We narrowed to 1,593 results for: PTS

1. KBB439

   Plasmid

   #185093

   Purpose

   NMA111-GFP wild type under control of GAL1 promoter (complements nma111 deletion)

   Depositor

   Ken Belanger

   Article

   Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

   Insert

   NMA111
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   GAL1::NMA111-GFP

   Promoter

   GAL1

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. KBB280

   Plasmid

   #185073

   Purpose

   Larger fusion of Nma111 nuclear localization signal to GFP

   Depositor

   Ken Belanger

   Article

   Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

   Insert

   NMA111
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   NMA111 aa 1-83

   Promoter

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. KBB282

   Plasmid

   #185074

   Purpose

   Smaller fusion of Nma111 nuclear localization signal to GFP

   Depositor

   Ken Belanger

   Article

   Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

   Insert

   NMA111
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   NMA111 aa 1-35

   Promoter

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. KBB465

   Plasmid

   #185098

   Purpose

   NMA111-GFP with NLS1 deleted under endogenous promoter

   Depositor

   Ken Belanger

   Article

   Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

   Insert

   NMA111
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   NMA111DNLS1-GFP

   Promoter

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. KBB462

   Plasmid

   #185095

   Purpose

   NMA111-GFP with NLS1 and NLS2 mutated under control of GAL1 promoter

   Depositor

   Ken Belanger

   Article

   Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

   Insert

   NMA111
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   GAL1::nma111Dnls1Dnls2-GFP

   Promoter

   GAL1

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. KBB466

   Plasmid

   #185099

   Purpose

   NMA111-GFP with NLS1 and NLS2 deleted under endogenous promoter

   Depositor

   Ken Belanger

   Article

   Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

   Insert

   NMA111
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   NMA111DNLS1DNLS2-GFP

   Promoter

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. KBB467

   Plasmid

   #185100

   Purpose

   NMA111-GFP with NLS2 deleted under endogenous promoter

   Depositor

   Ken Belanger

   Article

   Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

   Insert

   NMA111
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   NMA111DNLS2-GFP

   Promoter

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. OfCasp3b

   Plasmid

   #166054

   Purpose

   a caspase of Orbicella faveolata, which has higher sequence similarity with human caspase-3 but function similar to caspase-6

   Depositor

   Clay Clark

   Article

   Shrestha et al J Biol Chem. 2020 Oct 23;295(43):14578-1

   Insert

   Orbicella faveolata caspase-3b
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 lac promoter

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PaCasp3

   Plasmid

   #166052

   Purpose

   a caspase of Porites astreoides, which has higher sequence similarity with human caspase-3 but function similar to caspase-6

   Depositor

   Clay Clark

   Article

   Shrestha et al J Biol Chem. 2020 Oct 23;295(43):14578-1

   Insert

   Porites astreiodes caspase-3
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 lac promoter

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Lf-MetQ

    Plasmid

    #78305

    Purpose

    Methionine Binding Protein (MetQ) with C-Terminal 6x Histidine Tag

    Depositor

    Douglas Rees

    Article

    Nguyen et al Biol Chem. 2015 Sep;396(9-10):1127-34. d

    Insert

    Methionine Binding Protein (metQ )
    

    Use

    Tags

    6x Histidine Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSEW-Venus-Sec16A-deltaN

    Plasmid

    #66605

    Purpose

    Lentiviral vector for express truncated form of Sec16A as a fusion with Venus FP

    Depositor

    David Stephens

    Article

    Hughes et al J Cell Sci. 2010 Dec 1;123(Pt 23):4032-8

    Insert

    Sec16A (SEC16A Human)
    

    Use

    Tags

    Venus (EYFP derivative)

    Expression

    Mammalian

    Mutation

    encodes amino acids 1107-1217 of human Sec16A

    Promoter

    Available since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

12. EGFP-alphasynuclein-A53T

    Plasmid

    #40823

    Depositor

    David Rubinsztein

    Article

    Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

    Insert

    SNCA (SNCA Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    A53T (aggregates more rapidly than the wild-type …

    Promoter

    CMV

    Available since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEGFP-N2-COL1A1

    Plasmid

    #66602

    Purpose

    Expresses procollagen 1 alpha 1 as a GFP fusion

    Depositor

    David Stephens

    Article

    Stephens et al J Cell Sci. 2002 Mar 15;115(Pt 6):1149-6

    Insert

    COL1A1 (COL1A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. HA GSK3 beta S9A pcDNA3

    Plasmid

    #14754

    Purpose

    Mammalian expression of human GSK3 beta constitutively active mutant with HA tag

    Depositor

    Jim Woodgett

    Article

    Stambolic et al Biochem J. 1994 Nov 1. 303 ( Pt 3)():701

    Insert

    GSK3 beta S9A (GSK3B Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S9A. Constitutively active mutant.

