59,469 results

1. pcDNA3-IGFBP5-V5

   Plasmid

   #11608

   Depositor

   Steven Johnson

   Insert

   Insulin Growth Factor Binding Protein 5 (IGFBP5 Human)
   

   Tags

   His and V5

   Expression

   Mammalian

   Mutation

   no mutations

   Available Since

   July 18, 2007

   Availability

   Academic Institutions and Nonprofits only

2. pSLQ1651-sgTelomere(F+E)

   Plasmid

   #51024

   Purpose

   Lentiviral vector that contains an optimized S. pyogenes sgRNA targeting human telomeres

   Depositor

   Bo Huang, Stanley Qi

   Article

   Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

   Insert

   Optimized sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mouse U6 promoter

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. AICSDP-8:TOMM20-mEGFP

   Plasmid

   #87423

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human TOMM20

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

   Insert

   TOMM20 Homology Arms with linker-mEGFP (TOMM20 Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mEGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   March 15, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. PB-TO-hNIL BSD-mApple

   Plasmid

   #182313

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression, bsd selection, mApple

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Inserts

   hNGN2 (NEUROG2 Human)
   hISL1 (ISL1 Human)
   hLHX3 (LHX3 Human)
   bsd-T2A-mycNLS-mApple
   

   Tags

   T2A-mycNLS-mApple

   Expression

   Mammalian

   Available Since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-TLR9-YFP

   Plasmid

   #13642

   Depositor

   Doug Golenbock

   Insert

   Toll-Like Receptor 9 (TLR9 Human)
   

   Tags

   YFP

   Expression

   Mammalian

   Available Since

   June 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. TetON-shYAP

   Plasmid

   #115667

   Purpose

   Inducible YAP knockdown

   Depositor

   Joan Massague

   Article

   Er et al Nat Cell Biol. 2018 Aug;20(8):966-978. d

   Insert

   YAP shRNA (YAP1 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Oct. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223_MYC_WT

   Plasmid

   #82927

   Purpose

   Gateway Donor vector containing MYC, part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, Matthew Meyerson, David Root

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   Myc (MYC Human)
   

   Use

   Gateway entry vector

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. METTL3 shRNA 1 tet pLKO puro

   Plasmid

   #162985

   Purpose

   Tet-inducible shRNA targeting human METTL3 #1

   Depositor

   Mark Rubin

   Article

   Cotter et al Mol Cancer Res. 2021 Jun 4. pii: 1541-77

   Insert

   methyltransferase like 3 (METTL3 Human)
   

   Use

   Lentiviral

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV5-Flag-RhoA wt

   Plasmid

   #11750

   Depositor

   Jeff Wrana

   Article

   Ozdamar et al Science. 2005 Mar 11. 307(5715):1603-9.

   Insert

   RhoA (RHOA Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Aug. 4, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

10. Fuw-dCas9-Tet1CD

    Plasmid

    #84475

    Purpose

    Lentiviral construct to express dCas9 fused with the catalytic domain of Tet1

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

    Insert

    dCas9-Tet1CD
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. HOP-flash

    Plasmid

    #83467

    Purpose

    TEAD-YAP/TAZ tx activity reporter. 8xwt TEAD binding sites with minimal promoter plus luciferase reporter gene

    Depositor

    Barry Gumbiner

    Article

    Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

    Insert

    Multimerized TEAD binding sites in the promoter of CTGF with minimal promoter (CCN2 Human)
    

    Use

    Luciferase

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMIEG3-JunDN

    Plasmid

    #40350

    Depositor

    Alexander Dent

    Article

    Wang et al J Immunol. 2005 Feb 15;174(4):2098-105.

    Insert

    Jun dominant negative (JUN Human)
    

    Use

    Retroviral

    Tags

    HA and IRES eGFP

    Expression

    Mammalian

    Mutation

    first 122 amino acids deleted

    Available Since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. 6xHis-SNAP-PI(4,5)P2 Probe

    Plasmid

    #211513

    Purpose

    For expression of recombinant biosensor for detection of Phosphatidylinositol-4,5-Bisphosphate (PI(4,5)P2) from bacteria

    Depositor

    Hannes Maib, David Murray

    Article

    Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

    Insert

    PLCdelta1-PH
    

    Tags

    6xhis-SNAP

    Expression

    Bacterial

    Available Since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAD29_hIRG1_4-461_pvp008

    Plasmid

    #124843

    Purpose

    Expresses human CAD (Irg1) in E.coli

    Depositor

    Konrad Buessow

    Article

    Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

    Insert

    cis-aconitate decarboxylase (ACOD1 Human)
    

    Tags

    StrepTag and TEV site

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-3 and 462-end.

    Promoter

    T7

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pF151 pcDNA3.1(+)SOD1WT

    Plasmid

    #26397

    Depositor

    Elizabeth Fisher

    Article

    Stevens et al PLoS One. 2010 . 5(3):e9541.

    Insert

    SOD1 (SOD1 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. FU-tet-o-hc-myc

    Plasmid

    #19775

    Depositor

    Konrad Hochedlinger

    Article

    Maherali et al Cell Stem Cell. 2008 Sep 11. 3(3):340-5.

    Insert

    c-myc (MYC Human)
    

    Use

    Lentiviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    T58A

    Available Since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE-ERBB3

    Plasmid

    #116735

    Purpose

    Lentiviral expression of ERBB3

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    ERBB3 (ERBB3 Human)
    

    Use

    Lentiviral

    Mutation

    WT

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. EB3-mNeonGreen

    Plasmid

    #191761

    Purpose

    Expression of mNeonGreen fused to EB3

    Depositor

    Daniel Gerlich

    Article

    Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

    Insert

    EB3-mNeonGreen (MAPRE3 Human)
    

    Tags

    mNeonGreen

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-EGFP-RhoA-wt

    Plasmid

    #12965

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    RhoA (RHOA Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. NFAT luciferase reporter

    Plasmid

    #10959

    Depositor

    Toren Finkel

    Article

    Ichida et al J Biol Chem. 2001 Feb 2. 276(5):3524-30.

    Insert

    IL-2 promoter (IL2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Available Since

    Jan. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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