Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 52,823 results for: plasmid

Showing: 5401 - 5450 of 52823 results

  4. AAV-SYN-NLS-SaCas9-NLS-3xHA-bGHpA-U6-BsaI-sgRNA

    Plasmid
    #218710
    Purpose
    AAV vector plasmid expressing Cas9 from Staphylococcus aureus (SaCas9) under the human synapsin (SYN) promoter and its sgRNA under the U6 promoter
    Depositor
    Bart Nieuwenhuis
    Inserts
    hSaCas9
    Chimeric guide for SaCas9
    Use
    AAV and CRISPR
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218710-plasmid-map-sequence-id-433357

    Enlarge

  5. pJAT21

    Plasmid
    #204309
    Purpose
    For generating targeting inversions. N-terminal split GFP, expressed in abdomen and mouthparts
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    ie1-GFPn
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ie1
    Available since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    204309-plasmid-map-sequence-id-405852

    Enlarge

  6. pJAT25

    Plasmid
    #204310
    Purpose
    For generating targeting inversions. C-terminal split GFP, expressed in abdomen and mouthparts.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    ie1-GFPc
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ie1
    Available since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    204310-plasmid-map-sequence-id-405854

    Enlarge

  7. pGBW-m4134100

    Plasmid
    #152050
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;6xHis-003
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    May 20, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152050-plasmid-map-sequence-id-300237

    Enlarge

  10. COVID-SARS2 NSP16/10

    Plasmid
    #159615
    Purpose
    Bacterial co-expression vector fo Covid-SARS2 NSP16 and NSP10
    Depositor
    Opher Gileadi
    Inserts
    Covid-SARS2 Nsp16
    Covid-SARS2 Nsp10
    Use
    Tags
    His6-Zb-TEV and none
    Expression
    Bacterial
    Mutation
    Codon-optimized for E. coli expression
    Promoter
    (Bicistronic) and T7 - LacO
    Available since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    159615-plasmid-map-sequence-id-311066

    Enlarge

  11. pE_Cre_lambdaCos

    Plasmid
    #173785
    Purpose
    The pE_Cre_lambdaCos plasmid is used to remove the antibiotic resistance cassette from pIT5 integrants.
    Depositor
    Keith Shearwin
    Article
    Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102
    Insert
    Cre
    Use
    Cre/Lox and Synthetic Biology
    Tags
    Expression
    Mutation
    S102Y and S279Y- please see depositor comments
    Promoter
    Available since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    173785-plasmid-map-sequence-id-342369

    Enlarge

  13. pJAT27

    Plasmid
    #204308
    Purpose
    For generating targeting inversions. C-terminal split GFP, expressed in flight muscles.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    MHC-GFPc
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    MHC
    Available since
    Oct. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    204308-plasmid-map-sequence-id-411129

    Enlarge

  14. pJAT18

    Plasmid
    #204307
    Purpose
    For generating targeting inversions. N-terminal split GFP, expressed in flight muscles.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    MHC-GFPn
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    MHC
    Available since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    204307-plasmid-map-sequence-id-405848

    Enlarge

  19. pGBW-m4133227

    Plasmid
    #152132
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;3xFlag
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    May 20, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152132-plasmid-map-sequence-id-297953

    Enlarge

  21. pGBW-m4046203

    Plasmid
    #145488
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable thrombin;3xFLAG
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pGAL1
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145488-plasmid-map-sequence-id-306276

    Enlarge

  22. pGBW-m4137386

    Plasmid
    #149540
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Human, Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits

  24. pGBW-m4046233

    Plasmid
    #149032
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 nsp3
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp3 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable thrombin;6xHIS
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pTEF1
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149032-plasmid-map-sequence-id-298060

    Enlarge

  25. pGBW-m4046664

    Plasmid
    #149224
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 E (envelope)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 E (envelope) (E Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pTEF1
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149224-plasmid-map-sequence-id-291677

    Enlarge

  27. pGBW-m4252984

    Plasmid
    #153898
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 SARS-CoV-2 E (envelope)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153898-plasmid-map-sequence-id-300439

    Enlarge

  28. pGBW-m4134900

    Plasmid
    #151911
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 spike prefusion-stabilized
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 spike prefusion-stabilized (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    d1-15 d1209-1273 R682A R685G K986P V987P
    Promoter
    CMV
    Available since
    May 20, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    151911-plasmid-map-sequence-id-300222

    Enlarge

  29. pJAT23

    Plasmid
    #204312
    Purpose
    For generating targeting inversions. C-terminal split GFP, expressed in eyes.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    3XP3-GFPc
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    3XP3
    Available since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    204312-plasmid-map-sequence-id-405857

    Enlarge

  31. pGBW-m4046479

    Plasmid
    #145495
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 nsp4
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp4 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable thrombin;3xFLAG
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pGAL1
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145495-plasmid-map-sequence-id-300257

