We narrowed to 655 results for: Bacillus
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Plasmid#27171DepositorInsertsurface (S)-layer protein promoter region fused with modified green fluorescent protein 5
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTRKH3-slpGFP
Plasmid#27168DepositorInsertsurface (S)-layer protein promoter region fused with modified green fluorescent protein 5
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLCNICK
Plasmid#84653PurposeGenome editing for Lactobacillus casei Lc2WDepositorInsertsCas9 nickase
sgRNA
homology arms of LC2W_2179
UseCRISPRTagsExpressionMutationD10APromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS-ldhGFP
Plasmid#27170DepositorInsertlactate dehydrogenase promoter region fused with modified green fluorescent protein 5
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pG1AK-sfGFP
Plasmid#71739PurposeModular shuttle vector for Geobacillus and E. coliDepositorInsertsfGFP
UseSynthetic Biology; Shuttle vectorTagsExpressionBacterialMutationPromoterpRplsWTAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSL5200 (pDonor_ISGst3)
Plasmid#210875PurposeHarbors a native ISGst3 transposon derived form Geobacillus stearothermophilus DSM 458DepositorInsertISGst3
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLEM415-ldhL-mRFP1
Plasmid#99842PurposeExpression of RFP in LactobacillusDepositorInsertmonomeric red fluorescent protein
UseTagsFLAGExpressionBacterialMutationPromoterldhlAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAFi-dHdrA
Plasmid#184907Purposecarrying CRISPR/dCas9 system for gene knockdown in Acidithiobacillus ferriduransDepositorInsertCm-sgRNA-dCas9
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBz118_His_CsoS2
Plasmid#140841PurposeHalothiobacillus neapolitanus CsoS2 with N-terminal His-tag, pET-14b backboneDepositorInsertCsoS2
UseTagsHexahistidine tagExpressionBacterialMutationPromoterT7Available sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHSP02
Plasmid#117259PurposeGenome editing for Lactobacillus plantarum WCSF1DepositorInsertCas9, promotor P11-guide-RNA, homologous arms of Lp_0537
UseOtherTagsExpressionMutationPromoterAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHSB04X
Plasmid#117258PurposeGenome editing for Lactobacillus brevis ATCC367DepositorInsertCas9, promotor PslpA-guide-RNA, homologous arms of Lb_1019
UseOtherTagsExpressionMutationPromoterAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAF-HdrB3
Plasmid#184908Purposecarrying CRISPR/Cas9 system for genome editing in Acidithiobacillus ferriduransDepositorInsertCas9-sgRNA-homology arms
UseCRISPR; Broad-host-rangeTagsExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLz37_CB_operon_CsoS2_N1-N4
Plasmid#140856PurposeHalothiobacillus neapolitanus carboxysome operon with full length CsoS2. For heterologous expression of carboxysomes in E. coli.DepositorInsertCbbL, CbbS, CsoS2 (full-length), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBz15_Strep_Rubisco
Plasmid#140839Purposewild-type Halothiobacillus neapolitanus Form I Rubisco with StrepII tag (CbbL-StrepII, CbbS), pET-14b backboneDepositorInsertForm IA Rubisco; CbbL, CbbS
UseTagsStrep IIExpressionBacterialMutationPromoterT7Available sinceJune 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC57-LbNOX
Plasmid#75285PurposeThis plasmid contains human codon optimized sequence of LbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.DepositorInsertLbNOX
UseTagsC-terminal FLAG tag with linkerExpressionMutationHuman codon optimizedPromoterAvailable sinceMay 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGDP3 cat
Plasmid#112888PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertcat (Brevibacillus brevis) (cat Brevibacillus brevis)
UseTags6xHisExpressionBacterialMutationPromoterPblaAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUBC-popTag-QtE-mScarlet
Plasmid#240101PurposeMammalian lentiviral vector for expressing the mScarlet-tagged 50nm-GEMs, encoded by the encapsulin protein from Quasibacillus thermotolerans and fused to a PopZ tag.DepositorInsertEncapsulin