    Promoter

    Available since

    April 12, 2007

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pIRES2-EGFP Mer

    Plasmid

    #14998

    Depositor

    Raymond Birge

    Article

    Wu et al J Cell Sci. 2005 Feb 1. 118(Pt 3):539-53

    Insert

    Mer (Mertk Mouse)
    

    Use

    Tags

    IRES EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEYFP-Sec24C

    Plasmid

    #66608

    Purpose

    YFP fusion of Sec24C

    Depositor

    David Stephens

    Article

    Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

    Insert

    Sec24C (SEC24C Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-Flag Rac2

    Plasmid

    #12192

    Depositor

    Gary Johnson

    Article

    Abell et al J Cell Sci. 2004 Jan 15. 117(Pt 2):243-5

    Insert

    Rac2 (RAC2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGL4.10/RSV_SF3B3-miniXon_Luciferase

    Plasmid

    #174660

    Purpose

    Plasmid containing the SF3B3-miniXon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-miniXon by LMI070.

    Depositor

    Beverly L. Davidson

    Article

    Monteys et al Nature. 2021 Jul 28. pii: 10.1038/s41586

    Insert

    miniXon cassette
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Kozak ATG initiation sequence in middle exon

    Promoter

    RSV

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEGFP-p150Glued

    Plasmid

    #36154

    Depositor

    David Stephens

    Article

    Watson et al J Cell Sci. 2006 Jul 1;119(Pt 13):2758-6

    Insert

    p150Glued (DCTN1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    see documentation

    Promoter

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL4.10/RSV_SF3B3-Xon_Luciferase

    Plasmid

    #174659

    Purpose

    Plasmid containing the SF3B3-Xon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-xon by LMI070.

    Depositor

    Beverly L. Davidson

    Article

    Monteys et al Nature. 2021 Jul 28. pii: 10.1038/s41586

    Insert

    Xon cassette
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Kozak ATG initiation sequence in middle exon

    Promoter

    RSV

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. FU- dio PSD95-mCherry- W

    Plasmid

    #73919

    Purpose

    Expresses PSD-95 tagged with mCherry. Expression is Cre dependent. Plasmid can also be used to make lentivirus.

    Depositor

    Elly Nedivi

    Article

    Villa et al Neuron. 2016 Feb 3. pii: S0896-6273(16)0

    Insert

    PSD-95-mCherry
    

    Use

    Lentiviral

    Tags

    mCherry and myc

    Expression

    Mammalian

    Mutation

    Promoter

    Ubi-C

    Available since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. FU- dio Teal-gephyrin - W

    Plasmid

    #73918

    Purpose

    To express the inhibitory scaffold gephyrin tagged to teal fluorescent protein. Expression is Cre dependant. Plasmid can also be used to make lentivirus.

    Depositor

    Elly Nedivi

    Article

    Villa et al Neuron. 2016 Feb 3. pii: S0896-6273(16)0

    Insert

    Teal-gephyrin
    

    Use

    Lentiviral

    Tags

    Teal

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEGFP-mAV

    Plasmid

    #129472

    Purpose

    Mammalian expression of full-length mouse archvillin (Supervillin, isoform 2)

    Depositor

    Elizabeth Luna

    Article

    Oh et al J Cell Sci. 2003 Jun 1. 116(Pt 11):2261-

    Insert

    Supervillin, isoform 2 (Svil Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLC133

    Plasmid

    #198639

    Purpose

    Expression plasmid for dCas9 in E. coli used in Chip-seq experiments. dCas9 is tagged with a C-terminal 3x FLAG tag. A guide RNA can be cloned using BsaI restriction sites.

    Depositor

    David Bikard

    Article

    Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.

