We narrowed to 674 results for: RPL

1. MYH11-NanoLuc-2A-tdTomato

   Plasmid

   #132569

   Purpose

   Donor vector to knockin NanoLuc and tdTomado (separated by 2A) into MYH11 allele

   Depositor

   James Thomson

   Article

   Zhang et al Stem Cell Reports. 2019 Jun 11;12(6):126

   Insert

   Human MYH11 knockin homology arms (MYH11 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pALS2-sfGFP 150TAG

   Plasmid

   #197574

   Purpose

   Fluorescence selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-150TAG and M. alvus Pyl-tRNA(6). p15a origin of replication.

   Depositor

   Ryan Mehl

   Article

   Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

   Inserts

   sfGFP 150TAG
   M. alvus Pyl-tRNA (6)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   N150TAG

   Promoter

   araC and lpp

   Available since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAcBac1-Mb-sfGFP WT

   Plasmid

   #197569

   Purpose

   Expression of sfGFP WT with Methanosarcina barkeri (Mb) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

   Depositor

   Ryan Mehl

   Article

   Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

   Inserts

   sfGFP WT
   Pyl-tRNA (4 copies)
   

   Use

   Tags

   V5-His6

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6/H1

   Available since

   April 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAcBac1-Ma-sfGFP 150TAG

   Plasmid

   #197568

   Purpose

   Expression of sfGFP-150TAG with Methanomethylophilus alvus (Ma) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

   Depositor

   Ryan Mehl

   Article

   Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

   Inserts

   sfGFP 150TAG
   M. alvus Pyl-tRNA (6) (4x copies)
   

   Use

   Tags

   V5-His6

   Expression

   Mammalian

   Mutation

   N150TAG

   Promoter

   CMV and U6/H1

   Available since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTRE3G-EGFPC-uL10-(Neo)R

   Plasmid

   #158069

   Purpose

   eGFP and uL10 ribosomal protein under the TRE3G promoter, Neo resistance

   Depositor

   Adolfo Rivero-Muller

   Article

   Michalec-Wawiorka et al Int J Mol Sci. 2021 May 14;22(10). pii:

   Insert

   RPLP0 (RPLP0 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   Nov. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGEX Grap2-SH2

   Plasmid

   #46435

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Grap2 (GRAP2 Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 52-159

   Promoter

   Tac

   Available since

   July 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDule2-3-nitroTyrosine (A7)

   Plasmid

   #174079

   Purpose

   Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.

   Depositor

   Ryan Mehl

   Article

   Beyer et al J Mol Biol. 2020 Jul 24;432(16):4690-470

   Insert

   3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y32H H70T D158H I159A L162R

   Promoter

   GlnRS (constitutive)

   Available since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Ma-Barnase

   Plasmid

   #197572

   Purpose

   Negative selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses 2xTAG-codon interrupted barnase gene and M. alvus Pyl-tRNA(6). p15a origin of replication.

   Depositor

   Ryan Mehl

   Article

   Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

   Inserts

   Barnase - 2xTAG
   M. alvus Pyl-tRNA (6)
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   3TAG and 45TAG

   Promoter

   araC and lpp

   Available since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Ma-pREP

   Plasmid

   #197571

   Purpose

   Positive selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses TAG-codon interrupted chloramphenicol resistance cassette and M. alvus Pyl-tRNA(6). p15a ori

   Depositor

   Ryan Mehl

   Article

   Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

   Inserts

   Chloramphenicol resistance gene - 111TAG
   T7 polymerase -TAG
   GFPuv
   M. alvus Pyl-tRNA(6)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   111TAG and 8TAG/112TAG

   Promoter

   AraC, T7, cat promoter (constitutive), and lpp (c…

   Available since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pERM2-nhpSer

    Plasmid

    #201922

    Purpose

    Enables translational incorporation of non-hydrolyzable phosphoserine into proteins at TAG codons in E coli. Do not use for phosphoserine incorporation.

    Depositor

    Ryan Mehl, Richard Cooley

    Article

    Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.

