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Plasmid#64166Purposeharbours marker genes HIS3, LEU2, URA3 and MET17DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only
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pRN1120
Plasmid#101750PurposeMulti-copy (2 micron) yeast plasmid containing a functional NatMX marker cassette conferring resistance against nourseothricin. Recipient plasmid for sgRNA or crRNA expression cassettes.DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPM28 (eroGFP CEN/ARS URA3)
Plasmid#20131DepositorInserteroGFP (KAR2 Budding Yeast, jellyfish)
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
KBB460
Plasmid#185094PurposeNMA111-GFP wild type under endogenous promoterDepositorInsertNMA111
UseTagsGFPExpressionYeastMutationNMA111-GFPPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
bRA89
Plasmid#100950PurposeCas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. HPH markerDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
MK1283
Plasmid#71428PurposeThis E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 1535 and a TetR translation rate of 30709DepositorInsertMixed Feedback Loop version of the UBER system
UseTagsExpressionMutationT7 RBS: AACCGAGCCCAATATAGGACCTAGGGTGCCAAAAAA and …PromoterAvailable sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
bRA77
Plasmid#100953PurposeGAL1 driven Cas9. gRNA can be cloned into BplI sites.DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
bRA90
Plasmid#100951PurposeCas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. LEU2 markerDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDUP3
Plasmid#92174PurposeVector containing the two fragments comprising the 550 bp NEUT5L region with NAT1 marker. Integration by duplication.DepositorInsertNAT1
UseGenbank: u03440.1TagsExpressionYeastMutationPromoterAvailable sinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUNIV-EGFP
Plasmid#24706DepositorInsertEGFP (eGFP Aequoria victoria)
UseTagsExpressionBacterial, Mammalian, and Y…MutationPromoterAvailable sinceDec. 6, 2010AvailabilityAcademic Institutions and Nonprofits only -
bRA66
Plasmid#100952PurposeGAL1 driven Cas9. gRNA can be cloned into BplI sites.DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSCupSup35GFP
Plasmid#1087DepositorInsertSUP35 (SUP35 Budding Yeast)
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceAug. 9, 2005AvailabilityAcademic Institutions and Nonprofits only -
MK1274
Plasmid#71431PurposeThis E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 300 and a TetR translation rate of 35149.DepositorInsertMixed Feedback Loop version of the UBER system
UseTagsExpressionMutationT7 RBS:AACCGAGCCCAATATAGGACTCAGGGTGCCAAAAAA and T…PromoterAvailable sinceDec. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pABY-c46
Plasmid#201774PurposeExpresses mNeonGreen-tagged NbALFA under TEF1 promoter in yeastDepositorInsertNbALFA
UseTags40aa Linker, mNeonGreenExpressionYeastMutationPromoterTEF1Available sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pABY-c25
Plasmid#201769PurposeExpresses mNeonGreen-tagged NbALFA under CYC1 promoter in yeastDepositorInsertNbALFA
UseTags40aa Linker, mNeonGreenExpressionYeastMutationPromoterCYC1Available sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pABY-c15
Plasmid#201768PurposeExpresses mNeonGreen-tagged NbALFA under ADH1 promoter in yeastDepositorInsertNbALFA
UseTags40aa Linker, mNeonGreenExpressionYeastMutationPromoterADH1Available sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pACBB-eGFP
Plasmid#32551DepositorInserteGFP (eGFP )
UseSynthetic BiologyTagsHisExpressionMutationPromoterConsitutive lacAvailable sinceNov. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCUP1pNuiHA kanMX CEN
Plasmid#131168Purposefor ScCUP1 promoter regulated N-terminal fused Nui-HA prey expression, centromeric ARS plasmid, kanMX marker confers resistance to geneticin in S. cerevisiaeDepositorTypeEmpty backboneUseTagsNui-HAExpressionYeastMutationPromoterAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
Spt2-GFP
Plasmid#115572PurposeExpresses yeast Spt2-GFP fusion proteinDepositorInsertSPT2
UseTagsGFPExpressionBacterial and YeastMutationPromoterAvailable sinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LDB107
Plasmid#185423PurposeNUP1-HA CEN TRP1 AmpR. Yeast shuttle plasmid for expression of hemagluttinin tagged NUP1 nucleoporinDepositorInsertNUP1
UseTagsHAExpressionYeastMutationPromoterAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
p817-mgammaFcry-EGFP
Plasmid#23156DepositorInsertmouse gammaF crystallin promoter:EGFP (eGFP Mouse, mouse gammaF promoter:EGFP)
UseMulticloning site flanked by i-scei sitesTagsExpressionMutationmouse gammaF crystallin promoter (-225 - +73) pre…PromoterAvailable sinceApril 13, 2010AvailabilityAcademic Institutions and Nonprofits only -
KBB285
Plasmid#185076PurposeRFA3-GFP fusionDepositorInsertRFA3
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB284
Plasmid#185075PurposeRFA2-GFP fusionDepositorInsertRFA2
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTEF-Sup35C
