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Showing: 3241 - 3270 of 16022 results

  2. AAV2-ITR-pEFS-NLS_hfCas13d(RfxCas13d_N2V8)_FLAG_NLS-pA-U6-DR-Pcsk9-sg5-DR-Pcsk9-sg6-DR-AAV2-ITR

    Plasmid
    #191795
    Purpose
    vector for encoding and AAV packaging a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by EFS promoter, Pcsk9-target guide RNAs compatible with RfxCas13d driven by hU6
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103
    Insert
    humanized hfCas13d
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    A134V, A140V, A141V, A143V
    Promoter
    EFS, hU6
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    191795-plasmid-map-sequence-id-375580

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  5. pRL_CT_GI_del1

    Plasmid
    #98744
    Purpose
    For red/far-red light-regulated gene expression in Saccharomyces cerevisiae. Integrates into the yeast ura3-52 locus. Encodes constitutively expressed PhyBNT-synTALE-DBD and PIF3-NLS-VP64AD fusions.
    Depositor
    Bernd Müller-Röber
    Article
    Hochrein et al Nucleic Acids Res. 2017 Sep 6;45(15):919
    Inserts
    phytochrome interacting factor 3 (PIF3 Mustard Weed)
    phytochrome B (PHYB Mustard Weed)
    Use
    Synthetic Biology
    Tags
    SV40-NLS, VP64-AD, and synTALE-DBD
    Expression
    Yeast
    Mutation
    mutated nucleotide 576 C to A, deleted amino acid…
    Promoter
    FBA1 and TDH3
    Available since
    Aug. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98744-plasmid-map-sequence-id-189858

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  7. Anti-Pan-Neurofascin (extracellular) [A12/18R]

    Plasmid
    #177438
    Purpose
    Mammalian Expression Plasmid of anti-Pan-Neurofascin (extracellular) (Rat). Derived from hybridoma A12/18.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    anti-Pan-Neurofascin (extracellular) (Rattus norvegicus) recombinant mouse monoclonal antibody (Nfasc Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Nov. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    177438-plasmid-map-sequence-id-348771

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  8. pAAV-EF1a-iCreV

    Plasmid
    #140135
    Purpose
    Can be used to generate AAV virus that will express light-inducible site-specific iCreV recombinase
    Depositor
    Hongkui Zeng
    Article
    Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s
    Has Service
    AAV PHP.eB and AAV1
    Insert
    iCreV
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1alpha
    Available since
    April 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    140135-plasmid-map-sequence-id-292208

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  9. G protein alpha-s(alpha3beta5/G226A/A366S)

    Plasmid
    #55797
    Purpose
    This highly effective dominant negative G protein alpha-s mutant contains, in addition to the mutations in alpha-s(alpha3beta5/G226A), the A366S mutation, which increases GDP release.
    Depositor
    Catherine Berlot
    Article
    Berlot et al J Biol Chem. 2002 Jun 7;277(23):21080-5.
    Insert
    alpha-s (alpha3beta5/G226A/A366S) (Gnas Rat)
    Use
    Tags
    internal EE epitope (residues 189-194 in alpha-s …
    Expression
    Mammalian
    Mutation
    N271K, K274D, R280K, T284D, I285T, G226A, A366S i…
    Promoter
    CMV
    Available since
    Aug. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    55797-plasmid-map-sequence-id-112181

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  22. Anti-VGlut3 [N34/34R-2b]

    Plasmid
    #219476
    Purpose
    Mammalian Expression Plasmid of anti-VGlut3 neurotransmitter transporter (Rat) subclass-switched IgG2b R-mAb. Derived from hybridoma N34/34.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    Anti-VGlut3 neurotransmitter transporter (Rattus norvegicus) recombinant mouse monoclonal antibody. (Slc17a8 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    219476-plasmid-map-sequence-id-430663

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  23. Anti-VGlut1 [N28/9R-rat IgG2a]

    Plasmid
    #222184
    Purpose
    Mammalian Expression Plasmid of anti-VGlut1 neurotransmitter transporter (Rat). Derived from hybridoma N28/9.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    Anti-VGlut1 neurotransmitter transporter (Rattus norvegicus) recombinant mouse monoclonal antibody. (Slc17a6 Chimera: M. musculus (mouse)/R. norvegicus (rat))
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    222184-plasmid-map-sequence-id-437282

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  24. Anti-VGlut2 [N29/29R-1]

    Plasmid
    #206707
    Purpose
    Mammalian Expression Plasmid of anti-VGlut2 neurotransmitter transporter (Rat). Derived from hybridoma N29/29-1.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    anti-VGlut2 neurotransmitter transporter (Rattus norvegicus) recombinant Mouse monoclonal antibody (Slc17a6 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Nov. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    206707-plasmid-map-sequence-id-414160

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  27. pLH_Scr15

    Plasmid
    #100537
    Purpose
    CEN/ARS plasmid with expression cassettes for PhyBNT-CreN and PIF3-NLS-CreC fusion proteins for red/far-red light-regulated Cre recombinase activity in Saccharomyces cerevisiae.
    Depositor
    Bernd Müller-Röber
    Article
    Hochrein et al Nat Commun. 2018 May 22;9(1):1931. doi:
    Inserts
    Phytochrome B (PHYB Mustard Weed)
    Phytochrome interacting factor 3 (PIF3 Mustard Weed)
    Use
    Cre/Lox and Synthetic Biology
    Tags
    CreC, CreN, and SV40 NLS
    Expression
    Yeast
    Mutation
    N-terminal version of PhyB (AA 1-621)/ Mutation o…
    Promoter
    FBA1 and TDH3
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    100537-plasmid-map-sequence-id-194456

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Showing: 3241 - 3270 of 16022 results