We narrowed to 11,959 results for: pcDNA

1. pcDNA3 Mito-Ras-LOCKR-S

   Plasmid

   #216501

   Purpose

   FRET-based reporter for Ras activity, mitochondria localized

   Depositor

   David Baker

   Article

   Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

   Insert

   Mito-Ras-LOCKR-S
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1/NDUFV2/myc-His

   Plasmid

   #127491

   Purpose

   Expresses myc-tagged NDUFV2 in mammalian cells

   Depositor

   Shashi Jain, Dieter Wolf

   Article

   Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

   Insert

   NDUFV2 (NDUFV2 Human)
   

   Use

   Tags

   myc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1-StrepHA-SUMO2(Q87R)

   Plasmid

   #66868

   Purpose

   Mammalian expression of SUMO2(Q87R) with a strep and an HA tag

   Depositor

   Ying Liu

   Article

   Bursomanno et al J Proteome Res. 2015 Apr 3;14(4):1687-99

   Insert

   SUMO2 (SUMO2 Human)
   

   Use

   Tags

   HA and Strep

   Expression

   Mammalian

   Mutation

   Q87R

   Promoter

   CMV

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1-mCherry-CyrCR1.miR16

   Plasmid

   #128747

   Purpose

   Expresses mCherry in mammalian cells. ~200 nt of Cyrano with miR-7 site replaced by miR-16 site inserted in mCherry 3'UTR.

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   Cyrano conserved region (CR1) with miR-16 site replacing miR-7 site (OIP5-AS1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. hE-cadherin/α-catenin fusion-pcDNA3

   Plasmid

   #45771

   Depositor

   Barry Gumbiner

   Article

   Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

   Insert

   hE-cadherin/Xe α-catenin fusion (CDH1, ctnna1 Frog, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Human E-cadherin (aa 1-801) fused to Xenopus α-ca…

   Promoter

   CMV

   Available since

   June 14, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pcDNAI Neo human PECAM FL

   Plasmid

   #42902

   Depositor

   Steven Albelda

   Insert

   PECAM (PECAM1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 20, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3puro-mGFP-FMR1iso1-G266E

   Plasmid

   #225468

   Purpose

   Express mEGFP-fusion protein of FMR1 isoform 1 (G266E)

   Depositor

   Christine Mayr

   Article

   Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

   Insert

   FMR1 isoform 1 (FMR1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   G266E

   Promoter

   CMV

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3puro-mGFP-FMR1iso1-I304N

   Plasmid

   #225463

   Purpose

   Express mEGFP-fusion protein of FMR1 isoform 1 (I304N)

   Depositor

   Christine Mayr

   Article

   Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

   Insert

   FMR1 isoform 1 (FMR1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   I304N

   Promoter

   CMV

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1-mouse cdk6 D163N

   Plasmid

   #75173

   Purpose

   Mammalian expression of mouse cdk6 with D163N

   Depositor

   Martine Roussel

   Insert

   cyclin dependent kinase 6 (Cdk6 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D163N

   Promoter

   CMV

   Available since

   May 12, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA-c/o NL4-3 Env delta CT

    Plasmid

    #139285

    Purpose

    HIV-1 NL4-3 glycoprotein lacking cytoplasmic tail

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    codon optimized HIV-1 Env delta cytoplasmic tail
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deletion of cytoplasmic tail after residue 710

    Promoter

    CMV IE

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA5/FRT/TO HIS HSPH1

    Plasmid

    #19534

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    heat shock 105kDa/110kDa protein 1 (HSPH1 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1(-) rat C/EBP alpha

    Plasmid

    #12550

    Depositor

    Peter Johnson

    Article

    Miller et al J Biol Chem. 2003 Apr 25;278(17):15178-8

    Insert

    CCAAT/Enhancer-binding Protein alpha (Cebpa Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA-LBR-EGFP-polyA

    Plasmid

    #59748

    Purpose

    Express LBR-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. This LBR contains N-terminal domain only.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    LBR-EGFP
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Yellow Cameleon-Nano140/pcDNA3

