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Showing: 201 - 220 of 12488 results

  9. lentiCRISPR - Amplicon, BCR/ABL sgRNA 2

    Plasmid
    #70658
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic BCR/ABL-targeting sgRNA element from U6 promoter. Lentiviral backbone.
    Depositor
    David Sabatini
    Article
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against BCR/ABLamplicon found in CML-derived K562 cell line
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 28, 2016
    Availability
    Academic Institutions and Nonprofits only

  10. pcDNA3 mCherry LIC cloning vector (6B)

    Plasmid
    #30125
    Purpose
    Empty LIC vector; adds an mCherry gene to the C-terminus of your protein of interest.
    Depositor
    Scott Gradia
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 9, 2011
    Availability
    Academic Institutions and Nonprofits only
    30125-plasmid-map-sequence-id-174010

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  11. pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q23

    Plasmid
    #111723
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Article
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q23 (polyQ repeat)
    Promoter
    CMV and P10
    Available since
    Aug. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    111723-plasmid-map-sequence-id-220473

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  12. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q145

    Plasmid
    #111731
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Article
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q145 (polyQ repeat)
    Promoter
    CMV and P10
    Available since
    Nov. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    111731-plasmid-map-sequence-id-226895

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  13. pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q139

    Plasmid
    #111738
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Article
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q139 (polyQ repeat)
    Promoter
    CMV and P10
    Available since
    June 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    111738-plasmid-map-sequence-id-247719

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  14. pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q19

    Plasmid
    #111733
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Article
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q19 (polyQ repeat)
    Promoter
    CMV and P10
    Available since
    Aug. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    111733-plasmid-map-sequence-id-304484

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  15. pSBtet rtTA G72V SE Δsplice GP Luc

    Plasmid
    #194328
    Purpose
    Tetracycline inducible expression of Luciferase enzyme in mammalian cells with inclusion of rtTA G72V mutation, splice site and high sensitivity mutations for increased promotor tightness
    Depositor
    Alexander McLellan
    Article
    Saunderson et al Genes (Basel). 2022 Sep 20;13(10). pii:
    Insert
    Luciferase
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Tet-responsive promotor related mutations: G72V i…
    Promoter
    Tight tet-responsive promotor
    Available since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    194328-plasmid-map-sequence-id-383152

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  16. pPaGE Pyl TAG FliC T248TAG

    Plasmid
    #160089
    Purpose
    Encodes for MmPyl tRNA synthetase/tRNA pair and for flagellin protein (FliC) with TAG mutation at site 248, for unnatural amino acid incorporation into the flagellin protein in Pseudomonas aeruginosa.
    Depositor
    Lital Alfonta
    Article
    Ozer et al Sci Adv. 2021 Jun 11;7(24). pii: 7/24/ea
    Inserts
    Pyrrolysyl tRNA(cua) (Methanosarcina mazei)
    Pyrrolysyl tRNA synthetase (Methanosarcina mazei)
    Flagellin
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Changed Threonine 248 to TAG stop-codon.
    Promoter
    Pseudomonas aeruginosa Leu-tRNA native promoter a…
    Available since
    June 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160089-plasmid-map-sequence-id-314779

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  17. pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q51

    Plasmid
    #111734
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Article
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q51 (polyQ repeat)
    Promoter
    CMV and P10
    Available since
    Aug. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    111734-plasmid-map-sequence-id-220474

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  19. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q23

    Plasmid
    #111726
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Article
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q23 (polyQ repeat)
    Promoter
    CMV and P10
    Available since
    June 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    111726-plasmid-map-sequence-id-212896

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  20. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q54

    Plasmid
    #111727
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Article
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q54 (polyQ repeat)
    Promoter
    CMV and P10
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    111727-plasmid-map-sequence-id-272590

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Showing: 201 - 220 of 12488 results