    Enlarge

  33. pGBW-m4046409

    Plasmid
    #153939
    Purpose
    Yeast expression plasmid for SARS-CoV-2 S (surface glycoprotein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable thrombin;StrepII
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pGAL1
    Available since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153939-plasmid-map-sequence-id-300502

    Enlarge

  34. XX01CAPN1A-c001

    Plasmid
    #175491
    Purpose
    Protein expression in bacterial cells. Entire CDS, M1-A714 (CAPN1); EF hand 1-5, H86-S268 (CAPNS1). CAPN1 can be biotinylated. Codon optimised.
    Depositor
    Opher Gileadi
    Insert
    CAPN1 / CAPNS1 (CAPN1 Human)
    Use
    Tags
    AviTag and His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175491-plasmid-map-sequence-id-353165

    Enlarge

  35. pSCM167.2-Venus-VF-hADAM10

    Plasmid
    #189552
    Purpose
    Protein expression ADAM10 in mammalian cells.
    Depositor
    Haian Fu
    Insert
    ADAM metallopeptidase domain 10 (ADAM10 Human)
    Use
    Tags
    VF
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    189552-plasmid-map-sequence-id-374370

    Enlarge

  36. pGBW-m4046323

    Plasmid
    #149100
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 nsp3
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp3 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable thrombin;MBP
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pGAL1
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149100-plasmid-map-sequence-id-298109

    Enlarge

  38. pGBW-m4046348

    Plasmid
    #149108
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 nsp6
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp6 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable thrombin;3xFLAG
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pGAL1
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149108-plasmid-map-sequence-id-298127

    Enlarge

  39. RAC1 (RAC1A-c007)

    Plasmid
    #110257
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    RAC1 (RAC1 Human)
    Use
    Tags
    StrepII-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    110257-plasmid-map-sequence-id-212328

    Enlarge

  40. pGBW-m4258306

    Plasmid
    #154795
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 E (envelope)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;V5
    Expression
    Mammalian
    Mutation
    H. sapiens recode 1
    Promoter
    CMV
    Available since
    July 30, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154795-plasmid-map-sequence-id-311546

    Enlarge

  41. pGBW-m4252845

    Plasmid
    #153787
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;6xHis-003
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153787-plasmid-map-sequence-id-307368

    Enlarge

  42. pAAV hSyn1 bPAC cMyc S27A 2A tDimer

    Plasmid
    #85398
    Purpose
    humanized photoactivated adenylyl cyclase lower dark activity, spectrum shifted 15 nm longer wavelenght with neuron-specific promoter, plus red fluorescent protein
    Depositor
    Thomas Oertner
    Inserts
    humanized photoactivated adenylyl cyclase
    red fluorescent protein
    Use
    AAV
    Tags
    cMyc Tag
    Expression
    Mammalian
    Mutation
    S27A
    Promoter
    human Synapsin1 promotor and ribosomal skip seque…
    Available since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    85398-plasmid-map-sequence-id-201308

    Enlarge

  43. pMAZ-donor

    Plasmid
    #112359
    Purpose
    CRISPR donor plasmid to tag human transcription factor MAZ with GFP
    Depositor
    Kevin White
    Insert
    MAZ homology arms flanking EGFP-IRES-Neo cassette (MAZ Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:16:29809765:G>A
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112359-plasmid-map-sequence-id-229679

    Enlarge

  44. pGBW-m4046865

    Plasmid
    #153930
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 3'-to-5' exonuclease
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153930-plasmid-map-sequence-id-307315

    Enlarge

  45. pGBW-m4046310

    Plasmid
    #149022
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 M (membrane) (M Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable thrombin;MBP
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pTEF1
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149022-plasmid-map-sequence-id-291475

    Enlarge

  47. pGBW-m4046373

    Plasmid
    #145555
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 ORF3a protein
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 ORF3a protein (ORF3a Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable thrombin;StrepII
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pGAL1
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145555-plasmid-map-sequence-id-384616

    Enlarge

  48. pGBW-m4133326

    Plasmid
    #152113
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;HA
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    May 20, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152113-plasmid-map-sequence-id-297929

    Enlarge

  49. pYCTK019

    Plasmid
    #176723
    Purpose
    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Ste2 gene of Candida albicans (receiver part).
    Depositor
    Victor Sourjik
    Article
    Krink et al bioRxiv 2021
    Insert
    ste2Ca
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Codon-optimized for expression in S. cerevisiae
    Promoter
    Available since
    Jan. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    176723-plasmid-map-sequence-id-351592

    Enlarge

  50. pLP-hSox2-ires-gfp

    Plasmid
    #49391
    Purpose
    mammalian bicistronic expression of human Sox2 and EGFP
    Depositor
    Randall McKinnon
    Insert
    Sox2 (SOX2 Human)
    Use
    Cre/Lox; Acceptor vector
    Tags
    6xHN, EGFP, and IRES
    Expression
    Mammalian
    Mutation
    TGA (stop) to CCA (Pro) at nt 1027 with respect t…
    Promoter
    CMV
    Available since
    Dec. 2, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 5401 - 5450 of 52823 results