    Insert

    dCas9
    

    Use

    Tags

    3x-FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Human SERCA3 (pMT2)

    Plasmid

    #75189

    Purpose

    Mammalian expression of ATP2A3

    Depositor

    Jonathan Lytton, David MacLennan

    Article

    Poch et al Am J Physiol. 1998 Dec;275(6 Pt 1):C1449

    Insert

    ATP2A3 (ATP2A3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    adenovirus major late promoter

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pIRES2-EGFP Rac2

    Plasmid

    #12193

    Depositor

    Gary Johnson

    Article

    Abell et al J Cell Sci. 2004 Jan 15. 117(Pt 2):243-5

    Insert

    Rac2 (RAC2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEYFP-Sec24D

    Plasmid

    #66614

    Purpose

    YFP fusion of Sec24D

    Depositor

    David Stephens

    Article

    Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

    Insert

    Sec24D (SEC24D Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

28. GFP-Myo 1c

    Plasmid

    #134832

    Purpose

    Expression of rat Myo 1c (Myr 2) in mammalian cells. N-terminal GFP-tag.

    Depositor

    Martin Bähler

    Article

    Ruppert et al J Cell Sci. 1995 Dec;108 ( Pt 12):3775-8

    Insert

    Myo 1c (rat) with 3' UTR (Myo1c Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pPBJ pFOS-KTR-Gal4-PEST-tub3’ pCMV-TagBFP

    Plasmid

    #176531

    Purpose

    PiggyBAC vector expressing a FOS promoter driven "KGV" synthetic transcription factor and a CMV promoter driven BFP marker.

    Depositor

    Jared Toettcher

    Article

    Ravindran et al Cell Syst. 2022 Feb 16;13(2):131-142.e13

    Inserts

    KGV transcription factor
    TagBFP
    

    Use

    Piggybac transposase integration vector

    Tags

    ErkKTR, Gal4 DNA binding domain, PEST destabiliza…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Histone H2B targeted Aurora B FRET sensor Kif2 substrate

    Plasmid

    #45216

    Depositor

    Michael Lampson

    Article

    Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

    Insert

    Histone H2B-targeted Aurora B FRET sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    May 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Rix-PGK-Tom-W

    Plasmid

    #25813

    Depositor

    Jozsef Kiss, Patrick Salmon

    Article

    Dayer et al Brain. 2007 Nov . 130(Pt 11):2962-76.

    Insert

    Rix-PGK-Tom-W
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    tdTomato from Tsien lab

    Promoter

    Available since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. untargeted Plk1 FRET sensor Myt1 substrate

    Plasmid

    #45217

    Depositor

    Michael Lampson

    Article

    Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

    Insert

    untargeted Plk1 FRET sensor Myt1 substrate
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    t7

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. CENP-B targeted Aurora B FRET sensor Kif2 substrate

    Plasmid

    #45214

    Depositor

    Michael Lampson

    Article

    Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

    Insert

    CENP-B targeted Aurora B FRET sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    t7

    Available since

    May 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSin wPGK-Cre

    Plasmid

    #101242

    Purpose

    low-efficiency Cre used to turn on dio YFP/ PSD95-mCherry/ Teal-gephyrin expression

    Depositor

    Elly Nedivi

    Article

    Villa et al Neuron. 2016 Feb 3. pii: S0896-6273(16)0

    Insert

    Cre recombinase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    see attached file

    Promoter

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA5 FRT TO myc hSeparase

    Plasmid

    #59820

    Purpose

    Allows the integration of myc hSeparase in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Holland et al J Cell Sci. 2006 Aug 15;119(Pt 16):3325-

    Insert

    Separase (ESPL1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCAG-SRGAP1FBAR(516a.a.)-EGFP

    Plasmid

    #168505

    Purpose

    Expression of F-BAR domain (aa1-516) of SRGAP1 with a C-terminal EGFP fusion.

    Depositor

    Franck Polleux

    Article

    Coutinho-Budd et al J Cell Sci. 2012 Jul 15;125(Pt 14):3390-

    Insert

    SRGAP1-FBAR (SRGAP1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa 1-516

    Promoter

    CAG

    Available since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCI-ING1b

    Plasmid

    #79052

    Purpose

    expression of p33ING1b in mammalian cells

    Depositor

    Karl Riabowol

    Article

    Scott et al J Cell Sci. 2001 Oct;114(Pt 19):3455-62.