    Inserts

    SepRS(2)
    Sep-tRNA v2.0
    SerB
    EF-Sep
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    E412P, E414F, T417K, P495M, I496W, F529S and H67R…

    Promoter

    GlnS (constitutive), OXB20 (constitutive), lpp (c…

    Available since

    June 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAcBac1-Mb-sfGFP 150TAG

    Plasmid

    #197570

    Purpose

    Expression of sfGFP 150TAG with Methanosarcina barkeri (Mb) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    sfGFP 150TAG
    Pyl-tRNA (4 copies)
    

    Use

    Tags

    V5-His6

    Expression

    Mammalian

    Mutation

    N150TAG

    Promoter

    CMV and U6/H1

    Available since

    April 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDule-3-nitroTyrosine (A7)

    Plasmid

    #174078

    Purpose

    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Beyer et al J Mol Biol. 2020 Jul 24;432(16):4690-470

    Insert

    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y32H H70T D158H I159A L162R

    Promoter

    GlnRS (constitutive)

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMS-dsRed2-WT LMNA-GFP minigene

    Plasmid

    #128230

    Purpose

    Lamin A splicing reporter

    Depositor

    Tom Misteli

    Article

    Shilo et al Nucleic Acids Res. 2019 Apr 22. pii: 547

    Insert

    lamin A (LMNA Human)
    

    Use

    Tags

    GFP and SV40-driven dsRed2 to identify transfecte…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAcBac1-Ma-Acridone-RS1 (A7)

    Plasmid

    #197566

    Purpose

    Expression of Methanomethylophilus alvus (Ma) Acridone RS1 (A7) tRNA synthetase/Pyl-tRNA pair for the encoding of Acridone at TAG codons in HEK293 cells

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    M. alvus PylRS - Acd RS1 (A7)
    M. alvus Pyl-tRNA (6) (4x copies)
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    N166A, V168G, W239C

    Promoter

    CMV and U6/H1

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMS-dsRed2-C1824U LMNA-GFP minigene

    Plasmid

    #128231

    Purpose

    C1824U mutation Lamin A splicing reporter

    Depositor

    Tom Misteli

    Article

    Shilo et al Nucleic Acids Res. 2019 Apr 22. pii: 547

    Insert

    lamin A (LMNA Human)
    

    Use

    Tags

    GFP and SV40-driven dsRed2 to identify transfecte…

    Expression

    Mammalian

    Mutation

    C1824U

    Promoter

    CMV

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1(+)-C-6His-mEsrra

    Plasmid

    #173152

    Purpose

    mouse estrogen-related receptor alpha (Esrra) expression plasmid in mammalian cells

    Depositor

    Brijesh Kumar Singh

    Article

    Tripathi et al bioRxiv 2021.11.16.468891

    Insert

    ORF clone Estrogen-related receptor alpha (Esrra) (Esrra Mouse)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Wild type

    Promoter

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GFP Cav2.2 human HAgHA -plus exon 18a - LONG-Cterm in pcDNA3

    Plasmid

    #198451

    Purpose

    N-terminal GFP tagged, exofacial HAgHA tag in Domain II, contains exon 18a- Long form of alternatively spliced C-teminus, in pcDNA3 vector

    Depositor

    Annette Dolphin

    Article

    Dahimene et al Function (Oxf). 2023 Oct 19;5(1):zqad060

    Insert

    Cav2.2 (CACNA1B Human)
    

    Use

    Tags

    N-terminal GFP, internal double HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GFP Cav2.2 human HAgHA -minus exon 18a -LONG-Cterm in pcDNA3

    Plasmid

    #198914

    Purpose

    N-terminal GFP tagged, exofacial HAgHA tag in Domain II, does not contain exon 18a - Long form of alternatively spliced C-teminus, in pcDNA3 vector

    Depositor

    Annette Dolphin

    Article

    Dahimene et al Function (Oxf). 2023 Oct 19;5(1):zqad060

    Insert

    Cav2.2 (CACNA1B Human)
    

    Use

    Tags

    N-terminal GFP, internal double HA

    Expression

    Mammalian

    Mutation

    exon 18a deleted, exon 19 starts 3 nucleotides ea…

    Promoter

    CMV

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GFP Cav2.2 human HAgHA-plus exon 18a - Short-Cterm - Arginine -in pcDNA3

    Plasmid

    #198915

    Purpose

    N-terminal GFP tagged, exofacial HAgHA tag in Domain II, contains exon 18a, Short form of alternatively spliced C-teminus, SNP rs2278973 variant Arginine, in pcDNA3 vector

    Depositor

    Annette Dolphin

    Article

    Dahimene et al Function (Oxf). 2023 Oct 19;5(1):zqad060

    Insert

    Cav2.2 (CACNA1B Human)
    

    Use

    Tags

    N-terminal GFP, internal double HA

    Expression

    Mammalian

    Mutation

    snp rs2278973 Arginine

    Promoter

    CMV

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. P3-Lenti-dCas9-Tet1-GFP

    Plasmid

    #190729

    Purpose

    Lentiviral construct to express spdCas9-Tet1-GFP.