UseLentiviralTagsmScarlet and popZExpressionBacterial and MammalianMutationPromoterUBC promoterAvailable sinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TRE3G-LbNOX-Flag
Plasmid#234980PurposeExpresses LbNOX in mouse cell linesDepositorInsertLbNOX
UseLentiviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TRE3G-mitoLbNOX-Flag
Plasmid#234981PurposeExpresses mitochondrial LbNOX in mouse cell linesDepositorInsertmito-LbNOX
UseLentiviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEF1-Pls3-sfGFP
Plasmid#205750PurposeExpresses sfGFP under IPTG inducible promoter Pls3 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. Medium/High strength.DepositorTypeEmpty backboneUseShuttle vector gram+ gram-TagsExpressionBacterialMutationPromoterPls3 (Phyperspank mutant, IPTG inducible)Available sinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEF1-Pls4-sfGFP
Plasmid#205751PurposeExpresses sfGFP under IPTG inducible promoter Pls4 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. High strength.DepositorTypeEmpty backboneUseShuttle vector gram+ gram-TagsExpressionBacterialMutationPromoterPls4 (Phyperspank mutant, IPTG inducible)Available sinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TetOne-Puro-LbNOX-Flag
Plasmid#234984PurposeExpresses LbNOX in human cell linesDepositorInsertLbNOX
UseLentiviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUC57-mitoLbNOX
Plasmid#74448PurposeThis plasmid contains human codon optimized sequence of mitoLbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.DepositorInsertmitoLbNOX
UseTagsFlag tag with linker and mitochondrial targeting …ExpressionMutationHuman codon optimizedPromoterAvailable sinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TetOne-Puro-mitoLbNOX-Flag
Plasmid#234985PurposeExpresses mitochondrial LbNOX in human cell linesDepositorInsertmito-LbNOX
UseLentiviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMM7
Plasmid#201213PurposeIntegrative GFP-based shuttle vector for genetic engineering of Parageobacillus thermoglucosidasius. Carries homologous flanks for deletion of key sporulation regulator spo0A.DepositorInsertssfGFP
Spo0A right flank
Spo0A left flank
UseSynthetic BiologyTagsExpressionBacterialMutationF11V, T13P, N39D, A179APromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGMβ1
Plasmid#216202PurposeChromosomal gene manipulation (gene insertion, conversion, deletion) of dairy used Lactobacillus bulgaricus, a difficult gene to manipulate, is now possible by conjugation using this pGMB1 plasmid.DepositorTypeEmpty backboneUseShuttle vector, conjugal plasmid, theta type rep…TagsExpressionBacterialMutationPromoterlac promoter in pGEM-Teasy, Promoters in pAMbeta1…Available sinceJan. 21, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTTCC
Plasmid#164264PurposeExpresses a thermostable Cas9 enzyme and gRNA in Thermus thermophilusDepositorInsertsCas9
gRNA scaffold
UseTagsExpressionBacterialMutationPromoterPcel and trcAvailable sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21a(+)-Bst LF-6xHis
Plasmid#159148Purposeexpresses His-tagged large fragment of Bst polymeraseDepositorInsertlarge fragment of Geobacillus stearothermophilus DNA polymerase I
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 1, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pThermoCas9_ctrl
Plasmid#100981PurposeExpresses ThermoCas9 and its sgRNA moduleDepositorInsertsCas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
ThermoCas9 single guide RNA expressing module
UseTagsNoneExpressionBacterialMutationPromoterB. coagulans DSM 1 pta promoter and B. smithii xy…Available sinceNov. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pThermoCas9i_ctrl
Plasmid#100982PurposeExpresses the catalytically inactive version of ThermoCas9 (Thermo(d)Cas9) and its sgRNA moduleDepositorInsertsCatalytically inactive variant of the Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
ThermoCas9 single guide RNA expressing module
UseTagsNoneExpressionBacterialMutationchanged aspartate 8 to alanine and histidine 582 …PromoterB. coagulans DSM 1 pta promoter and B. smithii xy…Available sinceNov. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGLU_81