    Insert

    ING1 (p33 ING1Ab isoform) (ING1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

38. pEF1α-mouse-retroCHMP3-HA

    Plasmid

    #154178

    Purpose

    expresses mouse retroCHMP3 in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

    Insert

    retroCHMP3
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA5 FRT TO myc Separase C2029A

    Plasmid

    #59822

    Purpose

    Allows the integration of myc Separase C2029A in the genome and Tet-inducible expression

    Depositor

    Stephen Taylor

    Article

    Holland et al J Cell Sci. 2006 Aug 15;119(Pt 16):3325-

    Insert

    Separase (ESPL1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    C2029A

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. LDB204

    Plasmid

    #185426

    Purpose

    Bacterial expression of FG-repeat domain of NUP1 nucleoporin as GST-Nup1-Rep fusion

    Depositor

    Laura Davis, Ken Belanger

    Article

    Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

    Insert

    NUP1
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Nup1 FXFG-repeats domain expressed

    Promoter

    Available since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pECFP-N2-COL1A1

    Plasmid

    #66603

    Purpose

    Expresses procollagen 1 alpha 1 as a CFP fusion

    Depositor

    David Stephens

    Article

    Stephens et al J Cell Sci. 2002 Mar 15;115(Pt 6):1149-6

    Insert

    COL1A1 (COL1A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

42. pCDH-AtAFB2-mTagBFP2

    Plasmid

    #179889

    Purpose

    Expresses AtAFB2 (auxin-inducible degron system) fused to mTagBFP2 (lentiviral plasmid)

    Depositor

    Patrick Caswell

    Article

    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

    Insert

    AtAFB2 (AFB2 Synthetic, Mustard Weed)
    

    Use

    Lentiviral

    Tags

    mTagBFP2

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF1

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCDNA CrkII Y221F

    Plasmid

    #50732

    Purpose

    mammalian expression of CrkII Y221F mutant

    Depositor

    Kenneth Yamada

    Article

    Takino et al J Cell Sci. 2003 Aug 1;116(Pt 15):3145-5

    Insert

    CrkII Y221F (CRK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Y221F

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

44. pCAG-SRGAP3FBAR-EGFP

    Plasmid

    #168506

    Purpose

    Expression of F-BAR domain (aa 1-492) of SRGAP3 with a C-terminal EGFP fusion

    Depositor

    Franck Polleux

    Article

    Coutinho-Budd et al J Cell Sci. 2012 Jul 15;125(Pt 14):3390-

    Insert

    SRGAP3-FBAR (SRGAP3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa 1-492

    Promoter

    CAG

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. cmamxA1-GFP

    Plasmid

    #107195

    Purpose

    Expresses porcine annexin A1-GFP fusion protein for live cell imaging, all type II Ca2+ binding sites deleted

    Depositor

    Ursula Rescher, Volker Gerke

    Article

    Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

    Insert

    cmannexin A1
    

    Use

    Tags

    Green Fluorescent Protein

    Expression

    Mammalian

    Mutation

    exchanges Asp170Ala, Glu255Ala, Glu330Ala, all ty…

    Promoter

    CMV

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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46. EGFP-bSV-831-1281

    Plasmid

    #40962

    Depositor

    Elizabeth Luna

    Article

    Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

    Insert

    supervillin (SVIL Bovine)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-830 and 1282-1792

    Promoter

    Available since

    Dec. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

47. KBB394

    Plasmid

    #185086

    Purpose

    NMA111 with both nuclear localization signals mutated to alanines fused to GFP (mutagenesis of KBB280)

    Depositor

    Ken Belanger

    Article

    Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

    Insert

    NMA111
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Nma111 KKR 9-11 AAA; KRK 28-30 AAA

    Promoter

    Available since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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48. pJET-10-mCherry-R11b

    Plasmid

    #179891

    Purpose

    Donor with homologous arms for Rab11b to knock in mCherry

    Depositor

    Patrick Caswell

    Article

    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

    Insert

    RAB11B, member RAS oncogene family (RAB11B Human)
    

    Use

    CRISPR; Donor for homologous based knock in

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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49. OfCasp3a

    Plasmid

    #166053

    Purpose

    a caspase of Orbicella faveolata, which has higher sequence similarity with human caspase-3 but has CARD domain and function as an initiator caspase

    Depositor

    Clay Clark

    Article

    Shrestha et al J Biol Chem. 2020 Oct 23;295(43):14578-1

    Insert

    Orbicella faveolata caspase-3a
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 lac promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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50. PaCasp7a

    Plasmid

    #166051

    Purpose

    a caspase of Porites astreoides, which has higher sequence similarity with human caspase-7 but has a CARD domain and function as an initiator caspase

    Depositor

    Clay Clark

    Article

    Shrestha et al J Biol Chem. 2020 Oct 23;295(43):14578-1

    Insert

    Porites astreiodes caspase-7a
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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