    Depositor

    Maja Jagodic

    Article

    Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:

    Insert

    dCas9-tet1
    

    Use

    CRISPR

    Tags

    SV40 NLS and T2A-EGFP

    Expression

    Mammalian

    Mutation

    Changed Pro434 codon from CCG to CCC and Gly468 f…

    Promoter

    UbC

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. GFP Cav2.2 human -HAgHA-minus exon 18a -Short-Cterm - Arg -in pcDNA3

    Plasmid

    #198916

    Purpose

    N-terminal GFP tagged, exofacial HAgHA tag in Domain II, does not contain exon 18a, Short form of alternatively spliced C-teminus, SNP rs2278973 variant Arginine, in pcDNA3 vector

    Depositor

    Annette Dolphin

    Article

    Dahimene et al Function (Oxf). 2023 Oct 19;5(1):zqad060

    Insert

    Cav2.2 (CACNA1B Human)
    

    Use

    Tags

    N-terminal GFP, internal double HA

    Expression

    Mammalian

    Mutation

    exon 18a deleted, exon 19 starts 3 nucleotides ea…

    Promoter

    CMV

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. P3-dCas9-Tet1-GFP-Puro

    Plasmid

    #190728

    Purpose

    Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)

    Depositor

    Maja Jagodic

    Article

    Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:

    Insert

    dCas9-tet1
    

    Use

    CRISPR

    Tags

    3XFLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Changed Pro434 codon from CCG to CCC and Gly468 f…

    Promoter

    pCAG

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMO14

    Plasmid

    #235736

    Purpose

    Expression of human VPS34 complex II in mammalian cells

    Depositor

    Roger Williams

    Article

    Mercer et al EMBO J. 2021 Jul 15;40(14):e105985. doi:

    Inserts

    ATG14L (ATG14 Human)
    UVRAG (UVRAG Human)
    EGFP
    BECN1 (BECN1 Human)
    PIK3C3 (PIK3C3 Human)
    PIK3R4 (PIK3R4 Human)
    

    Use

    Tags

    2xStrep-tagII-2xTEV-ZZ and ZZ-3xTEV

    Expression

    Mammalian

    Mutation

    N256S

    Promoter

    CMV

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pILGFP1DFB

    Plasmid

    #185871

    Purpose

    A parental plasmid for in vivo gene amplification at RPL25 locus

    Depositor

    Claudia Vickers

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pILGFP3AA5

    Plasmid

    #185875

    Purpose

    A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~47 copy)

    Depositor

    Claudia Vickers

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pILGFP3AG4

    Plasmid

    #185874

    Purpose

    A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~12 copy)

    Depositor

    Claudia Vickers

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pILGFP3AE4

    Plasmid

    #185873

    Purpose

    A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~11 copy)

    Depositor

    Claudia Vickers

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pILGFP3A5C

    Plasmid

    #185872

    Purpose

    A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (1 copy reference)

    Depositor

    Claudia Vickers

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pILGFP3AG4ARSd

    Plasmid

    #185876

    Purpose

    A plasmid without an ARS sequence for in vivo gene amplification (HamAmp) at RPL25 locus (~12 copy)

    Depositor

    Claudia Vickers

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pILAC3

    Plasmid

    #185890

    Purpose

    In vivo gene amplification (HamAmp) of lycopene synthetic module at RPL25 locus

    Depositor

    Claudia Vickers

    Insert

    see comments
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    ERG20 F96C; Xd.CRtYB E83K

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. pIT6EG7ml

    Plasmid

    #185886

    Purpose

    In vivo gene amplification (HamAmp) of limonene synthetic module at RPL25 locus (14 copy).

    Depositor

    Claudia Vickers

    Insert

    see comments
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    ERG20 F96W N127W

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pIT6EG7mlh

    Plasmid

    #185887

    Purpose

    In vivo gene amplification (HamAmp) of limonene synthetic module at RPL25 locus (23 copy).

    Depositor

    Claudia Vickers

    Insert

    see comments
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    ERG20 F96W N127W

    Promoter

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pILAC2

    Plasmid

    #185889

    Purpose

    In vivo gene amplification (HamAmp) of lycopene synthetic module at RPL25 locus

    Depositor

    Claudia Vickers

    Insert

    see comments
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    ERG20 F96C; Xd.CRtYB E83K

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pIT6EG7m

    Plasmid

    #185885

    Purpose

    A backbone plasmid for in vivo gene amplification (HamAmp) of a monoterpene synthetic module (+ GPP synthase without a monoterpene synthase) at RPL25 locus (14 copy)

    Depositor

    Claudia Vickers

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. mEmerald-UbiquitinC-C-18

    Plasmid

    #54294

    Purpose

    Localization: Membrane, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    UbiquitinC (UBA52 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    aa 2-76 of NP_003324.1

