A); spCas9(D10A)

   Promoter

   GalL

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJT122_GalL_cCDA1-VQRBE3

   Plasmid

   #145087

   Purpose

   Expressing base editor cCDA1-VQRBE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   cCDA1-VQRBE3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   spCas9(D10A, D1135V, R1335Q, T1337R)

   Promoter

   GalL

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJT113_GalL_cCDA1-xBE3

   Plasmid

   #145079

   Purpose

   Expressing base editor cCDA1-xBE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   cCDA1-xBE3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   spCas9(D10A, A262T, R324L, S409I, E480K, E543D, M…

   Promoter

   GalL

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX335_HR_Prnp_3

   Plasmid

   #78622

   Purpose

   pX335 vector encoding SpCas9n and a chimeric guide RNA targeting Prnp coding sequence

   Depositor

   Walker Jackson

   Article

   Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

   Insert

   HR_Prnp_3 (Prnp Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Aug. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO1-puro-U6-sgRNA-KANK3

   Plasmid

   #69238

   Purpose

   U6 driven SpCas9 sgRNA expression for KANK3 site

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   KANK3 sgRNA (KANK3 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. Px461-Cas9n-Trp53-sgRNA-beta

   Plasmid

   #88847

   Purpose

   CRISPR KO of Trp53

   Depositor

   Joan Massague

   Article

   Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

   Insert

   Trp53 (Trp53 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

7. Px461-Cas9n-Trp53-sgRNA-alpha

   Plasmid

   #88846

   Purpose

   CRISPR KO of Trp53

   Depositor

   Joan Massague

   Article

   Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

   Insert

   Trp53 (Trp53 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

8. pT7-PE7 for IVT

   Plasmid

   #214813

   Purpose

   Template for in vitro transcription of PE7

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7
   

   Use

   Template for in vitro transcription

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mutation

   Promoter

   T7 (inactivated)

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. ABE7.10-F148A

   Plasmid

   #132946

   Purpose

   Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.

   Depositor

   Hui Yang

   Article

   Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:

   Insert

   ABE7.10(F148A)
   

   Use

   CRISPR; Base editor

   Tags

   Expression

   Mammalian

   Mutation

   ABE7.10(F148A)

   Promoter

   Available Since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. T7_CC_PE7_IVT_Template

    Plasmid

    #223022

    Purpose

    Template for in vitro transcription of PE7. For HSPC-related experiments.

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7
    

    Use

    Template for in vitro transcription

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330-PITCh-ACTB

    Plasmid

    #207748

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of ACTB for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of ACTB (ACTB Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. CRISPR-SB

    Plasmid

    #177936

    Purpose

    Expresses SpCas9 and sgRNA. Can be integrated into the genome upon co-delivery of Sleeping Beauty transposase.

    Depositor

    Roland Rad

    Article

    Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

    Type

    Empty backbone

    Use

    CRISPR; Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330-PITCh-LMNB1

    Plasmid

    #207770

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of LMNB1 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of LMNB1 (LMNB1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330-sgR26

    Plasmid

    #127376

    Purpose

    Used for contransfection with pR26- and pR26-CMVconst-derived constructs to mediate CRISPR/Cas9 genomic insertion into the murine ROSA26 safe harbor locus.

    Depositor

    Lance Miller

    Article

    Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1

    Insert

    ROSA26 (Gt(ROSA)26Sor Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV_Cas9–superNLS

    Plasmid

    #232430

    Purpose

    Expression plasmid for the SpCas9 nuclease with superNLS

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

    Insert

    Cas9–superNLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-2NLS-Cas9

    Plasmid

    #229773

    Purpose

    Expresses Cas9 driven by a CMV promoter

    Depositor

    Yujia Cai

    Article

    Ling et al Nat Nanotechnol. 2025 Apr;20(4):543-553.

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. CGBE1-NG (pBM1246)

    Plasmid

    #140256

    Purpose

    CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    eUNG-BE4max(R33A)_NG-ΔUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

    Promoter

    CMV

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pABE8e-NG-403Q-Nterm-AAV

    Plasmid

    #194651

    Purpose

    N-terminal AAV genome plasmid encoding ABE8e + sgRNA to correct the R403Q mutation in mice

    Depositor

    David Liu

    Article

    Reichart et al Nat Med. 2023 Feb;29(2):412-421. doi: 10

    Insert

    ABE8e-NG N-term
    

    Use

    AAV

    Tags

    BPNLS and NpuN spilt intein

    Expression

    Mutation

    Promoter

    TNNT2

    Available Since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pABE8e-NG-403Q-Cterm-AAV

    Plasmid

    #194652

    Purpose

    C-terminal AAV genome plasmid encoding ABE8e + sgRNA to correct the R403Q mutation in mice

    Depositor

    David Liu

    Article

    Reichart et al Nat Med. 2023 Feb;29(2):412-421. doi: 10

    Insert

    ABE8e-NG C-term
    

    Use

    AAV

    Tags

    BPNLS and NpuC split intein

    Expression

    Mutation

    Promoter

    TNNT2

    Available Since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. EF1a-TET1-dCas9

    Plasmid

    #235593

    Purpose

    Tet1CD fused to N-terminus of dCas9

    Depositor

    Ting Zhou

    Article

    Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

    Insert

    TET1-dCas9 (TET1 SpCas9 is from Streptococcus pyogenes, Human)
    

    Use

    CRISPR

    Tags

    3xNLS, TET1 CD, and tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    May 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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