Plasmid#225782PurposeRS421 library variant 13401DepositorInsert13401_[abr]
UseSynthetic BiologyTagsExpressionMutationN/APromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGLU_99
Plasmid#225812PurposeRS421 library variant 13401DepositorInsert13401_[Himar]
UseSynthetic BiologyTagsExpressionMutationN/APromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGLU_117
Plasmid#225797PurposeRS421 library variant 13401DepositorInsert13401_[Tn5]
UseSynthetic BiologyTagsExpressionMutationN/APromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL0_169 [pTHT15 ORI]
Plasmid#210688PurposeLevel 0 partDepositorInsertpTHT15 ORI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKAR2-Br512g3.1
Plasmid#171200PurposeExpresses Br512g3.1 polymerase in E.coliDepositorInsertEngineered Bst DNA Polymerase I Large Fragment
UseTags8x His tag; Villin Headpiece 47 amino acid (vHP47…ExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKAR2-Br512g3.2
Plasmid#171201PurposeExpresses Br512g3.2 polymerase in E.coliDepositorInsertEngineered Bst DNA Polymerase I Large Fragment
UseTags8x His tag; Villin Headpiece 47 amino acid (vHP47…ExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-FPPS
Plasmid#108952Purposeheterologous expression of FPPS of G. stearothermophilus in E. coliDepositorInsertFarnesyldiphosphat synthase (FPPS)
UseTagsHis6-TagExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
S7SZ03
Plasmid#163199PurposeInducible expression of UniProt identifier S7SZ03DepositorInsertS7SZ03
UseTags10xHis and T7ExpressionBacterialMutationPromoterT7Available sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCB591
Plasmid#141096PurposeE. coli-Lactobacilli shuttle vector for recombineering template cloningDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTec_mCherry
Plasmid#229145PurposePromoter strength evaluation of PtecDepositorInsertmCherry
UseTagsExpressionBacterialMutationNoPromoterPtecAvailable sinceJan. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSL4164 (pEffector_IscB,ωRNA)
Plasmid#210874PurposeExpresses GstIscB and native ωRNA from constitutive promotersDepositorInsertGstIscB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-AsCas12f1-v5.1+T1-empty
Plasmid#204977PurposeAsCas12f1-v5.1+T1-based Human cell genome editingDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationN70Q/K103R/A104R/S118A/D364RPromoterPCMV,U6Available sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSL4369 (pEffector_TnpB2,ωRNA)
Plasmid#210871PurposeExpresses GstTnpB2 and native ωRNA from constitutive promotersDepositorInsertGstTnpB2
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS-ermGFP
Plasmid#27172DepositorInserterythromycin ribosomal methylase promoter region fused with modified green fluorescent protein 5
UseTagsExpressionBacterialMutationN/APromoterAvailable sinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSL4672 (pEffector_TnpB3,ωRNA)
Plasmid#210872PurposeExpresses GstTnpB3 and native ωRNA from constitutive promotersDepositorInsertGstTnpB3
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSL4673 (pEffector_TnpB4,ωRNA)
Plasmid#210873PurposeExpresses GstTnpB4 and native ωRNA from constitutive promotersDepositorInsertGstTnpB4
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
M4KHX7
Plasmid#163210PurposeInducible expression of UniProt identifier M4KHX7DepositorInsertM4KHX7
UseTags10xHis and T7ExpressionBacterialMutationPromoterT7Available sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
B1HZY7
Plasmid#163229PurposeInducible expression of UniProt identifier B1HZY7DepositorInsertB1HZY7
UseTags10xHis and T7ExpressionBacterialMutationPromoterT7Available sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
I7IZ16
Plasmid#163243PurposeInducible expression of UniProt identifier I7IZ16DepositorInsertI7IZ16
UseTags10xHis and T7ExpressionBacterialMutationPromoterT7Available sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
G6F8W6
Plasmid#163204PurposeInducible expression of UniProt identifier G6F8W6DepositorInsertG6F8W6
UseTags10xHis and T7ExpressionBacterialMutationPromoterT7Available sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only