    Promoter

    CMV

    Available since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

36. mEmerald-UbiquitinC-N-18

    Plasmid

    #54295

    Purpose

    Localization: Membrane , Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    UbiquitinC (UBA52 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    aa 2-76 of NP_003324.1

    Promoter

    CMV

    Available since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

37. mCherry-UbiquitinC-C-18

    Plasmid

    #55149

    Purpose

    Localization: Membrane, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    UbiquitinC (UBA52 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    aa 2-76 of NP_003324.1

    Promoter

    CMV

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

38. mVenus-UbiquitinC-N-18

    Plasmid

    #56339

    Purpose

    Localization: Membrane , Excitation: 515, Emission: 528

    Depositor

    Michael Davidson

    Insert

    UbiquitinC (UBA52 Human)
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    aa 2-76 of NP_003324.1

    Promoter

    CMV

    Available since

    Dec. 18, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. mVenus-UbiquitinC-C-18

    Plasmid

    #56393

    Purpose

    Localization: Membrane, Excitation: 515, Emission: 528

    Depositor

    Michael Davidson

    Insert

    UbiquitinC (UBA52 Human)
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    aa 2-76 of NP_003324.1

    Promoter

    CMV

    Available since

    Dec. 18, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

40. mCherry-UbiquitinC-N-18

    Plasmid

    #55150

    Purpose

    Localization: Membrane , Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    UbiquitinC (UBA52 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    aa 2-76 of NP_003324.1

    Promoter

    CMV

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

41. pGC157

    Plasmid

    #19682

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Prpl-28
    

    Use

    Entry clone

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pAAV-IV-3xFLAG/HA/NBL10

    Plasmid

    #110568

    Purpose

    Cre-dependently expresses large ribosomal subunit protein RPL10A with a 3xFLAG/HA tag

    Depositor

    Alexander Nectow, Jeffrey Friedman

    Insert

    3xFLAG/HA-tagged anti-GFP VHH
    

    Use

    AAV, Cre/Lox, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pGC114

    Plasmid

    #19622

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Prpl-28<1xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)<1xNLS-lacZ::unc-54(3’)
    

    Use

    Flp/frt

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. Rp51a_SUS1 5'SS

    Plasmid

    #100991

    Purpose

    Contains model Rp51a with a GGTAtGT->tGTAtGa 5'SS mutant (SUS1 5'SS sequence).

    Depositor

    Aaron Hoskins

    Article

    Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

    Insert

    RP51A (RPS17A Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    GGTAtGT->tGTAtGa 5'SS mutant

    Promoter

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. Rp51a_No_BP-pUC18

    Plasmid

    #100992

    Purpose

    Contains model Rp51a with a TACTAAC->GTTAGTG BP mutataion

    Depositor

    Aaron Hoskins

    Article

    Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

    Insert

    RP51A (RPS17A Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    TACTAAC->GTTAGTG BP mutataion

    Promoter

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pLG-PLint

    Plasmid

    #33145

    Depositor

    Michael Rosbash

    Article

    Legrain et al Cell. 1989 May 19;57(4):573-83.

    Insert

    RP51A-synthetic minimal intron (RPS17A Budding Yeast)
    

    Use

    Tags

    LacZ

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

47. pG1AK-mCherry

    Plasmid

    #71737

    Purpose

    Modular shuttle vector for Geobacillus and E. coli

    Depositor

    Tom Ellis

    Article

    Reeve et al ACS Synth Biol. 2016 Jul 1.

    Insert

    mCherry
    

    Use

    Synthetic Biology; Shuttle vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pRplsWT

    Available since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. YIplac204-pOst1-ESCargo(Crimson)

    Plasmid

    #140154

    Purpose

    Expresses a vacuolar cargo for yeast at the TRP1 locus

    Depositor

    Benjamin Glick

    Article

    Casler et al Elife. 2020 Jun 25;9. pii: 56844. doi: 1

    Insert

    pOst1-APVNTT-DsRed-Express2-FKBP(FV)C22V-QRPL
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TPI1

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pRRP51A+intron-LacZ (pHZ18)

    Plasmid

    #33131

    Depositor

    Michael Rosbash

    Article

    Teem et al Proc Natl Acad Sci U S A. 1983 Jul;80(14

    Insert

    RP51A+intron-LacZ (RPS17A Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

50. pGST2-Ub CPS (8-16)

    Plasmid

    #17631

    Depositor

    James Hurley

    Article

    Lee et al Nat Struct Mol Biol. 2006 Mar . 13(3):26

    Insert

    Ub CPS (8-16) (RPL40A Budding Yeast)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 28, 2008

    Availability

    Academic Institutions and Nonprofits only