    Plasmid

    #51966

    Purpose

    mammalian expression of Yellow Cameleon-Nano140, an ultrasensitive Ca2+ indicator with Kd = 140 nM

    Depositor

    Takeharu Nagai

    Article

    Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

    Insert

    Yellow Cameleon-Nano140
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA-EGFP-ELYS-polyA

    Plasmid

    #59746

    Purpose

    Express EGFP-ELYS in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    EGFP-ELYS
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCDNA5-TEX264-deltaLRR-cSSH

    Plasmid

    #220210

    Purpose

    Mammalian expression of TEX264, 2-25 residues (LRR) truncated, C-terminal twin-Strep_HA tag

    Depositor

    Kristijan Ramadan

    Article

    Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

    Insert

    TEX264 (TEX264 Human)
    

    Use

    Tags

    Strep-strep-HA

    Expression

    Mammalian

    Mutation

    c.4-75del / p.2-24del

    Promoter

    CMV/TetO

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCDNA/golgin-160/myc

    Plasmid

    #180632

    Purpose

    Expression of WT golgin-160

    Depositor

    Carolyn Machamer

    Article

    Hicks et al J Biol Chem. 2002 Sep 27. 277(39):35833-

    Insert

    golgin-160 (GOLGA3 Human)
    

    Use

    Tags

    Myc-His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDNA3 Flag Jnk2a2(apf)

    Plasmid

    #13761

    Depositor

    Roger Davis

    Article

    Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

    Insert

    Jnk2a2(apf) (MAPK9 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    replaced dual phosporylation motif THr(183)-Pro-T…

    Promoter

    Available since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3 CDK9 (D167N) HA (P#811)

    Plasmid

    #14641

    Depositor

    Matija Peterlin

    Article

    Fujinaga et al J Virol. 1998 Sep . 72(9):7154-9.

    Insert

    cdk9 D167N (CDK9 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D167N. Kinase negative mutant. Please see deposit…

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA-emerin-EGFP-polyA

    Plasmid

    #59747

    Purpose

    Express emerin-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    emerin-EGFP
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA5/FRT/TO-GAlpha12-RLuc8

    Plasmid

    #140985

    Purpose

    Encodes a G alpha subunit (GNA12) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlpha12-RLuc8 (GNA12 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3.1-mas-KB-1753-Nluc

    Plasmid

    #158603

    Purpose

    Plasmid expressing KB-1753-Nluc in mammalian cells

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker(GIKLGG) - KB1753 - linker (GGTGGS) - Nluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pCDNA4TO-3XFLAG Halo WT nsp1

    Plasmid

    #175412

    Purpose

    Expressing WT CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV2 nsp1 (ORF1ab CoV2)
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1(+) EGFP-Rac1(G12C)

    Plasmid

    #224279

    Purpose

    Cell transfection and expression of Rac1 (G12C)

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    Rac1 (RAC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G12C

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3 CDK7 D155A HA (P#812)

    Plasmid

    #14648

    Depositor

    Matija Peterlin

    Article

    Kanazawa et al Oncogene. 2003 Aug 28. 22(36):5707-11.

    Insert

    Cdk7 (CDK7 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D155A: dominant negative

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

26. pcdna6.2- myc ULK1 k46I

    Plasmid

    #27630

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2011 Jan 28;331(6016):456-61. d

    Insert

    mouse myc ULK1 K46I (Myc Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Initial M from ULK1 removed. Silent mutation at A…

    Promoter

    Available since

    Feb. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

27. pcDNA3.1-CD81-(TEV)-tTA

    Plasmid

    #162598

    Purpose

    human CD81-TEV cleavage site-tTA

    Depositor

    Masaharu Somiya

    Article

    Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

    Insert

    human CD81 (CD81 Human)
    

    Use

    Tags

    TEV protease cleavage site and tTA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1(+) prME (JEV) sdm2