Plasmid#1202DepositorInsertSup35 C-terminus (SUP35 Budding Yeast)
UseTagsExpressionYeastMutationSup35 C-terminus ~254aa-endPromoterTEFAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pYC44
Plasmid#63903PurposeInitial Integrative VectorDepositorTypeEmpty backboneUseTagsFRT::3'UTRcta::NAT::FRTExpressionYeastMutationPromoterAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pR184
Plasmid#47952PurposertTA positive feedback loop with three tet operators in the GAL1 promoterDepositorInsertrtTA
UseTet inducible expressionTagsExpressionYeastMutationPromoterGal1Available sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
GFP-CDC12(WT)
Plasmid#1168DepositorInsertCDC12 (CDC12 Budding Yeast)
UseTagsGFPExpressionYeastMutationAdded N-terminal GFP to CDC12 in plasmid CDC12(WT…PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCH001
Plasmid#47953PurposetetO-GFP reporter casetteDepositorInsertGFP
UseTet inducible expressionTagsExpressionYeastMutationPromoterT3Available sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCH043
Plasmid#47949PurposertTA under the control of GAL1 promoterDepositorInsertrtTA
UseTet inducible expressionTagsExpressionYeastMutationPromoterGal1Available sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCH044
Plasmid#47950PurposertTA under the control of GAL2 promoterDepositorInsertrtTA
UseTet inducible expressionTagsExpressionYeastMutationPromoterGal2Available sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
piHGpd/p82
Plasmid#1105DepositorInsertHSP82 (HSP82 Budding Yeast)
UseTagsExpressionYeastMutationwild-type HSP82 regulated by the strong constitut…PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
piHGpd/G170D
Plasmid#1113DepositorInsertHSP82 (HSP82 Budding Yeast)
UseTagsExpressionYeastMutationHSP82 with glycine 170 changed to aspartic acid; …PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
sup35-C653R
Plasmid#1198DepositorInsertSup35p (SUP35 Budding Yeast)
UseTagsExpressionYeastMutationchanged cys 653 to argPromoterAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pJLI-Sup35?M
Plasmid#1225DepositorInsertSUP35 (SUP35 Budding Yeast)
UseYeast integrative plasmidTagsExpressionMutationdeletion of aa 124-253 of SUP35PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
sup35-R320I
Plasmid#1199DepositorInsertSup35p (SUP35 Budding Yeast)
UseTagsExpressionYeastMutationchanged arg 320 to isoleucinePromoterAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
piHGpd/T101I
Plasmid#1112DepositorInsertHSP82 (HSP82 Budding Yeast)
UseTagsExpressionYeastMutationHSP82 with threonine 101 changed to isoleucine; r…PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
PAF23
Plasmid#223171PurposeExpresses yeGFP under control of CTR1 promoter and terminatorDepositorInsertgreen fluorescent protein
UseTagsExpressionYeastMutationPromoterSaccharomyces cerevisiae CTR1Available sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
PAF24
Plasmid#223172PurposeExpresses VC83_00191-yeGFP under control of CTR1 promoter and terminatorDepositorInsertCopper Transporter
UseTagsStreptagII and yeGFPExpressionYeastMutationPromotersaccharomyces cerevisiae CTR1Available sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
KBB520
Plasmid#185102Purpose1st 200 amino acids (N-term + transmembrane domain) of Pom152 fused to HA and GFPDepositorInsertPOM152
UseTagsGFPExpressionYeastMutationPOM152(1-200)-GFPPromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB521
Plasmid#185103Purpose1st 960 amino acids (C-terminal truncation) of Pom152 fused with HA and GFPDepositorInsertPOM152
UseTagsGFPExpressionYeastMutationPOM152(1-960)-HA-GFPPromoterAvailable sinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB329
Plasmid#185077PurposeNUP82 expression in yeastDepositorInsertNUP82
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB280
Plasmid#185073PurposeLarger fusion of Nma111 nuclear localization signal to GFPDepositorInsertNMA111
UseTagsGFPExpressionYeastMutationNMA111 aa 1-83PromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB382
Plasmid#185085PurposeHA-tagged POM152 with glycosylation sites mutatedDepositorInsertPOM152
UseTagsHAExpressionYeastMutationPom152 N280A, N398A, N???A, N???A = Pom152DglycPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB282
Plasmid#185074PurposeSmaller fusion of Nma111 nuclear localization signal to GFPDepositorInsertNMA111
UseTagsGFPExpressionYeastMutationNMA111 aa 1-35PromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB465
Plasmid#185098PurposeNMA111-GFP with NLS1 deleted under endogenous promoterDepositorInsertNMA111
UseTagsGFPExpressionYeastMutationNMA111DNLS1-GFPPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB466
Plasmid#185099PurposeNMA111-GFP with NLS1 and NLS2 deleted under endogenous promoterDepositorInsertNMA111
UseTagsGFPExpressionYeastMutationNMA111DNLS1DNLS2-GFPPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB467
Plasmid#185100PurposeNMA111-GFP with NLS2 deleted under endogenous promoterDepositorInsertNMA111
UseTagsGFPExpressionYeastMutationNMA111DNLS2-GFPPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only