    Plasmid

    #52881

    Purpose

    expression of Japanese Encephalitis Virus VLPs in mammalian cells

    Depositor

    Yorgo Modis

    Article

    Nour et al PLoS Pathog. 2013;9(9):e1003585. doi: 10

    Insert

    prME (POLY Japanese encephalitis virus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    sdm2

    Promoter

    CMV

    Available since

    May 14, 2014

    Availability

    Academic Institutions and Nonprofits only

29. pcDNA5 FRT-TO-EGFP-UPF1-WT

    Plasmid

    #136000

    Purpose

    DOX-inducible WT UPF1 inserted with GFP tagged on the N-terminus and used with the Flp-In T-Rex System

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    UPF1
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1 His-V5 Myc dMBIV

    Plasmid

    #176055

    Purpose

    Expresses Myc Mutant (delta Myc BoxIV) tagged with His and V5 in Mammalian cells

    Depositor

    Linda Penn

    Article

    Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

    Insert

    Myc
    

    Use

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    deleted amino acids 304-324

    Promoter

    CMV

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3.1 His-V5 Myc dMBIIb

    Plasmid

    #176054

    Purpose

    Expresses Myc Mutant (delta Myc BoxIIb) tagged with His and V5 in Mammalian cells

    Depositor

    Linda Penn

    Article

    Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

    Insert

    Myc
    

    Use

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    deleted amino acids 259-270

    Promoter

    CMV

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3.1 His-V5 Myc dMBIIa

    Plasmid

    #176053

    Purpose

    Expresses Myc Mutant (delta Myc BoxIIa) tagged with His and V5 in Mammalian cells

    Depositor

    Linda Penn

    Article

    Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

    Insert

    Myc
    

    Use

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    deleted amino acids 188-199

    Promoter

    CMV

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3.1 His-V5 Myc dMBII

    Plasmid

    #176052

    Purpose

    Expresses Myc Mutant (delta Myc BoxII) tagged with His and V5 in Mammalian cells

    Depositor

    Linda Penn

    Article

    Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

    Insert

    Myc
    

    Use

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    deleted amino acids 128-143

    Promoter

    CMV

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3.1 His-V5 Myc dMBI

    Plasmid

    #176051

    Purpose

    Expresses Myc Mutant (delta Myc BoxI) tagged with His and V5 in Mammalian cells

    Depositor

    Linda Penn

    Article

    Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

    Insert

    Myc
    

    Use

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    deleted amino acids 44-63

    Promoter

    CMV

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3.1 His-V5 Myc dMB0

    Plasmid

    #176050

    Purpose

    Expresses Myc Mutant (delta Myc Box0) tagged with His and V5 in Mammalian cells

    Depositor

    Linda Penn

    Article

    Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

    Insert

    Myc
    

    Use

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    deleted amino acids 16-33

    Promoter

    CMV

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3.1/Puro-CAG-VSFP-CR

    Plasmid

    #40257

    Depositor

    Michael Lin

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    VSFP-CR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA4-Beclin1-(151-241)

    Plasmid

    #24393

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

    Insert

    Beclin1 (BECN1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Amino acids 151-241 are deleted. Delta CC mutant.

    Promoter

    Available since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

38. pcDNA5 FRT TO GFP11-LRRK2_G2019S

    Plasmid

    #231175

    Purpose

    Expresses GFP-11-LRRK2 G2019S in mammalian cells

    Depositor

    Samara Reck-Peterson

    Article

    Raig et al bioRxiv [Preprint]. 2025 Feb 13:2024.09.

    Insert

    GFP11-LRRK2 (G2019S) (LRRK2 Human)
    

    Use

    Tags

    GFP11

    Expression

    Mammalian

    Mutation

    G2019S

    Promoter

    CMV

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3-Nuc-ExRai-AMPKAR

    Plasmid

    #192450

    Purpose

    ExRai-AMPKAR targeted to the nucleus.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    ExRai-AMPKAR-NLS
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3-Casp2 C303A-Flag

    Plasmid

    #11812

    Depositor

    Guy Salvesen

    Insert

    Caspase-2 catalytic mutant (CASP2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    C303A

    Promoter

    Available since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only